Mechanism of Sanhua Decoction in the Treatment of Ischemic Stroke Based on Network Pharmacology Methods and Experimental...
... cytoplasmic vesicle lumen (GO: 0060205), vesicle lumen (GO: 0031983), RNA polymerase II transcription factor complex (GO: ... Then, a small cut was made at the bifurcation of ICA and CCA, the end of the threaded bolt was inserted along the incision, and ... long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs) participate in RNA-mediated networks through complex mechanisms, ... X. C. Duan, L. Han, D. Y. Peng, L. Xiao, Q. Bao, and H. Peng, "Bioinformatics analysis of a long non-coding RNA and mRNA ...
Acupuncture May Exert Its Therapeutic Effect through MicroRNA-339/Sirt2/NFB/FOXO1 Axis
P. Landgraf, M. Rusu, R. Sheridan et al., "A mammalian microRNA expression atlas based on small RNA library sequencing," Cell, ... 70 nucleotide stem-loop precursors and subsequently processed by the cytoplasmic RNase-III type enzyme Dicer to generate ~22 ... Z. Mourelatos, "Small RNAs: the seeds of silence," Nature, vol. 455, no. 7209, pp. 44-45, 2008. ... MicroRNAs (miRNAs) is a large family of endogenous, small noncoding RNAs with 20-25 nucleotides that have emerged as key ...
PRKRA Localizes to Nuage Structures and the Ectoplasmic Specialization and Tubulobulbar Complexes in Rat and Mouse Testis
The results suggest that PRKRA functions in the nuage as an element of RNA silencing system and plays unknown role in the ... The cytoplasmic RNA-induced silencing complex (RISC) contains dsRNA binding proteins, including PRKRA, TRBP, and Dicer. RISC ... PRKRA is contained in the cytoplasmic RNA-induced silencing complex (RISC) together with transactivation response RNA binding ... O. Meikar, M. Da Ros, H. Korhonen, and N. Kotaja, "Chromatoid body and small RNAs in male germ cells," Reproduction, vol. 142, ...
MicroRNA-34a: A Key Regulator in the Hallmarks of Renal Cell Carcinoma
Total RNA, including the small RNAs, was isolated from FFPE tissue sections (5 to 8 μm thick) using miRNeasy FFPE Kit (Qiagen, ... Clear cell RCC (Ng1) weakly expresses bcl2 (x100), (photo 1), clear cell RCC (Ng2) with focal strong cytoplasmic bcl2 (x100) ( ... This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in ... values of RNU6B small RNA and GAPDH were uniformly and stably expressed with no significant difference between cancer and ...
Assessment of the Carbon Monoxide Metabolism of the Hyperthermophilic Sulfate-Reducing Archaeon Archaeoglobus fulgidus VC-16 by...
Overall, carboxidotrophic growth seems as an intrinsic capacity of ,i,A. fulgidus,/i, with little need for novel resistance or ... RNA extraction was performed as described in the previous section. The cDNA synthesis was performed using the High Capacity RNA ... The electrons from cytoplasmic oxidation of CO may thus flow from the nitrate reductase-like respiratory complex via ... In contrast, the monofunctional CODH (CooS) appears to play a smaller role in CO metabolism. Transcripts of cooS was detected ...
Surgical Removal of a Canine Aortic Thromboembolism Secondary to Pancreatitis
A. Boswood, C. R. Lamb, and R. N. White, "Aortic and iliac thrombosis in six dogs," Journal of Small Animal Practice, vol. 41, ... and RNA damage, lipid peroxidation of cell membranes, and loss of cell membrane permeability [29, 30]. Neutrophils are ... leading to cytoplasmic swelling and lysis and the initiation of the proapoptotic caspase and calpain cascades [29]. Massive ... C. G. Ruaux, "Diagnostic approaches to acute pancreatitis," Clinical Techniques in Small Animal Practice, vol. 18, no. 4, pp. ...
Artificial Box C/D RNAs Affect Pre-mRNA Maturation in Human Cells
O-ribose methylation of nucleotides in eukaryotic ribosomal RNAs and small nuclear RNAs. Recently snoRNAs are predicted to ... The artificial box C/D RNA directed to the branch point adenosine of the second intron, as well as the analogue directed to the ... Inasmuch as artificial box C/D RNAs decreased viability of transfected human cells, we propose that natural snoRNAs as well as ... in this study we tested the ability of artificial box C/D RNAs to affect the maturation of target pre-mRNA. We found that ...
Identification of Hub Genes Associated with Lung Adenocarcinoma Based on Bioinformatics Analysis
... and small bubble, plays a role, and the cytoplasmic matrix in the tumor actually adjusts all aspects of the tumor cells and ... M. E. Ritchie, B. Phipson, D. Wu et al., "Limma powers differential expression analyses for RNA-sequencing and microarray ... X. Lei, L. Du, P. Zhang et al., "Knockdown GTSE1 enhances radiosensitivity in non-small-cell lung cancer through DNA damage ... T. K. P. Blee, N. K. Gray, and M. Brook, "Modulation of the cytoplasmic functions of mammalian post-transcriptional regulatory ...
Early Postweaning Treatment with Dimethyl Fumarate Prevents Prenatal Dexamethasone- and Postnatal High-Fat Diet-Induced...
... small,-arginine-to-ADMA ratio, and activation of genes related to nutrient sensing and autophagy (e.g., ,i,Pparb,/i,, ,i,Pparg ... RNA was extracted from the kidney cortex according to previously described methods [15]. In addition to Nrf2, several genes ... Immunostaining of both cytoplasmic and nuclear 8-OHdG in the glomeruli and renal tubules indicated intense staining in the DEX ... and little staining in the control (8 ± 1 positive cells) and DEX + HF + DMF (17 ± 5 positive cells) groups (Figure 2). ...
Production of Polyclonal Antibody against Interleukin-33 and Assessment of Its Distribution in Murine Liver and Lung
Total cellular RNA was extracted from spinal cord of BALB/c mouse according to Trizol Reagent instructions. The coding region ... very little information is available on the expression of IL-33 protein in liver and lung tissues. To our knowledge this is the ... Antibodies showed nuclear or cytoplasmic staining. Furthermore, there was a significant increase of IL-33 expression in ...
High Mobility Group B Proteins, Their Partners, and Other Redox Sensors in Ovarian and Prostate Cancer
J. Bektic, K. Pfeil, A. P. Berger et al., "Small G-protein RhoE is underexpressed in prostate cancer and induces cell cycle ... H. R. Gatla, B. Singha, V. Persaud, and I. Vancurova, "Evaluating cytoplasmic and nuclear levels of inflammatory cytokines in ... M. Gnanasekar, S. Thirugnanam, and K. Ramaswamy, "Short hairpin RNA (shRNA) constructs targeting high mobility group box-1 ( ... F. Katsuoka, H. Motohashi, T. Ishii, H. Aburatani, J. D. Engel, and M. Yamamoto, "Genetic evidence that small Maf proteins are ...
NRF1 and NRF2 mRNA and Protein Expression Decrease Early during Melanoma Carcinogenesis: An Insight into Survival and MicroRNAs
MicroRNAs (miRNAs) are small noncoding RNAs that posttranscriptionally regulate gene expression by imperfect matching of mRNA [ ... Nuclear and cytoplasmic NRF1 correlated positively in the complete series of samples (. , 219 samples, Figure 4). Nuclear and ... A. S. Nikitina, E. I. Sharova, S. A. Danilenko et al., "Novel RNA biomarkers of prostate cancer revealed by RNA-seq analysis of ... cytoplasmic NRF1 and p40-NRF2 had a positive correlation in the complete series of samples (. and , and , respectively). Also, ...
Identification and Validation of Autophagy-Related Gene Nomograms to Predict the Prognostic Value of Patients with Cervical...
Autophagy is a process of engulfing ones own cytoplasmic proteins or organelles and coating them into vesicles, fusing with ... Autophagy is a process of engulfing ones own cytoplasmic proteins or organelles and coating them into vesicles, fusing with ... M. E. Ritchie, B. Phipson, D. Wu et al., "Limma powers differential expression analyses for rna-sequencing and microarray ... "Development and validation of a nomogram for predicting survival in patients with resected non-small-cell lung cancer," Journal ...
SNHG5/miR-299-5p/ATF2 Axis as a Biomarker in Immune Microenvironment of Intervertebral Disc Degeneration
In order to improve the reliability of research results, coding RNA and noncoding RNA data from the same sample set were ... cytoplasmic vesicle membrane, cytoplasmic vesicle, and myelin sheath in cellular component (CC) (Figure 4(a)); and mainly ... Studies have shown that the main biological change of IDD is the massive loss of proteoglycans, especially small molecules of ... W. Guo, B. Zhang, C. Sun et al., "Circular RNA derived from TIMP2 functions as a competitive endogenous RNA and regulates ...
Targeting Early Atherosclerosis: A Focus on Oxidative Stress and Inflammation
... nanoparticles or viral vectors can deliver small interfering RNAs (siRNAs) to silence molecules involved in endothelial ... The NLRP3 inflammasome is a cytoplasmic complex present in immune cells such as monocytes and neutrophils that detect dangerous ... Even small amounts of activity have protective results, and performing regular physical activity reduces the risk of CVD and ... M. T. Hamilton, G. N. Healy, D. W. Dunstan, T. W. Zderic, and N. Owen, "Too little exercise and too much sitting: inactivity ...
Adjudication of the Alleged Role of Vitamin D in the Antimicrobial Pathway
In a small study involving 126 untreated patients with TB and 116 healthy contacts who were sensitized, but did not have any ... Specific inhibition at the level of messenger RNA," The Journal of Clinical Investigation, vol. 79, no. 6, pp. 1659-1664, 1987. ... it had been postulated that both hormones interacted with specific cytoplasmic receptors in the leukemic cells [44]. ... Although the association between low vitamin D levels and mortality was not statistically significant, the relatively small ...
Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes...
... especially the geranylgeranylation of small GTPase, Rac1. Our previous studies found that GGTase-I played an important role in ... and small GTP-binding protein Rac1 [27, 28]. The activation of NOX is initiated by translocation of the cytoplasmic components ... Cas9 mRNA, guide RNA, and donor vectors were microinjected into the fertilized eggs of C57BL/6J mice. The resulting homologous ... Knockout of VSMC GGTase-I Inhibits the Activation of Small GTPase, Rac1. The activation of small GTPase, Rac1, depends on its ...
Comprehensive Computational Analysis of Honokiol Targets for Cell Cycle Inhibition and Immunotherapy in Metastatic Breast...
A. J. Ridley, H. F. Paterson, C. L. Johnston, D. Diekmann, and A. Hall, "The small GTP-binding protein rac regulates growth ... in which the database utilized data from GEO or RNA-seq libraries from The Cancer Genome Atlas (TCGA), Therapeutically ... and HSP90AB1 were overexpressed in the cytoplasmic/membranous region (Figure 4(b)). Finally, CASP9 was not differentially ... J. Bertran-Alamillo, V. Cattan, M. Schoumacher et al., "AURKB as a target in non-small cell lung cancer with acquired ...
Pathomechanisms of Blood-Brain Barrier Disruption in ALS
Small molecules, such as miRNA, can not only lead the way in identifying novel biomarkers, but may also explain how the gene ... Similarly, adherence junctions (AJs) are more basal than TJs and are defined by their cytoplasmic face being linked to the ... Mainly the protein processing in the endoplasmic reticulum and RNA transport pathways were activated upon exposure of cells to ... Little is known regarding the prevalence and prognosis of people with cerebrospinal microbleeds. This is due to the diagnosis ...
Involvement of Nrf2 in Ocular Diseases
1) Small Molecule Nrf2 Activators. Many studies have used Nrf2 pathway activating drugs to evaluate the cytoprotective role of ... Oxidative stress can denature lipids and proteins [10, 12-14], as well as induce DNA and RNA fragmentation [15-17], leading to ... Regulated by Nrf2 activation, the Grx family is composed of two specific subsets: the cytoplasmic Grx1 and the primarily ... showed that a small amount of H2O2 enhances rabbit corneal epithelial cell viability, migration, adhesion, and attachment to ...
The Pathology of T Cells in Systemic Lupus Erythematosus
chain) are the longest cytoplasmic chains amongst the rest. The cytoplasmic portions of and chains are critically involved in ... V. R. Moulton, V. C. Kyttaris, Y.-T. Juang, B. Chowdhury, and G. C. Tsokos, "The RNA-stabilizing protein HuR regulates the ... Recently, N-acetylcysteine (NAC), the precursor of glutathione, was shown in a small clinical trial that at doses 2.4 gm and ... receptors) and are anchored into the plasma membrane by a short cytoplasmic tail [9]. A minor group (15%) of TCRs comprise a ...
Emerging Roles of Impaired Autophagy in Fatty Liver Disease and Hepatocellular Carcinoma
D. Sir, C. F. Kuo, Y. Tian et al., "Replication of hepatitis C virus RNA on autophagosomal membranes," The Journal of ... However, smaller lipid droplets and lower amount of triglyceride (TG) content was observed in these samples compared to wild ... Y. Ohsaki, J. Cheng, A. Fujita, T. Tokumoto, and T. Fujimoto, "Cytoplasmic lipid droplets are sites of convergence of ... D. F. Egan, M. G. Chun, M. Vamos et al., "Small molecule inhibition of the autophagy kinase ULK1 and identification of ULK1 ...
Mizoribine: A New Approach in the Treatment of Renal Disease
triphosphate [12]. MZB, however, was found not to be incorporated into DNA or RNA in a study using [14C] MZB, but did ... Whole-body autoradiography revealed high levels of radioactivity in the stomach, small intestine, liver, kidney, spleen, and ... Their study was conducted on 5 patients, 4 with myeloperoxidase (MPO) anti-neutrophil cytoplasmic antibody- (ANCA-) associated ... and found that MZB strongly inhibited both DNA synthesis and RNA synthesis, but not protein synthesis. AZT, which also exerts ...
Cross Talk of Proteostasis and Mitostasis in Cellular Homeodynamics, Ageing, and Disease
T. Liu, B. Lu, I. Lee, G. Ondrovičová, E. Kutejová, and C. K. Suzuki, "DNA and RNA binding by the mitochondrial lon protease is ... L. Raj, T. Ide, A. U. Gurkar et al., "Selective killing of cancer cells by a small molecule targeting the stress response to ... Autophagy is an evolutionarily conserved process that is responsible for the lysosome-mediated degradation of cytoplasmic ... B. H. Kang, M. D. Siegelin, J. Plescia et al., "Preclinical characterization of mitochondria-targeted small molecule Hsp90 ...
Novel Molecular Targets Participating in Myocardial Ischemia-Reperfusion Injury and Cardioprotection
L. Lin, Z. Yang, G. Zheng et al., "Analyses of changes in myocardial long non-coding RNA and mRNA profiles after severe ... C. X. Qin, L. T. May, R. Li et al., "Small-molecule-biased formyl peptide receptor agonist compound 17b protects against ... an oxidative stress-responsive cytoplasmic adaptor molecule that is an upstream regulator of both IKK and JNK) [104, 105], ... generating a smaller infarct size and improving the systolic function of the left ventricle. Similarly, the innovative roles ...