Here we show that recombinant, human Argonaute2 can combine with a small interfering RNA (siRNA) to form minimal RISC that ... Genetic, biochemical and structural studies have implicated Argonaute proteins as the catalytic core of the RNAi effector ... Here we show that recombinant, human Argonaute2 can combine with a small interfering RNA (siRNA) to form minimal RISC that ... Genetic, biochemical and structural studies have implicated Argonaute proteins as the catalytic core of the RNAi effector ...

In the hepatitis delta virus (HDV) ribozyme, site-specific self-cleavage of the viral RNA phosphodiester backbone requires both ... Ribozymes enhance chemical reaction rates using many of the same catalytic strategies as protein enzymes. In the hepatitis ... Ribozymes enhance chemical reaction rates using many of the same catalytic strategies as protein enzymes. ... This finding implies that RNA structural rearrangements control the reactivity of ribozymes and ribonucleoprotein enzymes. ...

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM K+ ... By examining the catalytic process of Bacillus halodurans RNase H1 in crystallo, however, we found that the two canonical Mg 2+ ... Cation trafficking propels RNA hydrolysis.. Samara, N.L., Yang, W.. (2018) Nat Struct Mol Biol 25: 715-721 ... Other DNA and RNA enzymes that catalyze consecutive cleavage and strand-transfer reactions in a single active site may ...

The fundamental engines of RNA silencing are RISC and RITS complexes, whose common components are 21-25 nt RNA and an Argonaute ... align spatially with the catalytic Asp residues of RNase H-like catalytic sites, suggesting that in eukaryotic Argonaute ... The conserved region around the C-terminus of the PIWI domain, which is required for small interfering RNA (siRNA) binding to ... RNA silencing regulates gene expression through mRNA degradation, translation repression and chromatin remodelling. ...

Human nuclear exosome-MTR4 RNA complex - composite map after focused reconstruction ... The structure reveals an RNA-engaged helicase atop the non-catalytic core, with RNA captured within the central channel and ... The ribonucleolytic RNA exosome interacts with RNA helicases to degrade RNA. To understand how the 3 to 5 Mtr4 helicase ... Helicase-Dependent RNA Decay Illuminated by a Cryo-EM Structure of a Human Nuclear RNA Exosome-MTR4 Complex.. Weick, E.M., Puno ...

On the other side of the substrate, the conserved Asp69 is proposed to act as a base coordinating the catalytic water molecule ... and prevent the incorporation of hypoxanthine or xanthine into nascent DNA or RNA. Here we present the 1.5-A-resolution crystal ...

Crystal structures of catalytic complexes of the oxidative DNA/RNA repair enzyme AlkB.. Yu, B., Edstrom, W.C., Benach, J., ... Escherichia coli AlkB and the homologous human proteins ABH2 and ABH3 (refs 5, 7) promiscuously repair DNA and RNA bases ... which may enable docking of diverse alkylated nucleotide substrates in optimal catalytic geometry. Different crystal structures ... protein dynamics modulate redox chemistry and that a hypothesized migration of the reactive oxy-ferryl ligand on the catalytic ...

Aspartate transcarbamylase, with catalytic subunits in red, regulatory subunits in blue, and CTP in green. ... which are used to build nucleotides in DNA and RNA. Early studies found that Escherichia coli ATCase is regulated by the level ... The whole complex is usually in the inactive state, but the catalytic chains are cooperative: as with the cooperativity of the ... The structure revealed that ATCase is a large complex, with six catalytic chains arranged in the center, surrounded by three ...

catalytic activity, acting on RNA. *RNA exonuclease activity, producing 5-phosphomonoesters. *catalytic activity, acting on a ... catalytic activity, acting on RNA. *RNA exonuclease activity, producing 5-phosphomonoesters. *catalytic activity, acting on a ... catalytic activity, acting on RNA. *RNA exonuclease activity, producing 5-phosphomonoesters. *catalytic activity, acting on a ... catalytic activity, acting on RNA. *RNA exonuclease activity, producing 5-phosphomonoesters. *catalytic activity, acting on a ...

The Integrator endonuclease docks to the RNA exit site and opens to cleave nascent RNA about 20 nucleotides from the Pol II ... Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform. FA [auth q]. 309. Homo sapiens. Mutation(s): 0 Gene ... Integrator and protein phosphatase 2A (PP2A) form a complex that dephosphorylates paused RNA polymerase II (Pol II), cleaves ... the nascent RNA, and terminates transcription. We report the structure of the pretermination complex containing the human ...

Geis illustrates the structure of the ribonuclease S that highlights the dinucleotide RNA substrate in red and the four ... The two catalytic trimers, illustrated with tiny specks, are seen on the top and bottom, while the three regulatory dimers, ... and its associated proteins that form a preinitiation complex in all eukaryotes for transcribing DNA to messenger RNA. Acrylic ...

There are three major types of RNA: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). The various types of ... Prosthetic groups are involved in catalytic mechanisms and required for activity. They may also be called cofactor, or coenzyme ... ribose - Type of pentose sugar found in RNA. ribosomal RNA - Type of RNA that forms a component of ribosomes that is essential ... mRNA - Messenger RNA is an RNA molecule synthesized by a cell, based on genetic information encoded by DNA, that directs the ...

transcription by RNA polymerase II. *regulation of transcription by RNA polymerase II ... catalytic activity. *ubiquitin-protein transferase activity. *sequence-specific DNA binding. *sequence-specific double-stranded ...

RNA-directed RNA polymerase, catalytic domain. Domain. D [auth 4]. IPR000605. Helicase, superfamily 3, single-stranded DNA/RNA ... RNA-directed RNA polymerase, catalytic domain. Domain. A [auth 1]. IPR000605. Helicase, superfamily 3, single-stranded DNA/RNA ... RNA-directed RNA polymerase, catalytic domain. Domain. B [auth 2]. IPR000605. Helicase, superfamily 3, single-stranded DNA/RNA ... RNA-directed RNA polymerase, catalytic domain. Domain. C [auth 3]. IPR000605. Helicase, superfamily 3, single-stranded DNA/RNA ...

The PRC-barrel is an all beta barrel domain found in photosystem reaction centre subunit H of the purple bacteria and RNA ... The PRC-barrel is an all beta barrel domain found in photosystem reaction centre subunit H of the purple bacteria and RNA ... catalytic activity. *cation binding. *transition metal ion binding. *iron ion binding. *ion binding ...

It does not include the catalytic domain of dual specificity kinases.. Less Domain ... transcription by RNA polymerase II. *regulation of transcription by RNA polymerase II ... This entry represents the catalytic domain found in a number of serine/threonine- and tyrosine-protein kinases. ...

A runner-up award was made to Paul Hubbard for the poster "Structure and Catalytic Mechanism of Bacterial 2, 4 - Dienoyl CoA ... The topics covered -- including proteins, DNA, RNA, carbohydrates, and complex structures -- offer the reader a better ... clamps around the messenger RNA or the power stroke motion of myosin. I am also fascinated by the beautiful symmetry of ...

A runner-up award was made to Paul Hubbard for the poster "Structure and Catalytic Mechanism of Bacterial 2, 4 - Dienoyl CoA ... The topics covered -- including proteins, DNA, RNA, carbohydrates, and complex structures -- offer the reader a better ... clamps around the messenger RNA or the power stroke motion of myosin. I am also fascinated by the beautiful symmetry of ...

RNA polymerase II core promoter sequence-specific DNA binding. *RNA polymerase II transcription regulatory region sequence- ... negative regulation of catalytic activity. *negative regulation of nitrogen compound metabolic process ... positive regulation of transcription by RNA polymerase II. *positive regulation of transcription from RNA polymerase II ...

Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). O. d4qv7o_. Alpha and ... Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). J. d4qv7j_. Alpha and ... Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). X. d4qv7x_. Alpha and ... Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). K. d4qv7k_. Alpha and ...

Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). O. d4qzzo_. Alpha and ... Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). J. d4qzzj_. Alpha and ... Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). X. d4qzzx_. Alpha and ... Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). K. d4qzzk_. Alpha and ...

Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). O. d5cz7o_. Alpha and ... Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). B. d5cz7b_. Alpha and ... Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). W. d5cz7w_. Alpha and ... Proteasome beta subunit (catalytic). (Saccharomyces cerevisiae S288C ) [TaxId: 559292 ], SCOPe (2.08). J. d5cz7j_. Alpha and ...