RNA-seq will provide the expression variations of total RNA and small RNA in human MSCs with miR-200c overexpression. RNA-Seq ... Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing ... For RNA-. seq. 1 μg RNA and the TruSeq Stranded Total RNA Library Prep kit (Illumina) are used to perform reverse transcription ... The miRNA can be a RNA of any length as long as it is recognized and bound by a target to form a cleavable RNA duplex. Specific ...

108010003152 bacteriophage T7 RNA polymerase Proteins 0.000 description 3 * 238000005119 centrifugation Methods 0.000 ... Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes ... 0036] T7 RNA polymerase gene with lacUV5 promoter was integrated into the LacZ position in the E. coli K4 genome. Briefly, a ... Next, T7 RNA-polymerase gene was cloned into the pTKIP vector and transformed into K4 AkfoE strains with landing-pad ...

An insertion or deletion mutation using guide RNAs targeting the first exon of the transcript encoding each protein is created ... 229920002391 Guide RNA Polymers 0.000 claims abstract description 56 * 108020005004 Guide RNA Proteins 0.000 claims abstract ... Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing ... Silencing of RNA translation by short-hairpin RNA (FIG.8); (2) Modulation of endogenous gene expression by guide RNA-targeted ...

... total RNA comprising serine protease mRNA; (i) transforming the isolated total RNA of (h) into cDNA comprising serine protease ... are dependent on the presence of a mutation in the catalytic domain of the EGFR (Schilder RJ et al, Clin Cancer Res 2005; 11: ... total RNA comprising serine protease mRNA; (i) transforming the isolated total RNA of (h) into cDNA comprising serine protease ... total RNA comprising a serine protease mRNA; (c) transforming the isolated total RNA of (b) into cDNA comprising serine ...