Villin-Like Actin-Binding Proteins Are Expressed Ubiquitously in Arabidopsis | Plant Physiology
1993) The 100 kDa F-actin capping protein in Dictyostelium amoeba is a villin prototype ("protovillin"). FEBS Lett 328:71-76. ... RNA and protein analyses showed that AtVLN genes are expressed in most tissues of Arabidopsis. In addition, immunologically ... 1994) Characterisation of the F-actin binding domains of villin: classification of F-actin binding proteins into two groups ... the remainder of the protein) can bind F-actin, but they bind to different sites on the actin filament (Glenney et al., 1981; ...
The Role of Pyruvate Dehydrogenase and Acetyl-Coenzyme A Synthetase in Fatty Acid Synthesis in Developing Arabidopsis Seeds |...
... coli maltose binding protein. This recombinant protein was isolated from 1-L cultures, purified on an amylose column as per the ... Ten micrograms of RNA isolated from each Arabidopsis sample, along with a range of RNA concentration standards (0.01-10.00 pg ... CAPS), 10% (w/v) methanol, pH 10.5, using a blotting apparatus (Genie, Idea Scientific). Immunodetection of specific proteins ... The Arabidopsis ACS protein was aligned with the ACS1 and ACS2 proteins from yeast and the ACS protein from E. coli using the ...
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RNA Extraction and Preparation for RT-PCR and MAM3 Cloning. Total RNA was isolated from all tissues but siliques with Trizol ( ... The recombinant protein was detected in the bacterial extract as a prominent band of the predicted size of 53 kD by SDS-PAGE ... fully end capped, reverse-phase column (LiChrospher RP-18, 250- × 4.6-mm i.d., 5-μm particle size, Chrompack). Procedures for ... no ATP-binding site motifs are apparent that might explain the different effects of this nucleotide. However, there are ...
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1998) Low density membranes are associated with RNA-binding proteins and thylakoids in the chloroplast of Chlamydomonas ... Low-salt cushions contained 20% (w/v) Suc, 20 mm Tris-HCl, pH 7.5, 100 mm KCl, 10 mm MgCl2, 2 mm DTT, and 100 µg/mL CAP; high- ... Peptides bound by the chaperones were not bound by the unrelated mCherry protein, indicating that these peptides were ... D and E, Binding of the CrTIG1 protein N-terminal domain to the CrAtpB (D) and CrRbcL (E) peptide libraries described above. ...