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*  Pathology-Driven Comprehensive Proteomic Profiling of the Prostate Cancer Tumor Microenvironment | Molecular Cancer Research
RNA-binding protein Raly. −0.345. stain HIGH 2/11. ↑. Protein synthesis, infectious disease, RNA posttranscriptional ... Protein IDs. Protein names. t-test difference. PROTEIN ATLAS-Ab Quality. Antibody. G4 vs. G3. IPA network. ... RNA posttranscriptional modification, infectious disease, renal and urological disease. RPL23. P62829. 60S ribosomal protein ... Protein synthesis, gene expression, hematologic disease, RNA posttranscriptional modification. H1FX. Q92522. Histone H1x. − ...
http://mcr.aacrjournals.org/content/15/3/281.figures-only
*  Table of Contents | Molecular Cancer Research
The Long (lncRNA) and Short (miRNA) of It: TGFβ-Mediated Control of RNA-Binding Proteins and Noncoding RNAs ... Chromatin, Epigenetics and RNA Regulation. *. Chromatin, Epigenetics and RNA Regulation BRD1-Mediated Acetylation Promotes ... BET Proteins Exhibit Transcriptional and Functional Opposition in the Epithelial-to-Mesenchymal Transition ... BET Proteins Exhibit Transcriptional and Functional Opposition in the Epithelial-to-Mesenchymal Transition ...
http://mcr.aacrjournals.org/content/16/4?current-issue=y
*  Index by author | Molecular Cancer Research
The Long (lncRNA) and Short (miRNA) of It: TGFβ-Mediated Control of RNA-Binding Proteins and Noncoding RNAs ... The Long (lncRNA) and Short (miRNA) of It: TGFβ-Mediated Control of RNA-Binding Proteins and Noncoding RNAs ... The Long (lncRNA) and Short (miRNA) of It: TGFβ-Mediated Control of RNA-Binding Proteins and Noncoding RNAs ... The Long (lncRNA) and Short (miRNA) of It: TGFβ-Mediated Control of RNA-Binding Proteins and Noncoding RNAs ...
http://mcr.aacrjournals.org/content/16/4.index-by-author
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CDC27 expression is controlled posttranscriptionally through the RNA binding protein poly(rC) binding protein 1 (PCBP1)/ ... Implications: The RNA-binding protein HNRNP E1 mediates translational regulation of the cell-cycle regulator CDC27 and that ...
http://mcr.aacrjournals.org/content/early/2016/07/07/1541-7786.MCR-16-0018
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A novel RNA-binding protein, Ossa/C9orf10, regulates activity of Src kinases to protect cells from oxidative stress-induced ... They encode 3 closely related Ras proteins, H-Ras, K-Ras, and N-Ras, which are localized at the inner plasma membrane, bind GDP ... Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the cupin superfamily. J Biol ... The Src-associated protein CUB domain-containing protein-1 regulates adhesion and motility. Oncogene 2012;31:653-63. ...
http://mcr.aacrjournals.org/content/12/10/1449
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von Hippel-Lindau protein binds hyperphosphorylated large subunit of RNA polymerase II through a proline hydroxylation motif ... Identification of the RNA polymerase II subunit hsRPB7 as a novel target of the von Hippel-Lindau protein. EMBO J 2003;22:4249- ... Ubiquitination of hypoxia-inducible factor requires direct binding to the β-domain of the von Hippel-Lindau protein. Nat Cell ... The von Hippel-Lindau tumor suppressor protein is a component of an E3 ubiquitin-protein ligase activity. Genes Dev 1999; 13: ...
http://mcr.aacrjournals.org/content/2/2/89
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CDC27 expression is controlled posttranscriptionally through the RNA binding protein poly(rC) binding protein 1 (PCBP1)/ ... Implications: The RNA-binding protein HNRNP E1 mediates translational regulation of the cell-cycle regulator CDC27 and that ... In summary, we have shown that Cdc27 is translationally repressed by the RNA-binding protein HNRNP E1 in a cell cycle-dependent ... binding protein 1 (PCBP1)/heterogeneous nuclear ribonuclear protein E1 (HNRNP E1) and eukaryotic elongation factor 1A1 (EEF1A1 ...
http://mcr.aacrjournals.org/content/14/7/634
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Differential expression of S19 ribosomal protein, laminin-binding protein, and human lymphocyte antigen class I messenger RNAs ... Protein-bound beads were washed extensively with cold TNN buffer; proteins were eluted by boiling SDS protein loading buffer ... blocked rRPS2 binding to the 32P-ATP-labeled 31-nt RNA probe (Fig. 1B). rRPS2 was also found to bind pre-let-7a-1 RNA (Fig. 1C ... Determinants of microRNA processing inhibition by the developmentally regulated RNA-binding protein LIN28. J Biol Chem 2008;283 ...
http://mcr.aacrjournals.org/content/9/1/36
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Ichikawa H, Shimizu K, Hayashi Y, Ohki M. An RNA-binding protein gene, TLS/FUS, is fused to ERG in human myeloid leukemia with ... TLS-ERG leukemia fusion protein inhibits RNA splicing mediated by serine-arginine proteins. Mol Cell Biol 2000;20:3345-54. ... 4B). These results showed that transformation and deregulation of Cdk1 protein by TLS-ERG require a functional ets DNA-binding ... TLS-ERG contains an intact ets DNA-binding domain and is capable of binding to DNA in a sequence-specific manner (5). A point ...
http://mcr.aacrjournals.org/content/6/5/862
*  A Key Role for Cyclic AMP-Responsive Element Binding Protein in Hypoxia-Mediated Activation of the Angiogenesis Factor CCN1 ...
Total RNA was extracted using SV total RNA purification kit according to the manufacturer's instructions (Promega). RNA was ... is enhanced upon hypoxia stress primarily in a protein kinase A and cyclic AMP-responsive element binding protein (CREB) and ... with 1 μg of recombinant CREB protein or 10 μg of nuclear proteins. The protein/DNA complexes were subjected to gel ... A pivotal role of cyclic AMP-responsive element binding protein in tumor progression. Cancer Res 2004;64:1338-46. ...
http://mcr.aacrjournals.org/content/6/9/1397
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These short noncoding RNAs repress protein synthesis by binding to mRNA to inhibit translation or promote their degradation (9 ... RNA extraction and real-time RT-PCR. Total RNA was isolated from cell lines using the PureLink RNA Mini Kit (12183018A; Thermo ... A few RNA samples were cleaned using the Zymo RNA Clean and Concentrator Kit (R1015; Zymo Research). The mirVana miRNA ... Nuclear PARP-1 protein overexpression is associated with poor overall survival in early breast cancer. Ann Oncol 2012;23:1156- ...
http://mcr.aacrjournals.org/content/15/3/340
*  DJBP: A Novel DJ-1-Binding Protein, Negatively Regulates the Androgen Receptor by Recruiting Histone Deacetylase Complex, and...
RS, another name for human DJ-1, has been reported by another group as a protein of an RNA-binding protein regulatory subunit ( ... androgen receptor-interacting protein 3) as a DJ-1-binding protein (3). PIASxα/ARIP3 belongs to the PIAS family of proteins, ... We later identified PIAS (protein inhibitor of activated STAT)xα as a DJ-1-binding protein, and found that DJ-1 restored ... DJBP might also bind to other nuclear receptors. Of the proteins that bind to AR-DBD, SNURF (44), Ubc9 (41), and ARIP3/PIASXα ( ...
http://mcr.aacrjournals.org/content/1/4/247.long
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RNA-protein-binding reactions were washed with a buffer containing 100 mmol/L KCl, 20 mmol/L Tris-HCl at pH 7.5, and 0.2 mmol/L ... Indeed, we further showed that the RNA trans-factor, hnRNP L, bound this sequence in NSCLC cells to repress the inclusion of ... New streptavidin-agarose beads were then added to RNA-binding reactions and incubated at 4°C for 2 hours with gentle agitation ... After 48 hours, total RNA isolation or total protein lysates were collected as previously described in our laboratory (15) for ...
http://mcr.aacrjournals.org/content/9/7/889
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... binding protein (PABP) in specific regulation of YB-1 mRNA translation. RNA Biol 2011;8:883-92. ... Among other proteins tested, GATA4 markedly bound to its own promoter upon MYC expression. On the other hand, MAZ (16) and the ... The Y-box-binding protein 1 regulates the expression of many important genes (24) and is involved in the development of ... 5B). In A549 J5-1, MAZ and EZH2 binding was greatly reduced, whereas chk-MYC (the protein expressed in this cell line) and ...
http://mcr.aacrjournals.org/content/11/2/161
*  Genome Sequencing and RNA-motif Analysis Reveal Novel Damaging Non-coding Mutations in Human Tumors | Molecular Cancer Research
... affecting binding sites for RNA-binding proteins (RBPs) in cancer. Extracting signals related to RNA-related selection ... processes and using RNA sequencing (RNA-seq) data from the same specimens, we identified alterations in RNA expression and ... and long non-coding RNAs, that impact RNA processing and provide a new layer of insight that can aid in the interpretation of ... Genome Sequencing and RNA-motif Analysis Reveal Novel Damaging Non-coding Mutations in Human Tumors. Babita Singh, Juan L. ...
http://mcr.aacrjournals.org/content/early/2018/03/28/1541-7786.MCR-17-0601
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... include the heterogeneous nRNA binding proteins HnRNPU, Nucleolin, RMB15, and RMB25; the splicing factor U5S1; the RNA helicase ... Total RNA and protein were extracted using the Qiagen RNeasy Mini Kit. A, RNA was analyzed for the presence of Kap1 and ErbB4 ... but they may be linked to the presence of protein binding domains in the CYT-1-specific exon. These include a binding site for ... Bound protein was solubilized by heating at 100°C in 2× Laemmli sample buffer. Immunoblots on polyvinylidene difluoride ...
http://mcr.aacrjournals.org/content/8/10/1388
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Nuclear proteins (5 μg) were first incubated under binding conditions [10 mmol/L HEPES, Tris-HCl (pH 7.9), 50 mmol/L KCl, 0.1 ... and this was confirmed by PPARγ small interfering RNA (siRNA; Fig. 1F). Note that fish oil induced PPARγ protein expression ( ... Protein concentrations were determined by the Bio-Rad protein assay. Equal amounts of protein from whole-cell lysates were ... The roles of phosphatidylinositol 3-kinase and CCAAT/enhancer-binding protein β. J Biol Chem 2005;280:33240-9. ...
http://mcr.aacrjournals.org/content/7/1/108
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... and cleaned by preincubation with protein A-Sepharose beads to remove nonspecifically bound proteins. After precipitation with ... Conversely, knockdown of YWHAZ levels with siRNA or short hairpin RNA (shRNA) in invasive cancer cells led to a reversal of EMT ... YWHAZ binding to β-catenin prevents β-catenin from ubiquitin-mediated degradation. A, an increase of the β-catenin proteins in ... E, reduced binding activity of β-catenin with β-TrCP in presence of V5-tagged YWHAZ. The immunoprecipitated β-catenin proteins ...
http://mcr.aacrjournals.org/content/10/10/1319
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... including ATP-binding/protein kinase activity, pleckstrin homology (related to rho protein signal transduction and cytoskeleton ... RNA was prepared using a Qiagen RNeasy Plus RNA extraction kit. RNA was reverse transcribed and assessed for hypoxic induction ... For example, genes involved in ATP binding and protein kinase activity are not hypoxia induced, but exhibit AS. Increased exon ... CoAA, a nuclear receptor coactivator protein at the interface of transcriptional coactivation and RNA splicing. Mol Cell Biol ...
http://mcr.aacrjournals.org/content/12/9/1233
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Bromodomains are conserved protein folds that recognize and bind acetylated lysines. The four members of the BET family ( ... To interrogate the scope of BET protein regulation of AR output, we performed RNA profiling experiments. DHT stimulation of ... s effect on AR chromatin binding related to BRD4′s function as a multivalent scaffold protein that both engages and stabilizes ... the bromodomains of BET proteins can bind to acetyl-lysines on transcription factors, including NF-κB and TWIST to specify gene ...
http://mcr.aacrjournals.org/content/15/1/35
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Photoaffinity labels for estrogen binding proteins of rat uterus. Biochemistry 1973;12:4085-92. ... RNA was purified from these EGFP-positive cells and assayed by quantitative real-time PCR for amounts of pS2, PgR, ErbB2, and ... ERα and ERRα1 can both bind EREs, competing for binding to them (refs. 6-8; Fig. 3C). Vanacker et al. (7) reported that ERα can ... ERRα1 bound these sites with a variety of affinities (Table 1 ). Interestingly, ERRα1 bound the PgR site 1 with a higher ...
http://mcr.aacrjournals.org/content/5/1/71
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... activator protein 1, p53, and Rb/E2F pathways (13) and defective cyclic AMP-responsive element binding protein transcriptional ... Total RNA was isolated with Qiagen RNeasy kit (Qiagen Sciences). The quality of all RNA samples was confirmed using an Agilent ... Participation of ATM in insulin signalling through phosphorylation of eIF-4E-binding protein 1. Nat Cell Biol 2000;2:893-8. ... Grimberg A, Cohen P. Role of insulin-like growth factors and their binding proteins in growth control and carcinogenesis. J ...
http://mcr.aacrjournals.org/content/5/8/813
*  AMPK Causes Cell Cycle Arrest in LKB1-Deficient Cells via Activation of CAMKK2 | Molecular Cancer Research
The active form of the metabolic sensor: AMP-activated protein kinase (AMPK) directly binds the mitotic apparatus and travels ... ribosomal RNA and protein biosynthesis, and hence cell growth (1-3). Pharmacologic activation of AMPK also triggers a G1 cell ... Protein concentrations were determined by Coomassie Blue binding with BSA as standard (40). ... Calmodulin-dependent protein kinase kinase-beta is an alternative upstream kinase for AMP-activated protein kinase. Cell Metab ...
http://mcr.aacrjournals.org/content/14/8/683.full?ijkey=peoCc.9qdA3cg&keytype=ref&siteid=aacrjnls%3Futm_source%3D150479&utm_medium=landing3&utm_campaign=AMMR
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Cyclic (c)AMP responsive element binding protein (CREB) is a 43-kDa protein that belongs to the leucine-zipper class of ... The RNA was digested by incubation with RNase A (30 μg/mL) for 30 minutes in the dark at room temperature, followed by the ... Multisite phosphorylation of the cAMP response element-binding protein (CREB) by a diversity of protein kinases. Front Biosci ... However, a detailed analysis of the protein/DNA and protein/protein interactions of CREB is required, which might lead to the ...
http://mcr.aacrjournals.org/content/11/11/1462
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RNAs that regulate gene expression by interfering with the translation or stability of target transcripts through binding to ... of the p62 protein, a substrate for protein degradation by autophagy, may also stabilize NRF2 by competitively interacting with ... The bound antibodies were visualized with HRP staining solution or with an ECL Western detection kit according to the ... The bound antibody was visualized using diaminobenzidine as a chromogen (VECTASTAIN_ Elute ABC kit; Vector Laboratories), and ...
http://mcr.aacrjournals.org/content/12/1/58