... end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex. Kellner M, et al. Exp Cell Res, 2015 May 15. ... RRP12 ribosomal RNA processing 12 homolog [Homo sapiens] RRP12 ribosomal RNA processing 12 homolog [Homo sapiens]. Gene ID: ... ribosomal RNA processing 12 homologprovided by HGNC. Primary source. HGNC:HGNC:29100 See related. Ensembl:ENSG00000052749 MIM: ... RRP12 ribosomal RNA processing 12 homolog [ Homo sapiens (human) ] Gene ID: 23223, updated on 7-Sep-2023 ...

Post-transcriptional modifications of miRNAs with 3 non-templated nucleotide additions (NTA) are a common phenomenon, and for ... End Processing / genetics* * Real-Time Polymerase Chain Reaction Substances * MicroRNAs ... RNA 3 ... We previously showed that miRNA 3 additions are regulated by ... Here we examine the changes in abundance of miRNAs that exhibit altered 3 NTA following the suppression of a panel of ...

Process. Evidence Code. Pubs. involved_in box C/D RNA 3-end processing IBA Inferred from Biological aspect of Ancestor. more ... Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure youre on a federal ... Project title: HPA RNA-seq normal tissues. *Description: RNA-seq was performed of tissue samples from 95 human individuals ... particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 ...

... end RNA-Seq. processed data file: PS041-2_R2_0.txt. Data processing. Read 1 of our single cell RNA-Seq data contains a cell- ... polyA RNA. Extraction protocol. RNA was extracted from individual cells in individual microfluidic chambers following cell ... The processed data files contain the number of molecules counted for each gene based on counting reads with HTSeq and filtering ... Supplementary_files_format_and_content: The processed data files contain the number of molecules counted for each gene based on ...

End Maturation Module for Nuclear Non-coding RNAs. Son A, et al. Cell Rep, 2018 Apr 17. PMID 29669292 ... end processing. Located in Cajal body and cytoplasm. Implicated in pontocerebellar hypoplasia type 7. [provided by Alliance of ... Project title: HPA RNA-seq normal tissues. *Description: RNA-seq was performed of tissue samples from 95 human individuals ... end processing IBA Inferred from Biological aspect of Ancestor. more info ...

RNA Precursors / antagonists & inhibitors* * RNA Precursors / chemistry * Signal Transduction Substances * 3 Untranslated ... end elements involved in mRNA processing using antisense oligonucleotide drugs can be used as a strategy for gene silencing ... end elements involved in DUX4 mRNA processing is an efficient therapeutic strategy for facioscapulohumeral dystrophy: a new ... end elements involved in pre-mRNA to mRNA maturation with antisense drugs can lead to efficient gene silencing and is thus a ...

total RNA. Extraction protocol. Single-cell suspensions were processed through Drop-Seq to generate single-cell cDNA libraries ... end of the cDNA prepared for sequencing using a modified Nextera XT protocol.. ... Data processing. Base calls performed with bcl2fastq2.15. Read 2 was aligned with Star v2.4.0. Each aligned read was tagged ... Microbeads were counted, and amplified by PCR, and the 3 ... Processed data provided as supplementary file. Processed data ...

... end has been generally linked to RNA degradation. In mammals, uridylated pre-let-7 miRNAs and mRNAs are targeted by the 3 to 5 ... Uridylation of various cellular RNA species at the 3 ... of most of these identified RNAs are aberrant processing and ... We found a broad spectrum of uridylated RNAs including rRNAs, snRNAs, snoRNAs, tRNAs, vault, 7SL, Y RNAs, mRNAs, lncRNAs, and ... Most importantly, we demonstrate that uridylation mediates DIS3L2 degradation of short RNA polymerase II-derived RNAs. Our ...

Data processing. RNA-Seq: Reads were aligned to the mouse mm9 genome (NCBI Build 37) using STAR (v2.0.3e). RNA-Seq experiments ... gene end, 5: strand, 6: mRNA length, 7: Copies of gene in genome, 8: Annotation information, 9-18: RPKM gene expression values ... polyA RNA. Extraction protocol. RNA was purified using RNeasy Mini Kit (Qiagen) and enriched for Poly(A)-RNA with MicroPoly(A) ... fragmented with RNA Fragmentation Reagents (Ambion) and purified by a P-30 column (Biorad).. Fragmented RNA was ...

Data processing. RNA-Seq: Reads were aligned to the mouse mm9 genome (NCBI Build 37) using STAR (v2.0.3e). RNA-Seq experiments ... gene end, 5: strand, 6: mRNA length, 7: Copies of gene in genome, 8: Annotation information, 9-18: RPKM gene expression values ... polyA RNA. Extraction protocol. RNA was purified using RNeasy Mini Kit (Qiagen) and enriched for Poly(A)-RNA with MicroPoly(A) ... fragmented with RNA Fragmentation Reagents (Ambion) and purified by a P-30 column (Biorad).. Fragmented RNA was ...

12 samples, 150 bp single-ended RNA-Seq libraries from cell extracts after performing RTS reaction in different buffers (Li, K ... rG4s that have analogues in many ortholog genes across different species show a preferential association with RNA processing ... RNA structures are of crucial importance for biological function and gene regulation. Guanine (G)-rich sequences in RNA can ... Our data reveals that rG4s can be found in messenger RNAs (mRNAs) and long intergenic noncoding RNAs (lincRNAs). In mRNAs, rG4s ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

total RNA. Extraction protocol. Total RNA from bulk samples was extracted using Quick-RNA Mini Prep (Zymo Research) following ... end methods and read count matrices for Smart-seq2). More specifically, STAR v2.6.1a was used for alignment except for Smart- ... Data processing. For scRNA-seq and snRNA-seq data, scumi v0.1.0 (https://bitbucket.org/jerry00/scumi-dev/src/master/) was used ... For bulk RNA-Seq data, reads were mapped to the reference genomes using Bowtie v0.12.9, and further quantified by RSEM v1.3.0 ...

... and thawed at RT for 10 minutes prior to RNA purification using Ampure RNA beads (Beckmann-Coulter). RNA samples were re- ... Data processing. The bcl2fastq 2.16.0.10 software package was used to convert base call (BCL) files generated on the NextSeq ... end of the cDNA using a template-switching oligonucleotide (custom synthesized from Exiqon) as a template. Subsequently, cDNA ... total RNA. Extraction protocol. Whole embryos were dissected at the indicated timepoints and the correct stage was verified ...

... and thawed at RT for 10 minutes prior to RNA purification using Ampure RNA beads (Beckmann-Coulter). RNA samples were re- ... Data processing. The bcl2fastq 2.16.0.10 software package was used to convert base call (BCL) files generated on the NextSeq ... end of the cDNA using a template-switching oligonucleotide (custom synthesized from Exiqon) as a template. Subsequently, cDNA ... total RNA. Extraction protocol. Whole embryos were dissected at the indicated timepoints and the correct stage was verified ...

Data processing. Pair-end, 36-bp reads from each sample were aligned to the mouse genome (mm9 build) using TopHat (version 1.1. ... Quality of RNA was verified using bioanalyzer (Agilent); only RNA with a RIN , 9 was used for RNA-Seq. cDNA libraries were ... polyA RNA. Extraction protocol. For each insulin resistance-inducing condition and the control condition, RNA-Seq experiments ... 10ug of total RNA was used for each RNA-Seq library preparation according to the manufacturers instructions (Illumina). ...

The protocol was ended at D54.. Extracted molecule. polyA RNA. Extraction protocol. To generate single cell suspensions, hESC- ... Data processing. Reads were de-multiplexed by CelSeq index, allowing for one sequence mismatch.. The transcript reads for each ... RNA was fragmented using the NEBNext RNA Fragmentation Module (NEB), purified using an equal volume of RNA Clean Beads, and ... Single cell pools or population RNA libraries were purified with an equal volume of RNA Clean Beads (Beckman Coulter), linearly ...

total RNA. Extraction protocol. Illumina mRNA Library Preparation Protocol (Total RNA extracted from mammary tissue, Purify ... Data processing. Illumina software pipeline ( Basecalls was done by using Illumina RTA 1.6 for GAIIx run, RTA 1.7 for HiSeq run ... ends, Ligate the Adapters, Purify the cDNA Templates on a gel to select a size range). ... Gene expression of polyoma middle T antigen induced mammary tumors [RNA_Seq : MOLF x PyMT]. ...

... end adenylation of these products, Illumina Paired-End Sequencing adapters were ligated to the blunt ends of the cDNA fragments ... polyA RNA. Extraction protocol. Total RNA was extracted using the Trizol (Invitrogen) procedure or RNAeasy/RNAeasy Lipid/ ... This improvement was more pronounced for the sequencing runs processed with early versions of the Illumina pipeline, but ... Briefly, polyadenylated RNA was isolated using a poly-dT bead procedure and then chemically fragmented and randomly primed for ...