Combined Bisulfite Restriction Analysis - MyBioSource Learning Center
RNase A.. *Phenol/chloroform/isoamyl (25:24:1).. *Ethanol.. *TE Buffer: 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA. ...
Sanger Sequencing - MyBioSource Learning Center
RNase solution: 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, RNase A (10 mg/mL). Dissolve enzyme completely and keep the solution at 100 ... Add 1 mL of RNase solution and incubate for 15 min at room temperature. ...