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*  Refolding by disulfide isomerization - the mixed disulfide between ribonuclease t-1 and glutathione as a model refolding...
... was studied using the mixed disulfide between glutathione and reduced ribonuclease T-1 (GS-RNase T-1) as a stable soluble and ... appearance of native ribonuclease activity, HPLC, and nonreducing SDS-PAGE, All the analyses indicated that native RNase T-1 ... GS-RNase T-1 refolding was inhibited by BiP; refolding was completely blocked in presence of a 5-fold molar excess of BiP, and ... In 1.5 M NaCl, GS-RNase T-1 adopts a compact native-like conformation; refolding by thiol-disulfide interchange in these ...
https://kar.kent.ac.uk/19005/
*  New PTI QuantaMaster™ 8000 Series Fluorometers - HORIBA
However, the true potential of this technique can be seen in the case of Room Temperature Phosphorescence (RTP) of RNase T1 ... Discrimination between strong fluorescence and weak Room Temperature Phosphorescence (RTP) from RNase T1 tryptophan by varying ... Phosphorescence decay of a weakly emitting RNase T1 tryptophan signal using the same instrument.. ...
http://www.horiba.com/us/en/scientific/products/fluorescence-spectroscopy/steady-state/new-pti-quantamaster-8000-series-fluorometers/
*  Molecular Vision: XRCC1, but not APE1 and hOGG1 gene polymorphisms is a risk factor for pterygium
The aqueous supernatant was incubated with RNase A and RNase T1 (250 mg/ ml; Sigma Chemical Co., St. Louis, MO) at 37 °C for 60 ... Kerb R, Brockmoller J, Reum T, Roots I. Deficiency of glutathione S-transferases T1 and M1 as heritable factors of increased ...
http://www.molvis.org/molvis/v16/a109/
*  Protein Enzyme Kinetics - Olis, Inc.
2002). Kinetic studies of guanine recognition and a phosphate group subsite on ribonuclease T1 using substitution mutants at ... K Kumar and FG Walz, Jr.. (2001). Probing Functional Perfection in Substructures of Ribonuclease T1:? Double Combinatorial ...
http://olisweb.com/applications/protein-enzyme-kinetics/
*  Plus it
Louis, MO). Proteinase K, RNase A, and RNase T1 were from Boehringer Corp. (Lewes, Sussex, United Kingdom). Water-saturated ... followed by digestion with RNase A and T1. ...
http://cancerres.aacrjournals.org/content/59/19/4829
*  WikiGenes - Microdialysis
Crystal structure of ribonuclease T1 complexed with adenosine 2'-monophosphate at 1.8-A resolution. Ding, J., Koellner, G., ... Ribonuclease T1 was purified from an Escherichia coli overproducing strain and co-crystallized with adenosine 2'-monophosphate ...
http://www.wikigenes.org/e/mesh/e/13536.html
*  Patent US6153421 - Cloned genomes of infectious hepatitis C viruses and uses thereof - Google Patents
The cDNA mixture was treated with RNase H and RNase T1 (GIBCO BRL) at 37 C. for 20 minutes. The first round of PCR was ... The cDNA mixture was treated with RNase H and RNase T1 (GIBCO/BRL) for 20 min at 37 C. ...
http://www.google.com/patents/US6153421?dq=5179747
*  Cellular, molecular and developmental biology. - Free Online Library
Reagents used included chemicals [Pb.sup.2+], DMS, and DEPC and enzymes RNase T1 and nuclease SI. Different sizes of RNA ...
https://www.thefreelibrary.com/Cellular%2C+molecular+and+developmental+biology-a082474518
*  Roles of the influenza virus polymerase and nucleoprotein in forming a functional RNP structure | The EMBO Journal
Lanes 'C' are incubation controls without RNase. Lanes '1' are RNase T1 digestions of panhandle RNA under native conditions ... RNase T1 digestion. The digestion was carried out in buffer A (see above) with 0, 0.1, 0.5 or 1 U of the enzyme in a 300 μl ... The cleavage by RNase T1 is indicated by arrowheads. The scheme was drawn with the sequence of segment 8 vRNA in the centre. ... RNase T1 digestion of a 3′ end‐labelled, 81 nucleotide panhandle model RNA in the presence of increasing concentrations of DOC ...
http://emboj.embopress.org/content/16/6/1248
*  Plus it
RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis. Nucleic Acids Res ... In the same step, the in vitro transcription RNase A (SEQUENOM) was added to cleave the in vitro transcript. The mixture was ...
http://cancerres.aacrjournals.org/content/66/22/10911
*  Andrew Fire | Stanford Medicine Profiles
The RNA synthesized in vitro contains the same 5' capped RNase T1 undecanucleotide as does the in vivo transcript. RNA ... Incorporation was shown by label transfer experiments and by determining the size of 5'-terminal RNase T1-resistant ...
https://med.stanford.edu/profiles/andrew-fire
*  pancreatic ribonuclease - Creative Enzymes
ProductNameNative Aspergillus oryzae Ribonuclease T1. EC No.EC 3.1.27.3. CAS No.9026-12-4 ... RNase; RNase I; RNase A; pancreatic RNase; ribonuclease I; endoribonuclease I; ribonucleic phosphatase; alkaline ribonuclease; ... ribonuclease; gene S glycoproteins; Ceratitis capitata alkaline ribonuclease; SLSG glycoproteins; gene S locus-specific ... Ribonuclease can unwind the RNA helix by complexing with single-stranded RNA; the complex arises by an extended multi-site ...
https://www.creative-enzymes.com/product/Pancreatic-Ribonuclease_13772.html
*  Poly(A) tail length regulates PABPC1 expression to tune translation in the heart | eLife
RNase T1 treatment and poly(A) tail northern blots were performed as previously described10. After standardization the elution ... 1X RNase H buffer (Promega), 10 mM DTT, 15 ng/μL poly(A) (Sigma), 20 U RNasin (Promega), and 1 U RNase H. The reaction ... RNAse H digestion and northern blotting. To assess the Pabpc1 poly(A) tail lengths by northern blot, we mixed total RNA with ... RNAse free mortar and pestle. The powder obtained was transferred to a 10 cm plate, previously cooled on dry ice for 10 min. ...
https://elifesciences.org/articles/24139
*  An mRNA structure that controls gene expression by binding FMN | PNAS
Also, 5′ 32P-labeled ribD RNA precursors were not reacted (NR) or were subjected to partial digest with RNase T1 (T1) or alkali ... RNAs were subjected to no additional reaction (NR) or were subjected to partial digestion with RNase T1 (T1) or alkali (-OH) as ... The 3′ fragment of the run-off transcript (FL*) was subjected to partial digestion with RNase T1 or alkali as indicated. The ... Bands corresponding to the FL* and T* RNAs, along with several T1 digestion products, are identified. The T* RNA corresponds to ...
https://www.pnas.org/content/99/25/15908.full
*  Patent US7135312 - Circular DNA vectors for synthesis of RNA and DNA - Google Patents
RNase T1 sequencing of the monomeric products was performed by isolating the monomer bands produced from transcription of AS83 ... or with RNase T1 (according to the protocol supplied by USB). Nucleotide sequencing of the monomer-length RNAs confirmed them ... Alternatively, the enzyme RNase H can be used along with addition of a DNA oligomer, or base-specific RNases can be used. ... band about 150 nucleotides in length was isolated from an analogous transcription reaction and then treated with RNase T1. ...
http://www.google.com/patents/US7135312?dq=6246862
*  KCS Publications
Keywords: Hairpin RNA, RNA aptamer, RNase T1, Nuclease S1, RNase V1 DOI: https://doi.org/10.5012/bkcs.2008.29.10.2026 ...
http://journal.kcsnet.or.kr/main/j_search/j_archives_sub.htm?qpage=j_search&spage=b_bkcs&dpage=ar&year=2008&vol=29&no=10
*  US20040142095A1 - Nucleic acid arrays and method for detecting nucleic acids by using nucleic acid arrays - Google...
RNase T1 mediated base‐specific cleavage and MALDI‐TOF MS for high‐throughput comparative sequence analysis. ... Rnase inhibitor), 2 μl of 1 mM FluoroLink dUTP (Amersham Pharmacia, FluoroLink Cy5-dUTP) and 1 μl of SS II (LIFETEC ORIENTAL, ...
https://patents.google.com/patent/US20040142095
*  Transfer RNA methyltransferases with a SpoU-TrmD (SPOUT) fold and their modified nucleosides in tRNA
An early study indicated that Gm18 modification protects the d-loop region from RNase T1 digestion; therefore, it was ... In human mitochondria, a homolog of Trm10 (TRMT10C) is a subunit of RNase P and catalyzes the N1-methylation of purine at ... Vilardo E., Nachbagauer C., Buzet A., Taschner A., Holzmann J., Rossmanith W. A subcomplex of human mitochondrial RNase P is a ... Sen A., Karasik A., Shanmuganathan A., Mirkovic E., Koutmos A., Cox R.T. Loss of the mitochondrial protein-only ribonuclease P ...
http://pubmedcentralcanada.ca/pmcc/articles/PMC5372735/
*  KCS Publications
Keywords: G-rich RNA, RNA aptamer, RNase T1, Nuclease S1, RNase V1 Online date: September 23, 2011 DOI: https://doi.org/10.5012 ...
http://journal.kcsnet.or.kr/main/j_search/j_archives_sub.htm?qpage=j_search&spage=b_bkcs&dpage=ar&year=2011&vol=32&no=10
*  ASMscience | Modulatory Role of Modif
These fragments were obtained after purification of the RNase T1 hydrolysate of the corresponding tRNAs. Association constants ... These fragments were obtained after purification of the RNase T1 hydrolysate of the corresponding tRNAs. Association constants ... Processing of the Bacillus subtilis thrS leader mRNA is RNase-dependent in E. coli. J. Mol. Biol. 268:235-242.. ...
http://www.asmscience.org/content/book/10.1128/9781555818296.chap7
*  Plus it
RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis. Nucleic Acids Res ... Schatz P, Dietrich D, Schuster M. Rapid analysis of CpG methylation patterns using RNase T1 cleavage and MALDI-TOF. Nucleic ... In vitro Transcription and RNase A Cleavage. In the first step, transcription/RNase A cocktail for T-cleavage reaction was ... Following the in vitro transcription, RNase was added to cleave the in vitro transcripts. In the cleavage reaction, the reverse ...
http://mcr.aacrjournals.org/content/6/11/1702
*  Table of Contents | Journal of Virology
Analysis of vaccinia virus transcriptional complexity in vitro and in vivo: characterization of RNase T1-resistant 5'-terminal ...
https://jvi.asm.org/content/42/2
*  Dendritic BC1 RNA in translational control mechanisms | JCB
... followed by RNase T1 digestion (Muddashetty et al., 2002). Omission of heparin/T1 resulted in a weak shift of BC1 RNA by PABP ( ... When indicated, heparin was used at 5 mg/ml, and optional T1 RNase digestion was performed as described previously (Muddashetty ...
http://jcb.rupress.org/content/171/5/811.full
*  SB2010 - Rizzo Lab
Ribonuclease T1 7 OMP Decarboxylase 7 Estrogen Receptor 45 T4 Lysozyme 13 ...
http://ringo.ams.stonybrook.edu/index.php?title=SB2010&direction=prev&oldid=11308