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*  Patent US7135312 - Circular DNA vectors for synthesis of RNA and DNA - Google Patents
RNase T1 sequencing of the monomeric products was performed by isolating the monomer bands produced from transcription of AS83 ... or with RNase T1 (according to the protocol supplied by USB). Nucleotide sequencing of the monomer-length RNAs confirmed them ... Alternatively, the enzyme RNase H can be used along with addition of a DNA oligomer, or base-specific RNases can be used. ... band about 150 nucleotides in length was isolated from an analogous transcription reaction and then treated with RNase T1. ...
http://www.google.com/patents/US7135312?dq=6246862
*  Ribonuclease T1 - Wikipedia
... ribonuclease PP1, ribonuclease SA, RNase F1, ribonuclease C2, binase, RNase Sa, guanyl-specific RNase, RNase G, RNase T1, ... Aspergillus oryzae ribonuclease, RNase N1, RNase N2, ribonuclease N3, ribonuclease U1, ribonuclease F1, ribonuclease Ch, ... Similar to other ribonucleases such as barnase and RNase A, ribonuclease T1 has been popular for folding studies. Structurally ... RNase T1 also has four prolines, two of which (Pro39 and Pro55) have cis isomers of their X-Pro peptide bonds. Nonnative ...
https://en.wikipedia.org/wiki/Ribonuclease_T1
*  Guanine-specific ribonuclease N1/T1/U2 (IPR000026) | InterPro | EMBL-EBI
Guanine-specific ribonuclease N1/T1/U2 (IPR000026). Short name: Gua-sp_ribonuclease_N1/T1/U2 ... RNase N1), a guanine-specific ribonuclease from fungi [PMID: 2977130], and its related RNases from bacteria, RNase T1 and Sa. ... The amino acid sequence of ribonuclease N1, a guanine-specific ribonuclease from the fungus Neurospora crassa.. J. Biochem. 104 ... Subsite binding in an RNase: structure of a barnase-tetranucleotide complex at 1.76-A resolution.. Biochemistry 33 1644-53 1994 ...
http://www.ebi.ac.uk/interpro/entry/IPR000026
*  Refolding by disulfide isomerization - the mixed disulfide between ribonuclease t-1 and glutathione as a model refolding...
... was studied using the mixed disulfide between glutathione and reduced ribonuclease T-1 (GS-RNase T-1) as a stable soluble and ... appearance of native ribonuclease activity, HPLC, and nonreducing SDS-PAGE, All the analyses indicated that native RNase T-1 ... GS-RNase T-1 refolding was inhibited by BiP; refolding was completely blocked in presence of a 5-fold molar excess of BiP, and ... In 1.5 M NaCl, GS-RNase T-1 adopts a compact native-like conformation; refolding by thiol-disulfide interchange in these ...
https://kar.kent.ac.uk/19005/
*  RT-PCR Kits
Invitrogen™ Ambion™ RNase T1 (1U/μL) includes RNase T1, 10X RNA Structure Buffer, 1X Alkaline Hydrolysis Buffer, Yeast RNA, ... Invitrogen™ RNase T1 (1U/μL) Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and ... Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor Delivers extremely high-quality, single- ... High-purity enzyme that's a mix of cloned RNA polymerase and RNase inhibitor ...
https://www.fishersci.com/us/en/products/life-sciences/pcr-and-qpcr/pcr-reagents-andkits/rt-pcr-kits.html
*  Embryonic Lethal Abnormal Vision-Like RNA-Binding Proteins Regulate Neurite Outgrowth and Tau Expression in PC12 Cells |...
RNase T1 selection analysis of the HuD binding region in the tau-H fragment. RNase T1 selection assay was performed as ... The pattern of digestion of tau-H RNA with RNase T1 is shown in Figure 10 (lane 3). The tau-H RNA contains two large RNase T1 ... RNase T1 selection assay. Reaction mixtures (20 μl; see preceding section for contents) were incubated for 10 min at 37°C. ... To localize the HuD-binding region within the H segment of tau mRNA, we used the RNase T1 selection analysis that we have used ...
http://www.jneurosci.org/content/19/16/6907?ijkey=1d6bf7f9c6a606582c2dc087d332877f1d2f34e2&keytype2=tf_ipsecsha
*  Ribonucleases (RNases) | Thermo Fisher Scientific - SA
A table detailing the properties of Ribonucleases (RNases), Nucleases that catalyze the degradation of RNA, used to remove RNA ... Combines the activities of RNase A and RNase T1. ssRNA. *3'-CMP ... RNase A/T1 Mix. *Removal of RNA from DNA solutions *Removal of ... Ribonuclease. Applications. Reaction catalyzed. Substrate. Reaction products. RNase A, DNase and protease-free. *Removal of RNA ... Ribonuclease protection assays. Cleavage of phosphodiester bonds between 3'-C or 3'-U residues and the 5'-OH residue of an ...
https://www.thermofisher.com/sa/en/home/brands/thermo-scientific/molecular-biology/thermo-scientific-restriction-modifying-enzymes/modifying-enzymes-thermo-scientific/ribonucleases-rnases.html
*  Photoswitching of Site-Selective RNA Scission by Sequential Incorporation of Azobenzene and Acridine Residues in a DNA Oligomer
Lane 1, control in a buffer solution; lane 2, treatment with Lu(III) alone; lane 3, RNase T1 digestion; lane 4, 2 and Lu(III) ... Lane 1, control reaction in a buffer solution; lane 2, treatment with Lu(III) alone; lane 3, RNase T1 digestion; lane 4, 2 and ... T. Niittymaki and H. Lonnberg, "Artificial ribonucleases," Organic and Biomolecular Chemistry, vol. 4, no. 1, pp. 15-25, 2006. ... A. Kuzuya and M. Komiyama, "Site-selective artificial ribonucleases and their applications," Current Organic Chemistry, vol. 11 ...
https://www.hindawi.com/journals/jna/2011/162452/
*  pgl-1 - Guanyl-specific ribonuclease pgl-1 - Caenorhabditis elegans - pgl-1 gene & protein
ribonuclease T1 activity Source: UniProtKB-EC. *RNA binding Source: WormBase ,p>Inferred from Sequence or Structural Similarity ... Guanyl-specific ribonuclease pgl-1Curated (EC:3.1.27.31 Publication. Manual assertion based on experiment ini ... "PGL germ granule assembly protein is a base-specific, single-stranded RNase.". Aoki S.T., Kershner A.M., Bingman C.A., Wickens ... "PGL germ granule assembly protein is a base-specific, single-stranded RNase.". Aoki S.T., Kershner A.M., Bingman C.A., Wickens ...
https://www.uniprot.org/uniprot/Q9TZQ3
*  Bioinformatic analysis of riboswitch structures uncovers variant classes with altered ligand specificity | PNAS
T1, and −OH indicate no reaction, partial digestion with RNase T1 (cleaves after G residues), and partial digestion with ... Several RNase T1 product bands are labeled. Regions undergoing structural modulation (1 through 5) and predicted stems (P1 ...
http://www.pnas.org/content/114/11/E2077
*  Discovering ligands for a microRNA precursor with peptoid microarrays. - PubMed - NCBI
OH and T1 are alkaline hydrolysis ladder and ribonuclease T1 digestion, respectively. (B) Cleavage patterns in the presence and ...
https://www.ncbi.nlm.nih.gov/pubmed/19561197
*  3D Triple-resonance NMR techniques for the sequential assignment of NH and 15N resonances in 15N- and 13C-labelled proteins |...
3D NMR J connectivity Sequential assignment Isotope labelling Proteins Ribonuclease T1 This is a preview of subscription ... 1990) [Biochemistry, 29, 4659-4667]. Both techniques were applied to uniformly 15N- and 13C-labelled ribonuclease T1. ...
https://link.springer.com/article/10.1007%2FBF00242479
*  Laboratory Methods in Enzymology: Protein Part B, Volume 539 - 1st Edition
8 Step 4 Immunoprecipitation and Second RNase T1 Treatment (Day 2). 9 Step 5 Dephosphorylation and Radiolabeling RNA Segments ...
https://www.elsevier.com/books/laboratory-methods-in-enzymology-protein-part-b/lorsch/978-0-12-420120-0
*  Structures of Active Guide Rna Molecules and Method of Selection - Patzel, Volker
... and 0.001 U/μl Rnase T1 (Ambion, Austin, USA) respectively. Rnase T1 specifically cleaves single stranded RNA after guanosine ... 6: RNAse T1 probing of guide-siRNA structures 4-7, 0-0, and 2-9. Guide-siRNA strands were 5′-labeled with 32P using ... Vickers, T. A. Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents. A ... 5′-CGGCCAUGAUAUAGACGUUGU-3′, G-5-6-T1: sense: 5′-CGGCAUCAAGGUGAACUUCdTdT-3′, antisense: 5′-GAAGUUCACCUUGAUGCCGUU-3′; G-5-6-T2: ...
http://www.freepatentsonline.com/y2008/0280848.html
*  Deciphering Nuclease Digestion Data | Thermo Fisher Scientific - SA
RNase A, RNase V1 and RNase T1. These nucleases are ideal for RNA structure determination, sequencing, and protein footprinting ... To facilitate RNA structural studies, Ambion now offers 3 RNA-grade ribonucleases: ... To facilitate RNA structural studies, Ambion now offers 3 RNA-grade ribonucleases: RNase A, RNase V1 and RNase T1. These ... A schematic of the RNase T1 "results" can be seen in Figure 2, Panel B. RNase A, which cleaves single-stranded RNA at C and U ...
https://www.thermofisher.com/sa/en/home/references/ambion-tech-support/rna-structure-function/tech-notes/deciphering-nuclease-digestion-data.html
*  US20100279283A1 - Method for molecular biology applications - Google Patents
Ribonuclease T1: (Aspergillus) * *An endonuclease that cleaves RNA at 3′ phosphates of guanine residues, producing ... Ribonuclease H: (E. coli) * *The Ribonuclease H(RNase H) specifically degrades only the RNA strand in RNA-DNA hybrids. It does ... RNase H activity: RNase H is a ribonuclease that degrades the RNA from RNA-DNA hybrids, such as are formed during reverse ... Ribonuclease I: (RNase I) * *an endoribonuclease, preferentially hydrolyzes single-stranded RNA to nucleoside 3′-monophosphates ...
https://patents.google.com/patent/US20100279283
*  How can biochemical reactions within cells differ from those in test tubes? | Journal of Cell Science
Shastry, M. and Eftink, M. (1996). Reversible thermal unfolding of ribonuclease T1 in reverse micelles. Biochemistry 35, 4094- ...
http://jcs.biologists.org/content/119/14/2863
*  RNA gel shift - Molecular Biology
From most literature, people would like to add RNase T1 and heparin sulfate in the binding reaction. RNase T1 is used to digest ...
http://www.protocol-online.org/biology-forums/posts/19912.html
*  Chemistry & Biochemistry | Honors College | Kent State University
1978). Studies on the Absolute Base Group Specificity of Ribonuclease T1. Advisor: Frederick Walz. ... uridine for Use in the Study of Ribonuclease t1. Advisor: Frederick Walz ...
https://www.kent.edu/honors/senior-honors-thesis-archive/chemistry-biochemistry
*  Dysregulation of Iron Homeostasis in the CNS Contributes to Disease Progression in a Mouse Model of Amyotrophic Lateral...
Unbound probe was degraded by incubation for 10 min with RNase T1. The formation of RNA-protein complexes was then detected by ...
http://www.jneurosci.org/content/29/3/610.long
*  Chemical Approaches for Structure and Function of RNA in Postgenomic Era
One set is the catalog of T1 oligonucleotides produced by RNase T1. The other is the catalog of oligonucleotides produced by ... The mechanism catalyzed by alkaline hydrolysis, RNase A, T1 RNase, T2 RNase and U2 RNase involves a S. N. 2. (. p. ). mechanism ... The 5′ oligonucleotides from U1 RNA, obtained by RNase A and RNase T1, were digested with snake venom phosphodiesterase and ... The [3H]-labeled U3 RNA was digested with RNase A and or T1 RNase. The [3H]-labeled 5′ oligonucleotide obtained by RNase A ...
https://www.hindawi.com/journals/jna/2012/369058/
*  Protein Folding in the Endoplasmic Reticulum | SpringerLink
Conformational stability and activity of ribonuclease T1 with zero, one, and two intact disulfide bonds. J Biol Chem 1988; 263 ...
https://link.springer.com/chapter/10.1007%2F978-1-4684-9418-1_10
*  Mechanism for gene control by a natural allosteric group I ribozyme. - PubMed - NCBI
Lanes are labeled as follows: no reaction (NR), partial digest with RNase T1 (T1; cleaves after every G residue), partial ... Arrowheads indicate the full-length (FL) RNA band and other bands representing RNase T1 cleavage after selected G residues. ...
https://www.ncbi.nlm.nih.gov/pubmed/21960486
*  Analyses of genome architecture and gene expression reveal novel candidate virulence factors in the secretome of Phytophthora...
Kiefhaber T, Quaas R, Hahn U, Schmid FX: Folding of ribonuclease T1. 1. Existence of multiple unfolded states created by ... Deshpande RA, Shankar V: Ribonucleases from T2 family. Crit Rev Microbiol. 2002, 28: 79-122. 10.1080/1040-840291046704.PubMed ... RNA cleavage by ribonuclease T2 was shown to be implicated in defense and self-incompatibility processes [47]. Some of these ... The ribonuclease T2 (PF00445) and SpoU rRNA methylase (PF00588) are Pfam domains also enriched in the secretome (Figure 1C, ...
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-11-637
*  Nova autoregulation reveals dual functions in neuronal splicing | The EMBO Journal
RNase T1 digestion (T1) was used to generate size standards. Boundaries are highlighted by arrows and indicated on the ... RNase protection assays and RT-PCR. RNA preparation and RNase protection assays were performed as described previously (Dredge ... To confirm these results, we also performed RNase protection assays (RPA), which revealed a 24.6% decrease in steady‐state Nova ... The pellet was treated with RNase prior to separation by SDS-PAGE, transferred to nitrocellulose and exposed to autoradiography ...
http://emboj.embopress.org/content/24/8/1608