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RNase T1 mediated base-specific cleavage and MALDI-TOF MS for high-throughput comparative sequence analysis. Nucleic Acids Res ... Schatz P, Dietrich D, Schuster M. Rapid analysis of CpG methylation patterns using RNase T1 cleavage and MALDI-TOF. Nucleic ... In vitro Transcription and RNase A Cleavage. In the first step, transcription/RNase A cocktail for T-cleavage reaction was ... Following the in vitro transcription, RNase was added to cleave the in vitro transcripts. In the cleavage reaction, the reverse ...
http://mcr.aacrjournals.org/content/6/11/1702
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To generate cDNA, samples were treated with reverse transcription master mix (0.05 μL RNase inhibitor, 0.07 μL T4 gene 32 ... and then incubated with Dynabeads MyOne Streptavidin T1 (Invitrogen) to achieve magnetic labeling and depletion of white blood ... 0.3 μL RNase H, and 4.26 μL H2O per 1× volume) at 37°C for 15 minutes and inactivated at 70°C for 10 minutes. A second-strand ... Rnase Inhibitor, 0.5 μmol/L UP1 Primer, 10 mmol/L dNTP, and Nuclease-free water) and immediately flash frozen in liquid ...
http://mcr.aacrjournals.org/content/16/4/720
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From the original series, only N0 patients (n = 128) and among these, only those with T1/T2 tumors (n = 112), were included in ... The RNA was digested by incubation with RNase A (30 μg/mL) for 30 minutes in the dark at room temperature, followed by the ...
http://mcr.aacrjournals.org/content/11/11/1462