Trans-Silencing by P Elements Inserted in Subtelomeric Heterochromatin Involves the Drosophila Polycomb Group Gene, Enhancer of...
B) RNase protection analysis of total ovary RNA from P(1A)/FM6;+/TM3 (lane 1), P(1A)/FM6;E(z)/TM3 (lanes 2-4) and w1118 (lane 5 ... RNA samples were digested with RNases A and T1 for 1 hr at 15° instead of 1 hr at room temperature. The IVS3 probe plasmid, ... RNA isolation and RNase protection analysis: Ovaries were dissected from females fattened at 29° for 3 days and total ovary RNA ... To analyze expression of the Lk-P(1A) P elements, RNase protection experiments were performed using total ovary RNA isolated ...
Isolation and Characterization of HRT1 Using a Genetic Screen for Mutants Unable to Degrade Gic2p in Saccharomyces cerevisiae |...
We found that Hrt1p is unstable (t1/2 ~ 30 min), suggesting that the turnover of Hrt1p may be regulated by ubiquitin-dependent ... and digested with RNase A for 5 hr. The DNA was stained with propidium iodide and analyzed on a FACScan (Becton-Dickinson). ...