T1-1x, tumor - one round of amplification; T1-2x, tumor - two rounds of amplification; T2-1x, duplicate tumor - one round of ... Air-dried sections were lightly stained with RNase free heamatoxylin (20s), rinsed with DEPC-H2O, dehydrated with 100% ethanol ... A, T1, ratio 28S/18S = 1.9; B, T2, ratio 28S/18S = 1.9; C, B, ratio 28S/18S = 1.8. ... Bland and Altman plots show a large variability between one (1x) and two rounds (2x) of amplification for (A) T1 and (B) T2 ...

The two cell lines were subjected to four different treatments (treatments T1, T2, T3 and T4). After RNA isolation and labeling ... RNAse-free ethanol. Samples were then applied to an RNeasy Mini column (Qiagen, Valencia, California), and processed according ... For example, in dataset C1, the directly measured ratios between samples T1 and T2 on array 1, were compared with the ... T1, T2, T3 and T4) were investigated using two keratinocyte-derived cell lines by measuring transcript levels on Agilent 4 × ...

Recombinant proteins for CLK4, PK2, and actin I were expressed as fusion proteins with a GST-tag using the pGEX 4 T-1 vector ( ... RNA preparations were treated with RNAse-free DNAse I (Qiagen) to remove gDNA contamination, followed by phenol/chloroform ...

... which encodes a 3,947 aa protein with putative hits to viral related proteins including RNase H, integrases, retroposons, ... Eco RV cloned into T1 phage-resistant DH10B E. coli). ...

Principal component analysis (PCA). Size of the circles indicates time after exposure to the stress, i.e. 15 (T1), 30 (T2), 60 ... and contaminating genomic DNAs were removed with an RNase-Free DNase set (Qiagen, Hilden, Germany) according to the ...