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*  Patente US5538871 - In situ polymerase chain reaction - Google Patentes
... can be achieved by changing the way that the enzymatic reaction is started. Reaction initiation is delayed until the start of ... or by adding to the PCR reagents a single-stranded DNA binding protein which blocks reaction at temperatures below about 50 C. ... Improvements to the in situ polymerase chain reaction (PCR), a process of in vitro enzymatic amplification of specific nucleic ... Detection of allele-specific mutations by allele-specific in situ reverse transcriptase polymerase chain reaction. ...
http://www.google.es/patents/US5538871
*  Reverse transcriptase inhibitor legal definition of reverse transcriptase inhibitor
What is reverse transcriptase inhibitor? Meaning of reverse transcriptase inhibitor as a legal term. What does reverse ... Definition of reverse transcriptase inhibitor in the Legal Dictionary - by Free online English dictionary and encyclopedia. ... Reverse Transcription and Polymerase Chain Reaction Analysis. *Reverse Transcription Intron Differential Polymerase Chain ... Reverse Transcriptase Polymerase Chain Reaction. *Reverse Transcriptase Polymerase Chain Reaction. *Reverse Transcriptase ...
https://legal-dictionary.thefreedictionary.com/reverse+transcriptase+inhibitor
*  Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. |...
Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction.. R ... Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. ... Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. ... Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. ...
https://jcm.asm.org/content/30/3/545?ijkey=9058315d38910852fc7d6ccd4d3328f009d5332a&keytype2=tf_ipsecsha
*  Reverse Transcriptase-Polymerase Chain Reaction Technique - How is Reverse Transcriptase-Polymerase Chain Reaction Technique...
It is Reverse Transcriptase-Polymerase Chain Reaction Technique. Reverse Transcriptase-Polymerase Chain Reaction Technique ... Reverse Transcriptase-Polymerase Chain Reaction Technique. Looking for abbreviations of RT-PCR? ... Reverse Transcriptase-Polymerase Chain Reaction Technique - How is Reverse Transcriptase-Polymerase Chain Reaction Technique ... Reverse Transcriptase-Polymerase Chain Reaction Technique. RT-PCR. Reverse Transcription and Polymerase Chain Reaction Analysis ...
https://acronyms.thefreedictionary.com/Reverse+Transcriptase-Polymerase+Chain+Reaction+Technique
*  Expression Profiling of the Whole Arabidopsis Shaggy-Like Kinase Multigene Family by Real-Time Reverse Transcriptase-Polymerase...
... of the Whole Arabidopsis Shaggy-Like Kinase Multigene Family by Real-Time Reverse Transcriptase-Polymerase Chain Reaction. ... 2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol ... of the family has been performed using the technique of real-time quantitative reverse transcriptase-polymerase chain reaction ... of the Whole Arabidopsis Shaggy-Like Kinase Multigene Family by Real-Time Reverse Transcriptase-Polymerase Chain Reaction ...
http://www.plantphysiol.org/content/130/2/577.full
*  RePub, Erasmus University Repository: Multimarker Reverse Transcriptase-Polymerase Chain Reaction Assay in Lymphatic Drainage...
Multimarker Reverse Transcriptase-Polymerase Chain Reaction Assay in Lymphatic Drainage and Sentinel Node Tumor Burden. ... multimarker reverse transcriptase-polymerase chain reaction [MM-RT-PCR] with primers for tyrosinase, MART1 (MelanA), and uMAGE ... Multimarker Reverse Transcriptase-Polymerase Chain Reaction Assay in Lymphatic Drainage and Sentinel Node Tumor Burden. Annals ...
https://repub.eur.nl/pub/20595
*  Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus...
Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus ... aim of this study was to evaluate sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction ( ...
https://www.physiciansweekly.com/sensitivity-and-specificity-of-a-real-time-reverse-transcriptase-polymerase-chain-reaction-detecting-feline-coronavirus-mutations-in-effusion-and-serumplasma-of-cats-to-diagnose-feline-infectious-per/
*  Reverse Transcriptase Polymerase Chain Reaction | Anatomical Neuropharmacology Unit
This is an historical archive of the activities of the MRC Anatomical Neuropharmacology Unit (MRC ANU) that operated at the University of Oxford from 1985 until March 2015. The MRC ANU established a reputation for world-leading research on the brain, for training new generations of scientists, and for engaging the general public in neuroscience. The successes of the MRC ANU are now built upon at the MRC Brain Network Dynamics Unit at the University of Oxford.. ...
http://mrcanu.pharm.ox.ac.uk/research-and-techniques/reverse-transcriptase-polymerase-chain-reaction
*  Detection of human 11β-hydroxysteroid dehydrogenase isoforms using reverse-transcriptase-polymerase chain reaction and...
Using the reverse-transcriptase polymerase chain reaction (RT-PCR) amplification of RNA extracted from human adult tissues, ... Detection of human 11β-hydroxysteroid dehydrogenase isoforms using reverse-transcriptase-polymerase chain reaction and ... Detection of human 11β-hydroxysteroid dehydrogenase isoforms using reverse-transcriptase-polymerase chain reaction and ...
https://www.semanticscholar.org/paper/Detection-of-human-11%CE%B2-hydroxysteroid-dehydrogenase-Whorwood-Mason/22139c1adb7f5a59db38293316bae6bb8a757054
*  Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and...
Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used in rabies diagnosis with good results, even in ... Reverse-transcriptase Polymerase Chain reaction (RT-PCR) and Heminested RT-PCR (hnRT-PCR) results for detection of rabies virus ... Heminested reverse-transcriptase polymerase chain reaction (hnRT-PCR) as a tool for rabies virus detection in stored and ... Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used in rabies diagnosis with good results, even in ...
https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-1-17
*  Confirmation of Rabies Infection by Mouse Inoculation Test and Reverse Transcriptase-Polymerase Chain Reaction in Suspected...
Confirmation of Rabies Infection by Mouse Inoculation Test and Reverse Transcriptase-Polymerase Chain Reaction in Suspected ... Confirmation of Rabies Infection by Mouse Inoculation Test and Reverse Transcriptase-Polymerase Chain Reaction in Suspected ... Confirmation of Rabies Infection by Mouse Inoculation Test and Reverse Transcriptase-Polymerase Chain Reaction in Suspected ... Reverse transcriptase-polymerase chain reaction (RT-PCR) performed for both samples gave a product of 443-bp amplifying the ...
http://researcherslinks.com/current-issues/Confirmation-of-Rabies-Infection-by-Mouse/20/3/1772/html
*  Acute promyelocytic leukemia: the study of t(15;17) translocation by fluorescent in situ hybridization, reverse transcriptase...
... reverse transcriptase-polymerase chain reaction and cytogenetic techniques. Autor Chauffaille, Maria de Lourdes Lopes Ferrari ... reverse transcriptase-polymerase chain reaction (RT-PCR) and karyotyping in 18 samples (12 at diagnosis and 6 after treatment) ... reverse transcriptase-polymerase chain reaction and cytogenetic techniques. Entrar ...
http://repositorio.unifesp.br/handle/11600/1166
*  Prolonged measles virus shedding in human immunodeficiency virus-infected children, detected by reverse transcriptase-...
A reverse transcriptase-polymerase chain reaction assay was used to detect measles virus RNA in peripheral blood mononuclear ... Reverse Transcriptase Polymerase Chain Reaction, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S., Time Factors, Urine, ... detected by reverse transcriptase- polymerase chain reaction. The Journal of infectious diseases, 183 (4). pp. 532-8. ISSN 0022 ... detected by reverse transcriptase- polymerase chain reaction ...
http://researchonline.lshtm.ac.uk/16909/
*  Augmenter of Liver Regeneration (ALR) Is a Novel Biomarker of Hepatocellular Stress/Inflammation: In Vitro, In Vivo and In...
Reverse Transcriptase-Polymerase Chain Reaction. Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was ... reverse) (681 bp); and β-actin, TTCTA CAATG AGCTG CGTGT G (forward) and TTCAT GGATG CCACA GGATT C (reverse) (561 bp). The PCR ... Yang H, et al. (2004) Reversing established sepsis with antagonists of endogenous high-mobility group box 1. Proc. Natl. Acad. ... and the NO reaction products NO2−/NO3− (D) were measured as described in Materials and Methods. *p , 0.05 versus 0 time. ...
https://link.springer.com/article/10.2119%2Fmolmed.2012.00183
*  Plus it
Semiquantitative reverse transcriptase polymerase chain reaction. Oligonucleotide primers (Tyndale et al., 1994) for α4 (503 bp ... Assessment of α4 mRNA levels after 3α,5α-THP withdrawal with semiquantitative reverse transcriptase polymerase chain reaction ( ... were housed in groups of three under a reverse light/dark cycle (14/10 hr light/dark). Food and water were available ad libitum ... Total RNA was isolated using Tri reagent and a chloroform extraction and was followed by reverse transcription (SuperScript II ...
http://www.jneurosci.org/content/18/14/5275
*  Balance of MafB and PU.1 specifies alternative macrophage or dendritic cell fate | Blood Journal
Reverse transcriptase-polymerase chain reaction. Total RNA was extracted from cells using RNeasy Mini Kit (Qiagen, Hilden, ... PCR amplifications were performed using 2 μL of the reverse transcription products in 25 μL of reaction with puReTaq Ready-to- ... G) Mixed lymphocyte reaction (MLR) of myeloid cells differentiated by PU.1 or MafB activation. Ctrl, PU.1, and MafB clones were ... Pierre P, Mellman I. Developmental regulation of invariant chain proteolysis controls MHC class II trafficking in mouse ...
http://www.bloodjournal.org/content/105/7/2707?ijkey=18f06efd57d6b5be88cf4c54c173d05b240c0121&keytype2=tf_ipsecsha&sso-checked=true
*  Regulation of 6-Pyruvoyltetrahydropterin Synthase Activity and Messenger RNA Abundance in Human Vascular Endothelial Cells |...
Reverse Transcriptase-Polymerase Chain Reaction. The relative expression of GTPCH, PTPS, cNOS, and iNOS mRNAs compared with the ... Reverse-transcription/limiting-dilution polymerase chain reaction analysis showed that in response to IFN/TNF/IL-1, mRNA ... and first-strand cDNA was used as a template in polymerase chain reaction (PCR). cDNA aliquots were amplified with the ... For comparative determination of mRNA levels, limiting-dilution PCRs of cDNA were performed.13 14 After reverse transcription ( ...
http://circ.ahajournals.org/content/98/17/1703
*  Heat-Shock Protein 60-Reactive CD4+CD28null T Cells in Patients With Acute Coronary Syndromes | Circulation
Reverse-Transcriptase Polymerase Chain Reaction. Cellular RNA was extracted with Trizol reagent and 20 μg glycogen followed by ... The CD4+CD28null phenotype was further confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR) analysis of mRNA ... Detection of cell specific cluster determinant expression by reverse transcriptase polymerase chain reaction. J Immunol Methods ... cDNA synthesis with Superscript II reverse transcriptase and random hexamers (Life Technologies). Amplification of IFN-γ and ...
http://circ.ahajournals.org/content/109/10/1230.long
*  Enhanced Lymph Vessel Density, Remodeling, and Inflammation Are Reflected by Gene Expression Signatures in Dermal Lymphatic...
Quantitative reverse transcriptase-polymerase chain reaction.. One microgram of amplified total RNA was transcribed into cDNA ... by Superscript II Reverse Transcriptase (Invitrogen no. 18064-014). A complete probe list is summarized in Supplementary Table ... ndLECs were selected, and qPCR reactions were performed in triplicate, with total RNA extracted from dLECs and ndLECs (n = 4 in ... TNF-α-treated LEC monolayers showed twofold-enhanced macrophage adhesion in vitro, which could be completely reversed by anti- ...
http://diabetes.diabetesjournals.org/content/62/7/2509.full
*  Plus it
Reverse Transcriptase-Polymerase Chain Reaction.. Total RNA was extracted from AdhPEPT1-EYFP-transduced cells and frozen ... reverse transcription-polymerase chain reaction. PBS. phosphate-buffered saline. EYFP. enhanced yellow fluorescent protein. ... Total RNA was reverse-transcribed and amplified by polymerase chain reaction (PCR) by using specific primers for hPEPT1-EYFP. ... RT (−) represents the control lacking reverse transcriptase enzyme. B, Fluorescence (a) and phase-contrast (b) micrographs of ...
http://jpet.aspetjournals.org/content/305/1/40
*  Human Genetic Relevance and Potent Antitumor Activity of Heat Shock Protein 90 Inhibition in Canine Lung Adenocarcinoma Cell...
Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to identify the presence of mRNA for NKX2-1, also known as ... RNA Isolation and Reverse-Transcriptase Polymerase Chain Reaction. Total RNA was isolated from both cell lines using the ... B) Reverse transcriptase cDNA transcripts of HSP70 and three HSP90 isoforms in BACA and CLAC cell lines. NDUFA1 serves as a ... A) Reverse transcriptase cDNA transcripts of NKX2-1 (lung cancer cell marker) and HSP90 client proteins in BACA and CLAC cell ...
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0142007
*  Tranilast Suppresses Vascular Chymase Expression and Neointima Formation in Balloon-Injured Dog Carotid Artery | Circulation
Quantitative Reverse-Transcriptase Polymerase Chain Reaction Analysis. Chymase and ACE mRNA levels of carotid arteries were ... determined with quantitative reverse-transcriptase polymerase chain reaction (PCR) analysis described previously with ... polymerase chain reaction. Percutaneous transluminal coronary angioplasty (PTCA) is an established therapy for obstructive ... acetonitrile and 10 μL of acetic acid and then fractionated by reverse-phase high-performance liquid chromatography. Common ...
http://circ.ahajournals.org/content/99/8/1084
*  Frontiers | STIM Proteins and Orai Ca2+ Channels Are Involved in the Intracellular Pathways Activated by TLQP-21 in RAW264.7...
Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Total RNA was extracted from RAW264.7 cells using EuroGOLD Trifast ... Total RNA was transcribed to cDNA using M-MLV Reverse Transcriptase (Invitrogen, Carlsbad, CA, United States). cDNA was ... C3aR reverse: 5′-AAATACGGGCACACACATCA-3′; GAPDH forward: 5′-GCCATCAACGACCCCTTCATTG-3′; GAPDH reverse: 5′- ... were synthesized using conventional solid phase synthesis and then purified on a C18 reversed phase column. Each peptide was ...
https://www.frontiersin.org/articles/10.3389/fphar.2018.01386/full