*  "Isolation of infectious xenotropic mouse type c virus by transfection " by E M. Scolnick and S J. Bumgarner
Scolnick, E M. and Bumgarner, S J., "Isolation of infectious xenotropic mouse type c virus by transfection of a heterologous cell with dna from a transformed mouse cell." (1975). Subject Strain Bibliography 1975. 2094 ...
*  The murine stem cell virus (MSCV) promoter exhibits activity in mouse - Polo-Like Kinases and Aurora Kinases in Cancer Therapy
The murine stem cell virus (MSCV) promoter exhibits activity in mouse hematopoietic cells and embryonic stem cells. respectively. The strength of the GFP fluorescence in the body was comparable to the proportion of GFP-positive leukocytes. Moreover, the rate of recurrence of the GFP-expressing leukocytes was significantly correlated with the frequency of GFP-expressing Purkinje cells. These results suggest that the MSCV promoter is useful for preferentially expressing a transgene in Purkinje cells. In addition, the proportion of transduced leukocytes in the peripheral circulation reflects the expression level of the transgene in Purkinje Rabbit Polyclonal to PPP2R3B cells, which can be used as a way to monitor transgene expression properties in the cerebellum without invasive techniques. Introduction The Moloney murine leukemia virus (MoMLV)-based retrovirus vector has been widely used to transfer genes into dividing eukaryotic cells [1]. MoMLV and MoMLV-derived retroviral vectors are not active ...
*  pASSEMBLE™ 10A1 Retroviral Packaging System | Canvax Biotech
pASSEMBLE™ 10A1 Retroviral Packaging System includes a unique packaging vector with gag, pol and env from different viruses confering a tropism in the cell to be infected.
*  "Molecular cloning of a highly leukemogenic, ecotropic retrovirus from " by J Lenz, R Crowther et al.
Lenz, J; Crowther, R; Klimenko, S; and Haseltine, W, "Molecular cloning of a highly leukemogenic, ecotropic retrovirus from an akr mouse." (1982). Subject Strain Bibliography 1982. 4247 ...
*  "C-type particles in primary and transplanted lung tumors induced in ba" by E Bucciarelli and R Ribacchi
Bucciarelli, E and Ribacchi, R, "C-type particles in primary and transplanted lung tumors induced in balb/c mice by hydrazine sulfate, electron microscopic and immuno- diffusion studies." (1972). Subject Strain Bibliography 1972. 2601 ...
*  Investigation of the role of target cell factors in retrovirus transduction - pdf descargar
Investigation of the role of target cell factors in retrovirus transduction. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
*  Expression of the nlsLacz gene in dendritic cells derived from retrovirally transduced peripheral blood CD34+ cells |...
BACKGROUND AND OBJECTIVE: Gene transfer and expression of exogenous genetic information coding for an immunogenic protein in antigen presenting cells (APCs) can promote an immune response. This was investigated by retroviral transfer of a marker gene into CD34+ derived APCs. DESIGN AND METHODS: To achieve long term expression of a specific transgene in APCs, G-CSF mobilized peripheral blood CD34+ cell populations were retrovirally transduced with the bacterial nlsLacZ, a marker gene used here as a model, in the presence of IL-3, IL-6, GM-CSF and SCF prior to being induced to differentiate into dendritic and macrophage cells by GM-CSF and TNF-a. RESULTS: Addition of IL-4 was found to induce dendritic differentiation preferentially by inhibiting proliferation and differentiation of the macrophage lineage. As assessed by X-Gal staining, LacZ gene expression was observed in cells from both the dendritic lineage (CD1a+/CD14-) which still exhibits the highest immunostimulatory activity in mixed ...
*  Retroviral delivery system
Connect with Featured Speakers and Experts from USA, Europe, Middle East and Asia Pacific at Retroviruses and Novel drug Conferences, Emerging Retroviruses and Novel drug Conferences, Retroviruses and Novel drug Meetings scheduled from July 27-28, 2017 Vancoure,Canada
*  Plus it
Cell Lines, Plasmids, Antibodies, and Compounds. The β1-deficient GE11 cells were described previously (Gimond et al., 1999). Cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum and antibiotics. LZRS bicistronic retroviral expression plasmids encoding SrcYF, RasGV, and human integrins β1 and β3 were described previously (Danen et al., 2002; Huveneers et al., 2007b). To ensure identical expression of SrcYF (c-Src[Y530F]) or RasGV (H-Ras[G12V]) in cell lines, we first generated GESrcYF and GERasGV single cell clones and subsequently expressed β1 or β3 integrin subunits using retroviral transduction and bulk sorting by FACS.. The following antibodies were used: mouse monoclonal anti-p53 (Pab240; Santa Cruz Biotechnology) and anti-α-tubulin (DM1A, Sigma); rabbit polyclonal anti-p21 (C-19, Santa Cruz Biotechnology, Santa Cruz, CA), anti-phospho-p53 (Ser15; Cell Signaling Technology Inc., Danvers, MA), anti-caspase-3 (Cell Signaling Technology Inc.), ...
*  retrovirus | This Week in Virology
Ted and Welkin inform the TWiV team how the evolution of ancient retroviruses can be inferred by their sequences in the genomes of modern mammals, and join in a discussion of virus dispersal during different methods for drying hands.. ...
*  MMTV and Breast Cancer - erv
Sorry, Hitch. Religion doesnt poison *everything*.. Thanks to the bumbling efforts of 'respected' scientists like Judy Mikoivts, we now know that its not religion, but mouse DNA, that poisons everything.. Relatively new readers of ERV are well aware of the fact that all efforts to connect XMRV to any human pathogen have ended up being the result of contamination. Though patient samples from the WPI were contaminated with plasmid in a rather convenient (strategic?) manner, others attempting to find the virus have universally determined that their 'positive' results are actually the result of mouse DNA contaminating their samples, their reagents, even their Qiagen columns. 'XMRV' only momentarily appeared to be a human pathogen because mouse DNA poisons everything.. But only looooooong-time readers of ERV know that this is not new at all. Though XMRV True Believers like to pretend that the inability to connect a mouse retrovirus to their pet disease is a conspiracy, the fact of the matter is, ...