Isolation, sequencing, and the genomic organization of the reduced folate carrier gene in the murine system
... will be initiated to determine the tissue specific expression of the RFC gene in relation to the folate binding proteins. The ... The reduced folate carrier (RFC) delivers 5-methyltetrahydrofolate from the folate receptors to the cytoplasm of the cell. As a ... Isolation, sequencing, and the genomic organization of the reduced folate carrier gene in the murine system. ... Greer, Kimberly Ann (1996). Isolation, sequencing, and the genomic organization of the reduced folate carrier gene in the ...http://oaktrust.library.tamu.edu/handle/1969.1/ETD-TAMU-1996-THESIS-G74
The spectrum of mutations in the PCFT gene, coding for an intestinal folate transporter, that are the basis for hereditary...
Also, since reduced folate carrier (RFC) is expressed along the entire small intestine, with sufficient folate intake, some ... This same gene was previously reported to be a heme carrier protein (HCP1) that mediates heme-iron absorption,7 but its major ... Role of the amino acid 45 residue in reduced folate carrier function and ion-dependent transport as characterized by site- ... Structure and regulation of the murine reduced folate carrier gene: identification of four noncoding exons and promoters and ...http://www.bloodjournal.org/content/110/4/1147.long?sso-checked=true
Folic Acid and Tetrahydrofolates in Cell Culture | China-Mainland | Sigma-Aldrich
... protein-free and animal protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal ... Cells use several transport systems to take up folates. One route involves a reduced folate carrier system (RFC). Other folate ... Sigma's Cell Culture Media Expert provides in depth discussion of this and other serum-free and protein-free media supplements ... One-carbon groups associated with active folates are bound to atoms N-5 and/or N-10 of the molecule. Folic acid is reduced in ...https://www.sigmaaldrich.com/china-mainland/zh/life-science/cell-culture/learning-center/media-expert/folic-acid.html
SLC19A2 gene - Genetics Home Reference - NIH
SLC19A2 purified MaxPab mouse polyclonal antibody (B01P) - (H00010560-B01P) - Products - Abnova
SLC19A2 (NP_008927.1, 1 a.a. ~ 497 a.a) full-length human protein. (H00010560-B01P) - Products - Abnova ... Mouse polyclonal antibody raised against a full-length human SLC19A2 protein. ... OTTHUMP00000033497,high affinity thiamine transporter,reduced folate carrier protein (RFC) like,solute carrier family 19, ... This gene encodes the thiamin transporter protein. Mutations in this gene cause thiamin-responsive megaloblastic anemia ...http://www.abnova.com/products/products_detail.asp?catalog_id=H00010560-B01P
SLC19A1 - Wikipedia
Liu XY, Matherly LH (August 2002). "Analysis of membrane topology of the human reduced folate carrier protein by hemagglutinin ... "The reduced folate carrier (SLC19A1) c.80G>A polymorphism is associated with red cell folate concentrations among women". ... Solute carrier family 19 (folate transporter), member 1, also known as SLC19A1 or RFC1, is a protein which in humans is encoded ... Dixon KH, Lanpher BC, Chiu J, Kelley K, Cowan KH (January 1994). "A novel cDNA restores reduced folate carrier activity and ...https://en.wikipedia.org/wiki/SLC19A1
Pralatrexate - DrugBank
... cancer cells is based upon the observation that cancer cells generally have an overexpression of reduced folate carrier protein ... Reduced folate carrier activity. Specific Function. Transporter for the intake of folate. Uptake of folate in human placental ... This carrier protein allows the entrance of pralatrexate into the cell. Upon entering the cell, folypolyglutamate synthase FPGS ... Protein binding. 67 - 86% bound to plasma protein, albumin is the major binder. Does not significantly displace substrates from ...https://www.drugbank.ca/drugs/DB06813
Functional Characterization of Human Proton-Coupled Folate Transporter/Heme Carrier Protein 1 Heterologously Expressed in...
... reduced folate carrier; PCFT, proton-coupled folate transporter; HCP, heme carrier protein; hPCFT, human proton-coupled folate ... Reduced folate carrier (RFC) 1 is the first cloned transporter that was found to transport folates (Williams et al., 1994; ... Proton-coupled folate transporter (PCFT)/heme carrier protein (HCP) 1 is another folate transporter identified quite recently. ... Thus, hPCFT/HCP1 was suggested to have a characteristic of a folate transporter that prefers folate to reduced folates (THF and ...http://jpet.aspetjournals.org/content/322/2/469
Spina Bifida Research Bibliography: 08/01/2007 - 09/01/2007
For this reason, a common polymorphism in the RFC-1 gene encoding the reduced folate carrier protein (A80 G) could also be an ... Transport of folates in mammalian cells occurs by a carrier-mediated mechanism. The human folate carrier (RFC-1) gene has been ... De Marco P, Calevo MG, Moroni A, Merello E, Raso A, Finnell RH, Zhu H, Andreussi L, Cama A, Capra V. Reduced folate carrier ... Evaluation of genetic variants in the reduced folate carrier and in glutamate carboxypeptidase II for spina bifida risk ...http://spina-bifida-bibliography.blogspot.com/2007/08/
Pralatrexate プララトレキサート « New Drug Approvals
... researchers believe that pralatrexate selectively enters cells expressing reduced folate carrier type 1 (RFC-1), a protein that ... cancer cells is based upon the observation that cancer cells generally have an overexpression of reduced folate carrier protein ... It is selective for the reduced folate carrier type 1. Its IC50 is , 300 nM in some cell lines. ... "reduced folate carrier type 1" or "RFC-1"). Further research showed that when normal cells evolve into cancerous cells they ...https://newdrugapprovals.org/2018/03/16/pralatrexate-%E3%83%97%E3%83%A9%E3%83%A9%E3%83%88%E3%83%AC%E3%82%AD%E3%82%B5%E3%83%BC%E3%83%88/comment-page-1/
THE HEDGEHOG PATHWAY IN MALIGNANT MESOTHELIOMA - PDF
NADPH oxidase Phosphate buffered saline Phosphoinositide 3-kinase Protein kinase A Patched RFC1 Reduced folate carrier protein ... Protein Trafficking/Targeting (8.1) Lecture 8 Protein Trafficking/Targeting Protein targeting is necessary for proteins that ... Lecture 8. Protein Trafficking/Targeting. Protein targeting is necessary for proteins that are destined to work outside the ... 1. What is a ligand? a. An integral membrane protein associated with G proteins b. A DNA-binding protein that alters gene ...http://docplayer.net/997469-The-hedgehog-pathway-in-malignant-mesothelioma.html
Table of Contents - September 01, 2005, 68 (3) | Molecular Pharmacology
The Reduced Folate Carrier Gene Is a Novel Selectable Marker for Recombinant Protein Overexpression Lilah Rothem, Bluma Berman ... Enhanced CCAAT/Enhancer-Binding Protein β-Liver-Enriched Inhibitory Protein Production by Oltipraz, Which Accompanies CUG ... Poly(C) Binding Protein Family Is a Transcription Factor in μ-Opioid Receptor Gene Expression Sung-Su Kim, Krishan K. Pandey, ... Guanine Nucleotide Exchange-Independent Activation of Gs Protein by β2-Adrenoceptor Özlem Uğur, Şükrü Sadik Öner, Paola ...http://molpharm.aspetjournals.org/content/68/3
The roles of Sp1, Sp3, USF1/USF2 and NRF-1 in the regulation and three-dimensional structure of the Fragile X mental...
We also show that, like Sp1 and E-box-binding proteins such as USF1 and USF2, NRF-1 causes DNA bending, in this case producing ... We can reconcile these observations with the positive effect of Sp1 and Sp3 if protein-induced bending acts, at least in part, ... The combined effect of the four protein-induced bends on promoter geometry is the formation of a highly compact arch-like ... In the present study, we show that Sp1 (specificity protein 1) and Sp3 are also strong positive regulators of FMR1 promoter ...https://www.semanticscholar.org/paper/The-roles-of-Sp1%2C-Sp3%2C-USF1%2FUSF2-and-NRF-1-in-the-X-Kumari-Gabrielian/2f356b64008c7157c2f8684b25b9e822a946ea1a
... reduced folate carrier; MRP, multidrug resistance-associated protein; BCRP, breast cancer resistance protein; PAH, p- ... presumably reduced folate carrier (RFC)-1 (Slc19a1), a transporter of reduced folate and its derivatives (Nozaki et al., 2004 ... In addition, recently, proton-coupled folate transporter/heme carrier protein 1 (PCFT/HCP1) was also identified as a novel MTX ... drugs in the renal uptake process based on the contribution of organic anion transporters and reduced folate carrier. J ...http://jpet.aspetjournals.org/content/322/3/1162
In breast cancer cell lines, the protein expression level of reduced folate carrier 1 (RFC1) was directly correlated with ... Absolute protein expression levels of 90 membrane proteins were examined by quantitative targeted absolute proteomics using ... In stomach cancer cell lines, the protein expression level of multidrug resistance-associated protein 1 (MRP1) was inversely ... Membrane transporter proteins may influence the sensitivity of cancer cells to anticancer drugs that can be recognized as ...http://molpharm.aspetjournals.org/content/83/2/490
Pralatrexate - Wikipedia
... researchers believe that pralatrexate selectively enters cells expressing reduced folate carrier type 1 (RFC-1), a protein that ... "reduced folate carrier type 1" or "RFC-1"). Further research showed that when normal cells evolve into cancerous cells they ... researchers at Memorial Sloan Kettering Cancer Center discovered that cancerous cells take in natural folate through a protein ... Pralatrexate is an antifolate (a folate analogue metabolic inhibitor) designed to accumulate preferentially in cancer cells. ...https://en.wikipedia.org/wiki/Pralatrexate
Alimta (pemetrexed disodium) dose, indications, adverse effects, interactions... from PDR.net
Pemetrexed is taken into cells by membrane carriers (e.g., reduced folate carrier and membrane folate binding protein transport ... Impaired cellular transport of physiologic reduced folates via reduced folate carriers leads to smaller intracellular folate ... Interestingly, cells resistant to methotrexate due to impaired cellular transport via reduced folate carriers may retain ... Glucarpidase: (Moderate) Potential exogenous substrates of glucarpidase include reduced folates and folate antimetabolites. ...https://www.pdr.net/drug-summary/Alimta-pemetrexed-disodium-2260.1332
Use of novel genetic mouse models to investigate the health benefits of folate in colon cancer | Cancer Research
... we have generated genetically modified mouse models by targeting the reduced folate carrier (RFC1) and folate binding protein ( ... Consumption of a low folate diet significantly reduced (3-4 fold) plasma and tissue folate levels in all animal models, ... These data demonstrate that the ablation of two receptor/carrier-mediated pathways for folate transport increases the risk for ... Compound heterozygous mice (Folbp1+/-;RFC1+/-) mice fed an adequate folate diet exhibited a reduction in plasma folate ...http://cancerres.aacrjournals.org/content/67/9_Supplement/LB-78
Clinical significance of polyglutamylation in primary central nervous system lymphoma | Acta Neuropathologica Communications |...
... whereas enhancement of FPGS expression by NaBu treatment significantly reduced this relief effect. These findings suggested ... Polyglutamylation is a reversible protein modification with a high occurrence rate in tumor cells. MTX incorporated into cells ... Decreased expression of the reduced folate carrier, a transport protein, was associated with impaired MTX transport and was ... of acquired methotrexate resistance in acute lymphocytic leukemia and is associated with decreased reduced folate carrier ...https://actaneurocomms.biomedcentral.com/articles/10.1186/s40478-018-0522-4
Structural and functional analysis of the human TAF1/DYT3 multiple transcript system, Mammalian Genome | 10.1007/s00335-007...
Roles of USF, Ikaros and Sp proteins in the transcriptional regulation of the human reduced folate carrier B promoter ... Thirty-eight exons code for TATA box binding protein associated factor I (TAF1). Five downstream exons (d1-d5) of yet unknown ... Reduced neuron-specific expression of the TAF1 gene is associated with X-linked dystonia-parkinsonism ... Zinc finger-mediated protein interactions modulate Ikaros activity, a molecular control of lymphocyte development ...https://www.deepdyve.com/lp/springer_journal/structural-and-functional-analysis-of-the-human-taf1-dyt3-multiple-Ou5L5ZNSx0
Clinical manifestations of hematologic and oncologic disorders in patients with Down syndrome.
The reduced folate carrier gene, localized on chromosome 21, encodes the transmembrane protein which transports intracellularly ... Reduced folate carrier expression in acute lymphoblastic leukemia: A mechanism for ploidy but not lineage differences in ... The high expression of the reduced folate carrier gene may also account for increased methotrexate-associated toxicity of DSALL ... have an increased expression of the reduced folate carrier gene and intracellular transport of methotrexate, therefore, ...https://www.docme.ru/doc/1916805/clinical-manifestations-of-hematologic-and-oncologic-diso..
Protocols and Video Articles Authored by Ian Parker
... of the water-soluble vitamin folic acid in mammalian cells is via a plasma membrane protein known as the reduced folate carrier ... Intracellular Trafficking and Membrane Targeting Mechanisms of the Human Reduced Folate Carrier in Mammalian Epithelial Cells ... Therefore, we designed a series of fusion proteins of the human RFC (hRFC) with green fluorescent protein to image the ... protein inhibitor pertussis toxin, and pretreatment with lithium to deplete membrane inositol lipids. We show that G-protein- ...https://www.jove.com/author/Ian_Parker
Pemetrexed Coupon 2018 & 80% off Alimta Discount Card · RX24 Drugs
Pemetrexed is transported into cells by both the reduced folate carrier and membrane folate binding protein transport systems. ... all folate-dependent enzymes involved in the de novo biosynthesis of thymidine and purine nucleotides. ... is an antifolate containing the pyrrolopyrimidine-based nucleus that exerts its antineoplastic activity by disrupting folate- ...https://rx24drugs.com/coupon/pemetrexed-prescription/
SHMT1 Gene - GeneCards | GLYC Protein | GLYC Antibody
Protein Coding), Serine Hydroxymethyltransferase 1, including: function, proteins, disorders, pathways, orthologs, and ... response and toxicity in relation to genetic polymorphisms and dihydrofolate reductase and reduced folate carrier 1 expression. ... Protein details for SHMT1 Gene (UniProtKB/Swiss-Prot). Protein Symbol:. P34896-GLYC_HUMAN. Recommended name:. Serine ... Protein Expression for SHMT1 Gene. See protein expression from ProteomicsDB, MOPED, PaxDb, and MaxQB ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=SHMT1
Folate-binding protein - Wikipedia
Folate-binding protein (FBP) are proteins that bind folate, typically the folate receptors and reduced folate carrier. It can ... FBP can refer to protein(s) (e.g. extracted from cow's milk) used to do folic acid assays. "The role of folate receptor a in ... www.scrippslabs.com/enzymes-biochemicals-folate-binding-protein/. ... "Folate-binding Protein Is a Marker for Ovarian Cancer". 1991. http://www.marketwatch.com/story/galena-biopharma-to-present-at- ...https://en.wikipedia.org/wiki/Folate-binding_protein