Expression of cre recombinase in pigment cells - InfoscienceExpression of cre recombinase in pigment cells - Infoscience

A construct was generated where Cre recombinase is controlled by the promoter of the mouse dopachrome tautomerase (Dct) gene. ... Following breeding to two reporter mouse strains, we detected Cre recombinase activity in telencephalon, melanoblasts, and ... cell-specific Cre transgenic line has been reported and we present and discuss our first results on use of Cre recombinase in ... Expression of cre recombinase in pigment cells Guyonneau, L. ; Rossier, A. ; Richard, C. ; Hummler, E.[...] ...
more infohttps://infoscience.epfl.ch/record/115149

IJERPH  | Free Full-Text | Development of a Recombinase Polymerase Amplification Assay for the Detection of Pathogenic...IJERPH | Free Full-Text | Development of a Recombinase Polymerase Amplification Assay for the Detection of Pathogenic...

In this study we report the development and evaluation of a novel isothermal recombinase polymerase amplification assay (RPA) ... Recombinase Polymerase Amplification: Reagents and Conditions. To perform the RPA, the TwistAmp exo kit (TwistDX, Cambridge, UK ... In addition to a regular DNA polymerase, this method employs a recombinase enzyme, single-stranded DNA binding proteins and ... Development of a Recombinase Polymerase Amplification Assay for the Detection of Pathogenic Leptospira ...
more infohttp://www.mdpi.com/1660-4601/11/5/4953/htm

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive ControlDevelopment of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

genetics; recombinase polymerase amplification; isothermal amplification; quantitative; diagnostic; More... HIV-1; viral load ... Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control. ... "Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control." Journal of ... a detailed protocol for developing and implementing a real-time quantitative recombinase polymerase amplification assay (qRPA ...
more infohttps://scholarship.rice.edu/handle/1911/94335

Development of the recombinase-based in vivo expression technology in Streptococcus thermophilus and validation using the...Development of the recombinase-based in vivo expression technology in Streptococcus thermophilus and validation using the...

Rao, S.P., Camacho, L., Huat Tan, B., Boon, C., Russel, D.G., Dick, T. and Pethe, K. (2008) Recombinase-based reporter system ... Development of the recombinase-based in vivo expression technology in Streptococcus thermophilus and validation using the ... Development of the recombinase-based in vivo expression technology in Streptococcus thermophilus and validation using the ...
more infohttp://onlinelibrary.wiley.com/doi/10.1111/jam.12376/references

STORRE: Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene...STORRE: Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene...

Yehia N, Arafa A, El Wahed AA, El-Sanousi AA, Weidmann M & Shalaby MA (2015) Development of reverse transcription recombinase ... In this study, a qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of ... Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection. ...
more infohttp://dspace.stir.ac.uk/handle/1893/22809

2016 Annual Meeting | Evaluation of Recombinase Polymerase Amplification (RPA) Isothermal Amplification Diagnostic Assay for <i...2016 Annual Meeting | Evaluation of Recombinase Polymerase Amplification (RPA) Isothermal Amplification Diagnostic Assay for <i...

Evaluation of Recombinase Polymerase Amplification (RPA) Isothermal Amplification Diagnostic Assay for Phytophthora ramorum ... Evaluation of Recombinase Polymerase Amplification (RPA) Isothermal Amplification Diagnostic Assay for Phytophthora ramorum.. J ... A previously published Recombinase Polymerase Amplification (RPA) Isothermal Amplification Assay for detection of P. ramorum ...
more infohttp://www.apsnet.org/meetings/Documents/2016_meeting_abstracts/aps2016_676.htm

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control | Protocol ...Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control | Protocol ...

Molecular Evolution of the Tre Recombinase. Published 5/29/2008. Principles of Site-Specific Recombinase (SSR) Technology…. ... Loo, J. F. C., Lau, P. M., Ho, H. P., Kong, S. K. An aptamer-based bio-barcode assay with isothermal recombinase polymerase ... Euler, M., et al. Development of a panel of recombinase polymerase amplification assays for detection of biothreat agents. ... Abd El Wahed, A., et al. A portable reverse transcription recombinase polymerase amplification assay for rapid detection of ...
more infohttps://www.jove.com/video/52620/udvikling-af-en-kvantitativ-rekombinase-polymerase?language=Danish

Recombinase Polymerase Amplification: An Isothermal Technique Suitable for a Range of Applications - Evaluation of RPA Volume...Recombinase Polymerase Amplification: An Isothermal Technique Suitable for a Range of Applications - Evaluation of RPA Volume...

TwistDx s proprietary Recombinase Polymerase Amplification (RPA) method addresses many of the challenges faced by PCR methods ... TwistDxs proprietary Recombinase Polymerase Amplification (RPA) method addresses many of the challenges faced by PCR methods ... Application Note: Recombinase Polymerase Amplification: An Isothermal Technique Suitable for a Range of Applications - ...
more infohttp://www.selectscience.net/application-articles/recombinase-polymerase-amplification--an-isothermal-technique-suitable-for-a-range-of-applications---evaluation-of-rpa-volume-tolerance/?artID=43468

Get PDF - Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of...Get PDF - Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of...

Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides ... Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the ... Recombinase polymerase amplification combined with a lateral flow dipstick for rapid and visual detection of Schistosoma ... Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ...
more infohttps://eurekamag.com/research/065/464/065464095.php

A recombinase polymerase amplification assay for detection of the heterogeneous sequences encompassing Japanese Encephalitis...A recombinase polymerase amplification assay for detection of the heterogeneous sequences encompassing Japanese Encephalitis...

Recombinase polymerase amplification (RPA) is a relatively new isothermal amplification technique amenable for use in low- ... Recombinase polymerase amplification (RPA) is a relatively new isothermal amplification technique amenable for use in low- ... A recombinase polymerase amplification assay for detection of the heterogeneous sequences encompassing Japanese Encephalitis ... A recombinase polymerase amplification assay for detection of the heterogeneous sequences encompassing Japanese Encephalitis ...
more infohttp://research.usc.edu.au/vital/access/manager/Repository/usc:14314

Separation of Recombination and SOS Response in  RecA Suggests LexA Interaction Sites | proLékaře.czSeparation of Recombination and SOS Response in RecA Suggests LexA Interaction Sites | proLékaře.cz

The bacterial recombinase protein RecA is pivotal to DNA repair [1]-[4] and to orchestrate the bacterial DNA damage response ( ... Such marked defect in recombinase function was previously reported by a point mutation involving Gly301 to Asp in the CTD [95] ... Under recombinase activity, recombination frequencies equivalent to 100% are indicated by 6+. Under LexA cleavage, if the ... Thus, overall, all these ET site-2 mutations have more severe impact on the recombinase activity than on the SOS response. The ...
more infohttps://www.prolekare.cz/casopisy/plos-genetics/2011-9/separation-of-recombination-and-sos-response-in-reca-suggests-lexa-interaction-sites-45400

Cre-Lox RecombinationCre-Lox Recombination

The Cre protein is a site-specific DNA recombinase. It can catalyze the recombination of DNA between specific sites in a DNA ... The Cre protein is a site-specific DNA recombinase. It can catalyze the recombination of DNA between specific sites in a DNA ...
more infohttp://www.abnova.com/abvideo/Cre-Lox-Recombination.html

Negative co-dominant inhibition of recA protein function: Biochemical properties of the recA1, recA13 and recA56 proteins and...Negative co-dominant inhibition of recA protein function: Biochemical properties of the recA1, recA13 and recA56 proteins and...

TY - JOUR. T1 - Negative co-dominant inhibition of recA protein function. T2 - Biochemical properties of the recA1, recA13 and recA56 proteins and the effect of recA56 protein on the activities of the wild-type recA protein function in vitro. AU - Lauder, Scott D.. AU - Kowalczykowski, Stephen C.. PY - 1993. Y1 - 1993. N2 - We have investigated the biochemical properties of several Escherichia coli mutant recA proteins that display a null phenotype. These are the recA1, recA13 and recA56 proteins, each of which carries a single missense mutation. These proteins all share a common defect which is the inability to adopt the high affinity DNA binding state normally elicited by the nucleotide cofactor ATP. Consequently, other than the ability to bind ssDNA, they possess none of the in vitro enzymatic activities of recA protein. However, each protein has characteristics that are unique, leading to the conclusion that the observed mutant phenotypes arise through fundamentally different mechanisms. ...
more infohttps://ucdavis.pure.elsevier.com/en/publications/negative-co-dominant-inhibition-of-reca-protein-function-biochemi

Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site...Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site...

This protein has substantial sequence similarity to the lambda integrase family of site-specific recombinases and is probably ... a member of the lambda integrase family of site-specific recombinases. Journal of Bacteriology, 172(12), pp. 6973-6980. (doi: ... a member of the lambda integrase family of site-specific recombinases ... the cer recombinase. The xerC gene is expressed as part of a multicistronic unit that includes the dapF gene and two other open ...
more infohttp://eprints.gla.ac.uk/133520/

RECOMBINATIONAL CLONING USING NUCLEIC ACIDS HAVING RECOMBINATION SITES - Patent applicationRECOMBINATIONAL CLONING USING NUCLEIC ACIDS HAVING RECOMBINATION SITES - Patent application

Such recombinases are known. In many cases the purification of such other recombinases has been described in the art. In cases ... 0005]Site-specific recombinases. Site-specific recombinases are proteins that are present in many organisms (e.g. viruses and ... 0175]Site-specific recombinase: is a type of recombinase which typically has at least the following four activities (or ... 0224]The above recombinases and corresponding recombinase sites are suitable for use in recombination cloning according to the ...
more infohttp://www.patentsencyclopedia.com/app/20090186386

Cre recombinase geneCre recombinase gene

Valeri Krougliak ,krougv01 at doc.mssm.edu, wrote: , , I am looking for a commercial source of the Cre recombinase gene. It , ... Cre recombinase gene. Keith Leppard Keith.Leppard at warwick.ac.uk Wed Oct 30 08:04:23 EST 1996 *Previous message: Cre ...
more infohttp://www.bio.net/bionet/mm/methods/1996-October/050914.html

recombinase genes and viral transferrecombinase genes and viral transfer

... Ralph M Bernstein ralph at ccit.arizona.edu Wed Mar 1 10:27:52 EST 1995 *Previous message ... these are the recombinase activating genes. they are essential for immune system function-t and b cell rearrangement. a ...
more infohttp://www.bio.net/bionet/mm/mol-evol/1995-March/002482.html

recA recombinase A [Desulfovibrio vulgaris str. Hildenborough] - Gene - NCBIrecA recombinase A [Desulfovibrio vulgaris str. Hildenborough] - Gene - NCBI

recA; recombinase A; Provisional. cd00983. Location:13 → 337. recA; RecA is a bacterial enzyme which has roles in homologous ... recombinase A. YP_010311.1. *catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake ... recombinase A. Locus tag. DVU1090. Gene type. protein coding. RefSeq status. REVIEWED. Organism. Desulfovibrio vulgaris str. ... YP_010311.1 recombinase A [Desulfovibrio vulgaris str. Hildenborough]. See identical proteins and their annotated locations for ...
more infohttps://www.ncbi.nlm.nih.gov/gene/2794069

Chromosomal Manipulation by Site-Specific Recombinases and Fluorescent Protein-Based VectorsChromosomal Manipulation by Site-Specific Recombinases and Fluorescent Protein-Based Vectors

... we developed a Cre-mediated chromosomal recombination system using fluorescent proteins and various site-specific recombinases ...
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0009846

Targeted retrieval of recombinase activity by trans-splicingTargeted retrieval of recombinase activity by trans-splicing

In this process the activity of the Cre-recombinase is dependent on the control of a cell-specific promotor. This method ... in which the enzyme Cre-recombinase achieves recombination of DNA at specific target sequences, the so-called loxP-sites. ...
more infohttp://www.innovations-report.com/html/reports/technology-offerings/targeted-retrieval-recombinase-activity-trans-146830.html

Bacteriophage P1 Cre recombinase peptide (ab41101) | AbcamBacteriophage P1 Cre recombinase peptide (ab41101) | Abcam

Buy our Bacteriophage P1 Cre recombinase peptide. Ab41101 is a Synthetic peptide for ab40011. Abcam provides free protocols, ... Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific for a 34 bp DNA sequence ( ... Bacteriophage P1 Cre recombinase peptide. See all Cre recombinase proteins and peptides. ...
more infohttps://www.abcam.com/bacteriophage-p1-cre-recombinase-peptide-ab41101.html

Recombinase Knock-in or Transgenic Allele Activity/Specificity Data SubmissionRecombinase Knock-in or Transgenic Allele Activity/Specificity Data Submission

Recombinase activity data Data on recombinase (cre) specificity/activity.. Recombinase Activity Data:. Please download Excel ... Enter recombinase knock-in or transgene data: * = required field. Suggest symbol and/or name for the recombinase knock-in or ... The Recombinase (cre) Portal accepts data about cre-bearing transgenes and knock-ins as well as non-cre recombinase (Flg, Dre, ... For an inducible recombinase, identify the inducing agent: Strain background in which the recombinase allele/transgene was ...
more infohttp://www.informatics.jax.org/mgihome/submissions/recombinase_submission.cgi

Principles of Site-Specific Recombinase (SSR) Technology | ProtocolPrinciples of Site-Specific Recombinase (SSR) Technology | Protocol

The advent of site-specific recombinase (SSR) technology and the Cre/lox system has led to numerous advances in molecular ... Molecular Evolution of the Tre Recombinase. Published 5/29/2008. Interview: HIV-1 Proviral DNA Excision Using an Evolved ... Cre recombinase is a Type I topoisomerase that catalyzes site-specific recombination of DNA between two loxP (locus of X-over ... Site-specific recombinases are proteins that bind to distinct DNA target sequences. The Cre/lox system was first described in ...
more infohttps://www.jove.com/video/718/principles-of-site-specific-recombinase-ssr-technology

ASMscience | Site-specific DNA Inversion by Serine RecombinasesASMscience | Site-specific DNA Inversion by Serine Recombinases

A dedicated recombinase, sometimes with the aid of additional regulatory or DNA architectural proteins, catalyzes the inversion ... This chapter discusses site-specific DNA inversion reactions mediated by the serine recombinase family of enzymes and focuses ... These include the steps leading to the formation of the active recombination complex (invertasome) containing the recombinase ... Serine recombinase subfamilies. The domain architectures of serine recombinase subfamily members denoting the ∼100 amino acid ...
more infohttp://www.asmscience.org/content/journal/microbiolspec/10.1128/microbiolspec.MDNA3-0047-2014

xerD - Tyrosine recombinase XerD - Escherichia coli (strain K12) - xerD gene & proteinxerD - Tyrosine recombinase XerD - Escherichia coli (strain K12) - xerD gene & protein

Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. Binds ... IPR010998 Integrase_recombinase_N. IPR004107 Integrase_SAM-like_N. IPR011932 Recomb_XerD. IPR023009 Tyrosine_recombinase_XerC/ ... IPR010998 Integrase_recombinase_N. IPR004107 Integrase_SAM-like_N. IPR011932 Recomb_XerD. IPR023009 Tyrosine_recombinase_XerC/ ... Tyrosine recombinase XerDAdd BLAST. 298. Proteomic databases. PaxDb, a database of protein abundance averages across all three ...
more infohttps://www.uniprot.org/uniprot/P0A8P8&format=html