DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent | SpringerLink
Theyre ideal for expression of human or other mammalian proteins because these systems generate recombinant proteins with more ... Mammalian transient expression systems enable flexible and rapid production of proteins. ... Theyre ideal for expression of human or other mammalian proteins because these systems generate recombinant proteins with more ... Here we describe transient protein production using QMCF Technology (Icosagen, Estonia) that uses specific suspension-adapted ...
Comparison of transplastomic Chlamydomonas reinhardtii and Nicotiana tabacum expression system for the production of a...
The bulk production of recombinant enzymes by either prokaryotic or eukaryotic organisms might contribute to replace ... Hacker DL, De Jesus M, Wurm FM (2009) 25 years of recombinant proteins from reactor-grown cells - where do we go from here? ... In this context, it is worth noting that the production of recombinant proteins by photosynthetic organisms offer both ... Zedler JAZ, Mullineaux CW, Robinson C (2016) Efficient targeting of recombinant proteins to the thylakoid lumen in ...
Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene | SpringerLink
Neumann H, Peak-Chew SY, Chin JW (2008) Genetically encoding N(epsilon)-acetyllysine in recombinant proteins. Nat Chem Biol 4(4 ... Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene. *Jeffery M. Tharp3 & ... Wu B, Wang Z, Huang Y, Liu WR (2012) Catalyst-free and site-specific one-pot dual-labeling of a protein directed by two ... Here, we describe the application of this approach to produce a protein with two distinct chemical functionalites which can be ...
Effects of calcium and EGTA on thiol homeostasis and defense-related enzymes in Cd-exposed chickpea roots | SpringerLink
cDNA characterization and expression of the recombinant protein in Escherichia coli. J Mol Biol 235:1357-1363 ... A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye ... Job C, Rajjou L, Lovigny Y, Belghazi M, Job D (2005) Patterns of protein oxidation in Arabidopsis seeds and during germination ... Zhang H, Lian C, Shen Z (2009) Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza ...
Quantum dot/glycol chitosan fluorescent nanoconjugates | Discover Nano
Fluorescent nanohybrids: quantum-dots coupled to polymer-recombinant protein conjugates for the recognition of biological ... terminated self-assembled monolayers on gold and silver surfaces determines their ability to resist protein adsorption. J Phys ...
Alpha-synuclein suppresses mitochondrial protease ClpP to trigger mitochondrial oxidative damage and neurotoxicity | Acta...
h ClpP recombinant protein (500 ng) was incubated with αSyn WT or αSyn A53T recombinant protein (500 ng, each) in the presence ... b ClpP (10 µg) recombinant protein was incubated with αSyn WT or A53T mutant or CHCHD3 (50 µg, each) recombinant protein. ... i Left: ClpP recombinant protein (10 µM) was incubated with recombinant αSyn WT protein (10, 20 or 50 µM). The fluorescence ... we incubated αSyn WT or A53T recombinant protein with ClpP recombinant protein in vitro, followed by a separation of soluble ...
Inner Ear Organoids: Recapitulating Inner Ear Development in 3D Culture | SpringerLink
Application of various small molecules and recombinant proteins to mouse embryonic stem cells at specific time points in vitro ... Chi N, Epstein JA (2002) Getting your Pax straight: PAX proteins in development and disease. Trends Genet 18(1):41-47 ...
Membrane Protein Production in Lactococcus lactis for Functional Studies | SpringerLink
... expression and study of membrane proteins in heterologous systems remains difficult. Among the bacterial systems available, the ... an efficient cell factory for recombinant protein production and secretion. J Mol Microbiol Biotechnol 14:48-58 ... recent developments in functional expression of membrane proteins. In: Mus-Veteau I (ed) Membrane proteins production for ... heterologous protein production and DNA delivery systems. Protein Expr Purif 79:165-175 ...
MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells | SpringerLink
... and baculovirus-based protein expression in Spodoptera frugiperda cells is a powerful tool to express multiprotein complexes ... Genetic encoding and labeling of aliphatic azides and alkynes in recombinant proteins via a pyrrolysyl-tRNA Synthetase/tRNA(CUA ... The combination of genetic code expansion (GCE) and baculovirus-based protein expression in Spodoptera frugiperda cells is a ... Nikic I, Kang JH, Girona GE, Aramburu IV, Lemke EA (2015) Labeling proteins on live mammalian cells using click chemistry. Nat ...
Identification of a C2H2-type zinc finger transcription factor (ZAT10) from Arabidopsis as a substrate of MAP kinase |...
Mitogen-activated protein kinases (MAPKs or MPKs) are one of the most important and conserved signaling molecules in plants. ... Using in vitro and in vivo protein-protein interaction analyses, we demonstrated that ZAT10 directly interacted with MPK3 and ... ZAT10 was phosphorylated by recombinant Arabidopsis MPK3 and MPK6 in a kinase assay. Furthermore, ZAT10 was also phosphorylated ... Firefly luciferase complementation imaging assay for protein-protein interactions in plants. Plant Physiol 146:368-376 ...
Differential Inhibition of Nav1.7 and Neuropathic Pain by Hybridoma-Produced and Recombinant Monoclonal Antibodies that Target...
... function and pharmacokinetics of monoclonal antibodies and Fc fusion proteins. Eur J Pharm Biopharm 2016, 100: 94-100. ... Liu D, Tseng M, Epstein LF, Green L, Chan B, Soriano B, et al. Evaluation of recombinant monoclonal antibody SVmab1 binding to ... Bang, S., Yoo, J., Gong, X. et al. Differential Inhibition of Nav1.7 and Neuropathic Pain by Hybridoma-Produced and Recombinant ... Du Y, Walsh A, Ehrick R, Xu W, May K, Liu H. Chromatographic analysis of the acidic and basic species of recombinant monoclonal ...
TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms | Cellular and Molecular Life...
d Normalized LC3B-II protein levels for experiments shown in a before PI treatment. b-d Quantifications of LC3B-I/II protein ... c IFM analysis of primary cilia in Tsc1−/− MEFs serum deprived for 48 h in the presence and in the absence of recombinant WNT5A ... Berbari NF, Lewis JS, Bishop GA et al (2008) Bardet-Biedl syndrome proteins are required for the localization of G protein- ... to promote protein synthesis [3, 4]. The phosphorylated form of 40S ribosome protein S6 is thus a marker for mTORC1 activity. ...
Quality of Original and Biosimilar Epoetin Products | Pharmaceutical Research
Separation took place using 100 V. Proteins were visualized by silver staining. PageRuler prestained protein ladder from ... Toyoda T, Itai T, Arakawa T, Aoki KH, Yamaguchi H. Stabilization of human recombinant erythropoietin through interactions with ... The protein content was determined from the UV280 nm signal, using a molar extinction coefficient of 22,600 M−1 cm−1 (12). One ... Schellekens H. Erythropoietic proteins and antibody-mediated pure red cell aplasia: a potential role for micelles. Nephrol Dial ...
Thiazin red as a neuropathological tool for the rapid diagnosis of Alzheimer's disease in tissue imprints | Acta...
Mena R, Edwards P, Perez-Olvera O, Wischik CM (1995) Monitoring pathological assembly of tau and beta-amyloid proteins in ... recognize conformational epitopes on recombinant tau. J Neurosci Res 48:128-132 ... Garcia-Sierra F, Ghoshal N, Quinn B, Berry RW, Binder LI (2003) Conformational changes and truncation of tau protein during ... Wolozin B, Davie P (1987) Alzheimer-related neuronal protein A68: specificity and distribution. Ann Neurol 22:521-526 ...
Reciprocal Interaction of Cancer Stem Cells of Cholangiocarcinoma with Macrophage | Stem Cell Reviews and Reports
Protein samples from tumors derived SORE6± cells were extracted using the mammalian protein extraction reagent M-Per (Promega, ... human recombinant epidermal growth factor (hrEGF) (Gibco, Carlsbad, CA) (20 ng/ml), and bFGF (Gibco, Carlsbad, CA) (10 ng/ml). ... were used to detect individual protein expression. Western blot imaging system was used for testing individual protein ... Additionally, total protein lysates of tumors derived from SORE6± cells were extracted and processed for GFP positivity via ...
Zinc and its effects on oxidative stress in Alzheimer's disease | SpringerLink
Bush AI, Pettingell WH Jr, de Paradis M, Tanzi RE, Wasco W (1994) The amyloid beta-protein precursor and its mammalian ... Draguhn A, Verdorn TA, Ewert M, Seeburg PH, Sakmann B (1990) Functional and molecular distinction between recombinant rat GABAA ... Behl C, Davis JB, Lesley R, Schubert D (1994) Hydrogen peroxide mediates amyloid beta protein toxicity. Cell 77:817-827 ... Ritchie CW, Bush AI, Mackinnon A, Macfarlane S, Mastwyk M et al (2003) Metal-protein attenuation with iodochlorhydroxyquin ( ...
Molecular basis of hypohidrotic ectodermal dysplasia: an update | Journal of Applied Genetics
... caused by mutations of the genes encoding proteins ... Table 1 Mutation of genes encoding proteins involved in TNFα- ... Gaide O, Schneider P (2003) Permanent correction of an inherited ectodermal dysplasia with recombinant EDA. Nat Med 9:614-618 ... May P, Woldt E, Matz RL, Boucher P (2007) The LDL receptor-related protein (LRP) family: an old family of proteins with new ... EDA-A2 binds to XEDAR, which does not require a protein adaptor to react with TRAF proteins. The remaining components are used ...
Diagnosis and management of hypocalcemia | Endocrine
R.B. Payne, A.J. Little, R.B. Williams, J.R. Milner, Interpretation of serum calcium in patients with abnormal serum proteins. ... In hypoparathyroidism, providing the missing hormone with the use of the recombinant human (rh) PTH(1-84) has been recently ... Y. Ramakrishnan, H.C. Cocks, Impact of recombinant PTH on management of hypoparathyroidism: a systematic review. Eur. Arch. Oto ... The development of long-acting recombinant human PTH will probably modify the management of chronic hypoparathyroidism in the ...
Switching Reference Medicines to Biosimilars: A Systematic Literature Review of Clinical Outcomes | Drugs
Biologics were arbitrarily divided into smaller proteins and larger proteins for the sake of simplicity. This yielded 57 ... RCT randomized clinical trial, RWE real world evidence, rHuEPO recombinant human erythropoietin, GH growth hormone ... proteins ≥ 200 amino acids length, including fusion proteins [etanercept] and monoclonal antibodies [adalimumab, infliximab and ... They included protein medicines used in supportive care as well as those used as therapeutic agents. The medicines contained ...
First manifestation of multiple sclerosis after immunization with the Pfizer-BioNTech COVID-19 vaccine | Journal of Neurology
SARS-CoV-2 infection was excluded on the basis of a negative PCR and absence of antibodies against the SARS-CoV-2 N protein ( ... of reactivation or new-onset demyelinating disease were reported after vaccination with Oxford-AstraZeneca COVID-19 recombinant ...
Non-invasive imaging of tau-targeted probe uptake by whole brain multi-spectral optoacoustic tomography | European Journal of...
In vitro fluorescence binding assays in recombinant fibrils. We produced tau fibrils using bacterially produced recombinant ... Synthesis of a library of oligothiophenes and their utilization as fluorescent ligands for spectral assignment of protein ... Table 1 [60, 61]. Recombinant K18 4R tau was expressed and produced by Escherichia coli as described previously [62, 63] ( ... f-j) PBB5 characterization of recombinant fibrils and staining of the human brain. (f) Transmission electron microscopy image ...
Advances in the Pathogenesis of Auto-antibody-Induced Cerebellar Synaptopathies | The Cerebellum
The presence of auto-antibodies that target synaptic machinery proteins was documented recently in immune-mediated cerebellar ... glutamate receptor interactive protein; TARP, transmembrane AMPA receptor regulatory proteins; PICK1, protein interacting with ... these actions were abolished after absorption of anti-GAD65 Ab with recombinant GAD65 [33] while anti-GAD65 Ab elicited no ... GluR delta is a postsynaptic transmembrane protein localized at the PF-PC synapse [52]. Anti-GluR delta Ab has been described ...
Do the placental barrier, parasite genotype and Toll-like receptor polymorphisms contribute to the course of primary infection...
Dobbin CA, Smith NC, Johnson AM (2002) Heat shock protein 70 is a potential virulence factor in murine toxoplasma infection via ... plus additional recombinant and atypical strains [1-4]. The three archetypic lineages were predominantly observed in North ... plus additional recombinant and atypical strains. In humans, type I and atypical strains usually associate with severe ... a novel Toxoplasma gondii dense granule protein. J Exp Med 208(1):195-212 ...
The scientific basis of rational prescribing. A guide to precision clinical pharmacology based on the WHO 6-step method |...
... influencing protein expression and protein function. Not only are "simple" molecules designed to interfere with therapeutic ... including nucleic acids or recombinant genes together with vectors (including viruses or nanoparticles), cell therapy, or ... This simple classification has evolved to a much more precise diagnosis at the level of the mutated protein that is precisely ... or protein/gene expression in biological samples such as tumor biopsies, blood, cerebrospinal fluid, or urine, in response to ...
Evaluating temperature-induced regulation of a ROSE-like RNA-thermometer for heterologous rhamnolipid production in Pseudomonas...
... protein-based thermosensors affect different cellular processes such as transcription, translation, protein stability, signal ... Integrated foam fractionation for heterologous rhamnolipid production with recombinant Pseudomonas putida in a bioreactor. AMB ... These thermosensors are part of a regulatory network, such as the production of heat shock proteins mediated by sigma factor 32 ... Changes in temperature are typically sensed as a result of conformation changes of protein structure as well as misfolded ...
Role of Nrf2/HO-1 system in development, oxidative stress response and diseases: an evolutionarily conserved mechanism |...
2a). Keap1 is a cysteine-rich protein, known to be anchored to actin cytoskeleton [5], serving as an adaptor protein for the ... Zhang X, Sato M, Sasahara M, Migita CT, Yoshida T (2004) Unique features of recombinant heme oxygenase of Drosophila ... It might also be sequestered by p62, a ubiquitin-binding protein acting as a scaffold for several protein aggregates and ... 20S protein) flanked by regulatory particles, mostly ATPases (19S proteins), which acquire substrates and direct them into the ...
Cardiovascular Injury Due to SARS-CoV-2 | SpringerLink
SARS-CoV-2 has various proteins on its surface including (M) membrane protein, (E) envelope small membrane protein and (S) ... Another type of therapeutic binds to ACE2 receptors are referred to as ACE2 soluble recombinants. ACE inhibitors have also ... Interaction of the SARS-CoV-2 spike protein with angiotensin-converting enzyme 2 (ACE2) receptors on target the cell surface ... protein [12, 13]. ACE2 is a membrane receptor that downregulates the pro-inflammatory cytokine, angiotensin II (Ang II) [12]. ...
Cryo-EM structures reveal the dynamic transformation of human alpha-2-macroglobulin working as a protease inhibitor |...
Panyutich, A., and Ganz, T. (1991). Activated α2-macroglobulin is a principal defensin-binding protein. Am J Respir Cell Mol ... folding and biochemical characterization of a high-affinity recombinant derivative. FEBS Lett 344, 242-246. ... Borth, W., and Luger, T.A. (1989). Identification of α2-macroglobulin as a cytokine binding plasma protein. J Biol Chem 264, ... Bu, G., Williams, S., Strickland, D.K., and Schwartz, A.L. (1992). Low density lipoprotein receptor-related protein/alpha 2- ...