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*  Localization of the angiopoietin receptors Tie-1 and Tie-2 on the primary cilia in the female reproductive organs - Teilmann -...
Teilmann, S. C. and Christensen, S. T. (2005), Localization of the angiopoietin receptors Tie-1 and Tie-2 on the primary cilia in the female reproductive organs. Cell Biology International, 29: 340-346. doi: 10.1016/j.cellbi.2005.03.006 ...
http://onlinelibrary.wiley.com/doi/10.1016/j.cellbi.2005.03.006/references
*  Plasma vascular endothelial growth factor, angiopoietin-2, and soluble angiopoietin receptor tie-2 in diabetic retinopathy:...
In this study, we have confirmed previous observations of increased plasma VEGF levels in diabetic patients, as well as previous observations of higher plasma VEGF levels in patients with more severe retinopathy,13 with the highest median VEGF levels among patients with pre-proliferative and proliferative retinopathy (grades 2 and 3). We also show, for the first time, that Ang-2 levels are increased in diabetics, with the highest levels among patients with grade 2 and 3 retinopathy. Furthermore, we also demonstrate that soluble tie-2 levels are lower among diabetics than controls, with no relation to the severity of retinopathy.. VEGF correlated with both Ang-2 and tie-2 among diabetics and in the whole study cohort, in keeping with these three indices being possible indices of angiogenesis. Interestingly, the correlation between Ang-2 and tie-2 was only significant in the diabetic ...
http://bjo.bmj.com/content/88/12/1543
*  Plasma angiopoietin-1, angiopoietin-2, and angiopoietin receptor tie-2 levels in congestive heart failure | JACC: Journal of...
Published data on the role of the Ang/tie-2 system in cardiovascular diseases are limited. Most of our knowledge regarding the angiopoietins has come from oncology studies where angiogenesis is a prerequisite for tumor growth and metastasis. Indeed, Ang-2 has been shown to be a marker of a poor prognosis in breast cancer and non-small cell lung cancer (4,21). In the present paper, we describe a new assay for Ang-1 and, in addition, have demonstrated (for the first time in the literature) very abnormal levels of Ang-2 and tie-2, but normal Ang-1, in CHF. Vascular endothelial growth factor was marginally raised in the patients, suggesting that Ang-2 may be more relevant to the pathophysiology of this disease. In addition, there was a significant correlation between Ang-2 and tie-2.. That Ang-1 is not raised in CHF is consistent with currently held views that its secretion is not stimulated by hypoxia, as demonstrated in animal studies ...
http://www.onlinejacc.org/content/43/3/423
*  Anti-Human Tie-1 Antibody | Human Tie-1 Antibody | Tie-1 Antibody | TIE-2 Antibody
Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE-1) is a tyrosine kinase cell-surface receptor that is expressed primarily in endothelial cells. It exhibits high homology to TEK/TIE-2 and inhibits the binding of TEK/TIE-2 to the growth factor angiopoietin-1. The extracellular domain of TIE-1 can be cleaved by multiple factors, including vascular endothelial growth factor (VEGF); this results in loss of its ability to inhibit TEK/TIE-2. In addition to its role in angiogenesis, TIE-1 is reported to have proinflammatory effects in endothelial cells. TIE-1 is also known as tyrosine-protein kinase receptor Tie-1, TIE, and JTK14.. ...
http://www.clontech.com/CA/Products/Cell_Biology_and_Epigenetics/Cancer_and_Inflammation/Tie-1
*  The Tie-2 ligand Angiopoietin-2 destabilizes quiescent endothelium through an internal autocrine loop mechanism | Journal of...
The functional consequences of angiopoietin/Tie-2 signaling have been well established through genetic loss-of-function and gain-of-function experiments (Carmeliet, 2003; Maisonpierre et al., 1997; Suri et al., 1996; Yancopoulos et al., 2000). The phenotypes of Ang-1- and Ang-2-deficient and -overexpressing mice have led to an agonistic Ang-1/Tie-2 model and an antagonistic Ang-2/Tie-2 model (Hanahan, 1997). According to these, Ang-1 activates Tie-2 and induces subsequent signal transduction promoting endothelial-cell survival, endothelial quiescence and vessel assembly. Conversely, Ang-2 is believed to act as a non-signal-transducing Tie-2 ligand that binds to endothelial Tie-2 and thereby negatively interferes with agonistic Ang-1/Tie-2 functions. As such, it does not exert functions of its own but rather acts as a facilitator of other vascular cytokines. The net outcome of Ang-2 functions ...
http://jcs.biologists.org/content/118/4/771
*  Endothelial receptor tyrosine kinases activate the STAT signaling pathway: mutant Tie-2 causing venous malformations signals a...
Endothelial receptor tyrosine kinases (RTKs) and their signaling mechanisms are of interest because they may control tumor angiogenesis and thereby tumor growth. In this report we have examined activation of the signal transducers and activators of transcription (STATs) by the three known vascular endothelial growth factor receptors (VEGFR1-3), as well as by the endothelial Tie-1 and -2 receptors. We also studied signaling by the R849W mutant of Tie-2 (MTie-2), which has been shown to cause venous malformations. When overexpressed in 293T cells, MTie-2 activated STAT1 while the other endothelial RTKs failed to do so. In contrast, the three VEGFRs were strong activators of STAT3 and STAT5, suggesting that they activate only a specific subset of these signal transducers. STAT3 and STAT5 were also activated by Tie-2 and, more so, by MTie-2. Tyrosine ...
https://dial.uclouvain.be/pr/boreal/object/boreal:130908
*  ActoFactor™ Recombinant Mouse soluble TIE-1/Fc Chimera | Creative Bioarray
TIE-1 and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)- like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Murine TIE-1 cDNA encodes a 1134 amino acid (aa) precursor protein with a 22 aa putative signal peptide, a 733 aa extracellular domain and a 354 aa cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1. Recombinant murine soluble TIE-1 was fused with the Fc part of ...
https://www.creative-bioarray.com/ActoFactor-Recombinant-Mouse-soluble-TIE-1-Fc-Chimera-CSC-CTK0790-item-4116.htm
*  TEK gene - Genetics Home Reference
The TEK gene (also called the TIE2 gene) provides instructions for making a protein called TEK receptor tyrosine kinase. The TEK receptor tyrosine kinase (or TEK receptor) is active (expressed) mainly in endothelial cells, which line the walls of blood vessels. When the TEK receptor is activated, it triggers a series of chemical signals that facilitates communication between endothelial cells and smooth muscle cells. Layers of smooth muscle cells surround layers of endothelial cells lining the walls of blood vessels. Communication between these two cell types is necessary to direct blood vessel formation (angiogenesis) and ensure the structure and integrity of blood vessels.. The TEK receptor is also found in bone marrow, where it is expressed in blood-forming cells called hematopoietic stem cells. The role of the TEK receptor in hematopoietic stem cells is unknown. Researchers speculate ...
https://ghr.nlm.nih.gov/gene/TEK
*  RCSB PDB for 1HZ8
1HZ8: NMR structure and backbone dynamics of a concatemer of epidermal growth factor homology modules of the human low-density lipoprotein receptor.
http://www.rcsb.org/pdb/explore/biologyAndChemistry.do?structureId=1HZ8
*  Plus it
4844 Tie-2 stabilises pericyte/endothelial interactions during angiogenesis and is over expressed on tumor endothelium. A vaccine that targets endothelium over-expressing Tie-2 may result in vessel damage and stimulate an inflammatory cascade resulting in disease regression. To validate the use of Tie-2 as a target, human Tie-2 was used as a xenogenic vaccine in mice. Preliminary studies suggest that immunisation of balb/c mice by gene gun with DNA from the extracellular region of Tie-2 resulted in cessation of tumor growth. To determine if we could design a syngeneic vaccine that would work in both mice and humans a conserved region of Tie- 2 was cloned. HLA-A*0201 transgenic mice were immunised with this construct to determine if a repertoire of HLA-A*0201 T cells exist that recognise Tie-2. Within the Tie-2 region an HLA-A*0201 epitope was identified that is identical ...
http://cancerres.aacrjournals.org/content/64/7_Supplement/1119.3
*  JCI - Tie1 controls angiopoietin function in vascular remodeling and inflammation
The angiopoietin/Tie (ANG/Tie) receptor system controls developmental and tumor angiogenesis, inflammatory vascular remodeling, and vessel leakage. ANG1 is a Tie2 agonist that promotes vascular stabilization in inflammation and sepsis, whereas ANG2 is a context-dependent Tie2 agonist or antagonist. A limited understanding of ANG signaling mechanisms and the orphan receptor Tie1 has hindered development of ANG/Tie-targeted therapeutics. Here, we determined that both ANG1 and ANG2 binding to Tie2 increases Tie1-Tie2 interactions in a β1 integrin-dependent manner and that Tie1 regulates ANG-induced Tie2 trafficking in endothelial cells. Endothelial Tie1 was essential for the agonist activity of ANG1 and autocrine ANG2. Deletion of endothelial ...
https://jci.org/articles/view/84923/figure/1
*  VMCM - Genetics Home Reference
Mutations in the TEK gene (also called the TIE2 gene) cause VMCM. The TEK gene provides instructions for making a protein called TEK receptor tyrosine kinase. This receptor protein triggers chemical signals needed for forming blood vessels (angiogenesis) and maintaining their structure. This signaling process facilitates communication between two types of cells within the walls of blood vessels, endothelial cells and smooth muscle cells. Communication between these two cell types is necessary to direct angiogenesis and ensure the structure and integrity of blood vessels.. TEK gene mutations that cause VMCM result in a TEK receptor that is always turned on (overactive). An overactive TEK receptor is thought to disrupt the communication between endothelial cells and smooth muscle cells. It is unclear how a lack of communication between these cells causes venous malformations. These abnormal blood vessels show a deficiency of ...
https://ghr.nlm.nih.gov/condition/multiple-cutaneous-and-mucosal-venous-malformations
*  Distal angiogenesis: a new concept for lung vascular morphogenesis | Lung Cellular and Molecular Physiology
We describe the development of the pulmonary vasculature in the mouse from the first morphological sign of lung development (E9.5) until early pseudoglandular stage (E13.5) through the analysis of whole mount X-gal-stained fetal lungs of Tie2-LacZ transgenic mice. The transgenic strain expresses the bacterial lacZ gene under the control of the Tie2 promoter, and cells that convert the X-gal substrate are positive for the angiopoietin receptor Tie2 (31). We expanded our analysis of the pulmonary vasculature using immunohistochemistry on serial sections of wild-type mice with two distinct endothelial-specific cell markers, PECAM-1 and Fli-1. Furthermore, the embryos have been isolated and processed without disrupting the circulation to leave the vascular tone and integrity intact. This procedure prevents the collapse of vessels and the putative creation of artifacts in the sections. Therefore, we were able to follow individual structures ...
http://ajplung.physiology.org/content/288/1/L141.long
*  PHD2 regulates arteriogenic macrophages through TIE2 signalling - Hamm - 2013 - EMBO Molecular Medicine - Wiley Online Library
This study identifies a basic biological role for PHD2 in the control of a specific proarteriogenic macrophage phenotype that impinges on TIE2 signalling. Overall, the mechanisms described in this study may represent an indirect modality by which PHD2 modulates oxygen delivery through the regulation of vessel morphogenesis.. The proarteriogenic tissue macrophages identified here are highly reminiscent of the M2-like, proangiogenic TEMs found in tumours, developing organs and regenerating tissues (Capobianco et al, 2011; De Palma et al, 2005; Fantin et al, 2010; Mazzieri et al, 2011; Pucci et al, 2009). Despite the well-characterized proangiogenic functions of TEMs, no studies have yet been undertaken to rigorously assess the functional relevance of the ANG receptor TIE2 in macrophages in the context of ischaemia. We have previously shown that, similar to tumour-associated TEMs (Pucci et al, 2009), Phd2+/− macrophages do not upregulate either Vegf or ...
http://onlinelibrary.wiley.com/doi/10.1002/emmm.201302695/full?globalMessage=0
*  The Definition of Cell Type | Circulation Research
What do we mean by "cell type?" Two articles in this issue of Circulation Research address this question in different ways. The first study, by Dube et al,1 uses very well-defined tools to define endothelial differentiation at the level of transcriptional control. The second study, by Kowal et al,2 looks for novel genes that distinguish two cell types. These studies, taken together, illustrate a major change in how we define cell type, a change that is about to be accelerated by the power of systematic genomics.. Dube et al1 use in vitro systems to explore the promoter structure for the endothelial cell-specific receptor tyrosine kinase Tie2. Tie2 is a receptor for both angiopoetin-1 and angiopoetin-2. Like vascular endothelial growth factor, angiopoetin-1 is essential for normal vascular development whereas angiopoetin-2 is a naturally occurring antagonist for angiotensin I and Tie2. Thus, regulation of expression of the ...
http://circres.ahajournals.org/content/84/10/1234
*  Tie-2 antibody
Does anybody know of a Tie-2 antibody that work for FACS?. I've tried 3 antibodies to the extra cellular part of the receptor (from Santa Cruz, from R&D and from RDI) without success although they all work in westerns. Thanks, Juan Oliver ...
https://lists.purdue.edu/pipermail/cytometry/2001-March/019056.html
*  Abstract 482: VEGF Induces MMP-dependent Tie2 Cleavage via PI3K-AKT | Circulation
OBJECTIVES: Tie2 and its ligands, the angiopoietins (Ang), are required for embryonic and postnatal angiogenesis. Previous studies have demonstrated that Tie2 is proteolytically cleaved from endothelial cells and produces a 75 kDa soluble receptor fragment (sTie2), however, the signaling mechanisms and effector molecules responsible for the shedding phenomenon are unknown. We investigated mechanisms responsible for Tie2 shedding.. MATERIALS: Human umbilical vein endothelial cells (HUVECs), NIH-3T3, or HEK293 cells exogenously expressing full-length Tie2 were serum starved for varying timepoints. The conditioned media (CM) were then analyzed for the presence of Tie2 by ELISA or westernblot using a Tie2-specific antibody recognizing the extracellular domain. VEGF-A165 (R&D systems) was used for experimentation. Pharmacologic inhibitors of p38 MAPK, Akt, and PI3K were also ...
http://circ.ahajournals.org/content/116/Suppl_16/II_82.2
*  Plus it
Although therapeutic strategies targeting TAMs, through the CSF-1/CSF-1R pathway, are currently being evaluated clinically, further translational opportunities must be developed on the basis of emerging knowledge. In particular, we need to better decipher the molecular mechanisms controlling TAM activity in vivo; the heterogeneity of human TAM subsets, e.g., their origin as CD14+, CD16+, or Tie-2 monocytes; TAM function and phenotypes, e.g., the relationship between the Tie-2+ TAM subset and the M2-immunosuppressive type; and the basis for TAM plasticity during progression in terms of their microenvironment reprogramming potential.. Translational challenges relate in part to the fact that present knowledge about TAM is based mainly on preclinical studies conducted in mouse models of cancer, mainly those employing transplanted tumors or multifocal spontaneous tumors. In-depth analyses of TAM subsets in human subjects are needed to validate the utility of the ...
http://cancerres.aacrjournals.org/content/76/22/6439
*  Tie-2 Antibody [DyLight 488] (NBP1-69753G): Novus Biologicals
Rabbit Polyclonal Anti-Tie-2 Antibody [DyLight 488]. Validated: WB, IHC, IHC-P. Tested Reactivity: Human, Mouse. 100% Guaranteed.
https://www.novusbio.com/products/tie-2-antibody_nbp1-69753g
*  the joy of sox: G4: Cleveland 3, Red Sox 1
GS IP ERA OPS 2006 with Tek 22 135.0 4.93 .752 w/out Tek 12 69.2 5.17 .794 2007 with Tek 29 192.2 3.32 .666 w/out Tek 1 8.0 2.25 .577 2008 with Tek 27 174.1 4.03 .700 w/out Tek 0 2009 with Tek 27 184.2 3.17 .625 w/out Tek 6 27.2 8.46 1.033* 2010 with Tek 6 36.1 7.18 .851 w/out Tek 15 91.1 5.22 . ...
http://joyofsox.blogspot.com/2011/04/g4-red-sox-at-cleveland-7-pm.html?showComment=1302045572624
*  the joy of sox: G4: Cleveland 3, Red Sox 1
GS IP ERA OPS 2006 with Tek 22 135.0 4.93 .752 w/out Tek 12 69.2 5.17 .794 2007 with Tek 29 192.2 3.32 .666 w/out Tek 1 8.0 2.25 .577 2008 with Tek 27 174.1 4.03 .700 w/out Tek 0 2009 with Tek 27 184.2 3.17 .625 w/out Tek 6 27.2 8.46 1.033* 2010 with Tek 6 36.1 7.18 .851 w/out Tek 15 91.1 5.22 . ...
http://joyofsox.blogspot.com/2011/04/g4-red-sox-at-cleveland-7-pm.html?showComment=1302054434918
*  Poema Odisea-analizë letrare apo kritike letrare | Rapitful Shqip
Poema Odisea-analizë letrare apo kritike letrare,kritika letrare,odisea analize letrare,Homeri Odisea analize letrare,kritika letrare per vepren Odisea,Odise Analize letrare,Subjekti tek Odisea,Tema tek vepra Odisea,simboli tek vepra Odisea,Poema odisea analize letrare,Odisea Subjekti,Odisea Tema,Odisea shqip,Odisea kritika,personazhet tek vepra odisea,odisea personazhet,cilet jane personazhet tek vepra odisea,odisea personazhet shqip
http://rapitful.blogspot.com/2012/05/poema-odisea-analize-letrare-apo.html
*  Gentaur Molecular :Sakura \ Cyto Tek® 6 mL Fluid Chamber; 12 cs \ 4331
Gentaur molecular products has all kinds of products like :search , Sakura \ Cyto_Tek® 6 mL Fluid Chamber; 12_cs \ 4331 for more molecular products just contact us
http://www.antibody-antibodies.com/product_det.php?id=1015688&supplier=search&name=Cyto_Tek%C2%AE%206%20mL%20Fluid%20Chamber%3B%2012_cs
*  Forum opiekunek osób starszych • Zobacz wątek - Hula-hop
najważniejsze żeby się regularnie ruszać a czy to z hula hop czy bieganie to już każdego indywidualna sprawa, ja pojechałam na [link usunięte ze względu na reklamowy charakter // Admin]warsztaty muzyczne i chyba na jakieś tańce się zapiszę, bo poczułam rytm i może wreszcie naucze się ...
http://forumopieki.pl/viewtopic.php?f=25&t=1239&sid=f60a3f8379149874c29e81c9763adf50&start=10
*  autoreferat Bartosz Mucha - PDF
autoreferat Bartosz Mucha POCZĄTEK Studia z projektowania graficznego wybrałem świadomie, zafascynowany głównie plakatem i jego stroną narracyjną, typowymi dla polskich projektów rebusami oraz ukrytymi
http://docplayer.pl/3433461-Autoreferat-bartosz-mucha.html