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*  Patente US5538871 - In situ polymerase chain reaction - Google Patentes
... can be achieved by changing the way that the enzymatic reaction is started. Reaction initiation is delayed until the start of ... or by adding to the PCR reagents a single-stranded DNA binding protein which blocks reaction at temperatures below about 50 C. ... Improvements to the in situ polymerase chain reaction (PCR), a process of in vitro enzymatic amplification of specific nucleic ... Real-time detection of rolling circle amplification products. US20040126789 *. 5 Sep 2003. 1 Jul 2004. Invitrogen Corporation. ...
http://www.google.es/patents/US5538871
*  DNA-directed RNA polymerase I subunit RPA12
The results of human miRNA array and real-time PCR showed that ZNRD1 could significantly up-regulate the expression of miR-214 ... Verapamil inhibits the reaction [ZNRD1 protein results in decreased susceptibility to Doxorubicin] 1 ... RNA Polymerase I Chain Elongation 55 RNA Polymerase I Promoter Clearance 74 ... Component of RNA polymerase I which synthesizes ribosomal RNA precursors. This gene encodes a DNA-directed RNA polymerase I ...
https://pharos.nih.gov/idg/targets/Q9P1U0
*  Real-time polymerase chain reaction - Wikipedia
A real-time polymerase chain reaction (Real-Time PCR), also known as quantitative polymerase chain reaction (qPCR), is a ... The acronym "RT-PCR" commonly denotes reverse transcription polymerase chain reaction and not real-time PCR, but not all ... Bustin, SA (2000). "Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays". J ... in real-time, and not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR ...
https://en.wikipedia.org/wiki/Real-time_polymerase_chain_reaction
*  07 | Real Time Polymerase Chain Reaction | Polymerase Chain Reaction
Use of the polymerase chain reaction for the specific and direct detection of Clostridium difficile in human feces. Rev. Infect ... Differentiation of bifidobacteria by use of pulsed-field gel electrophoresis and polymerase chain reaction. Int. J. Food ... Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 245: 154-610. ... The use of Real-Time PCR equipment, which is discussed later, makes it possible to identify bacterial species from the melting ...
https://www.scribd.com/document/188317783/07
*  Rapid Determination of Sexually Transmitted Infections by Real-time Polymerase Chain Reaction Using Microchip Analyzer
Development of non expensive and time-saving techniques based on the polymerase chain reaction (PCR) is of great importance for ... "Rapid Determination of Sexually Transmitted Infections by Real-time Polymerase Chain Reaction Using Microchip Analyzer," ... "Microchip analytic system for multiplex analysis by real-time polymerase chain reaction with reagents immobilized in ... Development of non expensive and time-saving techniques based on the polymerase chain reaction (PCR) is of great importance for ...
https://www.scirp.org/journal/PaperInformation.aspx?PaperID=26716
*  Quadruplex real-time polymerase chain reaction assay for molecular diagnosis of Y-chromosomal microdeletions.
INTERVENTION(S): We developed a quadruplex real-time polymerase chain reaction (PCR) assay and evaluated its performance in ... RESULT(S): The limit of detection of quadruplex real-time PCR assay was 100 pg genomic DNA. The method attained 100% analytic ... Therefore, it is strongly suggested that such quadruplex real-time PCR assay can be readily applied as clinical routine in the ...
http://www.biomedsearch.com/nih/Quadruplex-real-time-polymerase-chain/22270555.html
*  Differential Diagnosis of Entamoeba spp. in Clinical Stool Samples Using SYBR Green Real-Time Polymerase Chain Reaction
in Clinical Stool Samples Using SYBR Green Real-Time Polymerase Chain Reaction. Thiago dos Santos Gomes,1 Mariana Coimbra ... in Clinical Stool Samples Using SYBR Green Real-Time Polymerase Chain Reaction," The Scientific World Journal, vol. 2014, ...
https://www.hindawi.com/journals/tswj/2014/645084/cta/
*  Development and Validation of a Pneumocystis jirovecii Real-time Polymerase Chain Reaction Assay for Diagnosis of Pneumocystis...
Development and Validation of a Pneumocystis jirovecii Real-time Polymerase Chain Reaction Assay for Diagnosis of Pneumocystis ... "Development and Validation of a Pneumocystis jirovecii Real-time Polymerase Chain Reaction Assay for Diagnosis of Pneumocystis ...
https://www.hindawi.com/journals/cjidmm/2015/138787/cta/
*  The new real-time polymerase chain reaction detection system at NIOM | NIOM
The real-time polymerase chain reaction (PCR) detection system opens up new possibilities for research at NIOM. The instrument ... The new real-time polymerase chain reaction detection system at NIOM. Bergitte Olderbø , November 29th 2016 ... while real-time PCR detects DNA by fluorescence at each amplification cycle as it occurs (real time). This enables measurement ... The real-time PCR detection system has the possibility to discriminate up to five different targets in one reaction well. The ...
https://niom.no/the-new-real-time-polymerase-chain-reaction-detection-system-at-niom/
*  Identification of Common Bacterial Pathogens Causing Meningitis in Culture-Negative Cerebrospinal Fluid Samples Using Real-Time...
Pathogens Causing Meningitis in Culture-Negative Cerebrospinal Fluid Samples Using Real-Time Polymerase Chain Reaction. Walaa ... culture-negative CSF samples from suspected cases of bacterial meningitis were examined by real-time polymerase chain reaction ... samples were negative by real-time PCR for all tested organisms. Conclusion. The use of molecular techniques as real-time PCR ... Positive real-time PCR results for Streptococcus pneumoniae were detected in 36 (90%) of culture-negative CSF samples while no ...
https://www.hindawi.com/journals/ijmicro/2016/4197187/abs/
*  Use of a real-time polymerase chain reaction thermocycler to study bacterial cell permeabilization by antimicrobial peptides. |...
Use of a real-time polymerase chain reaction thermocycler to study bacterial cell permeabilization by antimicrobial peptides.. ... Using a simple protocol based on the use of a standard real-time thermocycler, we confirmed that the actions of the ...
https://www.sigmaaldrich.com/catalog/papers/18656439
*  Quantitative Real-Time Polymerase Chain Reaction Measurement of HLA-DRA Gene Expression in Whole Blood Is Highly Reproducible...
... measured by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR). Aims The aims of this study were: 1. to validate the ... PLOS ONE promises fair, rigorous peer review, broad scope, and wide readership - a perfect fit for your research every time. ...
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154690
*  The American Journal of Tropical Medicine and Hygiene | Real-Time Polymerase Chain Reaction Assay on Bronchoalveolar Lavage: An...
In the present work, we retrospectively tested an in-house real-time polymerase chain reaction for Schistosoma (currently ... Real-Time Polymerase Chain Reaction Assay on Bronchoalveolar Lavage: An Alternative Method for Diagnosing Chronic Pulmonary ... Real-Time Polymerase Chain Reaction Assay on Bronchoalveolar Lavage: An Alternative Method for Diagnosing Chronic Pulmonary ... In the present work, we retrospectively tested an in-house real-time polymerase chain reaction for Schistosoma (currently ...
http://www.ajtmh.org/content/journals/10.4269/ajtmh.17-0499
*  Development and evaluation of a real-time polymerase chain reaction for fast diagnosis of sporotrichosis caused by Sporothrix...
Real-time Polymerase Chain Reaction. Methods used for detecting the amplified DNA products from the polymerase chain reaction ... Polymerase Chain Reaction (PCR) » Research » Development and evaluation of a real-time polymerase chain reaction for fast ... Polymerase Chain Reaction (PCR) PCR (Polymerase Chain Reaction) uses the ability of DNA polymerase (enzymes that create DNA ... Multiplex Polymerase Chain Reaction. Methods for using more than one primer set in a polymerase chain reaction to amplify more ...
https://www.bioportfolio.com/resources/pmarticle/2330452/Development-and-evaluation-of-a-real-time-polymerase-chain-reaction-for-fast.html
*  The American Journal of Tropical Medicine and Hygiene | QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION FOR MALARIA DIAGNOSIS...
The use of quantitative real-time polymerase chain reaction (PCR), carried out in real-time during the clinical trials of ... The use of quantitative real-time polymerase chain reaction (PCR), carried out in real-time during the clinical trials of ... f QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION FOR MALARIA DIAGNOSIS AND ITS USE IN MALARIA VACCINE CLINICAL TRIALS * ... QUANTITATIVE REAL-TIME POLYMERASE CHAIN REACTION FOR MALARIA DIAGNOSIS AND ITS USE IN MALARIA VACCINE CLINICAL TRIALS ...
http://www.ajtmh.org/content/journals/10.4269/ajtmh.2005.73.191
*  The American Journal of Tropical Medicine and Hygiene | EVALUATION OF A REAL-TIME POLYMERASE CHAIN REACTION ASSAY FOR THE...
Within the limitations of initial equipment costs, this real-time PCR assay is a rapid, accurate, and efficient method for the ... assay can be completed in one hour and has the potential to detect and identify four species of Plasmodium in a single reaction ... We compared the diagnosis of malaria in 297 patients from Thailand by a real-time polymerase chain reaction (PCR) assay using ... f EVALUATION OF A REAL-TIME POLYMERASE CHAIN REACTION ASSAY FOR THE DIAGNOSIS OF MALARIA IN PATIENTS FROM THAILAND * HEATHER ...
http://www.ajtmh.org/content/journals/10.4269/ajtmh.2005.73.850
*  Development and Evaluation of a Multiplex Quantitative Real-Time Polymerase Chain Reaction for Hookworm Species in Human Stool ...
The present study aims to evaluate a multiplex real-time quantitative PCR (qPCR) assay to determine the prevalence and egg ... of all three hookworm species and compare this with standard microscopy and published genus-based conventional and real-time ... oa Development and Evaluation of a Multiplex Quantitative Real-Time Polymerase Chain Reaction for Hookworm Species in Human ... Development and Evaluation of a Multiplex Quantitative Real-Time Polymerase Chain Reaction for Hookworm Species in Human Stool ...
http://www.ajtmh.org/content/journals/10.4269/ajtmh.18-0276
*  Performance of Real-Time Polymerase Chain Reaction Assays for the Detection of 20 Gastrointestinal Parasites in Clinical...
This study aims to compare the performance of real-time polymerase chain reaction (PCR) assays to microscopic examination for ... Real-time PCR was positive in 72 of 98 samples (73.5%), whereas microscopic examination was positive in 37 (37.7%) samples (P ... Positive frequency and the sensitivity of each real-time PCR assay were compared with conventional microscopic examination. ... Real-time PCR appeared to be superior to microscopic examination for the detection of parasites in stool samples. This assay ...
http://www.ajtmh.org/content/journals/10.4269/ajtmh.16-0781
*  The American Journal of Tropical Medicine and Hygiene | Clinical Usefulness of Real-Time Polymerase Chain Reaction for the...
Here, we aimed to examine the clinical usefulness of polymerase chain reaction (PCR) using tissue specimens other than blood ... The median V. vulnificus DNA load of 14 patients in real-time PCR analysis of serum at the time of admission was 638.5 copies/ ... the median value by real-time PCR using the first tissue specimen at the time of admission was 16,650 copies/mL tissue fluid ( ... A retrospective study was conducted with patients who underwent real-time PCR of toxR in both blood and skin tissues, including ...
http://www.ajtmh.org/content/journals/10.4269/ajtmh.16-0512
*  Development and Accuracy of Quantitative Real-Time Polymerase Chain Reaction Assays for Detection and Quantification of...
Quantitative real-time polymerase chain reaction (qPCR) assays that detect the ETEC toxin genes eltA, sta1, and sta2 in ... Quantitative real-time polymerase chain reaction (qPCR) assays that detect the ETEC toxin genes eltA, sta1, and sta2 in ... f Development and Accuracy of Quantitative Real-Time Polymerase Chain Reaction Assays for Detection and Quantification of ... Development and Accuracy of Quantitative Real-Time Polymerase Chain Reaction Assays for Detection and Quantification of ...
http://www.ajtmh.org/content/journals/10.4269/ajtmh.13-0383
*  DIGITAL.CSIC: Real-Time Polymerase Chain Reaction for Quantitative Detection of Histamine-Producing Bacteria: Use in Cheese...
Real-Time Polymerase Chain Reaction for Quantitative Detection of Histamine-Producing Bacteria: Use in Cheese Production. ... The aim of the present work was to develop a real-time quantitative PCR assay for the direct detection and quantification of ... Chromatographic methods (HPLC) verified the capacity of real-time quantitative PCR to correctly quantify histamine accumulation ... The results show the proposed procedure to be a rapid (total processing time , 2 h), specific and highly sensitive technique ...
https://digital.csic.es/handle/10261/5718
*  Development of Multiplex Real-Time Polymerase Chain Reaction for Detection of Entamoeba histolytica, Entamoeba dispar, and...
Multiplex real-time polymerase chain reaction (PCR) was developed for differential detection of Entamoeba histolytica, ... Multiplex real-time polymerase chain reaction (PCR) was developed for differential detection of Entamoeba histolytica, ... Development of Multiplex Real-Time Polymerase Chain Reaction for Detection of Entamoeba histolytica, Entamoeba dispar, and ... f Development of Multiplex Real-Time Polymerase Chain Reaction for Detection of Entamoeba histolytica, Entamoeba dispar, and ...
http://www.ajtmh.org/content/journals/10.4269/ajtmh.2010.10-0050
*  Detection of viable but nonculturable Vibrio parahaemolyticus induced by prolonged cold-starvation using propidium monoazide...
... staining and real-time polymerase chain reaction (qPCR) with propidium monoazide (PMA), and its resuscitative ability was ... Polymerase Chain Reaction (PCR) PCR (Polymerase Chain Reaction) uses the ability of DNA polymerase (enzymes that create DNA ... Vibrio parahaemolyticus induced by prolonged cold-starvation using propidium monoazide real-time polymerase chain reaction.. 08 ... Vibrio parahaemolyticus induced by prolonged cold-starvation using propidium monoazide real-time polymerase chain reaction. ...
https://www.bioportfolio.com/resources/pmarticle/2332931/Detection-of-viable-but-nonculturable-Vibrio-parahaemolyticus-induced-by-prolonged-cold-starvation.html
*  Methodologic and biological variability of quantitative real-time polymerase chain reaction analysis of Bcr-Abl expression in...
Methodologic and biological variability of quantitative real-time polymerase chain reaction analysis of Bcr-Abl expression in ... Methodologic and biological variability of quantitative real-time polymerase chain reaction analysis of Bcr-Abl expression in ... Methodologic and biological variability of quantitative real-time polymerase chain reaction analysis of Bcr-Abl expression in ...
http://www.haematologica.org/content/88/9/1074
*  Urine real-time polymerase chain reaction detection for children virus pneumonia with acute human cytomegalovirus infection |...
Real-time polymerase chain reaction (PCR) and a chemiluminescent assay were used to detect HCMV DNA copy number, the pp65 gene ... Our study shows a novel method for investigating acute pulmonary HCMV infection in children by using real-time PCR and non- ... Real-time polymerase chain reaction (PCR) and a chemiluminescent assay were used to detect HCMV DNA copy number, the pp65 gene ... Urine real-time polymerase chain reaction detection for children virus pneumonia with acute human cytomegalovirus infection. ...
https://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-14-245