Quantification of Myotrophin From Spontaneously Hypertensive and Normal Rat Hearts | Circulation Research
Solid-Phase Radioimmunoassay. Both natural myotrophin and MAP were used as antigens to evaluate the binding capacities of the ... Solid-phase radioimmunoassays and Western blot analyses were used for the characterization of the antibody formed against MAP ... Competitive binding radioimmunoassay techniques were used for the construction of a Scatchard plot to determine the affinity ... The antibody was characterized by solid-phase radioimmunoassays and Western blot analysis. To define the specificity of the ...http://circres.ahajournals.org/content/76/6/1020
Radioimmunoassay of Prostaglandin A | Circulation Research
Radioimmunoassay of Prostaglandin A. Intrarenal PGA2 as a Factor Mediating Saline-Induced Natriuresis. AHMAD A. ATTALLAH, JAMES ... A highly specific, sensitive (25 pg) radioimmunoassay for prostaglandin A is described; the assay is characterized by high ...http://circres.ahajournals.org/content/33/6/696
Induction of Monocyte Chemoattractant Protein-1 Synthesis in Human Monocytes During Transendothelial Migration In Vitro |...
Radioimmunoassay for MCP-1. Competitive radioimmunoassay (RIA) for the quantification of MCP-1 was developed by use of 125I- ... MCP-1 concentration in the conditioned medium was measured by radioimmunoassay. A, Unstimulated HUVECs. B, IL-1β (25 U/mL)- ... MCP-1 concentrations in the conditioned media were measured by radioimmunoassay. Values represent mean±SEM for duplicate ... and MCP-1 concentration in the conditioned medium was measured by radioimmunoassay. Values represent mean±SEM for duplicate ...http://circres.ahajournals.org/content/76/5/750
Expression of Proto-oncogenes and Gene Mutation of Sarcomeric Proteins in Patients With Hypertrophic Cardiomyopathy |...
Radioimmunoassay of c-Myc protein. Jpn J Cancer Res. 1986;77:615-619. ...http://circres.ahajournals.org/content/83/6/594
Effect of Tetrahydrobiopterin on Endothelial Function in Canine Middle Cerebral Arteries | Circulation Research
A cGMP radioimmunoassay kit (Amersham) was used to perform the measurements.. The role of calcium in production of cGMP was ... A radioimmunoassay technique was used to determine the levels of cGMP. After incubation for 24 hours, indomethacin (10−5 mol/L ... Production of cGMP was measured by radioimmunoassay. Incubation with sepiapterin markedly increased intracellular ...http://circres.ahajournals.org/content/79/2/336
Angiotensin-converting enzyme activity and its modulation by oxygen tension in the guinea pig fetal-placental unit. |...
Peptide concentrations were measured by radioimmunoassay (RIA). Using BK as substrate, we found that placental and fetal ACE ...http://circres.ahajournals.org/content/48/2/286
Endothelin-1 and Angiotensin II Receptors in Cells From Rat Hypertrophied Heart | Circulation Research
Radioimmunoassays: ANP-(1-98), ANP-(99-126), BNP, ET-1, Ang II, and Plasma Renin Activity Plasma and cardiac peptide ... Interference of eluates from octadecyl cartridges with an angiotensin II radioimmunoassay. Peptides. 1986;7:365-367. ... concentrations and PRA were measured by radioimmunoassay. Blood was collected in ice-chilled tubes containing EDTA and ...http://circres.ahajournals.org/content/78/2/302
Regulation of Aldosterone Biosynthesis by Adrenal Renin Is Mediated Through AT1 Receptors in Renin Transgenic Rats |...
The radioimmunoassay sensitivity is 10 pg per tube. The intra-assay and interassay variabilities of this method were 3.7% and ... Corticosterone was determined by a radioimmunoassay kit (Eurogenetics). The sensitivity of this assay is 25 ng/mL. The ... Aldosterone was measured by a direct radioimmunoassay kit (Diagnostic Product Corp). The sensitivity of this assay is 16 pg/mL ... generated during the incubation step was quantified by radioimmunoassay and expressed as nanograms Ang I per milliliter per ...http://circres.ahajournals.org/content/77/1/73
Angiotensin-Converting Enzyme II in the Heart and the Kidney | Circulation Research
Radioimmunoassay for immunoreactive [des-Leu10]-angiotensin I. Peptides (Elmsford). 1989; 10: 489-492. ... An alternative strategy for the radioimmunoassay of angiotensin peptides using amino-terminal-directed antisera: measurement of ...http://circres.ahajournals.org/content/98/4/463
Identification of Epoxyeicosatrienoic Acids as Endothelium-Derived Hyperpolarizing Factors | Circulation Research
The samples were stored at −80°C until assayed by radioimmunoassay. Before assay, the tissues were homogenized with a ... cAMP and cGMP were measured by radioimmunoassay as previously described.34 35 ...http://circres.ahajournals.org/content/78/3/415
Regional Expression of Natriuretic Peptide Receptors During the Formation of Arterial Neointima in the Rabbit | Circulation...
Kits for radioimmunoassay of cAMP and cGMP were also from Amersham. NTB3 photographic emulsion was from Kodak Ltd. Hypnorm was ... Samples were then centrifuged at 4000g for 10 minutes at 4°C. cGMP was measured in supernatants by radioimmunoassay.19 26 All ... and supernatant cAMP was then measured by radioimmunoassay.20 All measurements from one rabbit were performed in a single assay ...http://circres.ahajournals.org/content/77/5/906
Critical Role of AT1 Receptor Expression After Ischemia/Reperfusion in Isolated Rat Hearts | Circulation Research
Samples were analyzed by double-antibody Ang II radioimmunoassay (RK-A22, Alpco), as described earlier.24 The assay is ...http://circres.ahajournals.org/content/83/5/552
Low Increase in cGMP Induced by Organic Nitrates and Nitrovasodilators Improves Contractile Response of Rat Ventricular...
... and directly used for determination of cGMP by radioimmunoassay using a 125I-labeled antigen. The recovery rate of protein and ... and directly used for determination of cGMP by radioimmunoassay using a 125I-labeled antigen. All experiments were performed ... 20 The supernatant was directly used for determination of cAMP by radioimmunoassay using a tritium-labeled antigen. ...http://circres.ahajournals.org/content/78/1/91
Endothelium-dependent contractions in rabbit pulmonary artery are mediated by thromboxane A2. | Circulation Research
Using specific radioimmunoassays, AA and MeCH stimulated 6-keto-PGF1 alpha and TXB2 release. Indomethacin blocked the ...http://circres.ahajournals.org/content/72/5/1023
Chronic Production of Angiotensin IV in the Brain Leads to Hypertension That Is Reversible With an Angiotensin II AT1 Receptor...
Test bleeds (8 mL) were collected between the injections and evaluated for positive immune response by radioimmunoassay (RIA). ... Radio- immunoassay for total brain and plasma content of modified Ang IV peptide in transgenic mice from lines GFAP-10.2 and ... alcohol extracts from line GFAP10.2 and control were fractionated by HPLC and eluted peptides were detected by radioimmunoassay ...http://circres.ahajournals.org/content/94/11/1451
Table of Contents - August 01, 1979, 45 (2) | Circulation Research
Specific antibody to hog renal renin and its application to the direct radioimmunoassay of renin in various organs. S Hirose, R ...http://circres.ahajournals.org/content/45/2
Nongenomic Effects of Aldosterone on Intracellular Ca2+ in Vascular Smooth Muscle Cells | Circulation Research
The development and application of direct radioimmunoassay for plasma aldosterone using 131-I-labelled ligand-comparison of ...http://circres.ahajournals.org/content/76/6/973
Protective Role of Bradykinin in Cardiac Anaphylaxis | Circulation Research
Briefly, samples were reconstituted with radioimmunoassay buffer and incubated with rabbit anti-bradykinin serum in ... and for 5 minutes after antigen and assayed for kinins by radioimmunoassay. Bars are mean±SEM (n=3). *P,.05 vs control by t ...http://circres.ahajournals.org/content/76/3/434
Dynorphin B Is an Agonist of Nuclear Opioid Receptors Coupling Nuclear Protein Kinase C Activation to the Transcription of...
However, radioimmunoassay of immunoreactive dynorphin B in ES cells revealed that, though most of the peptide was targeted for ... Immunoreactive dynorphin B-like material was previously detected by a radioimmunoassay procedure in acetic acid extracts from ... Confirming the results from radioimmunoassay analysis, immunostaining of dynorphin B-like material was faintly detected in ...http://circres.ahajournals.org/content/92/6/623
Antisense to Thyrotropin Releasing Hormone Receptor Reduces Arterial Blood Pressure in Spontaneously Hypertensive Rats |...
Localisation of thyrotropin releasing hormone in the spinal cord of the rat by immunohistochemistry and radioimmunoassay. Proc ...http://circres.ahajournals.org/content/77/4/679
Plasma renin activity during exercise in the dog. | Circulation Research
PRA was measured by radioimmunoassay in blood samples drawn before and during running on a level treadmill at 4-8 miles per ...http://circres.ahajournals.org/content/38/6/483
Angiotensin II in rat brain comigrates with authentic angiotensin II in high pressure liquid chromatography. | Circulation...
... a sensitive radioimmunoassay was used to measure extracts of male adult rat brain hypothalamus and cortex after purification ...http://circres.ahajournals.org/content/56/2/212
Repeated sympathetic stimuli elicit the decline and disappearance of prostaglandin modulation and an increase of vascular...
... detected by radioimmunoassay), and plasma catecholamines (detected by high-performance liquid chromatography and ...http://circres.ahajournals.org/content/67/3/580
Phenotyping Hypertrophy | Circulation Research
17 whereas polypeptide expression can be measured immunohistologically or by radioimmunoassay methods.8,9,24 It is not clear ...http://circres.ahajournals.org/content/92/11/1171
Evidence for Hemodynamic Autoregulation of Renin Release | Circulation Research
Renin release, determined by both bioassay and radioimmunoassay, averaged 2.7 ± 0.9 (SE) µg/min at control pressure and ...http://circres.ahajournals.org/content/32/2/237