Evolution of a hot genome | PNAS
Mapping Quantitative Trait Loci Affecting Biochemical and Morphological Fruit Properties in Eggplant (Solanum melongena L.) ... Construction of a SNP-Based Genetic Map Using SLAF-Seq and QTL Analysis of Morphological Traits in Eggplant ... QTL analysis reveals new eggplant loci involved in resistance to fungal wilts ... found a gene that could pave the way toward modifying crops so that they produce stable hybrid seed lines with desirable traits ...
Antibiotic treatment enhances the genome-wide mutation rate of target cells | PNAS
Quantitative PCR reactions used PerfeCTa SYBR Green FastMix (Quanta Biosciences) PCR kit on a Stratagene Mx3000P. The 2−∆∆CT ... The large pool of mutations identified in our MA experiments may include a small subset of candidate resistance loci useful for ... 2014) Insects represent a link between food animal farms and the urban environment for antibiotic resistance traits. Appl ... 2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods ...
Phosphorylation and negative regulation of CONSTITUTIVELY PHOTOMORPHOGENIC 1 by PINOID in Arabidopsis | PNAS
Quantitative real-time PCR analysis shows the expression of PID in Col, PID-CFP Col, PID-GFP Col, myc-mPID Col, and myc-PID Col ... 1991) cop1: A regulatory locus involved in light-controlled development and gene expression in Arabidopsis. Genes Dev 5:1172- ... found a gene that could pave the way toward modifying crops so that they produce stable hybrid seed lines with desirable traits ... I-N) Quantitative real-time PCR analysis showing HY5-regulated gene expression in Col, cop1-6, PID-CFP Col, and PID-GFP Col ...
Buffering of deoxyribonucleotide pool homeostasis by threonine metabolism | PNAS
Quantitative genetic dissection of buffering networks in yeast thus provides a way to model genotype-phenotype variation on a ... There was evidence of gene conversion in 14 of 53 tetrads among the four loci analyzed (including G418R, ClonNatR, and the ... found a gene that could pave the way toward modifying crops so that they produce stable hybrid seed lines with desirable traits ... Similarly, genomic sequences were PCR-amplified and subcloned for targeting to the RNR2 locus: the 3′ promoter or RNR2 was PCR- ...
Nonoverlapping roles of PD-1 and FoxP3 in maintaining immune tolerance in a novel autoimmune pancreatitis mouse model | PNAS
A) Map of the FoxP3-GFPcre locus is shown. Filled boxes, exons; gray box, 3′UTR of Foxp3 gene; I, IRES. (B) Principle of Cre- ... A) CD4+ cells from the SPL and LNs of 2- to ∼3-mo-old indicated mice were subjected for quantitative real-time PCR (qRT-PCR) ... found a gene that could pave the way toward modifying crops so that they produce stable hybrid seed lines with desirable traits ... Quantitative RT-PCR.. Total RNA was extracted by RNeasy Mini Kit (QIAGEN) and reverse-transcribed using reagents included in ...
Patterns, causes, and consequences of marine larval dispersal | PNAS
First, all individuals (n = 7,141) were genotyped at 14 microsatellite loci using the Type-It Microsatellite PCR Kit (Qiagen), ... Although their efficacy largely depends on larval exchange between populations, quantitative analyses of dispersal have been ... found a gene that could pave the way toward modifying crops so that they produce stable hybrid seed lines with desirable traits ... 31). Second, following an initial parentage analysis that identified potential parent-offspring matches based on 14 loci (see ...