Difference between revisions of Griffin:Puromycin Selection - OpenWetWare  Difference between revisions of "Griffin:Puromycin Selection" - OpenWetWare
Puromycin dihydrochloride. Puromycin dihydrochloride is a aminonuclease antibiotic that inhibits protein synthesis. Puromycin ... Puromycin Selection (. shRNA protocol. ) for selection of stably transfected cells. ==. +. Puromycin dihydrochloride is a ... Puromycin selection requires a minimum of 48 hours.. *Monitor the cells daily. Puromycin selection requires a minimum of 48 ... The working puromycin concentration for mammalian cell lines ranges from 1-10 µg/ml. Prior to using the puromycin antibiotic, ...
more infohttps://openwetware.org/wiki/?title=Griffin:Puromycin_Selection&diff=397873&oldid=254859
北京大学医学部机构知识库(IR@PKUHSC): The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside  北京大学医学部机构知识库([email protected]): The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside
The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside. Li, Xuejuan; Zhang, Xiaoyan; Li, Xiaoyan; Ding, ... Li, Xuejuan,et al."The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside".INTERNATIONAL JOURNAL OF ... The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, ... Cyto-protection function of survivin was also studied in cultured podocyte injured by puromycin aminonucleoside (PAN), ...
more infohttp://ir.bjmu.edu.cn/handle/400002259/50714
CiNii 論文 - 
 		
 		
 			
 		 	
 		 		
 		 			Dual Effects of Lsinopril on Purmycin Aminonucleoside Nephrosis in Unilaterally...  CiNii 論文 - Dual Effects of Lsinopril on Purmycin Aminonucleoside Nephrosis in Unilaterally...
Focal and segmental glomerulosclerosis following a single intravenous dose of puromycin aminonucleoside DIAMOND JR. ... The therapeutic effects of angiotensin converting enzyme inhibitor, lisinopril, on puromycin aminonucleoside (PAN)-induced ...
more infohttps://ci.nii.ac.jp/naid/10008677864
Puromycin | Thermo Fisher Scientific - SA  Puromycin | Thermo Fisher Scientific - SA
Puromycin is an aminonucleoside antibiotic produced by the bacterium Streptomyces alboniger. It inhibits protein synthesis by ... Resistance to puromycin is conferred by the puromycin N-acetyl-transferase gene (pac) from Streptomyces. Puromycin has a fast ... Table 1 - Suggested Puromycin Concentration Cell Line Concentration [µg/ml] Reference HT1080 (human fibrosarcoma). 0.2-0.4 1 2 ... Puromycin is an aminonucleoside antibiotic produced by the bacterium Streptomyces alboniger. It inhibits protein synthesis by ...
more infohttps://www.thermofisher.com/sa/en/home/life-science/cell-culture/transfection/selection/puromycin.html
G418, Puromycin, Hygromycin B  G418, Puromycin, Hygromycin B
Antibiotic selection drugs include puromycin, hygromycin, G418 geneticin, neomycin and doxycycline. ... G418, Puromycin, Hygromycin B. Antibiotics for the selection and maintenance of eukaryotic cells that are stably transfected ... G418, Puromycin & Hygromycin Dose Concentrations for Selection & Maintenance. Recommended Concentration (µg/ml) ... Puromycin. *Molecular Weight: 544.4 *Chemical Formula: C22H29N2O5·2HCl *Form: White Powder ...
more infohttp://www.clontech.com/US/Products/Transfection_and_Cell_Culture/Antibiotic_Selection/G418_Puromycin_and_Hygromycin?sitex=10020:22372:US&PROD=WVoWZcG5zrVOujH-1t635537:S&PROD_pses=ZG4AEE8FD5DD52F1C510784CD45BDC47AB32CC84D403BDE0E9DBA7DD91546655AFA1290B969D5AFB3F0295F4B5ADB4EC6B25D89A6C4DCD9788
G418, Puromycin, Hygromycin B  G418, Puromycin, Hygromycin B
Antibiotic selection drugs include puromycin, hygromycin, G418 geneticin, neomycin and doxycycline. ... G418, Puromycin, Hygromycin B. Antibiotics for the selection and maintenance of eukaryotic cells that are stably transfected ... G418, Puromycin & Hygromycin Dose Concentrations for Selection & Maintenance. Recommended Concentration (µg/ml) ... Puromycin. *Molecular Weight: 544.4 *Chemical Formula: C22H29N2O5·2HCl *Form: White Powder ...
more infohttp://www.clontech.com/US/Products/Transfection_and_Cell_Culture/Antibiotic_Selection/G418_Puromycin_and_Hygromycin?sitex=10020:22372:US&PEBCL1=0QAAxSEEZ5LPQWuDhLfFs1dgZq&PEBCL1_pses=ZG316819950075E5D14F18A3858843F84D17F1405802B46BCC0C957771F80FEE068E7AC17F0878459D39CEA4BC783F4B4F3112F820240FD7EC
puromycin in culture medium - Cell Biology  puromycin in culture medium - Cell Biology
puromycin is qualified by my boss as useful for all newbies... That means selection is quite easy. G418 takes a lot of time to ... puromycin in culture medium - (Jan/11/2006 ). Hi everyone. Should I continue add selection pressure in the media if I get the ... for puromycin, i do generally 10j of selection, and stop the antibio.. Then every month/2month, i do a 3day selection. ... for puromycin, i do generally 10j of selection, and stop the antibio.. Then every month/2month, i do a 3day selection. ...
more infohttp://www.protocol-online.org/biology-forums/posts/12648.html
RediFect Red-FLuc-Puromycin | PerkinElmer  RediFect Red-FLuc-Puromycin | PerkinElmer
RediFect Red-FLuc-Puromycin Lentiviral Particles carrying red-shifted Luciola Italica luciferase transgen fused to puromycin ... The luciferase transgene is fused to the puromycin resistance gene via T2A "self-cleaving" linker peptide for efficient co- ... RediFect™ Red-FLuc-Puromycin Lentiviral Particles are self-inactivating, recombination incompetent lentiviral particles ...
more infohttps://www.perkinelmer.com/product/redifect-red-fluc-puromycin-cls960002
LOC440434 (aminopeptidase puromycin sensitive pseudogene)  LOC440434 (aminopeptidase puromycin sensitive pseudogene)
... aminopeptidase puromycin sensitive pseudogene), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol. ...
more infohttp://atlasgeneticsoncology.org/Genes/GC_LOC440434.html
Griffin:Puromycin Selection - OpenWetWare  Griffin:Puromycin Selection - OpenWetWare
Puromycin dihydrochloride. Puromycin dihydrochloride is a aminonuclease antibiotic that inhibits protein synthesis. Puromycin ... Puromycin resistance in mammalian cells. Puromycin Selection post transfection (shRNA)/transduction (lenti). *48 hours post- ... The working puromycin concentration for mammalian cell lines ranges from 1-10 µg/ml. Prior to using the puromycin antibiotic, ... Streptomyces alboniger Puromycin N-acetyltransferase P13249. *Streptomyces alboniger Puromycin N-acetyltransferase. * ...
more infohttps://openwetware.org/wiki/Griffin:Puromycin_Selection
Constructing puromycin kill curve - Tissue and Cell Culture - BioForum  Constructing puromycin kill curve - Tissue and Cell Culture - BioForum
I am planning to construct a puromycin kill curve for my hepatocellular carcinoma cell lines, however, looking at the protocol ... Constructing puromycin kill curve - posted in Tissue and Cell Culture: Dear all, ... Besides, has anyone tried using puromycin on HCC cell lines e.g. SNU-398, SNU-182, HuH-7 etc. before? May I know roughly the ... Besides, has anyone tried using puromycin on HCC cell lines e.g. SNU-398, SNU-182, HuH-7 etc. before? May I know roughly the ...
more infohttp://www.protocol-online.org/forums/topic/11638-constructing-puromycin-kill-curve/
puromycin - oi  puromycin - oi
SP062EFFECTS OF JNK INHIBITOR ON PUROMYCIN AMINONUCLEOSIDE-INDUCED NEPHROPATHY IN RATS Specific bonding of puromycin to full- ... Total synthesis of a xylo-Puromycin analog Ultrastructural study on nephrin expression in experimental puromycin ... Glomerular function and morphology in puromycin aminonucleoside nephropathy in rats Purification and Characterization of a ... Puromycin-sensitive aminopeptidase protects against aggregation-prone proteins via autophagy ...
more infohttp://oxfordindex.oup.com/view/10.1093/oi/authority.20110803100355398
Effect of Puromycin Aminonucleoside on Podocyte P-Cadherin  Effect of Puromycin Aminonucleoside on Podocyte P-Cadherin
Puromycin aminonucleoside (PAN)-induced nephropathy Glomerular epithelial cells (GEpC) Podocyte MeSH Terms expand_less. expand_ ... would be altered by puromycin aminonucleoside (PAN) in a cultured podocyte in vitro. METHODS: Rat glomerular epithelial cells ( ...
more infohttps://koreamed.org/article/0026JKSPN/2013.17.2.79
Puromycin-sensitive Aminopeptidase Protects Against Aggregation-prone Proteins via Autophagy  Puromycin-sensitive Aminopeptidase Protects Against Aggregation-prone Proteins via Autophagy
... Author: Menzies, Fiona M.; Hourez ... Puromycin-sensitive aminopeptidase (PSA) is the only cytosolic enzyme able to digest polyQ sequences. We tested whether PSA can ... Puromycin-sensitive Aminopeptidase Protects Against Aggregation-prone Proteins via Autophagy. DSpace/Manakin Repository. * DASH ... Puromycin-sensitive aminopeptidase protects against aggregation-prone proteins via autophagy. Human Molecular Genetics 19(23): ...
more infohttps://dash.harvard.edu/handle/1/8191187
Puromycin - Wikipedia  Puromycin - Wikipedia
Puromycin-resistant transformants are selected in LB agar medium supplemented with 125 µg/ml of puromycin. But use of puromycin ... Puromycin is soluble in water (50 mg/ml) as colorless solution at 10 mg/ml. Puromycin is stable for one year as solution when ... Also of note, puromycin is critical in mRNA display. In this reaction, a puromycin molecule is chemically attached to the end ... Resistance to puromycin is conferred by the pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a ...
more infohttps://en.wikipedia.org/wiki/Puromycin
pMOD2-Puro : pac puromycin resistance modified gene without CpG | InvivoGen  pMOD2-Puro : pac puromycin resistance modified gene without CpG | InvivoGen
Resistance to the antibiotic puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase isolated from a ... Synthetic Puromycin resistance gene in cloning plasmid. Resistance to the antibiotic puromycin is conferred by the Pac gene ... Synthetic Puromycin resistance gene [Puro-lowCpG]: The Pac gene encoding a puromycin N-acetyl-transferase has been isolated ... Its expression confers puromycin resistance to pac-transfected cells. The size of the Pac gene is small but its CpG content is ...
more infohttps://www.invivogen.com/pmod2-puro
Puromycin Trending Applications: shRNA Gene Silencing Protocol | AG Scientific Blog  Puromycin Trending Applications: shRNA Gene Silencing Protocol | AG Scientific Blog
Puromycins longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the ... NOTE: The working puromycin concentration for mammalian cell lines ranges from 1-10 μg/ml. Prior to using the puromycin ... Puromycins longstanding application as a gene selection antibiotic has a powerful new role in genome editing utilizing the ... Use the lowest concentration that kills 100% of non-transfected cells in 3-5 days from the start of puromycin selection. ...
more infohttps://agscientific.com/blog/2016/06/puromycin-trending-applications-shrna-gene-silencing-protocol/
RediFect Red-FLuc-Puromycin | PerkinElmer  RediFect Red-FLuc-Puromycin | PerkinElmer
RediFect Red-FLuc-Puromycin Lentiviral Particles carrying red-shifted Luciola Italica luciferase transgen fused to puromycin ... The luciferase transgene is fused to the puromycin resistance gene via T2A "self-cleaving" linker peptide for efficient co- ... RediFect™ Red-FLuc-Puromycin Lentiviral Particles are self-inactivating, recombination incompetent lentiviral particles ...
more infohttps://www.perkinelmer.co.uk/product/redifect-red-fluc-puromycin-cls960002
Yi Qi Qing Re Gao Attenuates Podocyte Injury and Inhibits Vascular Endothelial Growth Factor Overexpression in Puromycin...  Yi Qi Qing Re Gao Attenuates Podocyte Injury and Inhibits Vascular Endothelial Growth Factor Overexpression in Puromycin...
H. G. Kang, K. H. Paik, H. Y. Cho et al., "Transcriptome analysis of the response of cultured murine podocytes to puromycin ... A. Suzuki, T. Ito, E. Imai et al., "Retinoids regulate the repairing process of the podocytes in puromycin aminonucleoside- ... D. Wen, L. You, Q. Zhang et al., "Upregulation of nestin protects podocytes from apoptosis induced by puromycin aminonucleoside ... M. Hosoyamada, K. Yan, Y. Nishibori et al., "Nephrin and podocin expression around the onset of puromycin aminonucleoside ...
more infohttps://www.hindawi.com/journals/ecam/2014/375986/ref/