Loading...
*  Reminder effects - reconsolidation or retrieval deficit? Pharmacological dissection with protein synthesis inhibitors following...
It is generally accepted that memory formation involves an irreversible passage via labile phases to the stable form of 'long-term memory' impervious to amnestic agents such as protein synthesis inhibitors. However, recent experiments demonstrate that reactivation of memory by way of a reminder renders it labile to such inhibitors, suggesting that such retrieval is followed by a so-called reconsolidation process similar or identical in its cellular and molecular correlates to that occurring during the initial consolidation. We compared the effects of the protein synthesis inhibitor anisomycin and the glycoprotein synthesis inhibitor 2-deoxygalactose on the temporal dynamics and pharmacological sensitivity of initial consolidation and memory expression following a reminder in a one-trial passive-avoidance task in day-old chicks. This comparison revealed three differences ...
http://oro.open.ac.uk/6716/
*  Most recent papers with the keyword cryptical biosynthesis | Read by QxMD
The protein synthesis inhibitor anisomycin features a unique benzylpyrrolidine system and exhibits diverse biological and pharmacologic activities. Its biosynthetic origin has remained obscure for more than 60 y, however. Here we report the identification of the biosynthetic gene cluster (BGC) of anisomycin in Streptomyces hygrospinosus var. beijingensis by a bioactivity-guided high-throughput screening method. Using a combination of bioinformatic analysis, reverse genetics, chemical analysis, and in vitro biochemical assays, we have identified a core four-gene ensemble responsible for the synthesis of the pyrrolidine system in anisomycin: aniQ, encoding a aminotransferase that catalyzes an initial deamination and a later reamination steps; aniP, encoding a transketolase implicated to bring together an glycolysis intermediate with 4-hydroxyphenylpyruvic acid to form the anisomycin molecular backbone; aniO, encoding a glycosyltransferase that catalyzes a ...
https://www.readbyqxmd.com/keyword/95129
*  The effects of anisomycin (a protein synthesis inhibitor) on spatial learning and memory in CA1 region of rats hippocampus
Studies were performed to investigate whether electrically-induced long-term depression (LTD) within rat hippocampal slices in vitro shares any common cellular features with LTD in the intact animal, with particular emphasis being placed on mechanisms required for its late maintenance. Our initial studies have led to the development of stimulation protocols which are able to reliably produce different forms of LTD. Depending on the induction protocol applied, we are able to demonstrate a transient protein synthesis-independent early-LTD with a duration of up to 3-4 h, together with a de novo protein synthesis-dependent late-LTD lasting for at least 8 h. Furthermore, we are able to show input-specific LTD within the CA1 region, with expression shown only by those synapses specifically stimulated by a low-frequency protocol. These studies are important pre-requisites to investigate mechanisms of 'synaptic tagging' and 'late-associativity' ...
http://anisomycin.noneto.com/a3.htm
*  Hypoxic neuronal necrosis: Protein synthesis-independent activation of a cell death program | Proceedings of the National...
In DG cultures exposed to 10 mM NaCN for 45 min, ≈90% of neurons (identified by immunoreactivity against NeuN, a neuronal marker) died 24 h after hypoxia, because they stained with propidium iodide. Survival of GFAP-immunoreactive glia was not affected (data not shown). Combining the same cyanide treatment with glucose deprivation ("ischemia") had very similar effects on the neurons. By EM, many hypoxic neurons showed early swelling of mitochondria and endoplasmic reticulum, in the presence of an intact nucleus (Fig. 1B), and evolved later toward necrosis with severe cytoplasmic swelling and membrane rupture (Fig. 1C). This finding was in sharp contrast to staurosporine-treated apoptotic neurons, which displayed nuclear breakdown into large, round masses of chromatin (Fig. 1D). Hypoxic necrosis evolved rapidly: we observed a progressive loss of the mitochondrial membrane potential Δψ within a few minutes of cyanide application (Fig. 2 A-D). This Δψ decay was always synchronous with release ...
http://www.pnas.org/content/100/5/2825
*  Protein synthesis inhibition and GADD34 control IFN‐β heterogeneous expression in response to dsRNA | The EMBO Journal
In innate immune responses, induction of type‐I interferons (IFNs) prevents virus spreading while viral replication is delayed by protein synthesis inhibition. We asked how cells perform these apparently contradictory activities. Using single fibroblast monitoring by flow cytometry and mathematical modeling, we demonstrate that type‐I IFN production is linked to cell's ability to enter dsRNA‐activated PKR‐dependent translational arrest and then overcome this inhibition by decreasing eIF2α phosphorylation through phosphatase 1c cofactor GADD34 (Ppp1r15a) expression. GADD34 expression, shown here to be dependent on the IRF3 transcription factor, is responsible for a biochemical cycle permitting pulse of IFN synthesis to occur in cells undergoing protein synthesis inhibition. Translation arrest is further demonstrated to be key for anti‐viral response by acting synergistically with MAVS activation to amplify TBK1 signaling and ...
http://emboj.embopress.org/content/early/2017/01/18/embj.201695000
*  Protein Synthesis Worksheet. Worksheets. Doriandnimo Thousands of Printable Activities
Protein Synthesis Worksheet. Worksheets. Protein synthesis worksheet. Protein synthesis worksheet exercises key 3 png school png. Dna protein synthesis worksheet. Protein synthesis worksheet page 2 biology and chemistry stuff 1. Protein synthesis worksheet hot resources 12 17 worksheet. Protein synthesis worksheet 9th 12th grade lesson planet. Protein synthesis worksheet answer key abitlikethis codons your body knows where to. Of protein synthesis worksheet answer key sharebrowse collection sharebrowse. Protein synthesis. Protein synthesis worksheet codons your body knows where to 3 pages 1. Dna and protein ...
http://doriandnimo.com/protein-synthesis-worksheet/
*  Studies Reveal How Cells Distinguish Between Disease-Causing and Innocuous Invaders
Troemel's team of researchers-who included Tiffany Dunbar, Zhi Yan, Keir Balla and Margery Smelkinson-found in their experiments that a particular genetic system-the "ZIP-2 surveillance pathway"-was used by the roundworm in detecting an infection by the disease-causing bacterium Pseudomonas aeruginosa. The biologists also found that a specific toxin in the bacterium-"Exotoxin A"-blocks protein synthesis in the worm's intestine.. "Surprisingly, this block leads to increased protein levels of the ZIP-2 transcription factor to ultimately induce expression of defense genes," the scientists conclude in their paper. "Thus, a common form of pathogen attack acts to switch on host defense, allowing discrimination of pathogens from innocuous microbes.". "In addition to P. aeruginosa Exotoxin A," said Troemel, "there are several other bacterial toxins known to block protein synthesis, such as Diphtheria toxin, Ricin toxin and Shiga ...
http://ucsdnews.ucsd.edu/pressrelease/studies_reveal_how_cells_distinguish_between_disease_causing_and_innocuous
*  IJMS | Free Full-Text | Protein Synthesis Inhibition Activity by Strawberry Tissue Protein Extracts during Plant Life Cycle...
Ribosome-inactivating proteins (RIPs), enzymes that are widely distributed in the plant kingdom, inhibit protein synthesis by depurinating rRNA and many other polynucleotidic substrates. Although RIPs show antiviral, antifungal, and insecticidal activities, their biological and physiological roles are not completely understood. Additionally, it has been described that RIP expression is augmented under stressful conditions. In this study, we evaluated protein synthesis inhibition activity in partially purified basic proteins (hereafter referred to as RIP activity) from tissue extracts of Fragaria × ananassa (strawberry) cultivars with low (Dora) and high (Record) tolerance to root pathogens and fructification stress. Association between the presence of RIP activity and the crop management (organic or integrated soil), growth stage (quiescence, flowering, and fructification), and exogenous stress (drought) ...
http://www.mdpi.com/1422-0067/14/8/15532/notes
*  "Prevalent deficiency in tumor cells of cycloheximide-induced cycle arr" by E E. Medrano and A B. Pardee
Medrano, E E. and Pardee, A B., "Prevalent deficiency in tumor cells of cycloheximide-induced cycle arrest." (1980). Subject Strain Bibliography 1980. 3388 ...
https://mouseion.jax.org/ssbb1980/3388/
*  AID 327182 - Inhibition of JNK1 in rat H9c2 cells assessed as inhibition of anisomycin-induced cJun phosphorylation after 30...
BioAssay record AID 327182 submitted by ChEMBL: Inhibition of JNK1 in rat H9c2 cells assessed as inhibition of anisomycin-induced cJun phosphorylation after 30 mins.
https://pubchem.ncbi.nlm.nih.gov/bioassay/327182
*  Plouhinec JL et al. (2014), Pax3 and Zic1 trigger the early neural crest ge... - Xenbase Paper
Fig. 5. Identification of Pax3 binding sites on Pax3 promoters in vitro (A) Pax3, but not Zic1 alone, can trigger synthesis of the endogenous Pax3 transcript (primers in 3'UTR, not amplifying the inducible form) either in absence of the translation inhibitor cycloheximide (induction is 5-fold compared to uninjected animal caps) or in the presence of cycloheximide (induction is 31-fold, compared to cycloheximide-treated uninjected animal caps). Endogenous pax3 expression level in a stage 11 whole embryo is set at a relative value of 1 unit. After co-injection of Pax3 and Zic1, endogenous pax3 was activated as an immediate-early target as well (77-fold compared to uninjected animal caps; and, in presence of cycloheximide, 16-fold when compared to cycloheximide-treated uninjected animal caps). (B) Location of the ECR containing Pax3 putative binding site in the genomic sequence upstream of the Pax3 TSS. (C) Pax3 binds specifically to the motif identified in the pax3 promoter ECR: an ...
http://www.xenbase.org/literature/article.do?method=display&articleId=48413
*  Plus it
Treatment with the protein synthesis inhibitor cycloheximide (CHX) or silencing of the core component of the NMD machinery, Upf-1 (also known as Rent1), are widely used to determine if transcripts are subject to NMD (Montfort et al., 2006). CHX is an indirect NMD inhibitor since NMD activation is posttranscriptional, but translation-dependent, and ribosomal association is required during the pioneer round of translation. Upf-1 silencing is believed to produce direct suppression of NMD. The RNA helicase, Upf-1, is a central effector of NMD that links the translation-termination event to the assembly of a surveillance complex. Upf2 and Upf3 are believed to recruit and activate Upf1 on NMD substrates (Lykke-Andersen et al., 2000). Although the discrimination of PTCs from normal termination codons and the molecular links that trigger NMD are still unclear, it is well established that the core components of the NMD machinery are the conserved proteins, Upf-1, ...
http://www.jneurosci.org/content/29/9/2833
*  Plus it
We have investigated the effect of chemotherapeutic and DNA damaging agents on binding of the tumor suppressor phosphoprotein p53 to its consensus DNA sequence. Activation of p53-DNA binding was seen for treatment with radiation, hydrogen peroxide, actinomycin D, Adriamycin, etoposide, camptothecin, 5-fluorouracil, mitomycin C, and cisplatin. These results showed that DNA strand breaks were sufficient to lead to increased levels of p53. The protein synthesis inhibitor cycloheximide blocks the increase in p53 following DNA damage. The increase in p53 activation in camptothecin treated cells may result, at least in part, from an increased half-life of the protein and consequent increases in intracellular protein concentration.. ...
http://cancerres.aacrjournals.org/content/53/10/2212
*  Cycloheximide - Molecular Biology
Hi,. Would really appreciate if somone could help me understand the role of treating cells with cycloheximide? Is this a protein synthesis inhibitor ? If for example, a particular treatment (e.g. treatment X) decreases beta-catenin protein levels, investigators treat cells with treatment X alone or in combination with Cycloheximide to determine if beta-catenin inhibition is a result of degradation? Why do they mention degradation if Cycloheximide is a protein synthesis inhibitor? The journals mention that beta catenin is down regulated after treatmemnt with X and also in combination Cycloheximide, therefore suggesting inhibition of beta catenin is a result of increased degradation? I thought MG132 is used to determine degradation by proteasome?. ...
http://www.protocol-online.org/biology-forums-2/posts/31358.html
*  ASMscience | Mechanism Explaining Low-Level Strep Resistance Raises Bigger Questions
Although high-level resistance to streptomycin arises from mutations in a ribosomal protein, the source of low-level resistance to this antibiotic remained obscure for several decades. Now that mystery is solved, although it raises several others, according to Kenji Nishimura, Susumu Okamoto, and others who work with Kozo Ochi at the National Food Research Institute, Tsukuba, Japan. They report that low-level streptomycin resistance arises because of a mutation to a gene encoding a specific methyl transferase that, in turn, leaves a particular nucleotide unmethylated at the site along the ribosome called the 530 loop. In this unmethylated state, the ribosome remains capable of making proteins even in the presence of streptomycin, which ordinarily blocks protein synthesis.
http://www.asmscience.org/content/journal/microbe/10.1128/microbe.2.278.1
*  Plus it
Previous studies have led to the hypothesis that some proteins of the postsynaptic membrane are locally synthesized at postsynaptic sites. To evaluate this hypothesis, synaptosome fractions that included fragments of dendrites were allowed to incorporate labeled amino acid into protein. The labeled synaptosomes were then subfractionated to the level of the synaptic plasma membrane (SPM) and then the synaptic junctional complex (SJC). The specific activity (cpm/microgram protein) of the synaptosome fraction and its subfractions was assessed by scintillation counting and protein assay, and labeled polypeptides were characterized by SDS-PAGE and fluorography. The contribution of mitochondrial and eucaryotic protein synthesis to the overall incorporation was evaluated using cycloheximide (CYC), a eucaryotic protein synthesis inhibitor, and chloramphenicol (CAP), a mitochondrial ...
http://www.jneurosci.org/content/11/9/2881
*  Anisomycin | Abcam
Buy Anisomycin (CAS 22862-76-6), a protein synthesis inhibitor. Join researchers using high quality Anisomycin from Abcam and achieve your mission, faster.
http://www.abcam.com/anisomycin-ab120495.html
*  Anisomycin|Flagecidin;Wuningmeisu C|CAS 22862-76-6 Buy Anisomycin from supplier medchemexpress.com
Anisomycin is a pyrrolidine antibiotic, acts as an anti-fungal antibiotic which inhibits Protein Synthesis, also is a potent activator of SAPKs/JNKs. ...Quality confirmed by NMR,HPLC & MS.
http://www.medchemexpress.com/Anisomycin.html
*  science protein synthesis Flashcards - Cram.com
Study Flashcards On science protein synthesis at Cram.com. Quickly memorize the terms, phrases and much more. Cram.com makes it easy to get the grade you want!
http://www.cram.com/flashcards/science-protein-synthesis-317139
*  Biology-Online • View topic - Qs: protein synthesis, plant, animal ,and eukaryotic cells
1. In the process of protein synthesis, an enzyme will trigger a sequence of triplets of bases that encodes a protein, which is called a ______, to unzip from the segment of the DNA to produce a mRNA. This process is also called _______.. Then the mRNA will be decodes by the ______, and this step is called ...
http://www.biology-online.org/biology-forum/about24895.html?p=141965&hilit=Ribosome
*  What Does the Term "protein Synthesis" Mean? | Reference.com
The term 'protein synthesis' refers to the making of a protein. This process begins in the nucleus and then continues in the ribosomes. Protein synthesis is comprised of two parts: DNA transcription...
https://www.reference.com/science/term-protein-synthesis-mean-fa7ea0055954d7f6
*  Regulation of Protein Synthesis by Calcium - Charles Brostrom
The fundamental, continuing goal of the proposed research is to investigate the potential importance of Ca2+ as a physiologic regulator of protein synthesis in...
http://grantome.com/grant/NIH/R01-DK035393-06
*  Why is protein synthesis important? | Reference.com
Protein synthesis is important because the proteins created during this process control the activities of the cells. Without these proteins, many of the processes in the body would fail or not work...
https://www.reference.com/science/protein-synthesis-important-7773af281bbbc68f
*  cycloheximide | VWR
Learn more about cycloheximide. We enable science by offering product choice, services, process excellence and our people make it happen.
https://us.vwr.com/store/product/2298903/cycloheximide