Protein Multimerization / drug effects * Reproducibility of Results * Small Molecule Libraries* * Systems Biology / methods* ...

As is the case for other G protein-coupled … ... Protein Binding * Protein Multimerization* * Receptors, ... As is the case for other G protein-coupled receptors, chemokine receptors are not isolated entities that are activated ...

In this study, we describe a protein nanoparticle vaccine against SARS-CoV-2. The vaccine is based on the display of ... A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces ... A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces ... In this study, we describe a protein nanoparticle vaccine against SARS-CoV-2. The vaccine is based on the display of ...

"Oxidative stress/damage induces multimerization and interaction of Fanconi anemia proteins". J. Biol. Chem. 279 (29): 30053-9. ... Amongst known FANC proteins, most evidence points for a direct interaction primarily between FANCA protein and BRCA1. Evidence ... This mechanic is also supported by the protein-protein interactions between BRG1 and both BRCA1 and FANCA, that serve to ... Gordon SM, Buchwald M (2003). "Fanconi anemia protein complex: mapping protein interactions in the yeast 2- and 3-hybrid ...

However, the procedure for protein expression is inefficient and needs to be improved. Herein, we propose a simple construct ... Recombinant proteins produced by the baculovirus expression vector system (BVES) have been widely applied in the agricultural ... that incorporates a selectable marker (enhanced green fluorescent protein, EGFP) and a picorna viral-derived ... For multimerization analysis, the purified ADN was subjected to SDS-PAGE with or without 95 °C heat denaturation for 10 min, ...

... multimerization, protein priming, and nucleotide preference." Curr Genet, 64(6), 1287-1301. doi:10.1007/s00294-018-0844-. ... Gribble, K. E., Snell, T. W., & Mark Welch, D. B. (2011). "Gene and protein structure of the mate recognition protein gene ... Gribble, K. E., & Mark Welch, D. B. (2012). "The mate recognition protein gene mediates reproductive isolation and speciation ... "Ancient association between cation leak channels and Mid1 proteins is conserved in fungi and animals." Front Mol Neurosci, 7, ...

... and discuss how this molecule infl uences the retention and clustering of diverse synaptic proteins to regulate synaptic ... and discuss how this molecule influences the retention and clustering of diverse synaptic proteins to regulate synaptic ... and protein-mediated multimerization of PSD-95: implications for receptor clustering and assembly of synaptic protein networks ... Numerous studies hint at an important role for PSD-95 multimerization in regulation of protein trafficking and ion channel ...

Filters: Keyword is Protein Multimerization and Author is Guo, Youzhong [Clear All Filters] ... Protein structure. Structure and activity of tryptophan-rich TSPO proteins. Science. 2015 ;347(6221):551-5. ...

LATE BLOCK DURING VIRAL REPLICATION AND ABNORMAL MULTIMERIZATION INVOLVING SPECIFIC PROTEIN DOMAINS. J Biol Chem. 2014 Jul 25; ... Viral DNA tethering domains complement replication-defective mutations in the p12 protein of MuLV Gag. Proc Natl Acad Sci U S A ... Altering murine leukemia virus integration through disruption of the integrase and BET protein family interaction. Nucleic ... BET proteins promote efficient murine leukemia virus integration at transcription start sites. Proc Natl Acad Sci U S A. 2013 ...

Levels of proteins, including Cyt C, Bcl-2/BAX, and Nrf2/HO-1-associated proteins, were determined by western blotting and ... ARC harbors a retrovirus-like Gag domain that facilitates ARC multimerization and capsid formation. Trans-synaptic transfer of ... Polycomb group proteins assemble into multi-protein complexes, known as Polycomb repressive complexes 1 and 2 (PRC1 and PRC2), ... This study identified the EF-hand domain containing protein EFhD2/Swip-1 as a conserved lamellipodial protein strongly ...

Post-translational covalent attachment of cholesterol and palmitate to Hh proteins are critical for multimerization and long ... The Hedgehog (Hh) family of secreted proteins act as morphogens to control embryonic patterning and development in a variety of ... Altogether, these results shed new light on the mechanisms of action of Hh proteins. Furthermore, they provide the first ... domain and experimentally disrupted the ability of HHAT to palmitoylate Hh proteins such as DHH and SHH. Consistent with the ...

Use of reducing conditions with SDS-PAGE can decrease the extent of multimerization. ... Protein post-translational cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active/mature ... with larger proteins migrating more slowly than smaller proteins. Loading protein markers of established size (e.g., Trident ... Protein multimers: Target proteins may form multimers (dimers, trimers, and so on) and display one or more bands of increased ...

We discuss the implications of multimerization for the function of POZ domain proteins. ... Click on the protein counts, or double click on taxonomic names to display all proteins containing BTB domain in the selected ... Known to be a protein-protein interaction motif found at the N-termini of several C2H2-type transcription factors as well as ... The POZ domain acts as a specific protein-protein interaction domain: The POZ domains of ZID and Ttk can interact with ...

Mutations in the three SHANK genes encoding for postsynaptic scaffolding proteins have been shown to represent risk factors for ... have suggested that proteins of the postsynaptic density of excitatory synapses have a role in its etiology. ... alpha motif domain proposes a possible effect on the dendritic localization and the self-multimerization of the mutant protein ... By numerous specific protein-protein interactions, SHANKs are either directly or indirectly linked to other structural proteins ...

Combining in silico and experimental approaches to identify and understand the residue changes in the H-NS protein that allowed ... Modeling and in vitro analysis of autoinhibition and multimerization of histone-like nucleoid-structuring (H-NS) orthologs.. (A ... A) The 1H-15N HSQC spectrum of 200 µM 13C,15N-labeled S. typhimurium H-NS84-137 protein in Apo (orange) and H-NS1-57 saturated ... The two protein chains forming the dimer are color-coded. The site2 sequence alignment indicates in red the residue sites that ...

Lipid- and protein-mediated multimerization of PSD-95: implications for receptor clustering and assembly of synaptic protein ... Four litres of 2×YT media were used for protein expression at 22°C. The protein was purified using two affinity columns as ... Fatty acylation of proteins: new insights into membrane targeting of myristoylated and palmitoylated proteins. Biochim. Biophys ... such as GPI-anchored proteins, H-Ras, Src-family tyrosine kinases, eNOS, and G-protein alpha-, beta-, and gamma-subunits. ...

As evaluated by co-immunoprecipitation experiments, the dsDNA recombination function relates to the Redα-Redβ protein-protein ... Single strand annealing proteins (SSAPs) like Redβ initiate homologous recombination by annealing complementary DNA strands. We ... multimerization to form rings and a similar protein architecture based on an N-terminal DNA annealing domain with C-terminal ... However it may also be due to the complexity of the protein-protein interaction. In particular we discovered that the protein- ...

We demonstrate that DVL dishevelled and axin (DIX) domain, but not DIX domain-mediated multimerization, is essential for DVLs ... and centrosomal Nek2-associated protein 1 (C-NAP1), two proteins of the centrosomal linker. Displacement of DVL from the ... Dishevelled (DVL) is a key scaffolding protein and a branching point in Wnt signaling pathways. Here, we present conclusive ... Dishevelled is a NEK2 kinase substrate controlling dynamics of centrosomal linker proteins. ...

We found that protein NCs were readily detected in all samples tested, including multiprotein complexes and membrane proteins. ... RC1 conceals non-conserved immunodominant regions by the addition of glycans and/or multimerization on virus-like particles. ... of the host ubiquitin-proteasome-mediated protein degradation pathway. The molecular details of how Vpr usurps the protein ... The entry of the virus into cells depends on the receptor-binding domain (RBD) of the spike (S) protein of SARS-CoV-2. Although ...

Immunoprecipitation for Native Proteins. This protocol is intended for immunoprecipitation of native proteins for analysis by ... Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently ... A cell lysate pre-clearing step is highly recommended to reduce non-specific protein binding to the Protein A Magnetic beads. ... Resuspend ChIP-Grade Protein G Magnetic Beads #9006 by gently vortexing. Immediately add 30 µl of Protein G Magnetic Beads to ...

The assembly steps include protein trafficking to the plasma membrane, homo-multimerization that induced protein enrichment, ... EBNA-1 binding protein 2 (EBP2), a nucleolar protein, has been proposed to act as a docking protein for EBNA-1 on mitotic ... Additionally, target proteins can be expressed as enhanced green fluorescent protein (EGFP)-fusion proteins. To assess the ... through interaction with endocytic proteins. Here, we show that protein interacting with C terminal kinase 1 (PICK1), a protein ...

This leads to the abnormal multimerization and extracellular deposition of mutant Notch3-ECD at the plasma membrane of smooth ... Kast, Jessica (2014): Involvement of latent TGF-beta binding protein 1 in CADASIL-relevant Notch3 aggregation. Dissertation, ... Involvement of latent TGF-beta binding protein 1 in CADASIL-relevant Notch3 aggregation ... Involvement of latent TGF-beta binding protein 1 in CADASIL-relevant Notch3 aggregation ...

... increased affinity for von Willebrand protein in the resting stage leads to the deletion of plasma von Willebrand protein. This ... Type IIM von Willebrand disease is due to a defect in binding to platelet glycoprotein Ib but no defect in multimerization. In ... Evidence indicates that TTP results from the abnormal presence of unusually large multimers of von Willebrand protein. [17] ... Plasmapheresis and extracorporeal protein A adsorption have been tried in desperate situations. The autoantibodies responsible ...

By studying Ste12 multimerization, I found that this process might involve dephosphorylation on Ste12 to regulate the ... I discovered that Cdc55, a regulatory subunit of protein phosphatase IIA, can affect pheromone response. In the cdc55 null ... By studying Ste12 multimerization, I found that this process might involve dephosphorylation on Ste12 to regulate the ... I discovered that Cdc55, a regulatory subunit of protein phosphatase IIA, can affect pheromone response. In the cdc55 null ...

... increased affinity for von Willebrand protein in the resting stage leads to the deletion of plasma von Willebrand protein. This ... Type IIM von Willebrand disease is due to a defect in binding to platelet glycoprotein Ib but no defect in multimerization. In ... Evidence indicates that TTP results from the abnormal presence of unusually large multimers of von Willebrand protein. [17] ... Plasmapheresis and extracorporeal protein A adsorption have been tried in desperate situations. The autoantibodies responsible ...

Identification of pathways deregulating neuronal ELAV/Hu RNA binding proteins and alternative splicing in neurodegeneration at ... it is thought that multimerization of ELAV/Hu proteins is key to generate target specificity and that this step is highly ... ELAV/Hu proteins comprise a family of highly conserved neuronal RNA binding proteins important for the development of the ... Indeed, ELAV/Hu proteins have numerous sites for post-translational modifications. From initial studies on human Hu proteins, ...

Multimerization of the nanobodies led to agglutination of C. jejuni cells, which may affect colonization in the chicken gut. ... The target of the nanobodies was identified as the major outer membrane protein, a porin that contributes to bacterial ... Multimerization of nanobodies can increase the retention. The results show that multimerization of the anti-Campylobacter ... Additional file 2. LC-MS/MS identifies the MOMP as a target for the nanobodies. Proteins recognized by the anti-Campylobacter ...

... nonstructural protein 3A plays important roles in virus replication, virulence, and host range. In other picornaviruses, ... including proteins relevant for virus replication (36). In this line, dimerization/multimerization has been shown to be ... we set up an in situ protein ligation assay previously used to visualize protein-protein interaction in the cell by ... This homodimerization of 3A was also evidenced by an in situ protein ligation assay designed to visualize protein-protein ...

Protein Multimerization. , Protein Structure, Tertiary. , Receptors, Notch. Citation. Persistent link. http://hdl.handle.net/ ...

Proteins with a VWFD domain are listed below:. * Mammalian von Willebrand factor (VWF), a multifunctional protein involved in ... The type D domain (VWFD) is not only required for blood clotting factor VIII binding but also for normal multimerization of VWF ... Click on the protein counts, or double click on taxonomic names to display all proteins containing VWD domain in the selected ... Mammalian bone morphogenetic protein-binding (BMP-binding) endothelial regulator protein.. * Mammalian alpha-tectorin, which is ...