Prokaryotic Initiation Factor-2
     Summary Report | CureHunter  Prokaryotic Initiation Factor-2 Summary Report | CureHunter
Prokaryotic Initiation Factor-2: The largest of the three prokaryotic initiation factors with a molecular size of approximately 80 kD. It functions in the transcription initiation process by promoting the binding of formylmethionine-tRNA to the P-site of the 30S ribosome and by preventing the incorrect binding of elongator tRNA to the translation initiation site.
more infohttp://www.curehunter.com/public/keywordSummaryD039881-Prokaryotic-Initiation-Factor-2.do
Prokaryotic small ribosomal subunit - Wikipedia  Prokaryotic small ribosomal subunit - Wikipedia
The prokaryotic small ribosomal subunit, or 30S, is the smaller subunit of the 70S ribosome found in prokaryotes. It is a complex of the 16S ribosomal RNA (rRNA) and 19 proteins. This complex is implicated in the binding of Transfer RNA onto the messenger RNA (mRNA). The small subunit is responsible for the binding and the reading of the mRNA during translation. The whole subunit, both the rRNA and the 22 proteins, complexes with the larger 50S subunit to form the 70S prokaryotic ribosome in the cell. This 70S ribosome is then used to translate mRNA into proteins. The 30S subunit is an integral part of mRNA translation. It binds three Prokaryotic initiation factors: IF-1, IF-2, and IF-3. A portion of the 30S subunit (the 16S rRNA) guides the initiating (5')-AUG-(3') (start codon) codon of the mRNA into position by a recognizing a complimentary binding site, about 8 base pairs upstream, known as the Shine-Dalgarno (SD) ...
more infohttps://en.wikipedia.org/wiki/Prokaryotic_small_ribosomal_subunit
Translation - The School of Biomedical Sciences Wiki  Translation - The School of Biomedical Sciences Wiki
There are 3 main steps in Prokaryotic translation; Initiation, Elongation and Termination. Initiation: This involves the initiation factors IF1, IF2, IF3; GTP is required for energy. IF1 and IF3 bind to the free 30S subunit, releasing it from the 50S subunit. IF2 forms a complex with GTP and binds to the 30S subunit, which attaches to an mRNA molecule. mRNA has a ribosome binding site (RBS), which is adjacent to the stat codon AUG. The start codon is approximately 7-10 nucleotides away from the RBS. It is important to note that the 30S subunit is complementary to the ribosome binding site, so base pairing can occur with the 16S rRNA. A charged initiator tRNA (fMet-tRNAfmet), then binds to this start codon. IF3 is released, allowing a 50S subunit to bind to the 30S complex to form the 70S initiation complex which has a P (peptidyl) and A (acceptor) site . During this formation, IF1 is released and both IF2 ...
more infohttps://teaching.ncl.ac.uk/bms/wiki/index.php?title=Translation&diff=prev&oldid=5367
Stringent Response: mitochondrial translation - complete mess  Stringent Response: mitochondrial translation - complete mess
Mitochondria do not have IF1 (all mitochondria), and unlike mammals, yeast ones do not have IF3! Also they seem to use mitochondria-specific initiation factor AEP3. Do mammals have AEP3 homologue? Worth checking... To make things more complicated, mitochondria have their own special mRNA-specific factors... and again, there is a catch. Yeast mRNA have long 5' UTRs (untranslated regions), and mammals have short, they basically have leaderless mRNAs (I'd love to see a good reference for that! THIS is a little bit out of date...) - therefore I would expect that these mRNA-specific IFs work differently in yeast in mammals. So yeast and mammalian mitochondrial translation seems to have very, very different translational apparatus. Isn't is weird ...
more infohttp://stringentresponse.blogspot.com/2011/01/mitochondrial-translation-complete-mess.html
PPT - PROTEIN     SYNTHESIS PowerPoint presentation | free to download  - id: 3dec9c-YzFhZ  PPT - PROTEIN SYNTHESIS PowerPoint presentation | free to download - id: 3dec9c-YzFhZ
PROTEIN SYNTHESIS Protein synthesis Aspects of protein synthesis Mechanism of protein synthesis (Prokaryotic) Initiation in eukaryotes Translational control and post ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 3dec9c-YzFhZ
more infohttp://www.powershow.com/view/3dec9c-YzFhZ/PROTEIN_SYNTHESIS_powerpoint_ppt_presentation
ybey TaqMan SNP Genotyping Assays  ybey TaqMan SNP Genotyping Assays
71 ybey TaqMan 5'-nuclease assay chemistry provides a fast and simple way to get single nucleotide polymorphism (SNP) genotyping results.
more infohttps://www.thermofisher.com/order/genome-database/browse/genotyping/keyword/YBEY
Evolution of relative reading frame bias in unidirectional prokaryotic gene overlaps  - WRAP: Warwick Research Archive Portal  Evolution of relative reading frame bias in unidirectional prokaryotic gene overlaps - WRAP: Warwick Research Archive Portal
Pairs of unidirectional (same strand) genes can overlap in one of two phases (relative reading frames). There is a striking bias in the relative abundance of prokaryotic gene overlaps in the two possible phases. A simple model is presented based on unidirectional gene overlaps evolving from nonoverlapping gene pairs, through the adoption of alternative start codons by the downstream genes. Potential alternative start codons within upstream gene sequences were found to occur at greater frequencies in one phase, corresponding to the most prevalent phase of gene overlaps. We therefore suggest that the phase bias of overlapping genes is primarily a consequence of the N-terminal extension of downstream genes through adoption of new start codons.. ...
more infohttp://wrap.warwick.ac.uk/6068/
Prokaryotic Gene Expression  - Simon Baumberg - Oxford University Press  Prokaryotic Gene Expression - Simon Baumberg - Oxford University Press
Although prokaryotes (which include bacteria) lack a nucleus, they have genomic DNA and the study of their genes is an important area of research, with implications for developmental biology, the study of cancer, and the development of antibiotics. This book, written for graduates and researchers but accessible to advanced undergraduates as well, provides a broad review of current research.
more infohttps://global.oup.com/academic/product/prokaryotic-gene-expression-9780199636037?cc=us&lang=en&tab=toc
American Urological Association - Guidelines  American Urological Association - Guidelines
The AUA's Clinical Practice Guidelines provide evidence-based guidance with an explicit clinical scope and purpose. AUA also provides Policy Statements, Best Practice Statements, Position Statements and White Papers to provide urology professionals with the best in peer-reviewed treatment recommendations and research.
more infohttp://www.auanet.org/guidelines?q=&ContentType=Clinical_Guidelines%7C&filters=772%7C783%7C770%7C
Nucleic Acid-Protein Interactions in Turnip Yellow Mosaic Virus | Science  Nucleic Acid-Protein Interactions in Turnip Yellow Mosaic Virus | Science
Mild alkaline treatment induces the formation of a specific RNA complex inside turnip yellow mosaic virus. Dialysis at pH 4.5 destroys this complex inside the capsid, but does not destroy complexes that have been removed from their capsids. These facts were interpreted in terms of a breakage and subsequent re-formation of RNA-protein links. In combination with supporting potentiometric data, they suggest histidinyl-phosphate salt links or carboxylate-amino hydrogen bonds, or both, as the principal means of association of turnip yellow mosaic virus protein and RNA. ...
more infohttp://science.sciencemag.org/content/166/3902/248
Preeti Pancholi, PhD :: Center for Microbial Interface Biology  Preeti Pancholi, PhD :: Center for Microbial Interface Biology
Kapur P, Ahuja SP, Mohan R, Gupta PP, Sobti VK. "Vasectomy induces changes in the serum and tissues of rabbits." Ann Biol. Vol. 1, (January 1985.): 201-208.. Kapur P, Ahuja SP, Mohan R, Gupta PP. "Vasectomy induced changes in lipid metabolism of different organs of rabbits, with or without feeding of arthrogeneic diet." J Nuclear Agri Biol. Vol. 14, (January 1985.): 112-114.. Pancholi P, Vinayak VK, Khuller GK. "Immunogenicity of ribonucleic acid-protein fraction of Mycobacterium tuberculosis encapsulated in liposomes." J Med Microbiol. Vol. 29, (January 1989.): 131-138.. Pancholi P, Vinayak VK, Khuller GK. "Immunogenicity of ribonucleic acid protein fraction of mycobacterium-tuberculosis encapsulated in liposomes." JOURNAL OF MEDICAL MICROBIOLOGY. Vol. 29, no. 2. (June 1989.): 131-138.. Pancholi P, Vinayak VK, Khuller GK. "Immunobiological studies with mycobacterial ribonucleic acid-protein complex: Part I--Immune responses and protective role against experimental tuberculosis." Indian J Exp ...
more infohttps://cmib.osu.edu/people/faculty/preetipancholi/index.cfm
PubMed medline query  PubMed medline query
Unique DNA sequences can be determined directly from mouse genomic DNA. A denaturing gel separates by size mixtures of unlabeled DNA fragments from complete restriction and partial chemical cleavages of the entire genome. These lanes of DNA are transferred and UV-crosslinked to nylon membranes. Hybridization with a short 32P-labeled single-stranded probe produces the image of a DNA sequence "ladder" extending from the 3' or 5' end of one restriction site in the genome. Numerous different sequences can be obtained from a single membrane by reprobing. Each band in these sequences represents 3 fg of DNA complementary to the probe. Sequence data from mouse immunoglobulin heavy chain genes from several cell types are presented. The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.. MeSH Terms ...
more infohttp://genetics.mgh.harvard.edu/goodman/bac_bin/bac_bin.references/church.html
Detection of galectin-3 in tear fluid at disease states and immunohistochemical and lectin histochemical analysis in human...  Detection of galectin-3 in tear fluid at disease states and immunohistochemical and lectin histochemical analysis in human...
Progress in glycosciences has documented that biological information transfer not only exploits protein-protein and nucleic acid-protein interactions but also protein-carbohydrate recognition.1 Proteins involved in interaction with carbohydrates are known as lectins. Based on structural analysis of the carbohydrate recognition domains, animal lectins are currently classified into five categories: C type, I type, P type, galectins, and pentraxins.2 Cells as well as extracellular matrix molecules of normal and pathological corneas and conjunctivas in mammals are already known to contain glycans recognised by numerous plant lectins.3 4 The sugar receptors in these tissues have been demonstrated by employing labelled neoglycoligands.5 This experimental basis encourages us to further investigate expression of endogenous lectins on the eye surface. In this report, we focus on a member of the animal lectin family of the galectins. Mammalian galectins at present comprise nine proteins sharing the ...
more infohttp://bjo.bmj.com/content/85/11/1336
Absolute binding-free energies between standard RNA/DNA nucleobases and amino-acid sidechain analogs in different environments  Absolute binding-free energies between standard RNA/DNA nucleobases and amino-acid sidechain analogs in different environments
ABSTRACTDespite the great importance of nucleic acid-protein interactions in the cell, our understanding of their physico-chemical basis remains incomplete. In order to address this challenge, we have for the first time determined potentials of mean force and the associated absolute binding free ene
more infohttps://insights.ovid.com/nucar/201543020/00006178-201543020-00003
Address University Britisch Columbia  Address University Britisch Columbia
I would like the have the address, phone number and e-mail of the University ,of Britisch Columbia where I can order PCR primers. The University of British Columbia Nucleic Acid-Protein Service Unit Dr. J. B. Hobbs, Director Room 237 - Wesbrook Building 6174 University Blvd Vancouver, BC v6T 1Z3 CANADA Phone 604 822 6372 Fax: 604 822 5437 hobbs at unixg.ubc.ca Hope it helps!! C. S. Prakash Voice (334) 727 - 8023 Tuskegee University Fax (334) 727 - 8552 School of Agriculture Alt. Fax (334) 727 - 8493 Tuskegee, Alabama 36088 USA Email: Prakash at Acd.Tusk.Edu ...
more infohttp://www.bio.net/bionet/mm/methods/1995-November/035770.html
Design, synthesis, and evaluation of an isotopic labeling strategy for studying fatty acid-protein binding by NMR - Molecular...  Design, synthesis, and evaluation of an isotopic labeling strategy for studying fatty acid-protein binding by NMR - Molecular...
Here we describe the synthesis and evaluation of a new isotopic labeling strategy for fatty acids to be used as probes for studying ligand binding by NMR. We synthesized palmitic acid with carbons C-3 through C-16 perdeuterated, C-1 and C-2 with 13C atoms and hydrogens at C-2. Our strategy began with commerc
more infohttp://pubs.rsc.org/en/content/articlelanding/2008/mb/b800471d?iscitedby=True
The binding affinity of amino acid-protein: hydroxyproline binding site I on human serum albumin  The binding affinity of amino acid-protein: hydroxyproline binding site I on human serum albumin
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
more infohttps://www.growkudos.com/publications/10.1039%25252Fc2ob25967b/reader
IJMS  | Free Full-Text | Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid,...  IJMS | Free Full-Text | Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid,...
Clathrin, a three-legged triskelion composed of three clathrin heavy chains (CHCs) and three light chains (CLCs), plays a critical role in clathrin-mediated endocytosis (CME) in eukaryotic cells. In this study, the genes ZmCHC1 and ZmCHC2 encoding clathrin heavy chain in maize were cloned and characterized for the first time in monocots. ZmCHC1 encodes a 1693-amino acid-protein including 29 exons and 28 introns, and ZmCHC2 encodes a 1746-amino acid-protein including 28 exons and 27 introns. The high similarities of gene structure, protein sequences and 3D models among ZmCHC1, and Arabidopsis AtCHC1 and AtCHC2 suggest their similar functions in CME. ZmCHC1 gene is predominantly expressed in maize roots instead of ubiquitous expression of ZmCHC2. Consistent with a typical predicted salicylic acid (SA)-responsive element and four predicted ABA-responsive elements (ABREs) in the promoter sequence of ZmCHC1, the expression of ZmCHC1 instead of ZmCHC2 in maize roots is significantly up-regulated by SA or ABA,
more infohttp://www.mdpi.com/1422-0067/14/7/15179/htm
Mechanism of Fatty Acid Modulation of Calcium-Activated Potassium Chan by Alison L. Clarke  "Mechanism of Fatty Acid Modulation of Calcium-Activated Potassium Chan" by Alison L. Clarke
The purpose of this work was to determine whether the previously identified fatty acid activation of large conductance Ca2+-activated K+ (BK) channels from rabbit pulmonary artery smooth muscle cells was due to the direct interaction of the fatty acid with a site on the channel protein. If this was found to be the case, this study would also attempt to identify the site of fatty acid-protein interaction. Fatty acids released from membrane phospholipids by cellular phospholipases or available to the cell from the extracellular environment are important signaling molecules. Fatty acids can modulate the activity of a large number of molecules including protein kinases, phospholipases, adenylate and guanylate cyclases, G-proteins and ion channels. Fatty acids have also been shown to activate transcription of genes belonging to the steroid/thyroid superfamily of receptors. The actions of fatty acids on signal transduction pathways can be direct, whereby the fatty acid molecule itself is responsible for
more infohttp://escholarship.umassmed.edu/gsbs_diss/277/
SMART: SERPIN domain annotation  SMART: SERPIN domain annotation
Estrogen treatment of Xenopus frogs causes four mRNAs to become highly abundant in the liver. Three of these mRNAs have been previously identified as coding for vitellogenin, ferritin, and serum retinol binding protein. We show here that the fourth abundant liver messenger RNA comprises about 1500 nucleotides and codes for a 45-kDa secreted protein, designated Ep45. A clone complementary to Ep45 mRNA was isolated, and its identity was confirmed by hybridization selection of mRNA that translated in vitro into the Ep45 precursor. Nucleotide sequence analysis of the nearly full length cDNA revealed a total length of 1454 base pairs consisting of: 36 nucleotides of the 5' noncoding region, 1308 base pairs encoding an open reading frame of 436 amino acids, and 110 nucleotides of the 3' untranslated region. Ep45 mRNA may originate from as many as four closely spaced transcription start sites, which are 15 to 21 bases upstream of the first nucleotide of the cDNA clone. The Xenopus laevis genome appears ...
more infohttp://smart.embl.de/smart/do_annotation.pl?DOMAIN=SM00093
7-methylguanosine-capped green fluorescent protein mRNA/human eukaryotic initiation factor 4E@triethylene tetramine-substituted...  7-methylguanosine-capped green fluorescent protein mRNA/human eukaryotic initiation factor [email protected] tetramine-substituted...
Page contains details about 7-methylguanosine-capped green fluorescent protein mRNA/human eukaryotic initiation factor [email protected] tetramine-substituted polyamine nanoplexes . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
more infohttp://nano.nature.com/nano/GR-M9068701
Biological relevance and therapeutic potential of the hypusine modification system  - Zurich Open Repository and Archive  Biological relevance and therapeutic potential of the hypusine modification system - Zurich Open Repository and Archive
Hypusine modification of the eukaryotic initiation factor 5A (eIF-5A) is emerging as a crucial regulator in cancer, infections and inflammation. Although its contribution in translational regulation of proline-repeat-rich proteins has been sufficiently demonstrated, its biological role in higher eukaryotes remains poorly understood. To establish the hypusine modification system as a novel platform for therapeutic strategies, we aimed to investigate its functional relevance in mammals by generating and using a range of new knockout mouse models for the hypusine modifying enzymes deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH) as well as for the cancer-related isoform eIF-5A2. We uncovered that homozygous depletion of DHS or DOHH causes lethality in adult mice with different penetrance compared to haploinsufficiency. Network-based bioinformatic analysis of proline-repeat-rich proteins, which are putative eIF-5A targets, revealed that these proteins are ...
more infohttp://www.zora.uzh.ch/id/eprint/111316/
nip1 - Eukaryotic translation initiation factor 3 subunit C - Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission...  nip1 - Eukaryotic translation initiation factor 3 subunit C - Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission...
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
more infohttp://www.uniprot.org/uniprot/O14164
eif-3.F - Eukaryotic translation initiation factor 3 subunit F - Caenorhabditis elegans - eif-3.F gene & protein  eif-3.F - Eukaryotic translation initiation factor 3 subunit F - Caenorhabditis elegans - eif-3.F gene & protein
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
more infohttp://www.uniprot.org/uniprot/Q18967
Eukaryotic translation initiation factor 1 Proteins: Novus Biologicals  Eukaryotic translation initiation factor 1 Proteins: Novus Biologicals
Eukaryotic translation initiation factor 1 Proteins available through Novus Biologicals. Browse our Eukaryotic translation initiation factor 1 Protein catalog backed by our Guarantee+.
more infohttps://www.novusbio.com/proteins/eukaryotic-translation-initiation-factor-1