Leicester Research Archive: Minisatellite isoalleles can be distinguished by single-stranded conformational polymorphism...
We now show that minisatellite isoalleles can also be simply resolved by single-stranded conformational polymorphisms (SSCP) ... Minisatellite isoalleles can be distinguished by single-stranded conformational polymorphism analysis in agarose gels. ...https://lra.le.ac.uk/handle/2381/22341
Mutations of p53 gene can be detected in the plasma of patients with large bowel carcinoma. | Journal of Clinical Pathology
"Cold" single strand conformational polymorphism (SSCP) was performed on mini gels and silver stained. Abnormal bands were ...http://jcp.bmj.com/content/51/8/611
Detection of human herpes virus 8 DNA and sequence polymorphism in classical, epidemic, and iatrogenic Kaposi's sarcoma in...
We investigated polymorphism in the HHV-8 DNA using single-stranded conformational polymorphism (SSCP) analysis on the PCR ... Direct sequencing was performed on 5 selected cases and showed single base pair substitutions in all. The spectrum of mutations ... We report on the detection of HHV-8 DNA and sequence polymorphism in different clinicopathological subtypes of cutaneous KS ... Detection of human herpes virus 8 DNA and sequence polymorphism in classical, epidemic, and iatrogenic Kaposi's sarcoma in ...http://scholar.sun.ac.za/handle/10019.1/14052
Bio572: DNA Diagnostics
SSCP = Single Strand Conformational Polymorphism, and this is a slightly different approach to finding changes in DNA sequence ... During the studies that led to the development of single-stranded conformational polymorphism (SSCP) assays, Hyashi (1) noted ... analysis of single-strand conformation polymorphism (SSCP). The p27 gene was reverse transcribed and amplified by PCR and ... polymerase chain reaction-single strand conformation polymorphism) analysis on these variables.'. ...http://escience.ws/b572/L25/L25.htm
An association between lifespan and variation in insulin-like growth factor I receptor in sheep
Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was used to type IGF1R variation in 1,716 New ... polymerase chain reaction-single strand conformational polymorphism; sheep; Animals; Receptor, IGF Type 1; Gene Expression ... Polymorphism in the IGF1R gene has been associated with longevity in a number of species. Recently, 3 alleles of ovine IGF1R ...http://dspace.lincoln.ac.nz/handle/10182/8556
Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and...
"Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and ... Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and ... Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and ... was investigated by single-strand conformational polymorphism (SSCP) analysis and nucleotide sequencing. Forty-eight samples of ...https://www.deepdyve.com/lp/springer_journal/evaluation-of-the-genetic-variability-of-orchid-fleck-virus-by-single-F7LkWFBGFF
Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: comparison of single-strand...
... is still a frequently used genotyping method across different fields for the detection of single nucleotide polymorphisms (SNPs ... In contrast, SSCP and pyrosequencing of an unrelated single SNP (rs1835740:C > T), showed 94% concordance. Our data suggest ... Single-strand conformational polymorphism (SSCP) is still a frequently used genotyping method across different fields for the ... Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: comparison of single-strand ...https://bmcgenet.biomedcentral.com/articles/10.1186/1471-2156-15-80
Development of single-strand conformational polymorphism to screen for mutations in hotspot regions of beta-globin gene of...
Development of single-strand conformational polymorphism to screen for mutations in hotspot regions of beta-globin gene of beta ... Development of single-strand conformational polymorphism to screen for mutations in hotspot regions of beta-globin gene of beta ... Single strand conformational polymorphism (SSCP) technique was developed as an adjunct for the previously developed allele- ...http://repository.kln.ac.lk/handle/123456789/2226
Endotoxin-tolerant Mice Have Mutations in Toll-like Receptor 4 (Tlr4) | JEM
Single-strand Conformational Polymorphism Analysis.. To detect single nucleotide changes in alleles of different inbred mouse ... The nucleotide sequences of polymorphisms detected by single-strand conformational polymorphism (SSCP) analysis were determined ... single-strand conformational polymorphism. SSLP. simple sequence length repeat. STS. sequence-tagged site. TIR. Toll IL-1 ... First-strand cDNA synthesis was carried out using 2 μg of total RNA, 100 ng of random hexamers, and 200 U of Moloney murine ...http://jem.rupress.org/content/189/4/615
Distinct genetic profiles in colorectal tumors with or without the CpG island methylator phenotype | PNAS
single-stranded conformational polymorphisms;. LOH,. loss of heterozygosity;. MSP,. methylation-specific PCR. ... Single-Stranded Conformational Polymorphisms (SSCP) and Sequencing.. PCR was performed as described previously by using primers ... Four additional cases had intragenic p53 LOH (determined by using polymorphisms in exon 2, 3, or 4 of the gene) but no ... Integrative analysis of single-cell genomics data by coupled nonnegative matrix factorizations ...http://www.pnas.org/content/97/2/710?ijkey=c75cf64d82fe6cafc5fce373900194b130cf4f07&keytype2=tf_ipsecsha
RNA Single-Stranded Conformational Polymorphism.. To determine whether modifications in the ATG of the uORF in the 5′-UTR of ... 1992) Screening for mutations by single strand conformation polymorphism (rSSCP): Comparison with DNA-SSCP. Nucleic Acid Res 20 ... To determine whether mutation of the upstream ATG influences the secondary structure of the mRNA, single-stranded radiolabeled ... As shown in Fig.8, RNA produced from the native 5′-UTR of CRHR1 migrated as a single band. A similar mobility was observed for ...http://molpharm.aspetjournals.org/content/59/3/485
High resolution melting curve analysis enables rapid and reliable detection of G6PD variants in heterozygous females |...
... single strand conformational polymorphism (SSCP) etc. are either costly or technically cumbersome and requires the use of ... Sensitivity and specificity of single-nucleotide polymorphism scanning by high-resolution melting analysis. Clin Chem. 2004;50( ... n UDP-glucuronosyltransferase 1A1 promoter polymorphism in Nigerian neonates. Pediatr. Res. 2008;63(1):109.CrossRefPubMedGoogle ... The frequency of UDP-glucuronosyltransferase 1A1 promoter region (TA) 7 polymorphism in newborns and it's relation with ...https://link.springer.com/article/10.1186%2Fs12863-018-0664-1
Table 3 - Molecular Approaches to Diagnosing and Managing Infectious Diseases: Practicality and Costs - Volume 7, Number 2...
gAbbreviations not defined in text: RFLP, restriction fragment length polymorphism; SSCP, single-stranded conformational ... Recurrent epidemics caused by a single strain of erythromycin-resistant Staphylococcus aureus: The importance of molecular ... polymorphism; LIPA, line probe assay; TK, thymidine kinase; RT, reverse transcriptase; PROT, protease. ...https://wwwnc.cdc.gov/eid/article/7/2/70-0312-t3
Genetic Analysis of the β Subunit of the Epithelial Na+ Channel in Essential Hypertension | Hypertension
polymorphism, single-stranded conformational. *oocytes. *hypertension, essential. The ENaC constitutes the rate-limiting step ... Uracyl-containing single-strand DNA was precipitated using 5% PEG 6000 and 0.95 mol/L ammonium acetate and then purified using ... β Single-strand DNA (500 ng) and 10 pmol phosphorylated mutagenic primer were denatured together for 10 minutes at 95°C, slowly ... That single purpose drives all we do. The need for our work is beyond question. Find Out More about the American Heart ...http://hyper.ahajournals.org/content/32/1/129
MSI Testing and IHC | China-Mainland | Sigma-Aldrich
Single-strand conformational polymorphism. *Denaturing gradient gel electrophoresis analysis. *DNA sequencing. Alternative ... Tumor DNA strands and normal DNA are compared in neighboring lanes. Tumors are considered MSI-H (high instability) if two or ... Strand M, Prolla TA, Liskay RM, et al. Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA ... Testing for a single point mutation (rather than sequencing the entire gene) makes BRAF mutation analysis economically ...https://www.sigmaaldrich.com/china-mainland/zh/technical-documents/articles/white-papers/msi-testing-and-ihc.html
β-Catenin Mutations and Protein Accumulation in All Hepatoblastomas Examined from B6C3F1 Mice Treated with Anthraquinone or...
The abbreviations used are: APC, adenomatous polyposis coli; SSCP, single-strand conformational polymorphism. ... The samples were sectioned with a cryostat, and a single section was quick stained to identify the hepatoblastomas. Then, the ...http://cancerres.aacrjournals.org/content/60/11/2864.long
CASP6 | Cancer Genetics Web
Single-Stranded Conformational Polymorphism. *Up-Regulation. *Breast Cancer. *Disease Progression. *Oligonucleotide Array ... and single nucleotide polymorphism (SNP) array copy number analysis.. METHODS: We used conventional cytogenetics, FISH, and ... potentially functional single nucleotide polymorphisms (SNPs) located in the microRNA binding sites of the 3' untranslational ... which was generated by fusing a HER2-specific single-chain Ab, a single-chain Pseudomonas exotoxin A and an active caspase-6 ...http://www.cancerindex.org/geneweb/CASP6.htm
Molecular Genetic Study of Finns With Hypoalphalipoproteinemia and Hyperalphalipoproteinemia | Arteriosclerosis, Thrombosis,...
single-strand conformational polymorphism. Acknowledgments. This study was supported by grants from the Medical Council of the ... was used to generate single-stranded DNA. Strong DNA secondary structures in the G-C-rich area of exon 6 and compressions in ... That single purpose drives all we do. The need for our work is beyond question. Find Out More about the American Heart ... Assays for Specific Mutations and Polymorphisms. A PCR-based assay using the principle of a mismatch primer in the detection of ...http://atvb.ahajournals.org/content/18/4/591
A Common Mutation in the Lipoprotein Lipase Gene Promoter, −93T/G, Is Associated With Lower Plasma Triglyceride Levels and...
Abstract Single-strand conformational polymorphism analysis of the lipoprotein lipase promoter identified a T→G transition at ... Gudnason V, Mak YT, Betteridge J, McCarthy SN, Humphries S. Use of the single-strand conformational polymorphism method to ... and single-stranded DNA-binding proteins. Mol Cell Biol. 1995;15:517-523. ... thus allowing the purification of single-stranded DNA with streptavidin-coated beads (Dynal) and sequencing according to the ...http://atvb.ahajournals.org/content/17/10/1969
Heterozygosity in CTLA-4 gene and severe preeclampsia.
Polymorphism, Genetic / genetics*. Polymorphism, Single-Stranded Conformational. Pre-Eclampsia / genetics*. Pregnancy. Chemical ... A49G polymorphism in position 49 of exon-1 of the CTLA-4 gene was studied by the polymerase chain reaction-single-strand ... Whether the observed association results from linkage imbalance with other loci on chromosome 2 or other polymorphisms of the ... Therefore, the frequency of CTLA-4 A49G polymorphism was investigated in severe preeclampsia. PATIENTS AND METHODS: Genomic DNA ...http://www.biomedsearch.com/nih/Heterozygosity-in-CTLA-4-gene/15617700.html
Lymph Node Metastasis Is Associated with Allelic Loss on Chromosome 13q12-13 in Esophageal Squamous Cell Carcinoma1 | Cancer...
... single-strand conformational polymorphism; RT-PCR, reverse transcription-PCR; pRB, retinoblastoma protein; TFII H, ... a mutation of the gene through all coding exons and exon-intron junctions by PCR-single-strand conformational polymorphism ... The high sensitivity of our methods was demonstrated by the fact that we detected seven polymorphisms with single-base ... Six of 60 tumors (10%) revealed LOH at only a single locus. Four of 60 tumors (6.7%) showed LOH at all informative loci, which ...http://cancerres.aacrjournals.org/content/59/15/3724.full
Treesearch - scientific articles written or published by the U.S. Forest Service
Using single strand conformational polymorphisms (SSCP) to identify Phytophthora species in Oregon forests affected by sudden ...https://www.fs.usda.gov/treesearch/search?keywords=%22Phytophthora+species%22
... single-strand conformational polymorphism; TGGE, temperature gradient gel electrophoresis; RT-PCR, reverse transcription-PCR; 5 ... single nucleotide polymorphism; HMG, high mobility group; GSK-3β, glycogen synthase kinase 3 beta; G3PDH, glyceraldehyde 3 ... 3a⇓ .) For example, in RCC with type I, both nested PCR1 and nested PCR2 showed only a single band corresponding to 75 bp, ... RCC with type IV showed only a single band in nested PCR1, whereas there was no band detectable in nested PCR2, suggesting the ...http://clincancerres.aacrjournals.org/content/9/6/2121
... single-strand conformational polymorphism. ...http://clincancerres.aacrjournals.org/content/5/12/4053
Reduced MeCP2 expression is frequent in autism frontal cortex and correlates with aberrant MECP2 promoter methylation
To test for allelic heterogeneity, the same samples were tested with single strand conformational polymorphism (SSCP) using PCR ... Single Strand Conformation Polymorphism (SSCP) analysis. The 175 bp PCR product used for HpyCH4III genotyping was used for SSCP ... One female autism sample, AUT 1638, was heterozygous for a silent polymorphism in exon 4 (c.1035 A,G). This polymorphism has ... A single fragile X frontal cortex sample showed normal MeCP2. These results suggest that most, but not all, autism spectrum ...http://pubmedcentralcanada.ca/pmcc/articles/PMC1866172/?lang=en-ca