Phosphorylation of the c-Fos transrepression domain by mitogen-activated protein kinase and 90-kDa ribosomal S6 kinase
... as opposed to singly phosphorylated peptides. This study suggests a role for nuclear RSK and MAP kinase in modulating newly ... The phosphorylation sites identified for RSK (Ser-362) and MAP kinase (Ser-374) are in the transrepression domain. Cooperative ... MAP kinase), contribute to the serum-induced phosphorylation of c-Fos. The major phosphopeptides derived from biosynthetically ... phosphorylation at these sites by both enzymes was observed in vitro and reflected in vivo by the predominance of the peptide ...
Application of chemical selective cleavage methods to analyze post-translational modification in proteins
... one for the carboxyl side of asparaginyl peptide bonds and another for the amino side of seryl/threonyl peptide bonds have been ... one for the carboxyl side of aspartyl peptide bonds, ... reactions on these modifications change the peptide mapping ... one for the carboxyl side of asparaginyl peptide bonds and another for the amino side of seryl/threonyl peptide bonds have been ... Additionally, these reactions simultaneously react on several post-translationally modified groups in peptides or proteins. The ...
Functional characterization and high-throughput proteomic analysis of interrupted genes in the archaeon Sulfolobus solfataricus
Peptide Mapping * Proteome / analysis* * Proteomics / methods* * Pseudogenes * Reverse Transcriptase Polymerase Chain Reaction ... In addition, the sequence of the peptides determined by LCMSMS and experiments of in vitro translation allows us to identify a ...
Limited proteolysis as a probe for arrested conformational maturation of delta F508 CFTR
Peptide Mapping * Phenylalanine * Protein Conformation* * Recombinant Proteins / chemistry * Recombinant Proteins / isolation ...
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Understanding the pathway and kinetics of aspartic acid isomerization in peptide mapping methods for monoclonal antibodies. ... of a Quantitative Method for Monitoring Aspartate Isomerization of a Monoclonal Antibody by Focused Peptide Mapping. Cao M, Mo ...
Art v 1 IgE epitopes of patients and humanized mice are conformational
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Slc15a4, AP-3, and Hermansky-Pudlak syndrome proteins are required for Toll-like receptor signaling in plasmacytoid dendritic...
The feeble phenotype was mapped to a mutation in Slc15a4, which encodes the peptide/histidine transporter 1 (PHT1) and has not ...
Integrin regulation of cell signalling and motility
Thus integrins modulate the efficiency of the Erk (extracellular-signal-regulated kinase)/MAP kinase (mitogen-activated protein ... Intracellular Signaling Peptides and Proteins * MAP Kinase Signaling System * Mitogen-Activated Protein Kinase 3 / metabolism ... Thus integrins modulate the efficiency of the Erk (extracellular-signal-regulated kinase)/MAP kinase (mitogen-activated protein ...
Autoradiographic visualization of the receptor subclasses for vasoactive intestinal polypeptide (VIP) in rat brain
A cyclic peptide (RO 25-1553) and a secretion analogue ([R16 … ... Their messenger RNAs have been mapped in rat brain by in situ ... The iodinated peptides retained the high affinity and selectivity of the unlabelled peptides and were used for the mapping of ... Their messenger RNAs have been mapped in rat brain by in situ hybridization. A cyclic peptide (RO 25-1553) and a secretion ... analogue ([R16]chicken secretin) were identified as selective agonist peptides for the VIP2- and VIP1 receptors, respectively. ...
The cardiovascular responses to mu opioid agonist and antagonist in conscious normal and obese rats
Peptides. 2006 Jun;27(6):1520-6. doi: 10.1016/j.peptides.2005.10.012. Epub 2005 Nov 15. ... The heart rate responses paralleled the MAP responses. CTAP, the mu antagonist, paradoxically increased the MAP in both control ... DOI: 10.1016/j.peptides.2005.10.012 Abstract Beta-endorphin decreases blood pressure in normal rats but increases blood ... Rats were also implanted with telemetry transmitters and intracerebroventricular (ICV) cannulas for recording and peptide ...
Evaluation of novel cyclic analogues of apelin
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Immunoproteomics using polyclonal antibodies and stable isotope-labeled affinity-purified recombinant proteins
Epitope mapping revealed that a majority of the polyclonal antibodies recognized multiple linear epitopes, and based on these ... of more than 40 protein targets showed that approximately half of the antibodies enriched at least one functional peptide ... antibodies generated via immunization of recombinant protein fragments could be used for the enrichment of target peptides to ... results, a semi-automated method was developed for peptide enrichment using polyclonal antibodies immobilized on protein A- ...
Signaling and circuitry of multiple MAPK pathways revealed by a matrix of global gene expression profiles
MAP Kinase Signaling System* * Mating Factor * Mitogen-Activated Protein Kinases / metabolism * Multigene Family ... Peptides / pharmacology * Peptides / physiology * Pheromones * Protein Kinase C / metabolism * Repressor Proteins* * ...
Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes
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Monoclonal Antibody 2C6 Targets a Cross-Clade Conformational Epitope in gp41 with Highly Active Antibody-Dependent Cell...
Peptide library scanning showed consistent binding in this region but to only a single peptide. Lack of overlapping peptide ... When we mapped these amino acids on the trimerized structure, they spanned across oligomers, supporting the notion that the ... in the overlapping region of the previously identified peptide. Additional amino acids at sites 524 and 579 were also ...
Genome sequencing reveals complex secondary metabolome in the marine actinomycete Salinispora tropica
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A genome-wide association meta-analysis of plasma Aβ peptides concentrations in the elderly. Chouraki V, et al. Mol Psychiatry ... Genes and mapped phenotypes. Search database. All Databases. Assembly. Biocollections. BioProject. BioSample. Books. ClinVar. ... A genome-wide association meta-analysis of plasma Aβ peptides concentrations in the elderly.. EBI GWAS ...
N-linked glycan structures of mouse interferon-beta produced by Bombyx mori larvae
... including the secretion signal peptide coding region under control of the polyhedrin promoter, was introduced into Bombyx mori ... The structures of the PA-sugar chains were identified by a combination of two-dimensional PA-sugar chain mapping, MS analysis, ... The full-length mouse interferon-beta (mIFN-beta) cDNA, including the secretion signal peptide coding region under control of ...
Dynamic interactions of proteins in complex networks: a more structured view
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Systematic Discovery of Short Linear Motifs Decodes Calcineurin Phosphatase Signaling
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Docking interactions in protein kinase and phosphatase networks
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4. Genome-wide PSR1 binding profiling was performed with polyclonal antibody against PSR1 peptide A region and PSR1 peptide B ... The comprehensive maps of functional chromatin signatures in a major clade of eukaryotic life and the discovery of a central ...
Fibril structure of amyloid-β(1-42) by cryo-electron microscopy
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Sutural cataract associated with a mutation in the ferritin light chain gene (FTL) in a family of Indian origin
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Identification of tightly regulated groups of genes during Drosophila melanogaster embryogenesis
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Genes and mapped phenotypes. Search database. All Databases. Assembly. Biocollections. BioProject. BioSample. Books. ClinVar. ...
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Genes and mapped phenotypes. Search database. All Databases. Assembly. Biocollections. BioProject. BioSample. Books. ClinVar. ... Find Recombinant Proteins and Peptides *NITE Biological Resource Center. * Human cDNA clones used in NEDO Project / AK023560 ...
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