This makes PC12 cells useful as a model system for neuronal differentiation and neurosecretion. Treatment of PC12 cells with ... been studied using this cell line to understand their effects on PC12 cells These types of studies show that use of PC12 cell ... "PC12 cells" [1] Ras function was partially elucidated using PC12 cell line [2], from section in Cancer Medicine textbook ... PC12 cells treated for 10-14 days with nerve growth factor had no release of vesicles from the cell body which indicates the ...
PC12 cells were transfected with vector, wild type or mutant Ngb DNA (1.0 μg/106 cell) for 48 h and treated with H2O2 (0.1mM) ... PC12 cells were transfected with 0.5 μg, 1.0 μg and 2.0 μg/106 cells of Ngb, Ngb mutant or vector DNA for 48 h. Following ... PC12 cells were transfected with Ng siRNA or control siRNA for 48 h. Following transfection, the cells were treated with H2O2 ( ... Ngb protein expression and cell viability in PC12 cells after transfection of Ngb siRNA and treatment of H2O2 ...
... Michael Holloway mhollowa at ccnova.sunysb.edu Tue Jul 21 18:08:00 EST 1992 *Previous ... Ive learned then that the upshot is that successfully keeping PC12 cells going relies entirely on a qualitative feel for the ... This is of particular importance to me now because of the results Ive gotten in preliminary observations of PC12 cells ... Apparently, the way cell density is followed on a practical basis is to split the cells when a certain degree of confluence is ...
Gelsolin overexpression enhances neurite outgrowth in PC12 cells.. Furnish EJ1, Zhou W, Cunningham CC, Kas JA, Schmidt CE. ... Cells that were genetically modified to overexpress gelsolin have longer neurites and a greater neurite motility rate compared ... One actin accessory protein, gelsolin, is involved with remodeling the cytoskeleton, although its role in cell motility is ...
Students will explore the signaling pathways that lead to neuronal differentiation of a cultured cell line. ... Students will explore the signaling pathways that lead to neuronal differentiation of a cultured cell line. ...
... Rae Rae Fri May 27 16:50:10 EST 1994 *Previous message: How to differentiate PC12 ... I intended to differentiate PC12 cell with NGF. I added NGF in culture , medium containing 5% horse serum/fetal bovine serum. ... Another problem might be your PC12 cell line- if theyve been carried too long and allowed to overgrow before splitting, then ... However, only ,small part of cells differentiated. How can I overcome this problem ? ,Is it necessary to change the medium to ...
PC12 cells synthesize catecholamines (primarily dopamine) and acetylcholine (ACh), store each in different granules and exhibit ... PC12 is a clonal line of rat pheochromocytoma (7). ... PC12 is a clonal line of rat pheochromocytoma (7). PC12 cells ... Howard B.D., Melega W.P. (1981) Acetylcholine Metabolism in PC12, A Clonal Cell Line on Secretory Cells. In: Pepeu G., Ladinsky ... PC12 Cell Synaptic Vesicle Incubation Buffer ATPase Inhibitor Chromaffin Granule These keywords were added by machine and not ...
... Ji Jia,1 Lei Zhang,2 Xiaolei Shi,3 Mingchun Wu,4 ... Ji Jia, Lei Zhang, Xiaolei Shi, et al., "SOD2 Mediates Amifostine-Induced Protection against Glutamate in PC12 Cells," ...
Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter, Spatial and ... pc12 cells include Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell ... Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact, In Vivo Single-Molecule ... Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5, Experimental Methods for Testing the Effects of ...
... dimeric proteins distributed widely among eukaryotic cells. This protein family binds to a variety of proteins in a ... PC12 Cell Tyrosine Hydroxylase Sepharose Bead Signal Transduction Kinase Major Ligand These keywords were added by machine and ... Ichimura T., Itagaki C., Wakamiya A., Isobe T. (2002) Interaction of Tyrosine Hydroxylase and 14-3-3 Proteins in PC12 Cells. In ... Interaction of Tyrosine Hydroxylase and 14-3-3 Proteins in PC12 Cells. ...
... cells treated with 6-OHDA (50 μM) for 1 hour, Group 2: cells treated with 6-OHDA (150 μM) for 1 hour, Group 3: cells treated ... Therefore, preexposure of PC12 cells to low 6-OHDA concentration can protect against subsequent toxic insults of 6-OHDA and OA ... Oleanolic Acid Enhances the Beneficial Effects of Preconditioning on PC12 Cells. Babongile C. Ndlovu, Willie M. U. Daniels, and ... In preconditioned cells, we found that cell viability remained high following exposure to 6-OHDA (150 μM). OA treatment ...
Cell survival in both untreated and nerve growth factor (NGF)-treated PC12 cells was inhibited by down-regulation of SOD1, with ... Down-regulation of copper/zinc superoxide dismutase causes apoptotic death in PC12 neuronal cells. C M Troy and M L Shelanski ... We have approached this problem by specifically inhibiting the synthesis of SOD1 in cultured PC12 cells with antisense ... Down-regulation of copper/zinc superoxide dismutase causes apoptotic death in PC12 neuronal cells ...
IMAGE: Protective effect of rAAV/ABAD-DP on H2O2-mediated changes in morphology of PC12 cells. ABAD: Aβ-binding alcohol ... Article: " rAAV/ABAD-DP-6His attenuates oxidative stress-induced injury of PC12 cells," by Mingyue Jia1, Mingyu Wang2, Yi Yang1 ... rAAV/ABAD-DP-6His attenuates oxidative stress induced injury of PC12 cells. Neural Regeneration Research ... rAAV/ABAD-DP-6His attenuates oxidative stress induced injury of PC12 cells ...
This agent induced a dose- and time-dependent cell death in neuronal PC12 cells. Cells treated with this agent showed condensed ... PC12 cells induced to differentiate with nerve growth factor were used to study the neurotoxicity of 25-OH-cholesterol. ... This agent induced a dose- and time-dependent cell death in neuronal PC12 cells. Cells treated with this agent showed condensed ... In contrast to observations made in other cell types, whole-cell patch clamp recording of neuronal PC12 cells revealed that ...
Using PC12 cell model, we showed that Glu and Hcy provoked a huge amount of reactive oxygen species (ROS) production, causing ... the damages caused by Glu and Hcy to PC12 cells can be alleviated by the potent antioxidant ATX. ... We hypothesize that Glu and Hcy could damage neuronal cells, while astaxanthin (ATX) could be beneficial to alleviate the ... Source of Cell Line. The cell line PC12 cells (BCRC 6008), originating from the pheochromocytoma on the rat adrenal gland, was ...
... and percent of bipolar cells and node/primary neurite ratios were used to assess the growth and complexity of PC12 cells ... We observed that the oligomeric form of insulin impaired the growth and complexity of PC12 cells compared to other forms. ... our data suggest that the lower surface tension of oligomers and their perturbation affects the morphology of PC12 cells, ... toxicity of the different forms of insulin we monitored morphological alterations of the differentiated neuron-like PC12 cells ...
... the effects of ablated S100A1 expression on neurite organization and microtubule/tubulin levels in PC12 cells were examin ... PC12 Cells. Rats. Recombinant Proteins / metabolism. S100 Proteins. Signal Transduction. Subcellular Fractions / metabolism. ... In summary, ablation of S100A1 expression in PC12 cells results in increased tubulin levels, altered neurite organization, and ... the effects of ablated S100A1 expression on neurite organization and microtubule/tubulin levels in PC12 cells were examined. A ...
PC12 cells have caveolae that contain TrkA. Other Titles: Caveolae-disrupting drugs inhibit nerve growth factor-induced, but ... Here we demonstrate the presence of caveolae in PC12 cells and their involvement in NGF signaling. Our results showed the ... In contrast, pretreatment of PC12 cells with these drugs inhibited the NGF- but not the epidermal growth factor-lnduced MAPK ... Nerve growth factor (NGF) induces survival and differentiation of the neural crest-derived PC12 cell line. Caveolae are ...
... after 3 days of NGF treatment and a suppression of neurite formation upon Praja1 overexpression in stably transfected PC12 cell ... Here, we investigate its involvement in the nerve growth factor (NGF)-induced differentiation of rat pheochromocytoma (PC12) ... cells. Praja1 co-localizes with cytoskeleton components and the neurotrophin receptor interacting MAGE homologue (NRAGE). We ...
Ahnert-Hilger, G.; Gratzl, Manfred (1987): Further Characterization of Dopamine Release by Permeabilized PC 12 Cells. In: ... Rat pheochromocytoma cells (PC 12) permeabilized with staphylococcal α-toxin release [3H]dopamine after addition of micromolar ... dopamine release from digitonin-permeabilized PC 12 cells. Permeabilization with α-toxin or digitonin and stimulation of the ... OH groups are not necessary for exocytosis in permeabilized PC 12 cells. ...
... on cell survival and mitochondria functioning in intact cells under conditions of undergoing cell divisions. Because after 48h ... on cell survival and mitochondria functioning in intact cells under conditions of undergoing cell divisions. Because after 48h ... Therefore, we applied inducible PC12 cell model of HD to determine the relationship between the effect of expression of wild ... Therefore, we applied inducible PC12 cell model of HD to determine the relationship between the effect of expression of wild ...
Cell Culture and Tissue Engineering. Differentiation of PC12 cells in three-dimensional collagen sponges with micropatterned ... Limiting cell aggregation during mesenchymal stem cell expansion on microcarriers Next article in issue: Limiting cell ... PC12 cells were cultured in the NGF-micropatterned collagen sponges and showed micropatterned neurite outgrowth. The neurite ... The micropattern of the NGF and the neurite network of the PC12 cells can be manipulated by controlling the micropattern of the ...
... of the cell-bound 125I-NGF was found in the nuclear pellet after cell fractionation if the cells had been kept previously in ... PC12 cells were grown as monolayer cultures in medium supplemented with serum in the presence of 125I-NGF. EM autoradiography ... Internalization of nerve growth factor by pheochromocytoma PC12 cells: absence of transfer to the nucleus. H Rohrer, T Schafer ... The ultrastructure of PC12 cells grown under such conditions, however, revealed signs of varying degrees of damage. ...
The majority of the derivatives exhibited strong protective effects on PC12 (PC12 rat pheochromocytoma) cells exposed to H2O2, ... in PC12 cells. Inhibition of GCLC and HO-1 by specific inhibitors, l-buthionine-S-sulfoximine (BSO) and zinc protoporphyrin ( ... Rat PC12 cells were obtained from the Institute of Biochemistry and Cell Biology, SIBS, CAS. PC12 cells were cultured in DMEM ... Figure 1. Chalcone derivatives protected PC12 cells from H2O2-induced oxidative injury. PC12 cells were seeded in a 96-well ...
Treatment of PC12 cells with clinically applicable concentrations of L-DOPA (25-100 microM) induced cell death via a mechanism ... Induction of apoptosis in catecholaminergic PC12 cells by L-DOPA. Implications for the treatment of Parkinsons disease.. G ... L-DOPA-induced apoptosis was cell and drug-type specific. Toxicity is an intrinsic property of the drug molecule since it was ... Our finding that L-DOPA-induced cell death in vitro occurs via apoptosis explains the lack of evidence supporting its toxicity ...