... using patch-clamp techniques and fluorescent Ca2+ imaging [after electrical field stimulation (EFS) and acetylcholine (ACh)] in ...

This effect was determined by using whole-cell patch clamp technique and mediated by pharmacologically isolated AMPA/kainate ... Techniques like functional magnetic resonance imaging (fMRI) to display brain activity based on blood oxygenation difference ... A study [44] carried out on mouse visual cortex and employing the double photon technique disclosed that a single dendritic ... In the present investigation, through electrophysiological techniques, we examined the effect of sound on the simultaneously ...

... systems which are otherwise difficult to approach using conventional electrophysiological techniques, like patch clamp.". Dr. ... "SSM-based electrophysiology is a suitable technique to study bacterial ion-translocating membrane- ...

Within this research we looked into ET receptor expression in DRG and using the patch clamp technique the effects of ET-1 on ...

Specifically, by applying patch clamp techniques (whole cell voltage clamp and single channel in inside-out patches) we ... Specifically, by applying patch clamp techniques (whole cell voltage clamp and single channel in inside-out patches) we ... Specifically, by applying patch clamp techniques (whole cell voltage clamp and single channel in inside-out patches) we ... Specifically, by applying patch clamp techniques (whole cell voltage clamp and single channel in inside-out patches) we ...

1. Perforated patch-clamp recordings were made from the three major classes of hippocampal neurons in conventional in vitro ... Combining birth-dating techniques with transmitter immunocytochemistry revealed that it was the position in the birth order and ... 03/06/92 , Perforated patch-clamp analysis of the passive membrane properties of three classes of hippocampal neurons. Spruston ... This technique provided experimental estimates of passive membrane properties (input resistance, RN, and membrane time constant ...

Methods: Experiments were performed on enzymatically isolated rat ventricular myocytes by the whole cell patch clamp technique ...

... on the development of the patch clamp technique, which allowed us to record currents flowing through individual ion channels ... It is large enough that quantitative biophysical techniques, such as voltage clamp, Ca++ fluorimetry, and Ca++-ions uncaging ... and I will explain how the availability of a new sensitive recording technique led to the discovery of a multitude of ion ...

Cardiac cellular electrophysiology, voltage clamp, and patch clamp. / Chen, Yi J.; Chen, Yao Chang; Chen, Shih A. et al. In: ... Chen, Y. J., Chen, Y. C., Chen, S. A., & Lin, C. I. (2009). Cardiac cellular electrophysiology, voltage clamp, and patch clamp ... Chen, YJ, Chen, YC, Chen, SA & Lin, CI 2009, Cardiac cellular electrophysiology, voltage clamp, and patch clamp, Acta ... Cardiac cellular electrophysiology, voltage clamp, and patch clamp. In: Acta Cardiologica Sinica. 2009 ; Vol. 25, No. 2. pp. 59 ...

Whole cell patch-clamp recording technique was used to evaluate the effects of midazolam (0.1 to 100micrometer) on outward K+ ...

293 cells using whole-cell patch-clamp technique. It was found that HEK 293 cells stably expressing human Kir2.1 gene had a ... 293 cells using whole-cell patch-clamp technique. It was found that HEK 293 cells stably expressing human Kir2.1 gene had a ... Action potential waveform clamp revealed two components of outward currents; one was immediately elicited and then rapidly ... Action potential waveform clamp revealed two components of outward currents; one was immediately elicited and then rapidly ...

... using electrophysiological patch clamp techniques to record isolated ion currents from acutely isolated, adult rat brain, ... Our strategy includes patch clamp recording that addresses the technical challenges inherent in studying modulation of channels ... A) The patch pipette did not contain ATP. (B) The patch pipette contained 4 mM ATP. Inset in A: I-V relationship measured at ... the whole cell or single channel patch-clamp level, has allowed us to answer several questions not previously investigated in ...

... was studied in cultured rat trigeminal ganglion neurons using whole-cell patch-clamp recording techniques [1]. ...

Here, we used patch clamp electrophysiology to examine the effects of alcohol dependence on the CRF-modulation of rat CeA ... of evoked EPSC in nNOS interneurons using whole cell recording techniques. Results showed that there were fewer nNOS- ... Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: corticotropin releasing factor (CRF); patch-clamp ... Importantly, patch-clamp recordings revealed reduction in mEPSC frequency, indicating diminished global excitatory input. In ...

Electrophysiological measurements using the whole cell version of the patch-clamp technique were made simultaneously with [Ca2+ ... Electrophysiological measurements using the whole cell version of the patch-clamp technique were made simultaneously with [Ca2+ ... Electrophysiological measurements using the whole cell version of the patch-clamp technique were made simultaneously with [Ca2+ ... Electrophysiological measurements using the whole cell version of the patch-clamp technique were made simultaneously with [Ca2+ ...

... used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, ... Techniques Used: Construct, Patch Clamp, Activation Assay. Figure Legend Snippet: Localization of Nav1.5 WT and mutants ... Representative current traces and their biophysical properties measured via the patch clamp approach in HEK293 cells ... Representative current traces and their biophysical properties measured via the patch clamp approach in HEK293 cells ...

My project combined the patch-clamp electrophysiology expertise of the Porter laboratory with my expertise in the behavioral ... There I developed skills in several rodent behavioral techniques and was a part of world class behavioral neuroscience research ...

Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch ... Jackson MB (1992) Cable analysis with the whole-cell patch clamp. Theory and experiment. Biophys J 61:756-66 [Journal] [PubMed] ... Sakmann B, Neher E (1995) Geometric parameters of pipettes and membrane patches Single Channel Recording, Sakmann B:Neher E, ed ... Cohen D, Yarom Y (1998) Patches of synchronized activity in the cerebellar cortex evoked by mossy-fiber stimulation: ...

Using the perforated patch clamp technique (22) to record from single mouse β-cells bathed in 10 mmol/l glucose, we found that ... Using the perforated patch clamp technique, we found that insulin (1-600 nmol/l) but not IGF-1 (100 nmol/l) reversibly ... Using the perforated patch clamp technique (22), isolated β-cells were identified by their size and characteristic patterns of ... In cell-attached patches, single ATP-sensitive K+ (KATP) channels were activated by bath-applied insulin and subsequently ...

... and his group were excellent at patch clamping, a technique to look at ion channels in single cells. I wanted to stay in CF ... to this day was actually seeing the important chloride channel responsible for the CF defect popping away under a patch clamp ...

The loose-patch voltage clamp technique was used to measure the density and distribution of NaChs on muscle fibers regenerating ...

Usng the whole-cell patch-clamp technique, we have found that activation of group III mGluRs with L-AP4 inhibits peak calcium ... 1. Calcium currents in rabbit area postrema neurons were studied with the perforated patch-clamp technique. Experimental ... on voltage-gated calcium channels using standard patch-clamp techniques and on the process of exocytosis and endocytosis in ... Whole cell patch-clamp studies have reported that depolarization-induced calcium influx in aortic baroreceptor cell bodies is ...

... providing a tight electrical junction for solid-state patch clamping. In addition to significant efforts at engineering ... To accomplish this, we employ: precise growth techniques such as chemical vapor deposition and molecular beam epitaxy; ... My long history with nano- and microfabrication techniques and their interactions with biological constructs provide the skills ... I develop new, higher performance electrodes and advanced techniques to study material structure-property relationships during ...

The effects of AELL on mouse ASM tension were studied using force measuring and patch-clamp techniques. It was found that AELL ... The effects of AELL on mouse ASM tension were studied using force measuring and patch-clamp techniques. ... the channel-mediated currents were measured with the whole-cell patch-clamp technique. As shown in Fig. 3a, the currents were ... Whole-cell Ba2+ currents through VDCC were measured using an EPC-10 patch-clamp amplifier (HEKA, Lambrecht, Germany). The ...

It was held on the rim with clamps, instead of glue, and could be removed to replace or patch the separate inner tube.[2] ... Three main techniques for attaching a bicycle tire to a bicycle rim have been developed: clincher, wired and tubular.[8] ... If you were to pump your rear tire up to 100 psi, then your contact patch would be exactly one square inch in area. This is ... An advantage of this system is that the inner tube can be easily accessed for a patch repair or replacement of the tube. ...

b,Methods:,/b, The whole cell patch clamp technique was used to record action potentials, the effective refractory period (ERP ...

jones_sue_885_x_432.png University Senior Lecturer, Department of Physiology, Development and Neuroscience Sue Jones studies glutamatergic synapses onto dopamine neurons, with a particular interest in NMDA glutamate receptors: how they are regulated, and the balance between physiological and pathological NMDA receptor activity. Learn more Research Areas Neurobiology

Whole-cell patch clamp recording. Recordings were made at 28°C using the whole-cell patch-clamp technique from 1.5 to 8 h after ... On-line data collection and analysis were performed using an EPC-7 patch-clamp amplifier (HEKA Electronik), a Mac mini-computer ... Voltage-clamp recordings of eEPSCs in SCN neurons of (A) WT; (B) control (Opn4+/+; Vglut2loxP/loxP); (C) cKO (Opn4Cre/+; Vglut2 ... EPSCs (membrane potential clamped at -60 mV) were evoked by stimulation of the optic chiasm in the absence of CNQX (left ...

... with the use of the whole cell version of the patch-clamp technique. In CA1 cells IK was blocked by TEA at +30 mV with an IC50 ... A-current is reduced in the presence of amyloid-beta (Chen C, 200514 ). Patch-clamp recordings reveal a high density of A-type ... A-current is reduced in the presence of amyloid-beta (Chen C, 200514 ). Patch-clamp recordings reveal a high density of A-type ... A-current is reduced in the presence of amyloid-beta (Chen C, 200514 ). Patch-clamp recordings reveal a high density of A-type ...

7:547-556). Channels were expressed in oocytes and currents were measured with the cut-open-oocyte and patch-clamp techniques. ...