Selected oocytes were divided into groups for IVM over 48 h for each of the diestrus and anestrus stages as follows: Group Ia, ... Afterwards, oocytes were fixed with acetic acid-ethyl alcohol and stained with aceto-orcein to determine nuclear maturation. ... In conclusion, in the oocytes obtained from bitches in diestrus and anestrus supplemented with FCS or BSA in SOF medium without ... and oocytes were harvested by slicing. The oviducts were flushed with TCM-199 containing 10% FCS and were scraped and squeezed ...

Single-cell analysis of the developing mouse oocytes showed the preferential increase in mutant over wild-type mtDNA in the ... In m.5024C>T mice, this can be explained by the preferential propagation of mtDNA during oocyte maturation, counterbalanced by ... Single-cell analysis of the developing mouse oocytes showed the preferential increase in mutant over wild-type mtDNA in the ... In m.5024C,T mice, this can be explained by the preferential propagation of mtDNA during oocyte maturation, counterbalanced by ...

The graph has values for the oocyte in different stages. Oocytes without a zona pellucida and up to one layer of flattened ... Primary follicles were classified as oocytes surrounded by one layer of cuboidal granulosa cells. Oocytes with two or more ... Diameter of oocyte in graaffian stage. Value. 120 µm Range: Graph - link µm ... Comparative analysis of follicle morphology and oocyte diameter in four mammalian species (mouse, hamster, pig, and human). J ...

... stage 11 and stage 12 oocyte (Rs = 0.94), stage 12 and stage 13 oocyte (Rs = 0.88), as well as png and wild-type oocytes at ... The TE data for stage 14 oocytes were from Kronja et al. (2014b). Values from stage 14 oocytes are compared to those of stage ... TE values (log2) for stage 11, 12, 13, and 14 oocytes, or for stage 14 oocytes, 0-1 hr embryos, and 2-3 hr embryos were ... Thus, comparison of oocytes from stages 11 through 14 captures the events of meiotic progression and oocyte differentiation ...

We obtained cumulus-oocyte complexes containing metaphase II (MII) oocytes from the ovaries of oocyte-specific Uhrf1 KO females ... MII oocytes were obtained as cumulus-oocyte complexes from oviducts of ≥ 8-wk-old females injected with 7.5 U of pregnant mare ... Role of cytoplasmic UHRF1 in oocytes. Shuhei Uemura, Shoji Maenohara, Kimiko Inoue, Narumi Ogonuki, Shogo Matoba, Atsuo Ogura, ... Role of cytoplasmic UHRF1 in oocytes. Shuhei Uemura, Shoji Maenohara, Kimiko Inoue, Narumi Ogonuki, Shogo Matoba, Atsuo Ogura, ...

In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F- ... In Dystroglycan germline clones early oocyte polarity markers fail to be localized to the posterior, and oocyte cortical F- ... Dystroglycan is required for polarizing the epithelial cells and the oocyte in Drosophila.. *Mark ... and the oocyte (anteroposterior polarity). Loss of... (More). The transmembrane protein Dystroglycan is a central element of ...

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First the oocytes inserted in one drop of PBI solution by using an oral pipette. Then the oocytes transferred to another dish ... The rehydrated oocytes were washed in PBI solution. Finally the washed oocytes were transferred to T6 medium +5 mg mL-1 BSA. ... Then naked oocytes were evaluated by the inverted microscope. Oocyte metaphase II stages with first polar bodies were separated ... This cause the period of the oocyte shorter is exposed to the freezing solution that in this case is harmful to the oocyte ( ...

Abdominal injection of Vivo-Morpholino in fish for oocyte knockdown. Submitted by Jon-D on Wed, 03/01/2017 - 04:04 ... Injection of Vivo-Morpholinos into the abdominal cavity of medaka was used to knock down mPRα in the oocytes. ... of the medaka and role in the induction of oocyte maturation. Biomed Res. 2017;38(1):79-87. doi: 10.2220/biomedres.38.79. ...

Alteration of mRNA abundance, oxidation products and antioxidant enzyme activities during oocyte ageing in common carp Cyprinus ... Alteration of mRNA abundance, oxidation products and antioxidant enzyme activities during oocyte ageing in common carp Cyprinus ... Alteration of mRNA abundance, oxidation products and antioxidant enzyme activities during oocyte ageing in common carp Cyprinus ...

Effects of steroids on gamma-aminobutyric acid receptors expressed in Xenopus oocytes by poly(A)+ RNA from mammalian brain and ... Effects of steroids on gamma-aminobutyric acid receptors expressed in Xenopus oocytes by poly(A)+ RNA from mammalian brain and ... Effects of steroids on gamma-aminobutyric acid receptors expressed in Xenopus oocytes by poly(A)+ RNA from mammalian brain and ... Effects of steroids on gamma-aminobutyric acid receptors expressed in Xenopus oocytes by poly(A)+ RNA from mammalian brain and ...

Functional Coupling of Human L-Type Ca2+Channels and Angiotensin AT1A Receptors Coexpressed inXenopus laevis Oocytes: ... Functional Coupling of Human L-Type Ca2+Channels and Angiotensin AT1A Receptors Coexpressed inXenopus laevis Oocytes: ... Functional Coupling of Human L-Type Ca2+Channels and Angiotensin AT1A Receptors Coexpressed inXenopus laevis Oocytes: ... Functional Coupling of Human L-Type Ca2+Channels and Angiotensin AT1A Receptors Coexpressed inXenopus laevis Oocytes: ...

... spindles of vertebrate oocytes must remain stable and correctly organized during the arrest in metaphase II of meiosis. Using ... Timing of Nuclear Maturation and Postovulatory Aging in Oocytes of In Vitro-Grown Mouse Follicles with or Without Oil Overlay1 ... Transmission of Y chromosomes from XY female mice was made possible by the replacement of cytoplasm during oocyte maturation ... DOC1R: a MAP kinase substrate that control microtubule organization of metaphase II mouse oocytes. Development (2003) 130 (21 ...

Tewarson N.C., Immunocytochemical localization of (Apis mellifera) protein in growing oocytes of a haemophagous mite (Varroa ... Dittmann F., Steiner J., Intercellular connection between the lyrate organ and the growing oocyte in Varroa jacobsoni as ...

Mapping of epitopes on porcine zona pellucida-3 alpha by monoclonal antibodies inhibiting oocyte-sperm interaction.. Title. ... Mapping of epitopes on porcine zona pellucida-3 alpha by monoclonal antibodies inhibiting oocyte-sperm interaction.. ... Mapping of epitopes on porcine zona pellucida-3 alpha by monoclonal antibodies inhibiting oocyte-sperm interaction. ...

... expressed in Xenopus oocytes assessed as effect on channel currents at -80 mV holding potential at 100 uM by two-electrode ...

Read chapter Appendix B Public Workshop on Assessing the Medical Risks of Human Oocyte Donation for Stem Cell Research: It is ... The oocyte donation process is not without risk, however. Donors are given doses of hormones to trigger the production of more ... Appendix B Public Workshop on Assessing the Medical Risks of Human Oocyte Donation for Stem Cell Research 67-72 ... Assessing the Medical Risks of Human Oocyte Donation for Stem Cell Research: Workshop Report (2007) Chapter: Appendix B Public ...

Silbers chapter on transplantation of ovarian tissue or immature oocytes. ... Spindle transfer from the patients metaphase II oocyte into the cytoplasm of a donor oocyte. (A) The patients oocyte ... who underwent IVF after reconstitution of her oocytes by spindle transfer into the cytoplasm of enucleated donor oocytes. A ... Live Birth Derived From Oocyte Spindle Transfer To Prevent Mitochondrial Disease. By: John Zhang, Hui Liu, Shiyu Luo, Zhuo Lu, ...

In summary, we developed an approach to extract total RNA from single oocytes. The sequencing of single oocytes and their ... The oocyte acquires developmental competence as it progresses through folliculogenesis. It does so, by communicating with the ... Analyses of single oocytes are essential for further clarification of these molecular mechanisms. Standard protocols to obtain ... We profiled the total RNA of single oocytes (bovine and porcine), and observed distinct peaks for small RNAs, 18S, and 28S. We ...

Effects of Yu Linzhu on ovarian function and oocyte mitochondria in natural aging mice ...

Oocyte Cryopreservation for Donor Oocyte In Vitro Fertilization and Planned Oocyte Cryopreservation. ... Oocyte Cryopreservation for Donor Oocyte In Vitro Fertilization and Planned Oocyte Cryopreservation ... Evidence-based outcomes after oocyte cryopreservation for donor oocyte in vitro fertilization and planned oocyte ... Recipients can be counseled that the length of time that oocytes have been stored is not associated with differences in oocyte ...

... have different specific activities in Xenopus oocytes. ...

scientific article published on 01 January 1979

The analyses were carried out in unfertilized oocytes, newly fertilized oocytes and embryos at the stages of mid-blastula ... Transcripts found in unfertilized oocytes also encoded a large number of proteins implicated in cell adherence, tight junction ... Numerous genes transcribed in oocytes are involved in multiple aspects of cell maintenance and protection, including metabolism ... The analyses were carried out in unfertilized oocytes, newly fertilized oocytes and embryos at the stages of mid-blastula ...

Short-term resveratrol treatment restored the quality of oocytes in aging mice ...

All oocytes were placed in the EmbryoScope + incubator post-sperm injection with all annotations performed retrospectively by ... one embryologist (L-SO). Timing parameters included 2nd polar body extrusion (tPB2), sperm-originated PN (tSPNa) or oocyte- ... All oocytes were placed in the EmbryoScope + incubator post-sperm injection with all annotations performed retrospectively by ... Morphometric and morphokinetic differences in the sperm- and oocyte-originated pronuclei of male and female human zygotes: A ...

Figure 3. Transformation of Oocytes during Vitrification.. Panel A shows mature fresh oocytes. Panel B shows vitrified oocytes ... Among these options, ovarian stimulation and oocyte retrieval are more reliable than the aspiration of immature oocytes for in ... 52 or a 3 percent pregnancy rate per thawed oocyte.53 Fewer than 100 births have been reported to date from oocyte ... aspiration of immature oocytes from fresh tissue or follicular aspirates may be attempted so that oocytes can then undergo in ...

The recruitment of viltellogenic oocytes was 3013 oocytes per day on 3 August and declined rapidly as spawning approached. In ... The growth rate of the leading oocyte cohort was 2.73 × 10−5 mm3 per day on 20 September and increased to 3.91 × 10−3 at a mean ... Determinacy of fecundity and oocyte atresia in sole (Solea solea) from the Channel, the North Sea and the Irish Sea. ... In ICES areas IV and VII the prevalence of atretic oocytes in pre-spawning fish varied between 0.04 and 0.69 and the relative ...

First, sperm and oocyte plasma membrane proteins with affinity for proteins of the plasma membrane of their partner gamete were ... Approximately 100 oocyte plasma membrane proteins with affinity for the sperm, and 100 sperm plasma membrane proteins with ... The IZUMO1 protein and its receptor is the only sperm/oocyte interaction pair known to date that is necessary for gamete fusion ... The current study aims to find potential candidate proteins for sperm/oocyte plasma membrane interaction in a porcine model. ...

Oocytes. Rana piptens from New Jersey were kept in spring water at 4 °C. Only mature oocytes from healthy females were used. ... Our results show the model proposed for Eurycea oocytes is also applicable to those of Rana and probably to amphibian oocytes ... kf for immature Bufo oocytes, at 17 ‐23 °C, is 2·7 × 10−6 s−1 (Dick & Lea, 1964). The microvillous surface of oocytes makes ... The ratio kf / ks is 56; that of the surface-to-volume ratio of vesicle to oocyte is not less than 7·5 × 103, and greater, by a ...