Aberrant Epidermal Growth Factor Receptor Signaling and Enhanced Sensitivity to EGFR Inhibitors in Lung Cancer | Cancer Research
We next examined the expression of ErbB family members in these eight NSCLC cell lines ( Fig. 4A ). EGFR protein levels were ... our results are consistent with an up-regulated proliferation pathway involving the Ras oncogene and mitogen-activated protein ... Protein concentrations were estimated with a Protein Assay kit (Bio-Rad, Hercules, CA), and equal amounts of protein were ... Protein was quantified with a DC Protein Assay kit (Bio-Rad) in which 150 μg of lysate, boiled in the presence of SDS-PAGE ...
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Bostwick D. G. c-erbB-2 oncogene expression in prostatic intraepithelial neoplasia: mounting evidence for a precursor role. J. ... erbB-2 gene amplification and protein expression in human breast carcinoma with nodal status and nuclear grading. Cancer Res., ... Myers R. B., Srivastava S., Oelschlager D. K., Grizzle W. E. Expression of p160erbB-3 and p185erbB-2 in prostatic ... myc proto-oncogenes in breast carcinomas correlate with poor short-term prognosis. Oncogene, 1: 423-430, 1987. ...
CD151 Accelerates Breast Cancer by Regulating α6 Integrin Function, Signaling, and Molecular Organization | Cancer Research
CD151 (SFA-1, PETA-3), one of 33 proteins in the mammalian tetraspanin protein family ( 1), is widely expressed on the surface ... α6β4 and α6β1 integrins associate with ErbB-2 in human carcinoma cell lines. Exp Cell Res 1997; 236: 76-85. ... Torres-Arzayus MI, Yuan J, DellaGatta JL, Lane H, Kung AL, Brown M. Targeting the AIB1 oncogene through mammalian target of ... CD151 affects integrin associations with other proteins. CD151 may link laminin-binding integrins to other proteins within the ...
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Total protein was measured using the BCA protein detection method (Pierce). Cell lysates were aliquoted and stored at −80°C. ... Slides were hybridized with either HER2 (c-erbB-2 oncoprotein, 1:8,000 dilution for 1 hour, DAKO), β-catenin (clone 14, 1:500 ... β-catenin is considered an oncogene and its dysregulation or mutational activation can lead to cancer ( 2). Unlike colorectal ... 3A and a low β-catenin protein-expressing tumor in Fig. 3B. We found very few cases (,10) with nuclear staining; thus, for the ...
Inhibition of Fatty Acid Synthase Induces Endoplasmic Reticulum Stress in Tumor Cells | Cancer Research
... erbB-2) oncogene. Ann Oncol 2005; 16: 1253-67. ... Protein aliquots were then resolved by SDS-PAGE and new protein ... Induction of CCAAT/enhancer-binding protein (C/EBP)-homologous protein/growth arrest and DNA damage-inducible protein 153 ... Cox JS, Chapman RE, Walter P. The unfolded protein response coordinates the production of endoplasmic reticulum protein and ... Metabolic labeling of protein and fatty acid synthesis. To measure fatty acid synthesis, 1 × 105 cells per well were seeded in ...
Global Gene Expression Analysis of Gastric Cancer by Oligonucleotide Microarrays | Cancer Research
Overexpression of the heat shock protein HSP70 family and p53 protein and prognosis for patients with gastric cancer. Oncology ... for cholesterol transport to mitochondria from the c-ERBB-2 amplicon by a modified cDNA selection method. Cancer Res., 57: 3548 ... Oncogene, 19: 4947-4953, 2000.. OpenUrlCrossRefPubMed. *↵ Arias A. M. Epithelial mesenchymal interactions in cancer and ... Ulvsback M., Lindstrom C., Weiber H., Abrahamsson P. A., Lilja H., Lundwall A. Molecular cloning of a small prostate protein, ...
Length and Loss of Heterozygosity of an Intron 1 Polymorphic Sequence of egfr Is Related to Cytogenetic Alterations and...
Dimerization with other erbB receptors (erbB-2, -3, and -4) and the activation of the kinase domain are essential for ... Overexpression of EGFR protein has been linked to amplifications of the egfr gene located to 7p13-12. In the majority of the ... Translational research studies of erbB oncogenes: selection strategies for breast cancer treatment. Cancer Lett., 118: 143-151 ... 2B⇓ . When the 16-CA repeat allele was lost, there was a tentative lower intratumoral protein concentration than when the ...