Fluorescent in situ hybridization (FisH) Reagents. *Nucleic Acid EPP Loading Buffers. *Nucleic Acid Introduction Reagents* ... Carboxylic acids and derivatives*Carboxylic acid derivatives*Alpha-halocarboxylic acids and derivatives*Alpha-halocarboxylic ... Organic sulfonic acids and derivatives*Organosulfonic acids and derivatives*Alkanesulfonic acids and derivatives*Alkanesulfonic ... Naphthalene sulfonic acids and derivatives*1-naphthalene sulfonic acids and derivatives. *2-naphthalene sulfonic acids and ...

nucleic acids and oligonucleotides. *PNAs. Products & Ordering. EDA-IDP NU-837. EDA-ITP NU-836 ... fluorescence in situ hybridization (FISH). *quantitative real-time PCR → Molecular Biology. *signal transduction studies ...

Construction of chemically modified nucleic acids for application in electrochemical DNA sensors - graduate 2018 ... Study of electrochemical properties of of synthetic oligonucleotides and DNA modified with redox-active moieties - graduate ... Recent approaches in electrochemical analysis of damage, hybridization and interactions of DNA - graduate 2009 ...

Nucleic Acids Res. 10.1093/nar/gks808 Full Text. Check specificity/coverage. Use these options to reveal the in silico ... the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.. ... Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments. Coverage. ... Hybridization efficiency. Use this tool to assess in silico sensitivity (i.e. ...

Nucleic Acids Res. 10.1093/nar/gks808 Full Text. Check specificity/coverage. Use these options to reveal the in silico ... the hybridization efficiency of the oligonucleotide with its fully complementary target sequence, calculated with ProbeMelt.. ... Formamide. Percent formamide in the hybridization buffer for optimal hybridization conditions in FISH experiments. Coverage. ... Hybridization efficiency. References. Potential of a 16S rRNA-based taxonomic microarray for analyzing the rhizosphere effects ...

Base-pairing interactions between nucleic acids mediate target recognition in many biological. * Post author By ... Oligonucleotide probes (Table S2) labeled with photoswitchable dyes Alexa 647 and Alexa 568 were used to detect SgrS IL17RA (9 ... We combined single-molecule fluorescence hybridization (smFISH) (8) with single-molecule localization-based superresolution ... Base-pairing interactions between nucleic acids mediate target recognition in many biological processes. regulates several ...

Nucleic Acid, Sequence Alignment, Synteny. Abstract: The study of breakpoints that occurred during primate evolution promises ... Seven out of 11 genes that mapped to the breakpoint regions have been previously analyzed using oligonucleotide-microarrays. ... In Situ Hybridization, Fluorescence, Male, Pan troglodytes, Polymerase Chain Reaction, Primates, Repetitive Sequences, ... from its chimpanzee counterpart were identified by fluorescence in situ hybridization (FISH) and comparative sequence analysis ...

1985), Nucleic AcidHybridization, DL Press; Dracopoli et al. Current Protocols in HumanGenetics, John Wiley & Sons, Inc.; and ... These oligonucleotides can bearranged as cassettes. The cassettes can be joined up to form largergene assemblies, including a ... Another example of high stringent conditionsis hybridization in 7% SDS, 0.5 M NaPO4, pH 7, 1 mM EDTA at50 C, e.g., overnight, ... Also included are RNA and DNA-like or RNA-like materials,such as branched DNAs, peptide nucleic acids (PNA) or locked ...

End labeling of nucleic acids, Sequencing of nucleic acids, Nucleic acid hybridization, Making of labelled hybridization probes ... What are synthetic oligonucleotides ?. Synthetic oligonucleotides are single-stranded DNA fragments assembled from ... The five important ribonucleotides are adenylic acid, guanylic acid, uridylic acid, cytidylic acid and thymidylic acid. ... fate and function of amino acids, polypeptides, proteins, nucleic acids and their components (DNA & RNA), fatty acids (lipids ...

... requires the development of sensitive and fast devices to measure the presence of nucleic acid sequences by DNA hybridization. ... The resulting steroid oligonucleotide conjugates retain the hybridization and specific binding properties of oligonucleotides ... oligonucleotide conjugates has been performed by the reaction of oligonucleotides carrying amino groups with carboxyl acid ... by hybridization with the complementary oligonucleotide strands possessing SH groups previously immobilized. The capture of the ...

Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 2002;30:207-10. doi: ... Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics. 2003;4:249-64 ...

An overview of Fluorescent Oligonucleotide: Sensitive Fluorescent Oligonucleotide, ... Photophysical properties of fluorescent imaging biological probes of nucleic acids: SAC‐CI and TD‐DFT Study. ... fluorescent oligonucleotide probe 10.1002/cbic.201900555 In this work, a strategy for telomerase sensing by using hybridization ... Keywords related to Oligonucleotide. Asymmetrical Oligonucleotide Heteroduplex Oligonucleotide Decoy Oligonucleotide Antisense ...

Nucleic Acids Res. 2008;36(13):e80.. Article CAS PubMed PubMed Central Google Scholar ... Array comparative genomic hybridization and its applications in cancer. Nat Genet. 2005;37(Suppl):S11-17. ... Random DNA fragmentation allows detection of single-copy, single-exon alterations of copy number by oligonucleotide array CGH ... Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science. 1992;258(5083):818-21. ...

Nucleic acids were extracted from frozen tumor tissue (pre-NAT samples) using AllPrep DNA/RNA mini kit (Qiagen France SAS, ... Hybridization protocol. The extracted material was sent to Helixio (Saint-Beauzire, France), where it was hybridized with gene ...

... a synthetic peptide nucleic acid PNA-(CCCTAA)3 oligonucleotide probe16 for detecting consensus telomeric TTAGGG repeats; a β ... Assignment of human satellite 1 DNA as revealed by fluorescent in situ hybridization with oligonucleotides. Hum Genet1994;93: ... Chromosomal localization of human satellites 2 and 3 by a FISH method using oligonucleotides as probes. Hum Genet1994;93:383-8. ... Chromosomal location by in situ hybridization of the human Sau3A family of DNA repeats. Hum Genet1987;75:326-32. ...

... target hybridization mechanism that is in agreement with observed hybridization and washing properties of short oligonucleotide ... Explaining differences in saturation levels for Affymetrix GeneChip (R) arrays NUCLEIC ACIDS RESEARCH Skvortsov, D., Abdueva, D ... We explore experimentally the effect of the stringent wash, target concentration and hybridization time on the final microarray ... The experimental spike-in studies of microarray hybridization conducted by Affymetrix demonstrate a nonlinear response of ...

N2-Pyrene-functionalized 2-amino-?-L-LNAs (locked nucleic acids) display extraordinary affinity toward complementary DNA ... targets due to favorable preorganization of the pyrene moieties for hybridization-induced intercalation. Unfortunately, the ... Oligonucleotides Identity. Digital Object Identifier (DOI) * 10.1021/jo201095p PubMed ID * 21827174 Additional Document Info. ...

Zip nucleic acids (ZNAs) are oligonucleotides conjugated with a cationic spermine tail that increases the affinity of the ... In Situ Hybridization (ISH). A very low Tm for oligonucleotides or very short probes is a problem that can be circumvented by ... The ability to modulate the global charge of conjugated ZNAs based on the number of spermines connected to the nucleic acid ... The Tm increases linearly with the length of the oligonucleotide. They are a good alternative for Minor Groove Binder (MGB) and ...

"Development and Application of Nucleic Acid Probes in Bacterial Systematics, " in Sequencing and Hybridization Techniques in ... The DNA fragment from the PCR reaction would be probed to a gene array bearing 100,000 small oligonucleotides representing the ... "Development and Application of Nucleic Acid Probes in Bacterial Systematics, " in Sequencing and Hybridization Techniques in ... "Development and Application of Nucleic Acid Probes in Bacterial Systematics, " in Sequencing and Hybridization Techniques in ...

The use of biosensors in nucleic acid detection technologies is on the rise. For example, Aptitude Medical uses electrochemical ... and hybridization of target DNA oligomers with probe DNA oligonucleotides modulates the current. Because single-stranded and ... The nanomechanoelectrical approach can now potentially be used for different nucleic acid detection needs. For example, "the ... "With different nanomaterials and electrical biosensing techniques, we are now capable of producing nucleic acid sensors that ...

Nucleic Acids Research 1992, 20, 4711-4716.. How many PhD students and postdocs do you currently supervise? Are you currently ... analogues that were functionalized for automated oligonucleotide synthesis and studied their properties such as hybridization ... His purpose was to find why nature had chosen for pentose and not hexose nucleic acids. Finally we published almost together in ... the group of Albert Eschenmoser at the ETH in Zurich also started investigating the synthesis of hexose nucleic acids. ...

During the proposed project new, nucleic acid-based analytical techniques will be used to gain insight into specific effects of ... To establish a selective "hgh-chip" the newly designed oligonucleotide probes for the hghcandidate genes will be printed on a ... To find additional hgh-candidate genes, suppression subtractive hybridization (SSH) technology will be applied on hgh-treated ... After confirming SSH-genes to be differentially expressed in a series of microarray experiments, oligonucleotide probes will be ...

A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells ... Comparative Genomic Hybridization. *Heteroduplex Analysis. *In Situ Hybridization. *Oligonucleotide Array Sequence Analysis ... "In Situ Hybridization" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... This graph shows the total number of publications written about "In Situ Hybridization" by people in UAMS Profiles by year, and ...

A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells ... In Situ Hybridization. *Oligonucleotide Array Sequence Analysis. *Subtractive Hybridization Techniques. *Staining and Labeling ... "In Situ Hybridization" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... This graph shows the total number of publications written about "In Situ Hybridization" by people in this website by year, and ...

PPT enhancement was recorded to significantly improve the hybridization kinetics and thus specific detection of nucleic acid, ... Using AuNPs capped with thiol-modified antisense oligonucleotides (ASOs) [AuNPs-ASOs], a colorimetric biosensing approach has ... a) Schematic illustration of the hybridization of two complementary strands. (b) Real-time hybridization of RdRp-COVID and its ... d) Schematic illustration of inhibited hybridization of two partially matched sequences. The reddish arrows indicated the ...

Nucleic Acids Res. 2006, 34 (Database issue): D108-110. 10.1093/nar/gkj143. ... Exon array hybridizations. For measuring the expression values of the hiPSC samples, the RNA was isolated using RNeasy Kit ( ... Bolstad BM, Irizarry R, Astrand M, Speed T: A comparison of normalization methods for high density oligonucleotide array data ... Kapur K, Jiang H, Xing Y, Wong WH: Cross-hybridization modeling on Affymetrix exon arrays. Bioinforma Oxf Engl. 2008, 24: 2887- ...

In this review, we focus on the use of antisense oligonucleotides (antimiRs) in miRNA inhibition for loss-of-function studies. ... We provide an overview of the currently employed antisense chemistries and their utility in designing antimiR oligonucleotides ... Efficient inhibition of miR-155 function in vivo by peptide nucleic acids. Nucleic Acids Res. 2010, 38: 4466-4475. 10.1093/nar/ ... However, this only holds true if the hybridization stringency can be controlled. When antimiRs are used in vivo, their ...

optimum hybridisation had been the 11mers to 15mers. For oligonucleotides longer than 15 nt the strength from the hybridisation ... Nucleic Acids Res., 25, 3059C3065. [PMC free article] [PubMed] 23. Seela F., Tran-Thi,Q.-H. and Franzen,D. (1982) Nucleic Acids ... Some regions of nucleic acid targets arent accessible to heteroduplex formation with complementary oligonucleotide probes ... Some regions of nucleic acid targets arent accessible to heteroduplex formation. * Post author By conferencedequebec ...

... of nucleic acids 82 Restriction enzyme digests 82 Sequencing and database analysis 83 Whole mount in situ hybridisation 83 In ... The knockdown of the crypic homolog in Xenopus, Xcr2, by morpholino antisense oligonucleotides embryos also had the same effect ... into cloning vectors 79 Transformation and clonal selection 80 Table of contents Preparation and handling of nucleic acids 81 ... vitro transcription of RNA probes 83 In situ hybridisation 83 Histological analysis of embryos after in situ hybridisation 85 ...