No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution. ... selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. ... A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or ... The state-of-the-art nucleic acid hybridization assay techniques generally involve immobilization of the sample nucleic acid on ...
Our goal was to develop and optimize a novel Peptide Nucleic Acid (PNA) Fluorescence in situ Hybridization assay (PNA FISH) for ... From: Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and ... Moderate hybridization; (+++) Good hybridization; (++++) Optimal hybridization. The table shows the median value from the three ... The PNA probe (Lac663 and Gar162) efficiencies were tested in each sample with the following hybridization PNA FISH qualitative ...
Hybridization with nucleic acid probes, P. Tijssen Label Hybridization with nucleic acid probes, P. Tijssen. Publication * ... Hybridization with nucleic acid probes. Title Hybridization with nucleic acid probes. Statement of responsibility P. Tijssen. ... link.lib.rpi.edu/portal/Hybridization-with-nucleic-acid-probes-P./EexS9d6Bhuk/,Hybridization with nucleic acid probes, P. ... link.lib.rpi.edu/portal/Hybridization-with-nucleic-acid-probes-P./EexS9d6Bhuk/,Hybridization with nucleic acid probes, P. ...
1984); Nucleic Acid Hybridization (B.D. Hames & SJ. Higgins eds. (1985)); Transcription And Translation (B.D. Hames & SJ. ... A nucleic acid molecule is "hybridizable" to another nucleic acid molecule, such as a cDNA, genomic DNA, or RNA, when a single ... stringency for hybridizing nucleic acids depends on the length of the nucleic acids and the degree of complementation, ... "nucleic acid sequence" or "nucleotide sequence" is a series of nucleotide bases (also called "nucleotides") in a nucleic acid, ...
Using LNA probes, the hybridization step was shortened to 1 h. After the hybridization step, 84.6% RNA was preserved; thus, we ... MRNA quantification after fluorescence activated cell sorting using locked nucleic acid probes. / Maruo, Rie; Yamada, Hiroya; ... In this study, we used locked nucleic acid (LNA) oligonucleotides, which are known to enhance both the sensitivity and ... In this study, we used locked nucleic acid (LNA) oligonucleotides, which are known to enhance both the sensitivity and ...
Nucleic Acids Res 28(12):e63View ArticleGoogle Scholar. *. Qiao G, Lee DC, Woo SH, Li H, Xu DH, Park SI (2012) Microbiological ... 2014), colony hybridization by species-specific probes to identify V. scophthalmi in the intestinal microbiota of fish and an ... 2000) as a novel nucleic acid detection, is a desirable diagnostic tool for on-site epidemiological investigations of bacterial ... Multi-probe-fluorescence in situ hybridization for the rapid enumeration of viable Vibrio parahaemolyticus. Microbes Environ 24 ...
Nucleic Acids Res. 2003 Jul 1;31(13):3406-15. Research Support, U.S. Govt, P.H.S. ... Nucleic Acids Res. 2003 Jul 1;31(13):3406-15.. Mfold web server for nucleic acid folding and hybridization prediction.. Zuker M ... for the prediction of the secondary structure of single stranded nucleic acids. The objective of this web server is to provide ... easy access to RNA and DNA folding and hybridization software to the scientific community at large. By making use of ...
... and applications of the nucleic acid fragments in the diagnosis of mycobacterial infections are described. More particularly, ... Nucleic acid fragments have been obtained from the genome of mycobacteria, ... Nucleic acid fragments have been obtained from the genome of mycobacteria, and applications of the nucleic acid fragments in ... Nucleic acid fragments have been obtained from the genome of mycobacteria, and applications of the nucleic acid fragments in ...
... for specific nucleic acid sequences can be immobilized as arrays on solid phase surfaces for detection of multiple nucleic acid ... particularly those used for plasmon surface detection and electron transfer detection of nucleic acid. The probes can be washed ... structure between the fluorophore label and a point of attachment to a solid phase is useful as a probe to detect nucleic acid ... A fluorescently labeled nucleic acid having a hairpin ... Immobilized nucleic acid hybridization reagent and method. US ...
In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ... "Nucleic Acid Hybirdizations". DNA - Basics of Structure and Analysis. Retrieved 26 May 2017. Beckman, Mary. "Hybirdization". ... Felsenfeld, G; Miles, HT (1967). "The physical and chemical properties of nucleic acids". Annual Review of Biochemistry. 36: ... Fluorescence in situ hybridization (FISH) is a laboratory method used to detect and locate a DNA sequence, often on a ...
Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization, and NGS approaches.. Khodakov D1, Wang C1, ... Nucleic acid analysis technologies being translated into the clinic can broadly be classified into hybridization, PCR, and ... Nucleic acid sequence variations have been implicated in many diseases, and reliable detection and quantitation of DNA/RNA ...
The methodology is able to detect even a single-copy of a specific nucleic acid of interest under controlled conditions ... or whether the nucleic acid sequence of interest is localized in the chromosomes, nucleus, or cytoplasm of a cell. The methods ... such that a relatively unskilled person can accurately and reproducibly detect even a single-copy of a specific nucleic acid of ... Improved methodologies for in-situ hybridization and non-isotopic detection of nucleic acid sequences are provided which offer ...
Buy the Paperback Book Nucleic Acids Hybridization by Anton Buzdin at Indigo.ca, Canadas largest bookstore. + Get Free ... Nucleic Acids Hybridization: Modern Applications. EditorAnton Buzdin, Sergey Lukyanov. Paperback , October 19, 2010. ... Title:Nucleic Acids Hybridization: Modern ApplicationsFormat:PaperbackDimensions:336 pagesPublished:October 19, 2010Publisher: ... in solution for mutation detection Anton BuzdinCurrent attempts to improve the specificity of nucleic acids hybridization Anton ...
Nucleic acid hybridization has been used in an attempt to determine the fraction of spleen DNA which is homologous to ... Quantitation of Immunoglobulin Genes by Nucleic Acid Hybridization with RNA from Myeloma and Spleen Microsomes. Ursula Storb ... Quantitation of Immunoglobulin Genes by Nucleic Acid Hybridization with RNA from Myeloma and Spleen Microsomes ... Quantitation of Immunoglobulin Genes by Nucleic Acid Hybridization with RNA from Myeloma and Spleen Microsomes ...
Nucleic Acid Hybridization Nucleic acids Complementary bases Hybridization Complementary strands from any sources Reversible ... Nucleic Acid Hybridization. Description:. Nucleic Acid Hybridization Nucleic acids Complementary bases Hybridization ... Nucleic Acid Hybridization - Nucleic Acid Hybridization. Nucleic acid hybridization is a fundamental tool in ... Benton-Davis. ... Nucleic Acid Hybridization. 1. Nucleic Acid Hybridization. Nucleic acids Complementary bases Hybridization Complementary ...
... in situ nucleic acid hybridization and an immunocytochemical [alkaline phosphatase-antialkaline phosphatase (APAAP)] technique ... whereas RNA-RNA hybridization is a sensitive technique which can be applied to material stored for years. ... Detection of Yellow Fever Viral RNA by Nucleic Acid Hybridization and Viral Antigen by Immunocytochemistry in Fixed Human Liver ... We therefore compared 2 virus-specific assays applicable to fixed liver, in situ nucleic acid hybridization and an ...
... ... "Diagnostics based on nucleic acid sequence variant profiling: PCR, hybridization, and NGS approaches." Advanced Drug Delivery ... Nucleic acid analysis technologies being translated into the clinic can broadly be classified into hybridization, PCR, and ... Nucleic acid sequence variations have been implicated in many diseases, and reliable detection and quantitation of DNA/RNA ...
Hybridization Biosensors Relying on Electrical Properties of Nucleic Acids. Research output: Contribution to journal/Conference ...
Nucleic Acid Hybridization: Principles and Applications (Human Molecular Genetics) as an eTextbook and get instant access. ... Chapter 07 - Nucleic Acid Hybridization: Principles and Applications (Human Molecular Genetics) by Tom Strachan, Andrew Read ... Chapter 07 - Nucleic Acid Hybridization: Principles and Applications (Human Molecular Genetics) 4th Edition by Tom Strachan, ...
The present invention relates to the detection of a target nucleic acid sequence using a target hybridization and detection ... The term used herein "target nucleic acid", "target nucleic acid sequence" or "target sequence" refers to a nucleic acid ... the target nucleic acid sequence is a pre-amplified nucleic acid sequence. The utilization of the pre-amplified nucleic acid ... hybridization of the primer with the target nucleic acid sequence and incubation with the template-dependent nucleic acid ...
plasmid RSF2124 nucleic acids; bacteriophage φX174 nucleic acids; Escherichia coli nucleic acids. ... Studies on Nucleic Acid Reassociation Kinetics: Retarded Rate of Hybridization of RNA with Excess DNA ... Studies on Nucleic Acid Reassociation Kinetics: Retarded Rate of Hybridization of RNA with Excess DNA. Proceedings of the ... We gratefully acknowledge the gifts of φX174 nucleic acids by Drs. Lloyd H. Smith, Amy S. Lee, Paul H. Johnson, and Robert L. ...
Arrays of nucleic acids are formed by techniques, preferably high resolution, light-directe ... Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. ... Devices and techniques for hybridization of nucleic acids and for determining the sequence of nucleic acids. Arrays of nucleic ... target nucleic acid is determined by hybridization to an array of nucleic acid probes on a substrate. Each probe is located at ...
Toward an on-chip multiplexed nucleic acid hybridization assay using immobilized quantum dot-oligonucleotide conjugates and ... Target nucleic acid sequences hybridized with QD-probe conjugates and were labeled with Cy3 or Alexa Fluor 647 as acceptor dyes ... A new strategy is presented for the development of multiplexed DNA hybridization assays using immobilized QDs in a microfluidic ...
Broder, Graham Richard (2011) The adaption of an encoded microparticle array for multiplexing nucleic acid hybridisation assays ... The adaption of an encoded microparticle array for multiplexing nucleic acid hybridisation assays ... The adaption of an encoded microparticle array for multiplexing nucleic acid hybridisation assays ... The adaption of an encoded microparticle array for multiplexing nucleic acid hybridisation assays ...
Implications for DNA hybridization. Einert, T R and Orland, H and Netz, R R - 2011. ... Secondary structure formation of homopolymeric single-stranded nucleic acids including force and loop entropy: Implications for ... Secondary structure formation of homopolymeric single-stranded nucleic acids including force and loop entropy: Implications for ... Secondary structure formation of homopolymeric single-stranded nucleic acids including force and loop entropy: ...