... amplification-free bait capture enriched sequencing of cDNA is a viable method for generating a SARS-CoV-2 genome sequence and ... All libraries were sequenced using an Illumina MiniSeq with data analyzed using a standardized bioinformatics workflow (SARS- ... Genome sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is increasingly important to monitor the ... Genome Sequencing. Total nucleic acid was extracted from each mid-turbinate swab using the QIAamp Viral RNA Mini kit (Qiagen, ...

HMMER web server: interactive sequence similarity searching. Nucleic Acids Research, 39(Web-Server-Issue):29-37, 2011. URL: ... An improved GPU-based SAT model counter. In Thomas Schiex and Simon de Givry, editors, Proceedings of the 25th International ... Counting-based reliability estimation for power-transmission grids. In Satinder P. Singh and Shaul Markovitch, editors, ... Knowledge-base degrees of inconsistency: Complexity and counting. In Proceedings of the AAAI Conference on Artificial ...

Nucleic acids research 40 (W1), W498-W504, 2012. 593. 2012. Functional microRNA targets in protein coding sequences. M Reczko, ... The DEF data base of sequence based protein fold class predictions.. M Reczko, H Bohr ... The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and ...

... has released an updated edition of Nucleic Acid Sequencing Methods in Diagnostic Laboratory Medicine; Approved Guideline... ... The Clinical and Laboratory Standards Institute (CLSI) has released an updated edition of Nucleic Acid Sequencing Methods in ... This document contains information necessary for clinical laboratories performing qualitative, multiplexed nucleic acid-based ... MM09-A2 addresses diagnostic sequencing using both automated capillary-based sequencers and massively parallel sequencing ...

The microarray technology is based on the hybridization of labelled DNA ... The principle of DNA microarray technology is based on the hybridization of complementary sequences of nucleic acid molecules. ... The microarray technology is based on the hybridization of labelled DNA or RNA molecules to complementary sequences that are ... The probes are complementary to specific sequences of DNA or RNA molecules that are to be analysed. The DNA or RNA molecules ...

Such tests use a nucleic acid hybridization technique that is based on double-stranded complementary pairing of nucleic acids. ... Basically, technicians synthesize a single-stranded nucleic acid sequence complementary to the DNA or RNA of a specific ... nucleic acid tests, antigen tests and antibody tests. The nucleic acid test is the gold standard for pathogenic diagnosis, ... Most of us know that there are various types of COVID test available, but the new technical specification uses nucleic acid ...

Nucleic Acid Conformation. *Molecular Sequence Data. *Magnetic Resonance Spectroscopy. *Biophysics. *Base Sequence ... a four base-pair helix II capped by a UUCG apical loop exhibits a higher degree of order compared to a six base-pair helix I ( ... Elongating helix I by three base-pairs equalizes the alignment of the two helices and pushes the RNA into the motional coupling ... Increasing the length of elongation further to 22 base-pairs pushes the RNA into the motional decoupling limit in which helix I ...

Nucleic Acids Res. 16 (21):9947.. Nyren et al. (1997) Detection of single-base changes using a bioluminometric primer extension ... Effect of template sequence and substrate variation on termination of synthesis. J. Biol. Chem. 263 (29):15094.. Nakamaye et al ... Molecular Formula: C10H16N5O11P3S (free acid) ... Molecular Weight: 507.24 g/mol (free acid). Exact Mass: 506.98 ... 1988) Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha ...

... nucleic acid types, sequence variation types, and types of sequencing. Variants queried shall be located in varying sequence ... panel shall include samples from at least two sources that have highly confident sequence based on well-validated sequencing ... measure and sort signals in order to analyze nucleic acid sequences in a clinical sample. The device may include a signal ... types of sequencing, and sequences located in varying sequence context (e.g., different chromosomes, GC-rich regions). The well ...

Implication for DNA sequencing by hybridization. Nucleic Acids Res. (1998), 26: 4249-4258.. 4. Kandimalla, E.R., Yu, D., Zhao, ... N4-Et-dC base pairs with dG, but the N4-Et-dC : dG base pair has a thermal stability similar to an A:T base pair instead of a C ... Nucleic Acids Res. (1997), 25: 3059-3065.. 3. Nguyen, H-K., Bonfils, E., Auffay, P., Costaglioli, P., et al. The stability of ... C base pairs, high-GC content sequences may contain mis-matches yet still stably hybridize to a probe or target (resulting in ...

Sequence-specific protein recognition of single-stranded nucleic acids is critical for many fundamental cellular processes, ... The thermodynamic and structural effects of these sequence perturbations could not have been predicted based solely upon the ... may be of general importance in protein recognition of single-stranded nucleic acids. This set of structural and thermodynamic ... Sequence Clusters. 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity. ...

... the articles that reported on virus detection were based on the detection of amplified viral SARS-CoV-2 nucleic acid sequences ... Different longitudinal patterns of nucleic acid and serology testing results based on disease severity of COVID-19 patients. ... WHO advice is that laboratory-based nucleic acid testing (such as RT-PCR) should be used to confirm cases with further ... confirmation by nucleic acid sequencing when necessary or feasible.66 The only suitable studies that allowed the diagnostic ...

... as well as against Araport11 for the Col-0 reference genome sequence. GeneSet_Nd-1_v1.1 contains previously missed non- ... The Arabidopsis thaliana Niederzenz-1 genome sequence was recently published with an ab initio gene prediction. In depth ... improve the annotation by transferring annotation information from the recently released Columbia-0 reference genome sequence ... Nucleic Acids Res. 1991;19(14):3795-8.. Article CAS PubMed PubMed Central Google Scholar ...

Loop-mediated isothermal amplification (LAMP), an isothermal nucleic acid amplification technique, has been extensively used in ... Up to now, the most effective and sensitive techniques used for GMO detection are based on polymerase chain reaction (PCR). PCR ... methods for GM crops and their products were developed to detect the presence of either protein or nucleic acid sequence of the ... As compared with protein-based methods, DNA-based methods, such as PCR or real-time quantitative PCR (RT-qPCR), are the most ...

Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303. ... Click molecule labels to explore molecular sequence information ... 4R5V: Structure Of The M1 Alanylaminopeptidase From Malaria Complexed With A Hydroxamic Acid-based Inhibitor. ...

Table 2: Example of applying N-grams on two nucleic acid sequences, the values between ... Sudha V.P., Vijaya M.S. (2018) Decision Tree Based Model for the Classification of Pathogenic Gene Sequences Causing ASD. In: ... Nucleic acids are the biopolymers that carry all genetic information of living organisms. The two main classes of nucleic acids ... Table 1: Example of extracting N-grams from a nucleic acid sequence ...

... such as nucleic acid hybridization probes, improved turnaround times significantly, but can lack specificity. Sequencing of ... Traditional methods for mycobacteria identification, primarily culture-based phenotypic methods, may take weeks and are often ...

... through base-pairing interactions with the target sequence, guide Cas nucleases to the invasive nucleic acid to cleave it. Upon ... Sequence-directed genetic interference pathways control gene expression and preserve genome integrity in all kingdoms of life. ... Within CRISPR sites, the repeats are separated by short spacer sequences that match phage or plasmid genomes and specify the ... Spacer sequences are transcribed into CRISPR RNAs (crRNAs)-small RNAs that, ...

IPR012340 Nucleic acid-binding, OB-fold, subgroup. *IPR005824 KOW. Prediction based on longest six frame method >SGN-P710409 ( ... mRNA member sequences (59) mRNA member sequences (59) Alignment image suppressed for unigene with 59 aligned sequences.. [Show ... Prediction based on ESTScan [ Show ESTScan Detail] >SGN-P670009 (159 Aa). ... Based on clone cLER-15-K10, annotated by Rob Alba Created 2003-01-20, last modified 2003-01-20 ...

Genes are assembled on the fly, based on the alignment of all reads against a protein reference database such as NCBI-nr. ... Specifically, the user selects a gene family based on a classification such as KEGG and all reads binned to that gene family ... Depending on the goals of the project, the short reads can either be subjected to direct sequence analysis or be assembled into ... Using published synthetic community metagenome sequencing reads and a set of 41 gene families, we show that the performance of ...

... single-use test cartridges perform automated nucleic acid extraction, PCR amplification, and detection of target sequences when ... The GeneXpert system is a market leader* in sample-to-answer PCR diagnostic testing with the largest global installed base and ... single-use test cartridges perform automated nucleic acid extraction, PCR amplification, and detection of target sequences when ... The cornerstone of the GeneXpert solution is Cepheids patented, single-use Xpert test cartridge, in which nucleic acid ...

Total nucleic acids were extracted automatically from 400 µL of samples, using a High Pure Viral Nucleic Acid Kit and iPrep™ ... with an ABI 7500 Fast Sequence Detection System. ... 100µL of extracted nucleic acids were stored at -70°C until ... Additional sentinel based surveillance embedded in influenza surveillance will be required to be established in order to fully ... We conducted a prospective study using sentinel-based influenza surveillance to detect RSV by real time PCR in patients with ...

... analysis of the resulting heteroduplexes facilitates the mapping of regions of base sequence homology of nucleic acids. ... "Nucleic Acid Heteroduplexes" by people in this website by year, and whether "Nucleic Acid Heteroduplexes" was a major or minor ... Double-stranded nucleic acid molecules (DNA-DNA or DNA-RNA) which contain regions of nucleotide mismatches (non-complementary ... "Nucleic Acid Heteroduplexes" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH ( ...

The first of these nucleic acids was made up of a repeating sequence of the two nucelotides U and C, which, after translation, ... were the first to determine the genetic code and show how the nucleic acid bases, with their alphabet made up of A, U, G, and C ... Robert holley and nucleic acid structure. Robert Holley from Cornell University discovered a particular type of nucleic acid, ... It was the first time the structure of a biologically active nucleic acid had been established. It emerged that tRNA was the ...

MicroarrayThe principle of DNA microarray technology is based on the hybridization of complementary sequences of nucleic acid ... The microarray technology is based on the hybridization of labelled DNA or RNA molecules to complementary sequences that are ... Introduction RNA, or Ribonucleic Acid, is a vital molecule in the process of protein synthesis. It serves as the template for ... RNA is synthesized from DNA, or Deoxyribonucleic Acid, and carries the genetic information from the DNA to the ribosome, where ...

Key: NASBA = nucleic acid sequence-based amplification; RT-PCR = reverse transcription polymerase chain reaction; TMA = ... Key: NASBA = nucleic acid sequence-based amplification; RT-PCR = reverse transcription polymerase chain reaction; TMA = ... Based on accumulated experience with currently available assays, the current definition of virologic suppression is a plasma ... Efavirenz-based antiretroviral therapy among nevirapine-exposed HIV-infected children in South Africa: a randomized clinical ...

Nucleic Acids Res. 43:D1049-D1056. 2015.PubMed/NCBI View Article : Google Scholar ... Ritchie ME, Phipson B, Wu D, Hu Y, Law CW, Shi W and Smyth GK: Limma powers differential expression analyses for RNA-sequencing ... Nucleic Acids Res. 51:D1257-D1262. 2023.PubMed/NCBI View Article : Google Scholar ... Nucleic Acids Res. 43(e47)2015.PubMed/NCBI View Article : Google Scholar ...

Nucleic acid sequencing can provide a detailed overview of microbial communities in comparison with standard plate-culture ... Evaluation of nanopore-based sequencing technology for gene marker based analysis of complex microbial communities. Method ... Calus, Szymon Tomasz (2018) Evaluation of nanopore-based sequencing technology for gene marker based analysis of complex ... ONT is an emerging company that developed the first-ever portable nanopore-based sequencing platform called MinIONTM. ...

6.7 million in new research grants to develop DNA sequencing technologies that are more sensitive, faster, cheaper and more ... in nucleic acid sequencing such as work focused on direct RNA sequencing to further improvements on nanopore-based sequencing ... In total, six new awards have been made through a program called the Novel Nucleic Acid Sequencing Technology Development ... Thanks to NHGRI, this program will continue to do wonders in order to improve nucleic acid sequencing technologies." ...

A web-based tool for the identification of origin of transfers in DNA sequences of bacterial mobile genetic elements. Nucleic ... amino acids, and bile acids metabolism and transport, the Cah autotransporter, fimbrial operons, a Fe3+/spermidine/putrescine ... Species and sequence types were assigned using Kleborate[14]. Whole-genome-based distance trees were inferred using Mashtree ... In addition, over ten compatibility groups were observed, predominating IncR, IncFIB(K), and IncFII(K). Upon sequence-based ...