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*  Isothermal Nucleic Acid Amplification Technology Market To Be Driven By Increasing Demand As A Molecular Testing Technique And...
The Global Isothermal Nucleic Acid Amplification Technology (INAAT) Market is expected to reach USD 4.3 billion by 2025, growing at a CAGR of 11.8%, according to a new report by Grand View Research, Inc. This can be attributed to significant rise in awareness regarding need for rapid and on-time testing for chronic and infectious diseases. Global increase in adoption of INAAT for detection of HIV, hepatitis A & B, and sexually transmitted diseases is considered to be a high impact rendering driver for growth.. Loop-Mediated Isothermal Amplification is one of the most preferred nucleic acid amplification methods because of its ability to provide point of care testing, thus leading to increased adoption. The recently-introduced Nicking Enzyme Amplification Reaction (NEAR) and Helicase-dependent amplification (HDA) technologies are expected to experience ...
http://www.bostonnewsdesk.com/story/144646/isothermal-nucleic-acid-amplification-technology-market-to-be-driven-by-increasing-demand-as-a-molecular-testing-technique-and-prevalence-of-infectious-diseases-till-2025-grand-view-research-inc.html
*  Isothermal Nucleic Acid Amplification Technology Market To Be Driven By Increasing Demand As A Molecular Testing Technique And...
The Global Isothermal Nucleic Acid Amplification Technology (INAAT) Market is expected to reach USD 4.3 billion by 2025, growing at a CAGR of 11.8%, according to a new report by Grand View Research, Inc. This can be attributed to significant rise in awareness regarding need for rapid and on-time testing for chronic and infectious diseases. Global increase in adoption of INAAT for detection of HIV, hepatitis A & B, and sexually transmitted diseases is considered to be a high impact rendering driver for growth.. Loop-Mediated Isothermal Amplification is one of the most preferred nucleic acid amplification methods because of its ability to provide point of care testing, thus leading to increased adoption. The recently-introduced Nicking Enzyme Amplification Reaction (NEAR) and Helicase-dependent amplification (HDA) technologies are expected to experience ...
http://www.gurgaon-samachar.com/story/30037/isothermal-nucleic-acid-amplification-technology-market-to-be-driven-by-increasing-demand-as-a-molecular-testing-technique-and-prevalence-of-infectious-diseases-till-2025-grand-view-research-inc.html
*  Isothermal Nucleic Acid Amplification Technology Market Is Projected To Grow Swiftly Due To Rising Demand As A Molecular...
The Global Isothermal Nucleic Acid Amplification Technology (INAAT) Market is expected to reach USD 4.3 billion by 2025, growing at a CAGR of 11.8%, according to a new report by Grand View Research, Inc. This can be attributed to significant rise in awareness regarding need for rapid and on-time testing for chronic and infectious diseases. Global increase in adoption of INAAT for detection of HIV, hepatitis A & B, and sexually transmitted diseases is considered to be a high impact rendering driver for growth.. Loop-Mediated Isothermal Amplification is one of the most preferred nucleic acid amplification methods because of its ability to provide point of care testing, thus leading to increased adoption. The recently-introduced Nicking Enzyme Amplification Reaction (NEAR) and Helicase-dependent amplification (HDA) technologies are expected to experience ...
http://www.wisconsinchronicle.com/story/148752/isothermal-nucleic-acid-amplification-technology-market-is-projected-to-grow-swiftly-due-to-rising-demand-as-a-molecular-testing-technique-till-2025-grand-view-research-inc.html
*  Rapid and Sensitive Detection of Salmonella spp. by Using a Loop-Mediated Isothermal Amplification Assay in Duck Carcass Sample...
Rapid and Sensitive Detection of Salmonella spp. by Using a Loop-Mediated Isothermal Amplification Assay in Duck Carcass Sample - loop-mediated isothermal amplification;Salmonella spp.;screening;duck;
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=CSSPBQ_2013_v33n5_655
*  A Loop-Mediated Isothermal Amplification Assay and Sample Preparation Procedure for Sensitive Detection of Xanthomonas...
A Loop-Mediated Isothermal Amplification Assay and Sample Preparation Procedure for Sensitive Detection of Xanthomonas fragariae in Strawberry. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
http://libros.duhnnae.com/2017/jun9/149852837778-A-Loop-Mediated-Isothermal-Amplification-Assay-and-Sample-Preparation-Procedure-for-Sensitive-Detection-of-Xanthomonas-fragariae-in-Strawberry.php
*  Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay, Archives of Virology | 10.1007...
Read "Detection of hepatitis C virus by an improved loop-mediated isothermal amplification assay, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
https://www.deepdyve.com/lp/springer_journal/detection-of-hepatitis-c-virus-by-an-improved-loop-mediated-isothermal-NXy50Kz6Q0
*  Application of loop-mediated isothermal amplification assay in the detection of herpesvirus of turkey (FC 126 strain) from...
This study was designed to optimize and apply the use of loop-mediated isothermal amplification (LAMP) as an alternative to conventional polymerase chain reaction (PCR) for the detection of herpesvirus of turkeys (HVT) (FC 126 strain) in vaccinated and non-vaccinated poultry in Nigeria
http://www.veterinaryworld.org/Vol.10/November-2017/17.html
*  Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method
BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject-specific sections.
https://www.hindawi.com/journals/bmri/2015/356401/abs/
*  Development of a Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of Avian and Animal Chlamydiosis | Center...
Chlamydiae are implicated in a variety of clinically and economically important diseases in livestock and companion animals. These bacteria are associated with abortion, conjunctivitis, encephalomyelitis, enteritis, pneumonia, and polyarthritis in ruminants. Infection with these bacteria is the most common cause of abortion in sheep and goats and also causes zoonotic infection in humans which, in pregnant women, can result in spontaneous abortion.
https://ag.umass.edu/cafe/nifa-planned-research-initiatives/development-of-loop-mediated-isothermal-amplification-method
*  PLOS Neglected Tropical Diseases: Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid Detection of Trypanosoma...
Author SummaryControl of human African trypanosomiasis (HAT) or sleeping sickness relies on diagnosis and treatment of infected patients. However, the diagnostic tests in routine use have limited sensitivity, due to a characteristically low parasitaemia in infected individuals. Differentiation of infections by Trypanosoma brucei rhodesiense (causes acute disease) and T. b. gambiense (causes chronic disease) is essential, as the two forms of disease have different treatment regimens. In the present work, loop-mediated isothermal amplification (LAMP) of DNA was successfully used to detect T. b. rhodesiense, with a sensitivity of up to one trypanosome/ml of blood. The LAMP test was efficient and robust, and results were obtained within 35 min. Amplification was possible when a water bath was used to maintain the temperature at isothermal conditions (60-65°C), and results could be read by visual observation of colour change. These findings have increased the prospects for ...
http://journals.plos.org/plosntds/article/comments?id=10.1371/journal.pntd.0000147&imageURI=info:doi/10.1371/journal.pntd.0000147.g004
*  Attomolar Zika virus oligonucleotide detection based on loop-mediated isothermal amplification and AC susceptometry
Because of the serological cross-reactivity among the flaviviruses, molecular detection methods, such as reverse-transcription polymerase chain reaction (RT-PCR), play an important role in the recent Zika outbreak. However, due to the limited sensitivity, the detection window of RT-PCR for Zika viremia is only about one week after symptom onset. By combining loop-mediated isothermal amplification (LAMP) and AC susceptometry, we demonstrate a rapid and homogeneous detection system for the Zika virus oligonucleotide. Streptavidin-magnetic nanoparticles (streptavidin-MNPs) are premixed with LAMP reagents including the analyte and biotinylated primers, and their hydrodynamic volumes are dramatically increased after a successful LAMP reaction. Analyzed by a portable AC susceptometer, the changes of the hydrodynamic volume are probed as Brownian relaxation frequency shifts, which can be used to quantify the Zika virus oligonucleotide. The proposed detection system can recognize 1 aM synthetic Zika ...
http://ri.diva-portal.org/smash/record.jsf?pid=diva2:1131695
*  Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay...
A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤1.8 M, compared with a PCR assay that was functional at concentrations of ,100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.. ...
http://researchonline.ljmu.ac.uk/7560/
*  Loop‐Mediated Isothermal Amplification for Detection of Nucleic Acids - Current Protocols
Sequence‐specific isothermal nucleic acid amplification techniques are ideally suited for use in molecular diagnostic applications because they do not require thermal cycling equipment and the reactions are typically fast
http://www.currentprotocols.com/WileyCDA/CPUnit/refId-mb1514.html
*  Detection of bacteria carrying the stx2 gene by in situ loop-mediated isothermal amplification.
Abstract A new in situ DNA amplification technique for microscopic detection of bacteria carrying a specific gene is described. Loop-mediated isothermal amplification (LAMP) was us..
https://www.omicsonline.org/references/detection-of-bacteria-carrying-the-stx2-gene-by-in-situ-loopmediated-isothermal-amplification-1251132.html
*  African trypanosomiasis: Sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification ...
Control of human African trypanosomiasis (HAT) is dependent on accurate diagnosis and treatment of infected patients. However, sensitivities of tests in routine use are unsatisfactory, due to the characteristically low parasitaemias in naturally infected individuals. We have identified a conserved sequence in the repetitive insertion mobile element (RIME) of the sub-genus Trypanozoon and used it to design primers for a highly specific loop-mediated isothermal amplification (LAMP) test. The test was used to analyse Trypanozoon isolates and clinical samples from HAT patients. The RIME LAMP assay was performed at 62 °C using real-time PCR and a water bath. DNA amplification was detectable within 25 min. All positive samples detected by gel electrophoresis or in real-time using SYTO-9 fluorescence dye could also be detected visually by addition of SYBR Green I to the product. The amplicon was unequivocally confirmed through restriction enzyme NdeI digestion, analysis of melt ...
http://researchrepository.murdoch.edu.au/id/eprint/8905/
*  Loop-mediated isothermal amplification (LAMP) for detection of the red ring nematode, Bursaphelenchus cocophilus » Brill...
As a first step in developing a quick, accurate and simple method for the diagnosis of red ring disease, the loop-mediated isothermal amplification (LAMP)-based identification procedure was applied to the causative agent, Bursaphelenchus cocophilus. Two LAMP primer sets were designed using two loci of ribosomal RNA genes, i.e., D2-D3 expansion segments of the large subunit (D2-D3 LSU), and internal transcribed spacers (ITS). Within those two sets of primers, the D2-D3 LSU primer set successfully yielded amplicons from B. cocophilus nematode lysate prepared from 3-year-old DESS-fixed specimens. The specificity of the primers was examined using 18 species of confamilial Aphelenchoididae nematodes and primer sensitivity was tested using a diluted series of B. cocophilus lysate. The primer set did not amplify the DNA from other aphelenchoidids, and sensitivity was achieved by '1:100 diluted' B. cocophilus DNA (roughly 1/1500 of total DNA from a single third-stage juvenile).
http://booksandjournals.brillonline.com/content/journals/10.1163/15685411-00003069
*  Folia Parasitologica: Urine sample used for detection of Toxoplasma gondii infection by loop-mediated isothermal amplification ...
In this study, a loop-mediated isothermal amplification (LAMP) assay was established to detect Toxoplasma gondii DNA in mice infected with T. gondii PRU strain. This LAMP assay was based on the sequence of highly repetitive B1 gene. The detect...
http://folia.paru.cas.cz/artkey/fol-201201-0004_Urine_sample_used_for_detection_of_Toxoplasma_gondii_infection_by_loop-mediated_isothermal_amplification_LAMP.php
*  Performance of loop-mediated isothermal amplification (LAMP) for the diagnosis of malaria among malaria suspected pregnant...
Laboratory diagnosis has relied on the identification of malaria parasites and parasite antigens in peripheral blood using Giemsa-stained microscopy or rapid diagnostic tests (RDTs) which lack analytical and clinical sensitivity. The aim of this study was to evaluate the performance of loop-mediated isothermal amplification (LAMP) for the diagnosis of malaria among malaria suspected pregnant women in Northwest Ethiopia. The authors conclude that this study showed higher sensitivity of LAMP compared to microscopy and RDT for the detection of malaria in pregnancy. Increased sensitivity and ease of use with LAMP in point-of-care testing for malaria in pregnancy was noted. LAMP warrants further evaluation in intermittent screening and treatment programmes in pregnancy.. ...
http://hesp-news.org/2017/01/27/performance-of-loop-mediated-isothermal-amplification-lamp-for-the-diagnosis-of-malaria-among-malaria-suspected-pregnant-women-in-northwest-ethiopia/
*  Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a diagnostic tool of Toxoplasma gondii...
Vet Parasitol. 2013 Feb 18;192(1-3):98-103. doi: 10.1016/j.vetpar.2012.10.010. Epub 2012 Oct 23. Research Support, Non-U.S. Gov't
https://www.ncbi.nlm.nih.gov/pubmed/23146414
*  Development of a quadruplex loop-mediated isothermal amplification assay for field detection of four Vibrio species associated...
In aquaculture, vibriosis is known as a major bacterial disease in fish culture systems and can cause considerable loss in terms of production and processing (Toranzo et al. 2005). Many Vibrio species have been recognized as fish pathogens that can cause infection with various symptoms. For example, Vibrio scophthalmi infection results in hemorrhage on fish body surface and inner surface of the abdomen, severe enteritis, and ascites (Qiao et al. 2012); Vibrio ichthyoenteri infection cause opaque intestines and necrotizing fasciitis with high mortality rates (Ishimaru et al. 1996; Lee et al. 2012); Vibrio parahaemolyticus infection causes diseases not only in fish, shrimp, oysters, and mussels, etc. (Montilla et al. 1994; Quintoil et al. 2007), but also is important in public health and causes gastrointestinal disorders in humans who ingest contaminated fish and shellfish (Kubota et al. 2008; Iwamoto et al. 2010); and Vibrio vulnificus has been associated with vibriosis outbreaks in fish and ...
https://springerplus.springeropen.com/articles/10.1186/s40064-016-2760-x
*  Frontiers | Evaluation of Nucleic Acid Isothermal Amplification Methods for Human Clinical Microbial Infection Detection |...
Battling infection is a major healthcare objective. Untreated infections can rapidly evolve toward the condition of sepsis in which the body begins to fail and resuscitation becomes critical and tenuous. Identification of infection followed by rapid antimicrobial treatment are primary goals of medical care, but precise identification of offending organisms by current methods is slow and broad spectrum empirical therapy is employed to cover most potential pathogens. Current methods for identification of bacterial pathogens in a clinical setting typically require days of time, or a four- to eight-hour growth phase followed by DNA extraction, purification and PCR-based amplification. We demonstrate rapid (70-120 minutes) genetic diagnostics methods utilizing loop-mediated isothermal amplification (LAMP) to test for 15 common infection pathogen targets, called the Infection Diagnosis Panel (In-Dx). The method utilizes filtration to rapidly concentrate bacteria in sample matrices ...
https://www.frontiersin.org/articles/10.3389/fmicb.2017.02211/full
*  A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION™ nanopore sequencer | BMC...
In this study, we constructed a novel tool for the identification of six Plasmodium parasites, including two P. ovale subspecies, using MinION™ portable sequencer and the LAMP method. We demonstrated that the method can achieve a comprehensive diagnosis of malaria even in resource-limited endemic regions. In particular, the LAMP method employs a set of four specially designed primers that recognize six distinct sequences on the target DNA, and relies on an auto-cycling procedure under isothermal conditions. In addition, our method enabled rapid library preparation (within 30 min), with multiplex sequencing and streaming analysis of real-time sequencing data via the Rapid Barcoding Sequencing Kit. Therefore, our LAMP method combined with the MinION™ sequencer would be convenient to use in various clinical settings.. Recently, Loopamp™ MALARIA Pan/Pf Detection Kit (Eiken Chemical) has become commercially available for the diagnosis of malaria by the LAMP method. This LAMP kit has good ...
https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-017-2718-9/
*  GENETIC TEST SYSTEM - Patent application
0002] Nucleic acid amplification technologies, such as a polymerase chain reaction (hereinafter, referred to as "PCR") that requires a temperature change in reaction liquid amplification and detection processes and a loop mediated isothermal amplification method (hereinafter, referred to as "LAMP method") that does not require a temperature change in reaction liquid amplification and detection processes, have been used for amplification and quantification of nucleic acids contained in samples originating in living organisms. For nucleic acid amplification, PCR requires periodically changing a sample temperature in typically about two to three temperature regions. For example, a typical PCR is performed as follows: the sample temperature is heated up to 94° C. to separate the double strands, followed by ...
http://www.patentsencyclopedia.com/app/20140193893
*  Plus it
A47 Recently, GD-100 (SYSMEX, Kobe, Japan) has been released. It rapidly can detect and quantify mRNA by one-step nucleic acid amplification (OSNA). Loop-mediated isothermal amplification (LAMP) is performed and turbidity derived from magnesium pyrophosphate formation with LAMP can be monitored in OSNA. LAMP originally was reported in 2000 as a novel nucleic acid amplification method that uses only one type of enzyme and yields 109 of its target in less than an hour. In this study, we compared the sensitivity of OSNA using GD-100 versus conventional real-time RT-PCR, using five colon cancer cell lines. We then applied OSNA to detect micro-metastases in 1) lymph nodes (LNs) from patients with advanced colorectal adenocarcinomas; and 2) LNs submitted for frozen diagnosis from patients with several malignancies. Our goal was to develop a more efficient system to detect LN metastases than ...
http://clincancerres.aacrjournals.org/content/12/19_Supplement/A47