3T3 cell line slides ( mouse: embryo ) from GeneTex,Mouse NIH / 3T3 cells were cultured in DMEM with 4 mM L-glutamine and ... In order to keep the antigens in their native forms, cells were fixed in acetone-methanol. The cells are arrayed on a 12-well ( ... Mouse NIH / 3T3 cells were cultured in DMEM with 4 mM L-glutamine and harvested at the log phase of growth. In order to keep ... A - 20 cell line slides ( mouse: B lymphocyte; reticulum cell sarcoma ) from GeneTex. 8. 293 cell line slides ( human: kidney; ...
Expression of an exogenous eukaryotic DNA methyltransferase gene induces transformation of NIH 3T3 cells. J Wu, J P Issa, J ... Expression of an exogenous eukaryotic DNA methyltransferase gene induces transformation of NIH 3T3 cells ... Expression of an exogenous eukaryotic DNA methyltransferase gene induces transformation of NIH 3T3 cells ... Expression of an exogenous eukaryotic DNA methyltransferase gene induces transformation of NIH 3T3 cells ...
NIH 3T3 whole cell lysate (TPA stimulated) Lysates datasheet (ab14878). Abcam offers quality products including antibodies, ... NIH 3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to ... NIH 3T3 whole cell lysate (TPA stimulated). See all NIH 3T3 lysates. ... Cell line: NIH/3T3 (Mouse embryonic fibroblast).. Cells were cultured in medium supplemented with 50 ng/ml TPA (phorbol, 12- ...
EWS/FLI1-induced manic fringe renders NIH 3T3 cells tumorigenic [1].. *NIH 3T3 cells transfected with the cDNA of this protein ... Gene context of NIH 3T3 Cells. *NIH 3T3-EGFR clonal lines, which expressed the EGF at 500- to 1000-fold levels over control NIH ... Biological context of NIH 3T3 Cells. *Transformants of NIH 3T3 cells induced by transfection with MMTV LTR-p21 hybrid DNA have ... Disease relevance of NIH 3T3 Cells. *Overexpression of MFNG in NIH 3T3 cells renders them tumorigenic in mice with severe ...
... nih 3t3 cells include Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells, Expression of ... Electroporation of Adherent Cells on Indium-Tin Oxide, The Submerged Printing of Cells onto a Modified Surface Using a ... Gradient Strain Chip for Stimulating Cellular Behaviors in Cell-laden Hydrogel, Real-Time Impedance-based Cell Analyzer as a ... A Cell-based Assay to Determine the Oncogenic Potential of a Gene, A Functional Assay for Gap Junctional Examination; ...
When NIH 3T3 cells were depleted of their glucosylceramide based glycosphingolipid mass, the caveolar structure remained intact ... Caveolar structure and protein sorting are maintained in NIH 3T3 cells independent of glycosphingolipid depletion.. Shu L1, Lee ... When the GPI-linked protein B61 was inducibly expressed in these cells, sorting to caveolar membranes occurred normally, even ... Glycosphingolipids have been proposed to be critical components of clustered lipids within cell membranes that serve as rafts ...
Homocysteine and alpha-lipoic acid regulate p44/42 MAP kinase phosphorylation in NIH/3T3 cells.. Shi SS1, Day RM, Halpner AD, ... phosphorylation were examined in NIH/3T3 fibroblasts. Cells grown in serum-containing media had constitutive levels of MAPK ... Treatment of cells with 20 microM Hcy for 0-60 min resulted in a transient enhancement of MAPK phosphorylation. In contrast, 20 ... The effect of alphaLA appears to be serum-dependent because Hcy or alphaLA treatment of serum-deprived cells activated MAPK ...
Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... weve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ... To give you confidence in the health of your cells every step of the way, ... Embryonic mouse fibroblast; Properties: contact inhibition; transformation; transfection; cell biology; ability to ...
... posted in Cell Biology: Im trying to transfect NIH3t3 with Calcium phosphate but the efficiency is relly low. Does someone ... Do you need to use calcium phosphate? Ive had luck in the past using Lipofectamine on NIH 3T3 cells. The efficiency wasnt all ... Im trying to transfect NIH3t3 with Calcium phosphate but the efficiency is relly low. Does someone know a way to optimize it ... Im trying to transfect NIH3t3 with Calcium phosphate but the efficiency is relly low. Does someone know a way to optimize it ...
The exposure of NIH/3T3 cells to nickel-refining fumes significantly reduced cell viability and induced cell apoptotic death in ... In our research, NIH/3T3 cells were exposed to nickel-refining fumes at the concentrations of 0, 6.25, 12.50, 25, 50 and 100 μg ... DNA damage and apoptosis of NIH/3T3 cells. ... for 24 h. Cell viability, cell apoptosis, reactive oxygen ... The exposure of NIH/3T3 cells to nickel-refining fumes significantly reduced cell viability and induced cell apoptotic death in ...
Highest nontoxic concentration for cultured NIH 3T3 cells assessed by MTT staining.. ... NIH NLMU.S. National Library of Medicine NCBINational Center for Biotechnology Information ...
Time-lapse video recording and cytogenetic analysis were used to determine the sensitivity of NIH3T3 cells to the RNA ... We have analysed the role of RNA polymerase II-dependent transcription in cell cycle progression. ... 3T3 Cells / drug effects. Amanitins / pharmacology. Animals. Cycloheximide / pharmacology. G1 Phase / drug effects*. Genes, fos ... Time-lapse video recording and cytogenetic analysis were used to determine the sensitivity of NIH3T3 cells to the RNA ...
The following studies compared traditional NIH 3T3 fibroblasts functions on XanoMatrix scaffolds to standard tissue culture ... In this manner, this study supports that cell culture dishes which mimic features of natural tissues should be continually ... Results found significantly greater fibroblast adhesion and proliferation on XanoMatrix cell culture dishes which mimic the ... and studying cell behavior in vitro. However, increasingly it has been observed that mimicking natural tissue properties (such ...
Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ... Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ... Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ... Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ...
"NIH 3T3 Cells" by people in this website by year, and whether "NIH 3T3 Cells" was a major or minor topic of these publications ... "NIH 3T3 Cells" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... NIH 3T3 Cells*NIH 3T3 Cells. *3T3 Cell, NIH. *Cell, NIH 3T3 ... 3T3 Cells [A11.251.210.700]. *NIH 3T3 Cells [A11.251.210.700. ... A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for ...
Transformation of normal NIH-3T3 cells into highly oncogenic and invasive cancer cells by MBD2 overexpression. Stephen Andrews ... Transformation of normal NIH-3T3 cells into highly oncogenic and invasive cancer cells by MBD2 overexpression ... Transformation of normal NIH-3T3 cells into highly oncogenic and invasive cancer cells by MBD2 overexpression ... Transformation of normal NIH-3T3 cells into highly oncogenic and invasive cancer cells by MBD2 overexpression ...
To establish cell lines with stable expression of GFP-LC3, control NIH/3T3 cells and NIH/3T3 cells overexpressing IRS-1 were ... Establishment of NIH/3T3 cells with stable overexpression of functional IRS-1. (A) Wild-type NIH/3T3 cells were serum starved ... Establishment of NIH/3T3 cells overexpressing functional IRS-1 We chose NIH/3T3 cells as an experimental model to investigate ... we established NIH/3T3 cells with stable overexpression of IRS-1. The levels of total IRS-1 in both the control NIH/3T3 cells ...
... detergent-extractable protein kinase C activity compared with control NIH 3T3 cells. However, TPA treatment of HL-60 cells does ... Phorbol Ester-mediated Association of Protein Kinase C to the Nuclear Fraction in NIH 3T3 Cells. Thomas P. Thomas, Harvinder S. ... Treatment of intact NIH 3T3 cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) causes a rapid redistribution (stabilization ... Phorbol easter (TPA) treatment of NIH 3T3 cells induces the tight association of protein kinase C to the nucleus; this tightly ...
Fast-forward protocol for transient transfection of NIH/3T3 cells in 96-well plates using Effectene® Transfection Reagent - (EN ...
Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes.. D Shalloway, P J Johnson, E O Freed, D ... Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. ... Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. ... Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. ...
... mouse embryonic fibroblast cell line) toxicity Measured in Cell-Based System Using Plate Reader - 2017-02_Inhibitor_Dose_ ... NIH NLMU.S. National Library of Medicine NCBINational Center for Biotechnology Information ...
The intrinsic radiation resistance (D0) of NIH 3T3 cells was markedly and significantly increased by transformation with ras ... The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation ... The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation ... The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation ...
NIH 3T3 cells and reveal a sequence of signaling events required for cell cycle progression in proliferating NIH 3T3 cells. ... Cell age and cell cycle position. Asynchronous NIH 3T3 cells were followed for 20 h in time lapse, with the last 30 min in the ... In a cell with a relatively short G1 phase, such as the NIH 3T3 cells studied here, Ras must become activated during G2phase to ... Cellular Ras and Cyclin D1 Are Required during Different Cell Cycle Periods in Cycling NIH 3T3 Cells. Masahiro Hitomi, Dennis W ...
Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ... Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ... Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ... Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ...
Cell culture and saline solutions.. NIH-3T3 cells were maintained in RPMI-1640 medium (Sigma) supplemented with 10% FCS and 1% ... Effect of hypotonic stress and cell swelling on the activation of PDGFR-β in NIH-3T3 cells. A: phase-contrast microscopy of ... Lang F, Lepple-Wienhues A, Szabó I, Siemen D, Gulbins E. Cell volume in cell proliferation and apoptotic cell death. In: Cell ... Effect of osmotic stress on the level of phosphorylation of JNK1/2 in NIH-3T3 cells. A and B: SDS-PAGE and Western blot ...