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*  WikiGenes - NIH 3T3 Cells
EWS/FLI1-induced manic fringe renders NIH 3T3 cells tumorigenic [1].. *NIH 3T3 cells transfected with the cDNA of this protein ... Gene context of NIH 3T3 Cells. *NIH 3T3-EGFR clonal lines, which expressed the EGF at 500- to 1000-fold levels over control NIH ... Biological context of NIH 3T3 Cells. *Transformants of NIH 3T3 cells induced by transfection with MMTV LTR-p21 hybrid DNA have ... Disease relevance of NIH 3T3 Cells. *Overexpression of MFNG in NIH 3T3 cells renders them tumorigenic in mice with severe ...
https://www.wikigenes.org/e/mesh/e/22574.html
*  NIH 3T3 whole cell lysate (TPA stimulated) (ab14878) | Abcam
NIH 3T3 whole cell lysate (TPA stimulated) Lysates datasheet (ab14878). Abcam offers quality products including antibodies, ... NIH 3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to ... NIH 3T3 whole cell lysate (TPA stimulated). See all NIH 3T3 lysates. ... Cell line: NIH/3T3 (Mouse embryonic fibroblast).. Cells were cultured in medium supplemented with 50 ng/ml TPA (phorbol, 12- ...
https://www.abcam.com/nih-3t3-whole-cell-lysate-tpa-stimulated-ab14878.html
*  NIH3t3 transfection optimization - Cell Biology - BioForum
... posted in Cell Biology: I'm trying to transfect NIH3t3 with Calcium phosphate but the efficiency is relly low. Does someone ... Do you need to use calcium phosphate? I've had luck in the past using Lipofectamine on NIH 3T3 cells. The efficiency wasn't all ... I'm trying to transfect NIH3t3 with Calcium phosphate but the efficiency is relly low. Does someone know a way to optimize it ... I'm trying to transfect NIH3t3 with Calcium phosphate but the efficiency is relly low. Does someone know a way to optimize it ...
http://www.protocol-online.org/forums/topic/14969-nih3t3-transfection-optimization/
*  nih 3t3, 3t3-l1 Cells | Thermo Fisher Scientific - US
Every Step of the Way, a Wide Range of Cell Health Products. Maintaining healthy cells is the key to experimental success and ... we've highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No ... To give you confidence in the health of your cells every step of the way, ... Embryonic mouse fibroblast; Properties: contact inhibition; transformation; transfection; cell biology; ability to ...
http://www.thermofisher.com/us/en/home/technical-resources/cell-lines/n/cell-lines-detail-334.html
*  AID 143821 - Highest nontoxic concentration for cultured NIH 3T3 cells assessed by MTT staining. - PubChem
Highest nontoxic concentration for cultured NIH 3T3 cells assessed by MTT staining.. ... NIH NLMU.S. National Library of Medicine NCBINational Center for Biotechnology Information ...
https://pubchem.ncbi.nlm.nih.gov/bioassay/143821
*  The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation | Science
The intrinsic radiation resistance (D0) of NIH 3T3 cells was markedly and significantly increased by transformation with ras ... The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation ... The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation ... The ras oncogenes increase the intrinsic resistance of NIH 3T3 cells to ionizing radiation ...
https://science.sciencemag.org/content/239/4840/645?ijkey=e99c1697efeec7f82977f897cd2c4cb6d5b7fe59&keytype2=tf_ipsecsha
*  Transformation of normal NIH-3T3 cells into highly oncogenic and invasive cancer cells by MBD2 overexpression | Cancer Research
Transformation of normal NIH-3T3 cells into highly oncogenic and invasive cancer cells by MBD2 overexpression. Stephen Andrews ... Transformation of normal NIH-3T3 cells into highly oncogenic and invasive cancer cells by MBD2 overexpression ... Transformation of normal NIH-3T3 cells into highly oncogenic and invasive cancer cells by MBD2 overexpression ... Transformation of normal NIH-3T3 cells into highly oncogenic and invasive cancer cells by MBD2 overexpression ...
http://cancerres.aacrjournals.org/content/67/9_Supplement/3925
*  Phorbol Ester-mediated Association of Protein Kinase C to the Nuclear Fraction in NIH 3T3 Cells | Cancer Research
... detergent-extractable protein kinase C activity compared with control NIH 3T3 cells. However, TPA treatment of HL-60 cells does ... Phorbol Ester-mediated Association of Protein Kinase C to the Nuclear Fraction in NIH 3T3 Cells. Thomas P. Thomas, Harvinder S. ... Treatment of intact NIH 3T3 cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) causes a rapid redistribution (stabilization ... Phorbol easter (TPA) treatment of NIH 3T3 cells induces the tight association of protein kinase C to the nucleus; this tightly ...
http://cancerres.aacrjournals.org/content/48/7/1910
*  Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. | Molecular and Cellular Biology
Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes.. D Shalloway, P J Johnson, E O Freed, D ... Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. ... Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. ... Transformation of NIH 3T3 cells by cotransfection with c-src and nuclear oncogenes. ...
https://mcb.asm.org/content/7/10/3582
*  Cellular Ras and Cyclin D1 Are Required during Different Cell Cycle Periods in Cycling NIH 3T3 Cells | Molecular and Cellular...
NIH 3T3 cells and reveal a sequence of signaling events required for cell cycle progression in proliferating NIH 3T3 cells. ... Cell age and cell cycle position. Asynchronous NIH 3T3 cells were followed for 20 h in time lapse, with the last 30 min in the ... In a cell with a relatively short G1 phase, such as the NIH 3T3 cells studied here, Ras must become activated during G2phase to ... Cellular Ras and Cyclin D1 Are Required during Different Cell Cycle Periods in Cycling NIH 3T3 Cells. Masahiro Hitomi, Dennis W ...
https://mcb.asm.org/content/19/7/4623
*  Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated...
Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ... Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ... Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ... Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ...
http://www.biochemj.org/content/362/2/305
*  Effects of osmotic stress on the activity of MAPKs and PDGFR-β-mediated signal transduction in NIH-3T3 fibroblasts | Cell...
Cell culture and saline solutions.. NIH-3T3 cells were maintained in RPMI-1640 medium (Sigma) supplemented with 10% FCS and 1% ... Effect of hypotonic stress and cell swelling on the activation of PDGFR-β in NIH-3T3 cells. A: phase-contrast microscopy of ... Lang F, Lepple-Wienhues A, Szabó I, Siemen D, Gulbins E. Cell volume in cell proliferation and apoptotic cell death. In: Cell ... Effect of osmotic stress on the level of phosphorylation of JNK1/2 in NIH-3T3 cells. A and B: SDS-PAGE and Western blot ...
http://ajpcell.physiology.org/content/294/4/C1046
*  Microscopy - NIH 3t3 cells
Max Image from a series scan. Depth of section 6.56 µm. Nuclei are stained in blue, f-actin filaments in red and microtubule associated proteins in green ...
http://digitalcollections.stlawu.edu/collections/microscopy/nih-3t3-cells
*  Riboflavin Depletion Promotes Tumorigenesis in HEK293T and NIH3T3 Cells by Sustaining Cell Proliferation and Regulating Cell...
"Riboflavin Depletion Promotes Tumorigenesis in HEK293T and NIH3T3 Cells by Sustaining Cell Proliferation and Regulating Cell ... A) Cell cycle distribution of 293T R+, 293T R-, 3T3 R+, and 3T3 R- cells. (B) The percentages of cells in the G1, S, G2/M, and ... A) Cell cycle distribution of 293T R+, 293T R-, 3T3 R+, and 3T3 R- cells. (B) The percentages of cells in the G1, S, G2/M, and ... A) Cell cycle distribution of 293T R+, 293T R-, 3T3 R+, and 3T3 R- cells. (B) The percentages of cells in the G1, S, G2/M, and ...
https://www.deepdyve.com/lp/ou_press/riboflavin-depletion-promotes-tumorigenesis-in-hek293t-and-nih3t3-jxCrv0HI20
*  Antioxidant action and cytotoxicity on HeLa and NIH-3T3 cells of new quercetin derivatives - Semantic Scholar
But for acetylated esters a better cytotoxic activity was found on human cervical cancer cells HeLa than for the initial ... Modification of their physicochemical properties could herewith improve the action in cells. The prepared compounds were tested ... For this compound comparable cytotoxic action on non-cancer murine fibroblast cells was detected (IC50=16.5 µM). The obtained ... results indicate that appropriate lipophilization of the quercetin molecule could improve its cytotoxic action in cells, ...
https://www.semanticscholar.org/paper/Antioxidant-action-and-cytotoxicity-on-HeLa-and-of-Danihelov%C3%A1-Veverka/6e37e0fbd5e325826a199ccc3350d2f4f6fc40d5
*  IJERPH | Free Full-Text | Nickel-Refining Fumes Induced DNA Damage and Apoptosis of NIH/3T3 Cells via Oxidative Stress
The exposure of NIH/3T3 cells to nickel-refining fumes significantly reduced cell viability and induced cell apoptotic death in ... In our research, NIH/3T3 cells were exposed to nickel-refining fumes at the concentrations of 0, 6.25, 12.50, 25, 50 and 100 μg ... DNA damage and apoptosis of NIH/3T3 cells. ... for 24 h. Cell viability, cell apoptosis, reactive oxygen ... The exposure of NIH/3T3 cells to nickel-refining fumes significantly reduced cell viability and induced cell apoptotic death in ...
http://www.mdpi.com/1660-4601/13/7/629
*  Insulin receptor substrate-1 prevents autophagy-dependent cell death caused by oxidative stress in mouse NIH/3T3 cells.
To establish cell lines with stable expression of GFP-LC3, control NIH/3T3 cells and NIH/3T3 cells overexpressing IRS-1 were ... Establishment of NIH/3T3 cells with stable overexpression of functional IRS-1. (A) Wild-type NIH/3T3 cells were serum starved ... Establishment of NIH/3T3 cells overexpressing functional IRS-1 We chose NIH/3T3 cells as an experimental model to investigate ... we established NIH/3T3 cells with stable overexpression of IRS-1. The levels of total IRS-1 in both the control NIH/3T3 cells ...
http://www.biomedsearch.com/nih/Insulin-receptor-substrate-1-prevents/22788551.html
*  AID 493247 - NIH/3T3(mouse embryonic fibroblast cell line) toxicity Measured in Cell-Based System Using Plate Reader - 2017-02...
... mouse embryonic fibroblast cell line) toxicity Measured in Cell-Based System Using Plate Reader - 2017-02_Inhibitor_Dose_ ... NIH NLMU.S. National Library of Medicine NCBINational Center for Biotechnology Information ...
https://pubchem.ncbi.nlm.nih.gov/bioassay/493247
*  Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay | Science
Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ... Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ... Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ... Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay ...
http://science.sciencemag.org/content/222/4620/173
*  AKT1/PKBalpha kinase is frequently elevated in human cancers and its constitutive activation is required for oncogenic...
... was introduced into NIH3T3 cells. Overexpression of Myr-Akt in the stably transfected cells resulted in malignant phenotype, as ... Constitutively activated AKT1 transforms NIH3T3 cells. A: Western blot (top) and in vitro kinase assay (bottom) analyses of Akt ... B: The morphology of constitutively active Akt-transfected NIH3T3 cells (2) is more rounded and larger than WT-Akt-transfected ... 3T3 Cells. *Animals. *Blotting, Western. *Breast Neoplasms/enzymology. *Breast Neoplasms/pathology. *Cell Transformation, ...
https://www.ncbi.nlm.nih.gov/pubmed/11485901?dopt=Abstract
*  NIH 3T3 lysate (ab7179) | Abcam
... mouse embryonic fibroblast cell line) whole cell lysate. ab7179 has been validated in western blot. Abcam now offers a 12-month ... NIH 3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to ... NIH/3T3 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.. ... This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the ...
http://www.abcam.com/nih-3t3-whole-cell-lysate-ab7179.html
*  NIH/3T3 Cell Culture Media Preparation - LPDwiki
NIH/3T3 Cell Culture Media Preparation. From LPDwiki. About this protocol: This protocol outlines the steps and materials ... Important: The Biosafety Cabinet (BSC) must be used for the preparation of NIH/3T3 cell culture media. Please follow the ... Solution Name: NIH/3T3 Cell Culture Media (DMEM, 10% FBS, 1% Pen/Strep) ... Retrieved from "http://www.lpdwiki.com/index.php?title=NIH/3T3_Cell_Culture_Media_Preparation&oldid=9" ...
http://www.lpdwiki.com/index.php?title=NIH/3T3_Cell_Culture_Media_Preparation
*  Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated...
Ecdysone-inducible expression of oncogenic Ha-Ras in NIH 3T3 cells leads to transient nuclear localization of activated ... In addition we show that transient activation of the MAPK pathway is sufficient to drive chronic cell-cycle progression. We ... There is remarkable redundancy between the Cyclin-Cdk complexes that comprise the cell cycle machinery. None of the mammalian A ...
https://repository.icr.ac.uk/handle/internal/1926
*  Adhesion, proliferation and migration of NIH/3T3 cells on modified polyaniline surfaces
Results showed that the NIH/3T3 cell line was able to adhere, proliferate and migrate on the pristine polyaniline films as well ... Adhesion, proliferation and migration of NIH/3T3 cells on modified polyaniline surfaces. International Journal of Molecular ... Adhesion, proliferation and migration of NIH/3T3 cells on modified polyaniline surfaces. DSpace Repository. Login ... Adhesion, proliferation and migration of NIH/3T3 cells on modified polyaniline surfaces. ...
http://publikace.k.utb.cz/handle/10563/1006743