... mitogen-activated protein kinase signaling pathways in the enhanced phosphorylation of initiation factor 4E in NIH 3T3 cells. J ... Cell viability and cell cycle progression.. C2C12 myocytes were subcultured in six-well plates to 90% confluence. Some cells ... Treatment of cells with indinavir in 1% FBS medium for 2 days did not affect cell viability (76.0 ± 2.3 × 104 cells/well) ... Applications of Advanced Cell Culture in Cell Physiology: Co-culture, Organoids, Stem Cells: Submission deadline December 31, ...http://ajpcell.physiology.org/content/287/5/C1482
Kinetics of nuclear-cytoplasmic translocation of Foxo1 and Foxo3A in adult skeletal muscle fibers | Cell Physiology
In FL5.12 and NIH 3T3 cell lines, phosphorylated Foxo1 can be dephoshosphorylated by PP2A, a serine/threonine phosphatase, ... PP2A directly dephosphorylates Foxo1 in NIH T3T cells (37). The impressive decrease in nuclear Foxo1 in response to inhibition ... Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Cell 96: 857-868, 1999. ... 9272; Cell Signaling) and phosphospecific Akt antibody (no. 9271; Cell Signaling) were used and the membrane was then treated ...http://ajpcell.physiology.org/content/303/9/C977.most-read
Activation of MAP kinase is both necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells. ... Cell cycle analysis of EPO-treated cells indicated an increased percentage of cells in the S phase, whereas cell numbers in G0/ ... Whole cell extracts from stimulated and nonstimulated cells were prepared by suspending cells in 500 μl of lysis buffer (20 mM ... Applications of Advanced Cell Culture in Cell Physiology: Co-culture, Organoids, Stem Cells: Submission deadline December 31, ...http://ajpcell.physiology.org/content/295/2/C394
... by centrifugation and titered using NIH 3T3 cells (American Type Culture Collection). The titer was measured using flow ... A and B: cells were seeded at 1,000 cells/well (A) or 5,000 cells/well (B) in 96-well plates, were left uninfected or infected ... Applications of Advanced Cell Culture in Cell Physiology: Co-culture, Organoids, Stem Cells: Submission deadline December 31, ... Cell pellets were resuspended in growth media and plated on 75-cm2 culture flasks. Cells were allowed to adhere for 2-3 h in a ...http://ajpcell.physiology.org/content/300/1/C176
Effects of osmotic stress on the activity of MAPKs and PDGFR-β-mediated signal transduction in NIH-3T3 fibroblasts | Cell...
Cell culture and saline solutions.. NIH-3T3 cells were maintained in RPMI-1640 medium (Sigma) supplemented with 10% FCS and 1% ... Effect of hypotonic stress and cell swelling on the activation of PDGFR-β in NIH-3T3 cells. A: phase-contrast microscopy of ... Lang F, Lepple-Wienhues A, Szabó I, Siemen D, Gulbins E. Cell volume in cell proliferation and apoptotic cell death. In: Cell ... Effect of osmotic stress on the level of phosphorylation of JNK1/2 in NIH-3T3 cells. A and B: SDS-PAGE and Western blot ...http://ajpcell.physiology.org/content/294/4/C1046
Bcl-2 overexpression abolishes early calcium waving preceding apoptosis in NIH-3T3 murine fibroblasts. Biochem Biophys Res ... Applications of Advanced Cell Culture in Cell Physiology: Co-culture, Organoids, Stem Cells: Submission deadline December 31, ... Orrenius S, Zhivotovsky B, Nicotera P. Regulation of cell death: the calcium-apoptosis link. Nat Rev Mol Cell Biol 4: 552-565, ... Samali A, Zhivotovsky B, Jones D, Nagata S, Orrenius S. Apoptosis: cell death defined by caspase activation. Cell Death Differ ...http://ajpcell.physiology.org/content/296/5/C941