Recombinant Anti-CBX1 / HP1 beta antibody [EPR24242-78] KO Tested (ab270988)
ICC/IF: CBX1 KO HAP1, HeLa and NIH/3T3 cells. Flow Cyt: Wild-type HAP1, HeLa and NIH/3T3 cells. IP: HeLa and NIH/3T3 cells. ... ab270988 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270988 in NIH/3T3 whole cell ... whole cell lysate. Lane 4 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate. Lane 5 : NIH/3T3 (mouse ... WB: Wild-type HAP1, CBX1/HP1 beta knockout HAP1 , 293T, HeLa, NIH/3T3, MCF7, A431, ES-D3, C2C12, C6, RAW264.7, PC-12 whole cell ...
Recombinant Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] (ab32575)
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate. Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell ... Flow Cyt (intra): HeLa cells. ICC/IF: A431, C6, NIH/3T3 cells. ... A-431 whole cell lysate (ab30132) *NIH/3T3 whole cell lysate ( ... WB: A431, HeLa, C6, RAW264.7, PC-12, NIH/3T3 and MCF7 cell lysates. IHC-P: Human uterus, human smooth muscle and mouse smooth ... Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3(Mouse embryonic fibroblast) cells labeling alpha smooth muscle ...
Recombinant Anti-Catalase antibody [EPR20198] - Peroxisome Marker KO Tested (ab209211)
... whole cell lysate. Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate. Lane 3 : NIH/3T3 (Mouse ... Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and ... ICC/IF: C2C12 and NIH/3T3 cells. Flow Cyt (intra): C2C12 cells. ... NIH/3T3, HepG2, 293 and C2C12 whole cell lysates; Human fetal ... Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Catalase with ab209211 at 1/100 ...
Recombinant Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729) | Abcam
ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra): HeLa and NIH/3T3 cells. IP: HeLa whole cell lysate. ... whole cell lysate. Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate. Lysates/proteins at 10 µg per ... Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells ... Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling AKT1 + AKT2 with ab182729 at 1/500 ...
Recombinant Anti-Smad4 antibody [EPR22589-112] KO Tested (ab230815) | Abcam
... ab230815 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230815 in NIH/3T3 whole cell ... Flow Cyt (intra): WT HAP1 cells. ICC/IF: HeLa cell line treated with TGF-beta. IP: HeLa and NIH/3T3 whole cell lysates. ... Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate. Lysates/proteins at 20 µg per lane.. Secondary. All lanes : ... WB: HAP1, HeLa, HCT116, HepG2, RAW264.7, HEK-293 and NIH/3T3 whole cell lysates; human fetal lung, mouse embryo, mouse lung and ...
Anti-PMP70 antibody (ab3421) | Abcam
NIH/3T3, HMVEC, NS-1, P19, A549 whole cells. IHC-P: Human duodenum tissue; Flow Cyt: HepG2, 293T, and NIH-3T3 cells ... Flow cytometry analysis of PMP70 in NIH/3T3 (Mouse embryo fibroblast cell line) whole cells (green) compared to an isotype ... Immunofluorescent analysis of PMP70 (green) in NIH/3T3 (Mouse embryo fibroblast cell line) ells. The cells were permeabilized ... ICC/IF image of ab3421 stained human HeLa (Human epithelial adenocarcinoma cell line) cells. The cells were 4% PFA fixed (10 ...
Anti-V5 tag antibody [SV5-Pk1] (ab27671) | Abcam
Lanes 1-2 : Transfected NIH/3T3 cell lysate. Lane 3 : Not transfected NIH/3T3 cell lysate. Secondary. All lanes : HRP- ... Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Transfected S2 Cells expressing V5 Tagged protein) ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ... A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the ...
Anti-CENPB antibody KO Tested (ab25734) | Abcam
Mouse Cell (NIH/3T3 - CRL-1658 - ATCC (mouse fibroblast)). Permeabilization. Yes - 0.1%Triton X-100 in 1XPBS buffer ... whole cell lysate. Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. Lane 4 : Daudi ( ... Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat whole cell lysate (ab7899). Lane 3 : A- ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ...
Anti-FHL2 antibody (ab12328) | Abcam
Lane 2 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate. Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) ... WB: HeLa, TCMK-1 and NIH/3T3 whole cell lysates. IP: HeLa cells. IHC-P: Human breast and ovarian carcinoma tissue. ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ... ICC image of ab12328 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat ...
Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) | Abcam
Human T cell leukemia cell line from peripheral blood) whole cell lysate. Lanes 3 & 6 : NIH/3T3 (Mouse embryo fibroblast cell ... Lane 3 : NIH 3T3 (Mouse embryo fibroblast cell line) Whole Cell Lysate. Lane 4 : PC12 (Rat adrenal gland pheochromocytoma cell ... Lane 3 : HEK293 (Human epithelial cell line from embryonic kidney) cell lysate. Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell ... WB, ICC/IF: HeLa, Jurkat, A431, HEK293, NIH 3T3, PC12 cells. IHC-P: Human colon (FFPE), Rat colon (FFPE) tissue. Hela cells ...
Recombinant Anti-Bcl10 antibody [EPR26560-101] (ab302619) | Abcam
WB: Mouse spleen and skeletal muscle tissue lysates; A20 and NIH/3T3 whole cell lysates. IP: A20 whole cell lysate. ... Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate. Lysates/proteins at 20 µg per lane.. Secondary. All lanes : ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ... Lane 2: ab302619 IP in A20 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302619 in A20 whole cell ...
Recombinant Anti-Nesprin1/Syne-1 antibody [EPR14196] (ab192234) | Abcam
Lane 1 : RAW 264.7 cell lysate. Lane 2 : PC12 cell lysate. Lane 3 : NIH 3T3 cell lysate. Lysates/proteins at 10 µg per lane.. ... HeLa, 293, HACAT, RAW 264.7, PC12 and NIH 3T3 cell lysates; human kidney, human bladder transitional cell carcinoma and rat ... Lane 1 : HeLa cell lysate. Lane 2 : 293 cell lysate. Lane 3 : HACAT cell lysate. Lysates/proteins at 20 µg per lane.. Secondary ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ...
Recombinant Anti-MBD3 antibody [EPR9913] - BSA and Azide free KO Tested (ab240144)
Chromatin was prepared from NIH/3T3 cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ... Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, ... Cell and tissue imaging tools. Cellular and biochemical assays. By product type. Proteins and Peptides. Proteomics tools. ...
Recombinant Anti-STAT3 antibody [EPR787Y] KO Tested (ab68153) | Abcam
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate. Lane 4 : C2C12 (Mouse myoblast cell line) whole cell lysate. ... NIH/3T3, C2C12, and Raji cell lysates. IHC-P: Human brain tissue. ICC/IF: HeLa cells. Flow Cyt (intra): Raji cells. ChIC/CUT& ... Lane 1 : Wild-type HAP1 whole cell lysate. Lane 2 : STAT3 knockout HAP1 whole cell lysate. Lane 3 : HeLa whole cell lysate. ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ...
Recombinant Anti-p38 alpha/MAPK14 antibody [E229] KO Tested (ab170099) | Abcam
Lane 1 : NIH/3T3 cell lysate. Lane 2 : 3T3-L1 cell lysate. Lane 3 : PC-12 cell lysate. Lysates/proteins at 20 µg per lane.. ... WB: Jurkat, C6, NIH/3T3 or HeLa whole cell lysate (ab150035). ICC/IF: NIH/3T3 cell lysate. ... Lane 2: p38 knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg). Lane 4: Jurkat cell lysate (20 µg) Lanes 1 - 4 ... Lane 1: Wild-type HAP1 cell lysate (20 µg). Lane 2: p38 knockout HAP1 cell lysate (20 µg) Lane 3: HeLa cell lysate (20 µg). ...
Recombinant Anti-USP22 antibody [EPR18945] KO Tested (ab195289) | Abcam
... whole cell lysate. Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate. Lane 8 : NIH/3T3 (Mouse ... PC-12 and NIH/3T3 whole cell lysates; Mouse brain and spleen lysates; Rat brain and spleen lysates. IP: HeLa whole cell lysate ... whole cell lysate. Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. Lane 4 : HepG2 ( ... Lane 2: USP22 knockout HAP1 whole cell lysate (20 µg). Lane 3: HEK293 whole cell lysate (20 µg). Lane 4: HeLa whole cell lysate ...
Recombinant Anti-MEF2C antibody [EPR19089-202] - ChIP Grade KO Tested (ab211493)
Lane 2 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate. Lane 3 : C6 (rat glial tumor glial cell), whole cell lysate. ... ve control 1: ab197070 on jurkat (human T cell leukemia cell line from peripheral blood) cells.. -ve control 2: Jurkat cells ... WB: Human MEF2C recombinant protein; Raji, Jurkat, Ramos, Daudi, RAW264.7, NIH/3T3,C6, Wild-type THP-1 and Daudi whole cell ... Lane 1 : Wild-type THP-1 cell lysate. Lane 2 : MEF2C knockout THP-1 cell lysate. Lane 3 : Daudi cell lysate. Lane 4 : Jurkat ...
Recombinant Anti-Vinculin antibody [EPR8185] (ab129002) | Abcam
Lane 2 : NIH/3T3 cell lysate. Lysates/proteins at 10 µg per lane.. Secondary. All lanes : HRP goat anti-rabbit (H+L) at 1/1000 ... Lane 1 : PC3 cell lysate. Lane 2 : HeLa cell lysate. Lane 3 : U937 cell lysate. Lane 4 : K562 cell lysate. Lane 5 : HUVEC cell ... Cell junction , adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions ... Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E- ...
Recombinant Anti-Glucose Transporter GLUT1 antibody [EPR3915] KO Tested (ab115730)
WB: NIH/3T3, HepG2, HT-29, SW480, 3T3-L1 and PC-12 whole cell lysates. IHC-P: Rat kidney tissue; mouse liver tissue; human lung ... Lane 3 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates unboiled with 5% NFDM/TBST. Lane 4 : 3T3-L1 (Mouse embryonic ... Lane 1 : Wild-type HepG2 cell lysate. Lane 2 : LC2A1 knockout HepG2 cell lysate. Lane 3 : A549 cell lysate. Lane 4 : Jurkat ... ICC/IF: HepG2 cells and A549 (SLC2A1 knockout A549 cells used as a negative control) cells. Flow Cyt (intra): HeLa and Jurkat ...
Recombinant Anti-KDM2A antibody [EPR18602] KO Tested (ab191387) | Abcam
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate. Lane 4 : RAW 264.7 (Mouse macrophage cell line ... whole cell lysate. Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. Lane 3 : K562 ( ... WB: Human brain lysate; HeLa, Jurkat, K562, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates; rat brain lysate; mouse testis ... Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate. Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole ...
Anti-Nrf2 antibody KO Tested (ab137550) | Abcam
HeLa whole cell lysate (ab29545) *NIH/3T3 whole cell lysate (ab7179) * Recombinant Protein. *Recombinant Human Nrf2 protein ( ... Lane 1 : Wild-type A549 cell lysate. Lane 2 : NFE2L2 knockout A549 cell lysate. Lane 3 : HepG2 cell lysate. Lane 4 : Huh7 cell ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ... The cell line tested by ICC/IF is a cancer cell line.. In addition, phosphorylation of Ser-40 by PKC in response to oxidative ...