Murine DC were subjected to ECP, and inflammasome activation was triggered with ATP and nigericin. Both ATP and nigericin ... Murine bone marrow-derived DC were primed with 8-MOP (800 ng/ml) and UVA light (J/cm2) and activated with ATP or nigericin. IL- ... LPS, ATP, nigericin, apyrase, and z-YVAD-FMK were used at the following concentrations unless otherwise indicated: 250 pg/ml, 5 ... In the absence of LPS priming, nigericin or ATP was added after overnight incubation (12-16 h) after UVA irradiation, and IL-1β ...
Nigericin acts as an H+, K+, Pb2+ ionophore. Most commonly it is an antiporter of H+ and K+. In the past nigericin was used as ... Nigericin is an antibiotic derived from Streptomyces hygroscopicus. Its isolation was described in the 1950s, and in 1968 the ... The structure and properties of nigericin are similar to the antibiotic monensin. Commercially it is obtained as a byproduct, ... Steinrauf LK, Pinkerton M, Chamberlin JW (1968). "The structure of nigericin". Biochem. Biophys. Res. Commun. 33 (1): 29-31. ...
Nigericin acts as a potassium ionophore. The release of IL-1beta in response to nigericin has been demonstrated to be NALP3- ... Similar to ATP, nigericin induces a net decrease in intracellular levels of potassium which i ... Nigericin is a microbial toxin derived from Streptomyces hygroscopicus. ... Nigericin acts as a potassium ionophore. The release of IL-1β in response to nigericin has been demonstrated to be NALP3- ...
... nigericin can be used as a positive control to generate a robust caspase-1 activation response in a variety of cell lines. ... Nigericin. $41.00. Nigericin is a potent microbial toxin that acts as a potassium ionophore, inducing a net decrease in ... Nigericin can be utilized as a positive control in pyroptosis experiments.. Nigericin is a potent microbial toxin derived from ... 3. Use Nigericin at 1-20 µM to induce NLRP3 inflammasome activation in cells. For example, to use at 10 µM, dilute 500 µM ...
... and nigericin (B) increase the value of membrane potential but decrease the rate of ROS release. The same relative scale of ... fig5: Inorganic phosphate (A) and nigericin (B) increase the value of membrane potential but decrease the rate of ROS release. ... fig5: Inorganic phosphate (A) and nigericin (B) increase the value of membrane potential but decrease the rate of ROS release. ... At the time points indicated by arrows, the following additions were made: mitochondria (all traces), 100 nm nigericin (all ...
Nigericin sodium salt is an antibiotic from Streptomyces hygroscopicus that works by acting as an H+, KNigericin sodium salt (Synonyms: Sodium Nigericin). Cat. No.: HY-100381 Purity: ,98.0% Data Sheet SDS Handling Instructions ... Nigericin exhibits gteat toxicity for the HT29 and SW116 cell line with IC50 of 12.92±0.25 μmol and 15.86±0.18 μmol. Nigericin ... Nigericin inhibits migration and invasion of H460 lung cancer cells[1]. Nigericin (0.1-10 nM) has apparently a dual effect on ...
Nigericin effectively causes intracellular parasites to exit their host cell within 30 min of treatment with the drug. Our ... Nigericin effectively causes intracellular parasites to exit their host cell within 30 min of treatment with the drug. Our ... Nigericin effectively causes intracellular parasites to exit their host cell within 30 min of treatment with the drug. Our ... Nigericin effectively causes intracellular parasites to exit their host cell within 30 min of treatment with the drug. Our ...
Nigericin Intracellular calibration of the fluorescence response of cytosolic pH indicators is typically performed using the K+ ... This study also reported nigericin calibrations that yielded different pKa values (6.8 versus 7.4) in the kidney cortex and ... ionophore nigericin (N1495), which causes equilibration of intracellular and extracellular pH in the presence of a depolarizing ... in situ calibration of BCECFs fluorescence response is usually accomplished using 10-50 µM nigericin (N1495, see below) in the ...
Oh, JH, OMalley, KL, Krajewski, S, Reed, JC & Oh, YJ 1997, Bax accelerates staurosporine-induced but suppresses nigericin- ... Cleavage of poly(ADP-ribose)polymerase caused by nigericin was greatly attenuated in MN9D/Bax cells suggesting that, like Bcl-2 ... Cleavage of poly(ADP-ribose)polymerase caused by nigericin was greatly attenuated in MN9D/Bax cells suggesting that, like Bcl-2 ... Cleavage of poly(ADP-ribose)polymerase caused by nigericin was greatly attenuated in MN9D/Bax cells suggesting that, like Bcl-2 ...
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... arsenite and nigericin; LPS, RK-33 and nigericin; and LPS, RK-33, arsenite and nigericin. Representative blots (n = 2 ... Cells were stimulated with LPS and treated with nigericin or with nigericin and arsenite. ****P ≤ 0.0001 (two-way ANOVA) for ... Pam3CSK4 or Pam3CSK4 and nigericin (f), or poly(I:C) or poly(I:C) and nigericin (g). Representative blots (n = 2 biologically ... and nigericin; or poly(I:C), arsenite and nigericin. Representative blots (n = 2 biologically independent experiments). d, ...
Nig, nigericin (for calibration). (B) Realkalinization is blunted with both histamine and carbachol (each 100 μM) ... Intensity ratio data (490/440) were converted to pH values using the high-K+/nigericin calibration technique. ...
Squares, control; triangles, nigericin-treated sample. Nigericin (100 μM) was added to scratched leaves (see Materials and ... Fv/Fm values were 0.75 ± 0.05 and 0.7 ± 0.06 in the case of scratched leaves treated with water (control) and with nigericin, ... addition of the ionophore nigericin abolished most of the quenching in both cases (Fig. 1 C and D , triangles). ... of ΔpH in triggering transient NPQ is indicated by both its fast dark relaxation and sensitivity to the ionophore nigericin. ...
Nigericin may prove to be a novel therapeutic strategy that is effective in patients with metastatic EOC. ... Nigericin inhibits epithelial ovarian cancer metastasis by suppressing the cell cycle and epithelial−mesenchymal transition by ... Flow cytometry indicated that nigericin could induce cell cycle arrest at G0/G1 phase and promote cell apoptosis. Boyden ... Our results demonstrated that nigericin could inhibit EMT during cell invasion and metastasis through the canonical Wnt/β- ...
pH was clamped by nigericin-mediated K+/H+ antiport, whereas the voltage component of pmf was maintained. Addition of nigericin ... The effect of nigericin on cpYFP fluorescence dynamics in isolated mitochondria. (A) Mitochondria (20 μg) were suspended in a ... The effect of nigericin on cpYFP fluorescence dynamics in isolated mitochondria. (A) Mitochondria (20 μg) were suspended in a ... Nigericin also completely abolished cpYFP flashes in single isolated mitochondria monitored by CLSM (Figures 5B and 5C, and ...
T1 - The oxindole Syk inhibitor OXSI-2 blocks nigericin-induced inflammasome signaling and pyroptosis independent of potassium ... The oxindole Syk inhibitor OXSI-2 blocks nigericin-induced inflammasome signaling and pyroptosis independent of potassium ... The oxindole Syk inhibitor OXSI-2 blocks nigericin-induced inflammasome signaling and pyroptosis independent of potassium ... The oxindole Syk inhibitor OXSI-2 blocks nigericin-induced inflammasome signaling and pyroptosis independent of potassium ...
C, inhibition of MPP+ transport by nigericin. D, inhibition of MPP+ transport by 10 μm of known substrates (ethidium, ...
Streptomyces griseiniger produces nigericin. ed.-in-chief, George M. Garrity (2012). Bergeys manual of systematic bacteriology ...
nigericin. Poct. octanol-water partition coefficient of the nonionized drug. QN. quinine. PPR. propranolol. TFP. ...
A-F) NETosis rate in response to (A and D) PMA, (B and E) A23187 or nigericin and (C and F) C. albicans or GBS. (A-C) Primary ... B-D) NETosis rate and percentage of citrullinated cells in response to (B) PMA, (C) A23187 or nigericin and (D) C. albicans or ... Only PMA and nigericin require calcium to make NETs. PMA is a direct protein kinase C (PKC) activator which, in turn, leads to ... In contrast, nigericin did not induce any ROS production (Figure 3B). Opsonized C. albicans generated ROS (Figure 3C) peaked, ...
B) THP-1 cells were treated with DTA or nigericin (NGC). IL-1β production was measured using ELISA at 6 hours (n = 3/group). (C ... Nigericin-treated THP-1 macrophages were used as a positive control. Arrow indicates cleaved capase-1 N-terminal CARD domain. ( ... Nigericin (50 μM, Tocris Bioscience) or ATP (5 mM, Sigma-Aldrich) treatment was used as positive controls. Cell supernatants ... Nigericin (NGC) was used as positive control. Image is representative of 3 independent experiments. ( ...
Nigericin stimulation lasted 15 min. (H) LPS-primed BMDMs were treated with or without 20 μM 1,25VD for 3 h. NLRP3 ... D) Immunofluorescent staining for VDR and NLRP3 in LPS-primed BMDMs treated with or without nigericin. Scale bar, 10 μm. (E) ... When BMDMs were treated with 1,25(OH)2D3, NLRP3 inflammasome activation by LPS and nigericin was gradually inhibited, and IL-1β ... C) LPS-primed BMDMs were unstimulated or stimulated with nigericin for 30 min. Cell lysates were immunoprecipitated (IP) and ...
Each cell line was also incubated with NLRP3 agonist Nigericin sodium salt (NSS) and antagonist CY-09 separately (Selleck, ... 5 nm gemcitabine after GRC were treated with NLRP3 agonist Nigericin sodium salt (NSS) or antagonist CY-09. Epithelial-to- ...
Toxoplasma gondii: induction of egress by the potassium ionophore nigericin.. Fruth IA, Arrizabalaga G. ...
Sprede dannet ΔpH ved at tilføje 2 µM uncoupler (for eksempel nigericin). ACMA fluorescens signal skal hurtigt vende tilbage ... Forbered en 1 mM bestand af nigericin (eller nogen andre uncoupler) i ethanol. ...