*  Visinin-like protein 1 or VSNL1 Antibody
Research proven mouse monoclonal VSNL1 antibody. Visinin-like protein 1 is abundant in the nervous system and is found only in neurons. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury. Excellent for immunofluorescence and western blotting. IF and WB image in product description.
*  Neuronal calcium sensor proteins are direct targets of the insulinotropic agent repaglinide | Biochemical Journal
The NCS (neuronal calcium sensor) proteins, including neurocalcins, recoverins and visinin-like proteins are members of a family of Ca2+-sensitive regulators, each with three Ca2+-binding EF-hand motifs. In plants, lily CCaMK [chimaeric Ca2+/CaM (calmodulin)-dependent protein kinase] and its PpCaMK (Physcomitrella patens CCaMK) homologue are characterized by a visinin-like domain with three EF-hands. In the present study, in an effort to discover NCS antagonists, we screened a total of 43 compounds using Ca2+-dependent drug affinity chromatography and found that the insulinotropic agent repaglinide targets the NCS protein family. Repaglinide was found to bind to NCS proteins, but not to CaM or S100 proteins, in a Ca2+-dependent manner. Furthermore, the drug antagonized the inhibitory action of recoverin in a rhodopsin kinase assay with IC50 values of 400 μM. Moreover, repaglinide tightly ...
*  Volume 47, Number 1, 2015 | Journal of Alzheimer's Disease
Abstract: Calcium ions are crucial in the process of information transmission and integration in the central nervous system (CNS). These ions participate not only in intracellular mechanisms but also in intercellular processes. The changes in the concentration of Ca2+ ions modulate synaptic transmission, whereas neuronal activity induces calcium ion waves. Disturbed calcium homeostasis is thought to be one of the main features in the pathophysiology of Alzheimer's disease (AD), and AD pathogenesis is closely connected to Ca2+ signaling pathways. The effects of changes in neuronal Ca2+ are mediated by neuronal calcium sensor (NCS) proteins. It has been revealed that NCS proteins, with special attention to visinin-like protein 1 (VILIP-1), might have a connection to the etiology of AD. In the CNS, VILIP-1 influences the intracellular ...
*  Ca2+-Modulated ROS-GC1 Transduction Machinery in Isolated Transformed Retinal Ganglion Cells, RGC-5 | IOVS | ARVO Journals
Purpose: : Biochemical, structural and functional characterization of Ca2+-modulated cyclic GMP signaling pathway in the inner retinal neurons, the ganglion cells. Methods: : Immunohistochemical analyses, Western blotting, RT-PCR, Ca2+-imaging, patch clamp recordings and in vitro reconstitution experiments in conjunction with enzyme activity assays were employed. Results: : Ca2+-dependent membrane guanylate cyclase transduction machinery is expressed in RGC-5 cells. Through immunological and functional analyses ROS-GC1 has been identified as the enzyme component of the system. Two Ca2+ sensor proteins, neurocalcin delta and S100B, co-exist with ROS-GC1 and regulate its activity. Staining of RGC-5 cells with specific antibodies against neurocalcin d and ROS-GC1 shows positive reaction in the processes of the cells. Overexpression of ROS-GC1 promotes Ca2+ entry into RGC5 cells as evidenced by the markedly reduced time-to-peak ratio and increased amplitude of the response. ...
*  VSNL1 Antibody (monoclonal) (M01) - Mouse monoclonal antibody raised against a full length recombinant VSNL1. WB, E - Buy Now!...
VSNL1 Antibody (monoclonal) (M01), Mouse monoclonal antibody raised against a full length recombinant VSNL1. validated in WB, E (AT4523a), Abgent
*  anti-NCALD antibody | GeneTex
NCALD antibody (neurocalcin delta) for IHC-P, WB. Anti-NCALD pAb (GTX121264) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
*  "Regulation Of Neuronal Excitability: New Mechanisms For Slow Afterhyperpolarization A . . ." by Claudio Alberto Villalobos
One of the most characteristic features of pyramidal cells in the prefrontal cortex (PFC) is that they present a slow afterhyperpolarizing current (IsAHP) that plays a critical role in the regulation of neuronal excitability. This current is modulated by receptors acting via GΑq/11 G proteins, thus it is thought that neurotransmitters regulate neuronal excitability through the inhibition of this current. IsAHP is known to be mediated by calcium-activated potassium channels, however, neither the identity of the channel underlying this current nor its mechanism of activation are yet well understood. Recent reports have questioned a direct role of calcium in the activation of the channels underlying the IsAHP in hippocampus, suggesting the neuronal calcium sensor (NCS) protein hippocalcin as one of the plausible proteins involved in the triggering of IsAHP; ...
*  Neuronal calcium sensor-1 - Wikipedia
Neuronal calcium sensor-1 (NCS-1) also known as frequenin homolog (Drosophila) (freq) is a protein that is encoded by the FREQ gene in humans. NCS-1 is a member of the neuronal calcium sensor family, a class of EF hand containing calcium-myristoyl-switch proteins. NCS-1 regulates synaptic transmission, helps control the dynamics of nerve terminal growth, is critical for some forms of learning and memory in C. elegans and mammals, regulates corticohippocampal plasticity; and enhancing levels of NCS-1 in the mouse dentate gyrus increases spontaneous exploration of safe environments, potentially linking NCS-1 to curiosity. NCS-1 is a calcium sensor, not a calcium buffer (chelator); thus it is a high-affinity, low-capacity, calcium-binding protein. Frq can substitute for calmodulin in some situations. It is ...
*  Signaling Helix-dependent And -independent Modulation Of Photoreceptor Guanylate Cyclase ROS-GC1 By GCAPs | IOVS | ARVO Journals
Purpose: : To explain on structural basis the principles of Ca2+-modulated ROS-GC1 phototransduction process. Methods: : Gene deletion analysis, living cell fluorescence, co-immunoprecipitation and molecular modeling. Results: : The coiled coil signaling helix domain has been perceived critical for the signaling integrity of all receptor membrane guanylate cyclases. The puzzle is whether it is also critical for photoreceptor ROS-GC1 modulation. The study demonstrates that the GCAP components of ROS-GC1 transduction system, GCAP1 and GCAP2, sense and transmit signals in divergent modes. The target domains of these GCAPs reside on the opposite sides of the core catalytic domain of ROS-GC1. In vivo analyses utilizing in situ living cells fluorescence show that the signaling helix of ROS-GC1 is not required for GCAPs binding to ROS-GC1. Enzymatic analyses of various deletion mutants demonstrate that GCAP2 signals activation of ROS-GC1 in the absence of the signaling helix, thus the helix is not ...
*  New Alzheimer's marker strongly predicts mental decline - Healthcanal.com : Healthcanal.com
VILIP-1 appears to be a strong indicator of ongoing injury to brain cells as a result of Alzheimer's disease," says lead author Rawan Tarawneh, MD, now an assistant professor of neurology at the University of Jordan. "That could be very useful in predicting the course of the disease and in evaluating new treatments in clinical trials.". The study appears March 6 in Neurology.. VILIP-1 was originally identified as a potential indicator of brain cell damage in the laboratory of Jack Ladenson, PhD, the Oree M. Carroll and Lillian B. Ladenson Professor of Clinical Chemistry in Pathology and Immunology at Washington University. Scientists think VILIP-1 serves as a calcium sensor in brain cells. It is released into the cerebrospinal fluid when the cells are injured.. Tarawneh is a former postdoctoral research associate in the laboratory of David Holtzman, MD, the Andrew B. and Gretchen P. Jones Professor and head of Washington University's Department of Neurology. In an earlier ...
*  The Calcium Sensor for Slow Afterhyperpolarization | Science Signaling
Neurons in the brain protect themselves from excessive activity in part by a mechanism through which an influx of calcium ions activates potassium channels, which then cause hyperpolarization of the nerve cell, thus inhibiting further action potentials. The identity of this potassium channel is not known, but the current it conducts is called the slow afterhyperpolarization current (IsAHP). Tzingounis et al. identified a new key component of the mechanism by which calcium influences the channel: the neuronal calcium sensor family member known as hippocalcin. The authors interpreted results from kinetic analysis of IsAHP in the presence of various calcium buffers to indicate that IsAHP responded primarily to general changes in calcium concentration within the cell, as opposed to large rapid changes in calcium concentration in the immediate vicinity of calcium ...
*  Download Free Biology Books: Calcium Signalling and Disease: Molcular Pathology of Calcium
Description: This topic is a new entry in the area of cellular calcium signaling: yet, it now spans the entire area, with discoveries that cover both genetic and acquired pathologies, even offering glimpses in the direction of therapy. Cellular calcium homeostasis, and thus calcium signalling, is mainly regulated by membrane intrinsic proteins and calcium sensor proteins. Both classes may be involved in pathological processes that affect both human and animals, ranging from common and important diseases (e.g. migraine, diabetes, epilepsia, manic depression, infertility, various types of cancer, Alzheimer's disease, muscular dystrophy) to rare genetic conditions (e.g., a number of genetic heart conditions, autoimmune retinopathies, night blindness, hereditary amyloid polyneuropathy, malignant hyperthermia, cerebellar ataxia, atherothrombotic disease). Clearly, the topic has now become not ...
*  Negatively Calcium-Modulated Membrane Guanylate Cyclase Signaling System in the Rat Olfactory Bulb †
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*  Flagellar protein localization mediated by a calcium-myristoyl/palmitoyl switch mechanism | The EMBO Journal
Trypanosoma cruzi, a unicellular eukaryotic parasite, is the etiologic agent of Chagas' disease, a significant cause of morbidity and mortality in Central and South America (Brener, 1991). Currently, there are 19 countries in which the disease is endemic, with 16 million people infected with T.cruzi and 90 million more at risk (Molyneux, 1997). A 24 kDa flagellar calcium‐binding protein (FCaBP) is found exclusively in the flagellum of the parasite (Engman et al., 1989). FCaBP is a member of the EF‐hand calcium‐binding protein superfamily which possesses four putative binding motifs comprising 75% of the molecule (Engman et al., 1989; Wong et al., 1992; Godsel et al., 1995). FCaBP binds two calcium ions through the third and fourth EF‐hand domains (Maldonado et al., 1999). The calcium‐binding affinities of these sites place FCaBP in the calcium sensor group of EF‐hand proteins, which regulate ...
*  Effects of Ca2+, Mg2+, and myristoylation on guanylyl cyclase activating protein 1 structure and stability. - Semantic Scholar
Guanylyl cyclase activating protein 1 (GCAP1), a member of the neuronal calcium sensor (NCS) subclass of the calmodulin superfamily, confers Ca(2+)-dependent activation of retinal guanylyl cylcase (RetGC) during phototransduction in vision. Here we analyze the energetics of Ca(2+) and Mg(2+) binding to the individual EF-hands, characterize metal-induced conformational changes, and evaluate structural effects of myristoylation as studied by isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC), and nuclear magnetic resonance (NMR). GCAP1 binds cooperatively to Ca(2+) at EF3 and EF4 (DeltaH(EF3) = -3.5 kcal/mol, and DeltaH(EF4) = -0.9 kcal/mol) with nanomolar affinity (K(EF3) = 80 nM, and K(EF4) = 200 nM), and a third Ca(2+) binds entropically at EF2 (DeltaH(EF2) = 3.1 kcal/mol, and K(EF2) = 0.9 microM). GCAP1 binds functionally to Mg(2+) at EF2 (DeltaH(EF2) = 4.3 kcal/mol, and K(EF2) = 0.7 mM) required for RetGC activation. Ca(2+) ...
*  Plus it
Our results are the first to demonstrate enhanced endogenous calcium buffer capacity in the apical dendrites of CA1 hippocampal pyramidal neurons from aging animals. Buffer capacity in intact CA1 pyramidal neurons had not been directly measured, but was previously thought to decrease with age (Toescu and Verkhratsky, 2007; Kumar et al., 2009).. In this study, we did not identify the calcium buffer that is elevated with aging. More than 200 proteins that bind, and therefore buffer calcium, have been identified (Miller, 1991; Braunewell and Gundelfinger, 1999; Heizmann, 1999; Lewit-Bentley and Rety, 2000; Burgoyne et al., 2004; Schwaller, 2009). Some, such as parvalbumin, calbindin-D28, calretinin, hippocalcin, and visinin-like Ca2+-binding protein 2 (NVP2) are often reduced with normal aging (Villa et al., 1994; de Jong et al., 1996; Krzywkowski et al., 1996; Kishimoto et al., 1998; Furuta et al., 1999), suggesting that the increase in ...
*  PubMed Search Strategies: February 2013
Calcium-Binding Proteins"[mesh] OR Calsequestrin* OR Fetuin* OR "alpha-2-HS-Glycoprotein" OR Calcium-Sensing OR "calcium sensing" OR Calnexin* OR Calreticulin* OR Gelsolin* OR Osteocalcin* OR Osteonectin* OR "Leukocyte L1 Antigen Complex" OR Synaptotagmin* OR S100* OR SCP* OR EF-hand OR calmodulin* OR calcineurin* OR calpain* OR visinin-like* OR "ca2-binding" OR Syt OR "synaptic vesicle protein" OR CaMKII OR "ca/calmodulin-dependent" OR Annexin OR "C2 domain" OR "Calcium ATPase" OR "Calcium pump" OR Myosin OR "Troponin C" OR cabp* OR TOUCH3 OR TCH3 OR PINOID OR pid-binding OR PBP1 OR (("phosphorylase b" OR phosphorylaseb OR phosphorylase-b OR "myosin light chain" OR cam OR NAD OR cgmp-dependent) AND (kinase OR kinases)) OR "adenylate cyclase" OR "camp phosphodiesterase" OR "NO synthase" OR "inositol 3-kinase" OR "G protein coupled receptor" OR "g protein coupled receptors" OR dystrophin OR spectrin OR fodrin ...
*  Osmolarity sensor protein elisa and antibody
Shop Osmolarity sensor protein ELISA Kit, Recombinant Protein and Osmolarity sensor protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
*  Expatriate Indian develops computing grid - HindustanTimes.com - New Economy
New Economy, Internet Economy of India, E-commerce, E-governance, Internet, Industry, India, international, online, news, information technology, IT, cyber laws, dot coms, gadgets, consumer electronics, cell phones, pdas, handhelds, information appliances, digital cameras, Indian IT scenario, computing, personal technology, MP3, music centre, infotech, New Media, E-mail, analysis, broadband, wireless, Web, Wipro, Infosys, software, convergence, Microsoft, HP, Compaq, IBM, troubleshooting, hardware, networking, computers, ISP, gateway, VSNL, MTNL, Mantra Online, Satyam, MindTree, telecom, telecommunications
*  Re: Vlue of artemia nauplii
From: Raj ,ggrk at blr_vsnl.net.in, ,Subject: OSI Brine shrimp eggs , ,I have a large can of brine shrimp eggs by OSI which doesn't hatch.. ,other makes hatch under the same conditions. There is no date stamp ,on the can, I read somewhere that these eggs hatch better if they ,are ,older. ,So far never had a response from OSI, anyone know how to hatch these? ,I de-shell them and use sometimes but I rather have them live.. Hi Ray, As far as my knowledge goes, OSI harvested its Artemia cysts mainly from the Great Salt Lake in Utah in the past so we can carefully assume that you have bought Artemia franciscana GSL cysts. It speak for itself that you should not take my word for it and you should try to verify this with the original manufacturer. GSL-cysts used to have decent hatching characteristics but a medium shell life of about a year or so. Did you buy the can in India? A potential source of problems might be the storing of the cysts then, due to the high temperatures in your country. ...
*  Directorate of Sorghum Research
Name of the College: Karpagam Engineering College Address: Myleripalayam(po)Othakkalmandapam,Pollachi main road,Coimbaotre-32. City: Coimbatore State: Tamil Nadu Phone No.: 0422-2619041, 0422-2619042,43,46 University: Autonomous colleges Other Names for the College: Karpgam Institutions E-Mail ID: karpagam@vsnl.net Website: www.kce.ac.in More Details: Affiliated to Anna University Coimbatore,Accredited by NBA-AICTE,NewDElhiLocated in Myleriapalayam village.Karpagam institutions also includes-*Karpagam University(Near Echanari)*Karpagam College of Pharmacy(Myleripalayam)*Karpagam… ...
*  Immune Effector and Calcium Sensor: Roster of Synaptic Proteins Grows by Two | ALZFORUM
I am not certain if this is the proper venue for this discussion or how Dr. Baltimore feels about continuing this dialog. But, I appreciate this opportunity to tell our story-a tale for which it has been difficult to find a receptive audience. I would like to emphasize that my goal here is not to be confrontational but to uncover an explanation for the discrepancies; therefore, I simply want to explain our findings and how they contrast with the literature. I would also like to clarify that I take issue only with the whole-cell EMSAs of NF-κB in glutamate-treated neurons; activation of NF-κB in synaptosomes is not something we have analyzed. Still, synaptic activation of NF-κB would have to be considered irrelevant to nuclear transcription if one cannot demonstrate that its DNA-binding activity reaches the nucleus.. Let me simply reemphasize the fact that we do not see glutamate activation of NF-κB DNA binding in nuclear extracts made from nearly pure cultures of cerebral (i.e., neocortical ...
*  RCSB PDB for 1K9P
1K9P: Crystal structures of S100A6 in the Ca(2+)-free and Ca(2+)-bound states: the calcium sensor mechanism of S100 proteins revealed at atomic resolution.
*  Apoptosis-linked gene 2-interacting protein elisa and antibody
Shop Apoptosis-linked gene 2-interacting protein ELISA Kit, Recombinant Protein and Apoptosis-linked gene 2-interacting protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.