History of research on Arabidopsis thaliana - Wikipedia
Efficient transformation facilitated insertional mutagenesis as described further below. A. thaliana geneticists made important ... "Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana". Science. 301 (5633): 653-657. Bibcode:2003Sci...301..653A. doi: ... Krysan, Patrick J.; Young, Jeffery C.; Sussman, Michael R. (1999-12-01). "T-DNA as an Insertional Mutagen in Arabidopsis". The ... Laibachs student Erna Reinholz described mutagenesis of A. thaliana with X-ray radiation in 1945. George Rédei pioneered the ...
Exogenous DNA - Wikipedia
"Retroviral insertional mutagenesis: past, present and future". Oncogene. 24 (52): 7656-7672. doi:10.1038/sj.onc.1209043. ISSN ... Such insertion mutagenesis has been often used to identify many oncogenes in specific locations in tumor cells. Transfection is ... Mutagenesis Transfection Transduction Gene Transfer Sperm-mediated gene transfer (SMGT) Horizontal gene transfer "Exogenous DNA ... These DNA fragments may then become integrated into the chromosomes of nearby bacterial cells to undergo mutagenesis. This ...
Forward genetics - Wikipedia
Mutations can also be generated by insertional mutagenesis. Most often, insertional mutagenesis involves the use of transposons ... or insertional mutagenesis (e.g. transposable elements). Subsequent breeding takes place, mutant individuals are isolated, and ... This type of saturation mutagenesis within classical experiments was used to define sets of genes that were a bare minimum for ... Forward genetics involves the use of several mutagenesis processes to induce DNA mutations at random which may include: ...
Chromosome engineering - Wikipedia
For: Carlson, Corey M.; Largaespada, David A. (2005). "Insertional mutagenesis in mice: New perspectives and tools". Nature ...
Phenotype - Wikipedia
October 1999). "A large-scale insertional mutagenesis screen in zebrafish". Genes & Development. 13 (20): 2713-2724. doi: ... Vitaterna MH, Pinto LH, Takahashi JS (April 2006). "Large-scale mutagenesis and phenotypic screens for the nervous system and ...
Transposase - Wikipedia
Carlson CM, Largaespada DA (July 2005). "Insertional mutagenesis in mice: new perspectives and tools". Nature Reviews Genetics ...
Nottingham Arabidopsis Stock Centre - Wikipedia
2003). "Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana". Science. 301 (5633): 653-657. Bibcode:2003Sci...301..653A ... but also due to many new mutagenesis programs encouraged by the development of Arabidopsis as a major model organism ...
Short hairpin RNA - Wikipedia
This is advantageous as insertional mutagenesis is avoided. It is disadvantageous in that the progeny of the cell will lose the ... With this approach there is increased risk of insertional mutagenesis; however, the risk can be reduced by using an integrase- ...
Sleeping Beauty transposon system - Wikipedia
This process is referred to as insertional mutagenesis or transposon mutagenesis. When a gene is inactivated by insertion of a ... Carlson CM, Largaespada DA (July 2005). "Insertional mutagenesis in mice: new perspectives and tools". Nat. Rev. Genet. 6 (7): ...
Palindromic sequence - Wikipedia
Methylation makes the resistant gene inactive; this is called insertional inactivation or insertional mutagenesis. For example ...
Reelin - Wikipedia
In 1994, a new allele of reeler was obtained by means of insertional mutagenesis. This provided the first molecular marker of ... "Isolation of an allele of reeler by insertional mutagenesis". Proceedings of the National Academy of Sciences of the United ...
Genetically modified food - Wikipedia
Insertional mutagenesis is associated with a series of consequences; for example, mutations occur when existing genes of the ... "Organisms obtained by mutagenesis are GMOs and are, in principle, subject to the obligations laid down by the GMO Directive" ( ... "Heritable targeted mutagenesis in maize using a designed endonuclease". The Plant Journal. 61 (1): 176-87. doi:10.1111/j.1365- ...
Genome survey sequence - Wikipedia
... and insertional mutagenesis. Among these methods, insertional mutagenesis was proved to be very good and successful approach. ... At first, T-DNA was applied for insertional mutagenesis. However, using transposable element can bring more advantages. ...
Amita Sehgal - Wikipedia
discovered the sleepless gene in fruit flies through insertional mutagenesis. Mutations in the sleepless gene caused the flies ...
Conservative transposition - Wikipedia
This technique is used in transgenesis and insertional mutagenesis research fields. The Sleeping Beauty transposon system is an ...
Canalisation (genetics) - Wikipedia
causing massive transposon insertional mutagenesis that could explain the phenotypic diversification. Understanding variability ... "Evaluating risks of insertional mutagenesis by DNA transposons in gene therapy". Translational Research. 161 (4): 265-283. doi: ...
Jun dimerization protein - Wikipedia
Stewart M, Mackay N, Hanlon L, Blyth K, Scobie L, Cameron E, Neil JC (June 2007). "Insertional mutagenesis reveals progression ... Studies using high throughput viral insertional mutagenesis analysis also revealed that JDP2 functions as an oncogene. JDP2- ... July 2005). "Tumor model-specific proviral insertional mutagenesis of the Fos/Jdp2/Batf locus". Virology. 337 (2): 353-364. doi ... "Identification of oncogenes collaborating with p27Kip1 loss by insertional mutagenesis and high-throughput insertion site ...
Adeno-associated virus - Wikipedia
Rabinowitz JE, Xiao W, Samulski RJ (December 1999). "Insertional mutagenesis of AAV2 capsid and the production of recombinant ... Muralidhar S, Becerra SP, Rose JA (January 1994). "Site-directed mutagenesis of adeno-associated virus type 2 structural ... which present the threat of a random insertion and of mutagenesis, which is sometimes followed by development of a cancer. The ...
Transposable element - Wikipedia
Insertional mutagenesis uses the features of a TE to insert a sequence. In most cases, this is used to remove a DNA sequence or ... The Sleeping Beauty transposon system has been used extensively as an insertional tag for identifying cancer genes. The Tc1/ ... TEs are also a widely used tool for mutagenesis of most experimentally tractable organisms. ... Used for analysis of gene expression and protein functioning in signature-tagging mutagenesis. This analytical tool allows ...
KDM8 - Wikipedia
"Tumor suppressor gene identification using retroviral insertional mutagenesis in Blm-deficient mice". EMBO J. 25 (14): 3422-31 ...
Transfer DNA - Wikipedia
T-DNA tagging mutagenesis involves screening of populations by T-DNA insertional mutations. Collections of known T-DNA ... The same procedure of T-DNA transfer can be used to disrupt genes via insertional mutagenesis. Not only does the inserted T-DNA ... Krysan PJ, Young JC, Sussman MR (December 1999). "T-DNA as an insertional mutagen in Arabidopsis". The Plant Cell. 11 (12): ... Feldmann KA (1991-07-01). "T-DNA insertion mutagenesis in Arabidopsis: mutational spectrum". The Plant Journal. 1 (1): 71-82. ...
Transposon sequencing - Wikipedia
Because of that property, transposons have been manipulated for use in insertional mutagenesis. The development of microbial ... Transposon insertion sequencing (Tn-seq) combines transposon insertional mutagenesis with massively parallel sequencing (MPS) ... Tn-seq is useful for both the study of a single genes fitness as well as gene interactions Signature-tagged mutagenesis (STM) ... Kleckner N, Chan RK, Tye BK, Botstein D (October 1975). "Mutagenesis by insertion of a drug-resistance element carrying an ...
Monoamine oxidase A - Wikipedia
In mice, a dysfunctional MAOA gene is created through insertional mutagenesis (called Tg8). Tg8 is a transgenic mouse strain ...
Anton Berns - Wikipedia
His group explored proviral insertional mutagenesis as a means to identify new oncogenes. In 1985 he was appointed as staff ... High throughput transposon insertional mutagenesis to identify components in signaling pathways relevant for cancer, The role ...
Biomolecular engineering - Wikipedia
Youngman, PJ; Perkins, JB; Losick, R (1983). "Genetic transposition and insertional mutagenesis in Bacillus subtilis with ... One example of how mutagenesis is used is found in the coupling of site-directed mutagenesis and PCR to reduce interleukin-6 ... Site-directed mutagenesis is a technique that has been around since the 1970s. The early days of research in this field yielded ... Mutagenesis can help determine the function of proteins and the roles of specific amino acids. If an amino acid near the active ...
Antithyroid agent - Wikipedia
"Low dose ionizing radiation strongly stimulates insertional mutagenesis in a γH2AX dependent manner". PLOS Genetics. 16 (1): ...
MYH9 - Wikipedia
"Insertional mutagenesis identifies drivers of a novel oncogenic pathway in invasive lobular breast carcinoma". Nature Genetics ... Miyamoto CA, Fischman DA, Reinach FC (October 1999). "The interface between MyBP-C and myosin: site-directed mutagenesis of the ... a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. Male and ...
William Fleming Hoggan Jarrett - Wikipedia
They discovered that the virus activated cellular oncogenes by several mechanisms of insertional mutagenesis. When Jim ...
Knockout rat - Wikipedia
The SB transposon is a powerful tool for insertional mutagenesis in many vertebrate species. It recently exhibited especial ... The first published attempt had a success rate of less than 1%. Alternatively, ENU mutagenesis is a common random mutagenesis ... in vivo SB insertional mutagenesis allows multiple mutations to be quickly and easily generated in a single animal, and in a ... ENU mutagenesis involves using a chemical, N-ethyl-N-nitrosourea (ENU), to create single base changes in the genome. ENU ...
Ebola vaccine - Wikipedia
"Systems Biology-Based Investigation of Cellular Antiviral Drug Targets Identified by Gene-Trap Insertional Mutagenesis". PLOS ...