Pioglitazone Enhances Mitochondrial Biogenesis and Ribosomal Protein Biosynthesis in Skeletal Muscle in Polycystic Ovary...
... by a coordinated upregulation of genes involved in mitochondrial OXPHOS and ribosomal protein biosynthesis in muscle in PCOS. ... We hypothesized that the effect of TZDs in PCOS is, in part, mediated by changes in the transcriptional profile of muscle ... ribosomal proteins, mRNA processing reactome, translation factors, and proteasome degradation in PCOS after pioglitazone ... on gene expression in skeletal muscle of 10 obese women with PCOS metabolically characterized by a euglycemic-hyperinsulinemic ...
Colonization of the Caenorhabditis elegans gut with human enteric bacterial pathogens leads to proteostasis disruption that is...
Author summary Protein conformational diseases are one of the leading causes of geriatric death and disability, worldwide. ... They also use similar models to assess protein aggregation in muscles, neurons and gonads. Authors also show that butyrate can ... Is protein synthesis altered in these animals? Do these animals express more polyQ proteins? Does bleach definitively remove ... expressing polyQ expansion proteins in the gut, muscle and neurons, to a panel of Gram-negative enteric bacterial pathogens. ...
Inter-Individual Variability in the Adaptive Responses to Endurance and Sprint Interval Training: A Randomized Crossover Study ...
... interval training improves exercise capacity and increases mitochondrial protein content in human skeletal muscle. Open J Mol ... Skeletal muscles Is the Subject Area "Skeletal muscles" applicable to this article? Yes. No. ... increased skeletal muscle oxidative capacity [14-16,42] and resting muscle glycogen content [14-16]. Interestingly, the central ... Dissociation of Increases in PGC-1α and Its Regulators from Exercise Intensity and Muscle Activation Following Acute Exercise. ...
Down-Regulation of miR-101 in Endothelial Cells Promotes Blood Vessel Formation through Reduced Repression of EZH2 | PLOS ONE
Mir-214-dependent regulation of the polycomb protein Ezh2 in skeletal muscle and embryonic stem cells. Mol Cell 36: 61-74. * ... Histone deacetylase proteins were also described to affect angiogenesis [12], [13] and to revert EZH2-mediated gene silencing [ ... C) HUVECs protein expression analysis of EZH2 and H3K27me3 following transfection of HUVECs with pre-miR-101, EZH2 siRNA, non- ... A) HBMVECs protein expression analysis of EZH2 and H3K27me3 following transfection of HBMVECs with pre-miR-101, EZH2 siRNA, non ...
Atheroprotective Pulsatile Flow Induces Ubiquitin-Proteasome-Mediated Degradation of Programmed Cell Death 4 in Endothelial...
... decreased PDCD4 protein but not mRNA level. Immunoprecipitation revealed that pulsatile shear stress induced the coupling of ... in accordance with our previous findings that stretching human aortic smooth muscle cells in vitro altered PDCD4 protein ... The same amount of protein from each sample was incubated with cognate antibodies for 2 hr at 4°C, then with protein A/G plus ... The protein level of PDCD4 was reduced by nearly half with pulsatile shear stress as compared with static control cells (P = ...
The Effectiveness of RNAi in Caenorhabditis elegans Is Maintained during Spaceflight | PLOS ONE
Furthermore, this is the first demonstration that RNAi can be utilised to block muscle protein degradation, both on Earth and ... Finally, culture in RNAi against lysosomal cathepsins prevented degradation of the muscle-specific α-actin protein in both ... RNAi against green fluorescent protein (gfp) reduced chromosomal gfp expression in gonad tissue, which was not different from ...
Phylogenomic Analyses Support Traditional Relationships within Cnidaria | PLOS ONE
... based on mitochondrial protein-coding sequences. Mol Phylogenet Evol. 2006;41: 513-527. pmid:16876445 * View Article ... coronal muscle, and structures derived from primary tentacles of the polyp (rhopalioids/rhopalia). Maximum-likelihood analyses ... Estimation of divergence times in cnidarian evolution based on mitochondrial protein-coding genes and the fossil record. Mol ... These results are contradicted by an analysis of protein coding mitochondrial gene sequences, which recovered a paraphyletic ...
In middle-aged and old obese patients, training intervention reduces leptin level: A meta-analysis | PLOS ONE
... muscle strength OR muscle power OR muscle force OR exercise OR training) AND (overweight OR obese OR obesity). This search ... Consumption of a healthy dietary pattern results in significant reductions in C-reactive protein levels in adults: a meta- ... In this regard, studies lack standardized data such as muscle strength (preferably lower body), muscle mass or maximal oxygen ... previous observations demonstrated that training leading to bigger muscle strength and lower fatty infiltration of muscles were ...
A Novel Role for Aquaporin-5 in Enhancing Microtubule Organization and Stability | PLOS ONE
Protein concentration determination. Protein concentrations were estimated by the Bradford assay using BSA as a standard. ... 2002) Expression of aquaporin-5 and its regulation in skeletal muscle cells. Exp Mol Med 34: 69-74. * View Article ... Equivalent amounts of total protein as measured by BCA protein assay were loaded on the gels. ... The resulting soluble and pellet proteins were separated by SDS-PAGE and silver staining performed to detect proteins. ...
Chemical synthesis | PLOS ONE
Divide, conquer and reconstruct: How to solve the 3D structure of recalcitrant Micro-Exon Gene (MEG) protein from Schistosoma ... The combination of quercetin and leucine synergistically improves grip strength by attenuating muscle atrophy by multiple ... Characterization of the biochemical activity and tumor-promoting role of the dual protein methyltransferase METL-13/METTL13 in ...
PLOS Biology
Ancient bat antiviral protein blocks SARS-CoV-2. The human OAS1 protein restricts SARS-CoV-2 replication, but the horseshoe bat ... By studying motor excitability and muscle activity across the cardiac cycle of humans, Esra Al, Vadim Nikulin and colleagues ... Spyros Lytras, Sam Wilson and colleagues resurrect the functional 60-million-year-old OAS1 protein ancestral to all horseshoe ... myelin basic protein is one of the autoantigens detected by autoreactive B cells, consistent with the cortical demyelination ...
Ultrasound Microbubble Treatment Enhances Clathrin-Mediated Endocytosis and Fluid-Phase Uptake through Distinct Mechanisms |...
CME initiates by the recruitment of the proteins clathrin, the adaptor protein AP-2 and numerous other cytosolic proteins to a ... Massive palmitoylation-dependent endocytosis during reoxygenation of anoxic cardiac muscle. Elife 2: e01295. pmid:24282237 * ... Membrane proteins Is the Subject Area "Membrane proteins" applicable to this article? Yes. No. ... Recruitment of receptor proteins (termed cargo) to CCPs leads to internalization of these cargo proteins and their associated ...
Loss of the Mia40a oxidoreductase leads to hepato-pancreatic insufficiency in zebrafish | PLOS Genetics
... and has led us to identify novel phenotypes such as enlarged mitochondrial clusters in skeletal muscles. In addition, our ... caused by an imbalance in mitochondrial protein biogenesis. This mutant shares characteristics with existing reports on ...
Context-dependent reversal of odorant preference is driven by inversion of the response in a single sensory neuron type | PLOS...
Calebiro D, Koszegi Z, Lanoiselee Y, Miljus T, OBrien S. G protein-coupled receptor-G protein interactions: a single-molecule ... A total of 1 mM (−)-tetramisole hydrochloride (Sigma L9756) was added to the S-Basal buffer to paralyze body wall muscles and ... The Ga protein ODR-3 mediates olfactory and nociceptive function and controls cilium morphogenesis in C. elegans olfactory ... G protein-coupled receptor kinase function is essential for chemosensation in C. elegans. Neuron. 2004;42:581-93. pmid:15157420 ...
Semantic prioritization of novel causative genomic variants | PLOS Computational Biology
... protein-protein interactions, dominant or recessive inheritance, physico-chemical properties, sequence conservation [14], or ... A functional SNP in the regulatory region of the decay-accelerating factor gene associates with extraocular muscle pareses in ... For example, a variant (rs121908425) in the collapsin response mediator protein 1 (CRMP1) gene would be prioritized at rank 1 ... SIFT web server: predicting effects of amino acid substitutions on proteins. Nucleic Acids Research. 2012;40(W1):W452-W457. ...