Intestinal trefoil factor binds to intestinal epithelial cells and induces nitric oxide production: priming and enhancing...
Intestinal trefoil factor (ITF or TFF3), NO and epithelium-associated mucin have important roles in sustaining mucosal integrity in the gastrointestinal tract. In the present study we examined ITF-binding molecules on IEC-18 cells (an intestinal epithelial cell line) with the use of flow cytometry and localized these molecules on the cell surface by confocal microscopy. Furthermore, we studied the interaction of mucin and ITF and their co-operative effect on NO production by the epithelium. Stimulation of cells with mucin (5 mg/ml) for 90 min resulted in a 5-fold increase in ITF binding. Treatment of IEC-18 cells with actinomycin D or cycloheximide attenuated mucin-enhanced ITF binding. Ligand blot analysis confirmed the induction of ITF-binding protein in IEC-18 cells by mucin. These results indicate that transcriptional and translational mechanisms are involved in the effect of mucin. Treatment with ITF ...http://www.biochemj.org/content/338/3/745
Crosslinked Mucin Hydrogels for Drug Delivery - 2014 MRS Fall Meeting - Materials Research Society
The sustained delivery of both hydrophobic and hydrophilic drugs from hydrogels remains a challenge that requires the design of complex multifunctional synthetic polymers. In this work we exploit mucin glycoproteins, the gel-forming building blocks of natural mucus to deliver both hydrophobic and hydrophilic drugs. Mucins are a family of high molecular weight proteins which are densely glycosylated. Their central protein backbone contains hydrophobic and charged domains, while the mucin-associated glycans provide hydrogen bonding capabilities, high hydration, and negative charges. The biochemical versatility of mucins represents potential binding sites for certain drugs. If assembled into hydrogels, mucins could prevent drugs from freely diffusing out, leading to their sustained delivery. We generated methacrylated mucins which assembled into a covalently crosslinked mucin hydrogel when ...http://www.prolibraries.com/mrs/?select=session&sessionID=4641
Pathogens | Free Full-Text | Goblet Cells and Mucins: Role in Innate Defense in Enteric Infections
Goblet cells reside throughout the gastrointestinal (GI) tract and are responsible for the production and preservation of a protective mucus blanket by synthesizing and secreting high molecular weight glycoproteins known as mucins. The concept of the mucus layer functioning as a dynamic protective barrier is suggested by studies showing changes in mucins in inflammatory conditions of the GI tract, by the altered goblet cell response in germ-free animals, and by the enhanced mucus secretion seen in response to infections. The mucin-containing mucus layer coating the GI epithelium is the front line of innate host defense. Mucins are likely to be the first molecules that invading pathogens interact with at the cell surface and thus, can limit binding to other glycoproteins and neutralize the pathogen. This review will focus on what is known about goblet cell response in various GI infections and the regulatory networks that mediate goblet cell ...http://mdpi.com/2076-0817/2/1/55/xml
A call for better mucins - Biopolymers for life
Figure 1. Yearly number of publication related to mucins as reported by scicurve.com, using the keyword "mucins".. This is because mucin research spans over many fields. Biologists have been intrigued by mucins since long. But more recently, microbiologists studying our microbiome have new reasons to find this class of biopolymers particularly exciting. For instance, they realized that intestinal mucus plays key roles in the behavior of both pathogenic and commensal bacteria. This will surely lead to many more microbiology studies with mucus/mucins as a key component (1,2). Similarly, recent findings in immunology describing interactions between dendritic cells and mucin-associated glycans will also attract attention to secreted mucins in that field (3).. Some physicists and biophysicists are also fascinated by mucins. Tribologists are trying to understand and recreate ...http://biopolymersforlife.org/a-call-for-better-mucins/
The mucin-type glycoprotein podoplanin is specifically expressed by lymphatic but not | COX-2 contributes to the maintenance of...
The mucin-type glycoprotein podoplanin is specifically expressed by lymphatic but not blood vascular endothelial cells in culture and in tumor-associated lymphangiogenesis, and podoplanin deficiency results in congenital lymphedema and impaired lymphatic vascular patterning. indicated by granulosa cells in regular ovarian follicles highly, and by ovarian granulosa and dysgerminomas cell tumors. Although podoplanin was absent from regular human being epidermis mainly, its manifestation was induced in 22 of 28 squamous cell carcinomas studied strongly. These findings recommend a potential part of podoplanin in tumor development, plus they also determine the 1st commercially obtainable antibody for the precise staining of a precise lymphatic marker in archival human being tissue sections, allowing more widespread research of tumor lymphangiogenesis in human cancers thereby. Lymphatic vessels play a significant part in the maintenance of cells homeostasis1 and in the transportation of immune ...http://lifescienceexec.com/the-mucin-type-glycoprotein-podoplanin-is-specifically-expressed-by-lymphatic-but-not/
Mucin - Wikipedia
Mucins (/ˈmjuːsɪn/) are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most animals. Mucins' key characteristic is their ability to form gels; therefore they are a key component in most gel-like secretions, serving functions from lubrication to cell signalling to forming chemical barriers. They often take an inhibitory role. Some mucins are associated with controlling mineralization, including nacre formation in mollusks, calcification in echinoderms and bone formation in vertebrates. They bind to pathogens as part of the immune system. Overexpression of the mucin proteins, especially MUC1, is associated with many types of cancer. Although some mucins are membrane-bound due to the presence of a hydrophobic membrane-spanning domain that favors retention in the plasma membrane, most mucins are secreted as principal components of mucus by ...https://en.wikipedia.org/wiki/Mucin
"Comparison of structural characteristics of major membrane-bound mucin" by C. Liu
Liu, C., "Comparison of structural characteristics of major membrane-bound mucins from porcine milk and intestinal brush borders." (2003). Dairy Science Publication Database. 336 ...https://openprairie.sdstate.edu/dairy_pubdb/336/
... : (Technical Classification > Alignment Quality > High > RefOG023) - Mucins are a family of high molecular weight, heavily glycosylated proteins penetrate the membranes of epithelial cells, on the inner surface of the intestine and other organs. Mucins protect the body from infection by binding to pathogens. Mucins vary to a great extent both with regard to sequence and function (Lang et al, 2004). ROG017 is focused on gel-forming mucins. Although, all members of the family contain fast-evolving mucin domains and the combination of VWD-C8-VWC domains, the domain complexity varies among them ...http://eggnog.embl.de/orthobench/cgi/RefOGPage.py?id=RefOG023
Biotin-Linked Polyclonal Antibody to Mucin 1 (MUC1), Labelled antibody, CLOUD-CLONE CORP.(CCC)
LAA413Hu71, Biotin-Linked Polyclonal Antibody to Mucin 1 (MUC1), Homo sapiens (Human), Labelled antibody, CD227, EMA, H23AG, KL-6, PEM, PEMT, PUM, CA15-3, Cancer antigen 15-3, Carcinoma-associated mucin, Episialin, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Designed by Cloud-Clone Corp.https://www.cloud-clone.us/antibody/Biotin-Linked-Antibody-to-Mucin-1-
Cancers | Free Full-Text | Mucins and Pancreatic Cancer
Pancreatic cancer is characterized by an often dramatic outcome (five year survival | 5%) related to a late diagnosis and a lack of efficient therapy. Therefore, clinicians desperately need new biomarkers and new therapeutic tools to develop new efficient therapies. Mucins belong to an ever increasing family of O-glycoproteins. Secreted mucins are the main component of mucus protecting the epithelia whereas membrane-bound mucins are thought to play important biological roles in cell-cell and cell-matrix interactions, in cell signaling and in modulating biological properties of cancer cells. In this review, we will focus on the altered expression pattern of mucins in pancreatic cancer, from the early neoplastic lesion Pancreatic Intraepithelial Neoplasia (PanIN) to invasive pancreatic carcinomas, and the molecular mechanisms (including genetic and epigenetic regulation) and signaling pathways known to control their expression. Moreover, we ...http://www.mdpi.com/2072-6694/2/4/1794
Cancers | Free Full-Text | Mucins and Pancreatic Cancer
Pancreatic cancer is characterized by an often dramatic outcome (five year survival | 5%) related to a late diagnosis and a lack of efficient therapy. Therefore, clinicians desperately need new biomarkers and new therapeutic tools to develop new efficient therapies. Mucins belong to an ever increasing family of O-glycoproteins. Secreted mucins are the main component of mucus protecting the epithelia whereas membrane-bound mucins are thought to play important biological roles in cell-cell and cell-matrix interactions, in cell signaling and in modulating biological properties of cancer cells. In this review, we will focus on the altered expression pattern of mucins in pancreatic cancer, from the early neoplastic lesion Pancreatic Intraepithelial Neoplasia (PanIN) to invasive pancreatic carcinomas, and the molecular mechanisms (including genetic and epigenetic regulation) and signaling pathways known to control their expression. Moreover, we ...http://www.mdpi.com/2072-6694/2/4/1794/xml
Mucin producing microfollicular adenoma of the thyroid. | Journal of Clinical Pathology
An unusual case of a mucin secreting benign microfollicular adenoma of the thyroid in a 30 year old euthyroid woman is reported. Histologically, the lesion was characterised by follicular cells with the appearance of signet ring cells. Histochemistry showed the mucin content of these cells to consist uniformly of sulphated acid mucins; positive thyroglobulin immunostaining was also shown. The published work on primary mucin secreting tumours of the thyroid gland is reviewed. Dual differentiation is thought to be responsible for combined mucin secretion and hormone production in this type of neoplasm.. ...http://jcp.bmj.com/content/38/3/277
Affinity and kinetic analysis of L-selectin (CD62L) binding to glycosylation-dependent cell-adhesion molecule-1. - Oxford...
The selectin family of cell adhesion molecules mediates the tethering and rolling of leukocytes on blood vessel endothelium. It has been postulated that the molecular basis of this highly dynamic adhesion is the low affinity and rapid kinetics of selectin interactions. However, affinity and kinetic analyses of monomeric selectins binding their natural ligands have not previously been reported. Leukocyte selectin (L-selectin, CD62L) binds preferentially to O-linked carbohydrates present on a small number of mucin-like glycoproteins, such as glycosylation-dependent cell adhesion molecule-1 (GlyCAM-1), expressed in high endothelial venules. GlyCAM-1 is a soluble secreted protein which, following binding to CD62L, stimulates beta2-integrin-mediated adhesion of lymphocytes. Using surface plasmon resonance, we show that a soluble monomeric form of CD62L binds to purified immobilized GlyCAM-1 with a dissociation constant (Kd) of 108 microM. CD62L dissociates from GlyCAM-1 with a very fast ...https://www.neuroscience.ox.ac.uk/publications/15670
Human gastrointestinal tract mucins encoded by the MUC gene family - Postępy Higieny i Medycyny Doświadczalnej - Volume 54,...
Epithelial mucins, MUC gene products, are widely expressed in human organs such as airwais, the urogenital and gastrointestinal tracts, and the eyes. MUC-type mucins have very large sizes and comlex structures with very extensive O-glycosylation and are regarded as protective molecules. The aim of this review is to discuss mucin glycoproteins structure, biosynthesis and functions in normal and pathologicaly altered epithelial mucosa of human gastrointestinal tract ...http://psjd.icm.edu.pl/psjd/element/bwmeta1.element.element-from-psjc-2c2398e9-66c1-3779-953b-a261044ea531
"Human MUC1 Mucin: A Potent Glandular Morphogen" by Mark J. Hudson, Gordon W. Stamp et al.
Human MUC1 mucin is a high-molecular-weight transmembrane glycoprotein, which is apically expressed in the majority of glandular epithelia. During embryonic development, changes in the pattern of MUC1 mucin expression coincide with the onset of glandular differentiation. This mucin is also frequently overexpressed and aberrantly glycosylated in carcinomas. To investigate the potential role of MUC1 mucin in morphogenesis, a full length MUC1 cDNA was transfected into murine mammary adenocarcinoma (410.4) and Madin-Darby canine kidney (MDCK) cells. This generated four clonal cell lines. Western blotting, FACS analysis, and immunohistochemistry confirmed expression of MUC1. All four MUC1-expressing clones demonstrated altered morphogenesis when cultured in three-dimensional type I collagen gels. While parental and vector control 410.4 cells formed compact spherical structures, the MUC1-expressing clones formed complex branching structures. ...http://ecommons.aku.edu/pakistan_fhs_mc_pathol_microbiol/360/
Functional protective role for mucin glycosylated repetitive domains - UQ eSpace
Mucins carry out a number of protective roles, some of which are more easily studied than others. One mucin function is believed to be the protection of the mucosal epithelium against acidic and proteolytic damage in the stomach and intestines. In the present work, a portion of stomach mucin tandem repeat sequence (Muc6) was joined to the catalytic domain of a reporter enzyme [human milk cholesterol esterase (CE)] to determine whether the former can protect the latter protein from damage. This Muc6 domain replaced a unique series of glycosylated C-terminal repeats normally present in CE. The chimeric protein (CE/Muc6) was expressed in two different cell lines and its properties compared to recombinant full-length CE and a truncated version of CE which contained only the catalytic domain (CE/trunc). Results showed that both CE and CE/Muc6 were resistant to denaturation by acid and to proteolysis by pepsin at low pH values or by pancreatic proteases compared ...https://espace.library.uq.edu.au/view/UQ:202104
Differential mucin gene expression in human pancreatic and col...
Differential mucin gene expression in human pancreatic and colon cancer cells.: The purpose of this study was to determine the quantity and nature of the mucinshttps://www.mysciencework.com/publication/show/differential-mucin-gene-expression-in-human-pancreatic-and-colon-cancer-cells
Latest reports claim that autoantibodies directed to glycosylated mucins aberrantly, specifically - Evolving Approaches to...
Latest reports claim that autoantibodies directed to glycosylated mucins aberrantly, specifically MUC4 and MUC1, are located in individuals with colorectal cancer. Rosetta2 (Novagen), nickel purified using NiNTA agarose (Qiagen, Venlo, NL) accompanied by HPLC purification before and after O-glycosylation. Items were analyzed by MALDI-TOF and SDS-PAGE mass spectrometry on the Voyager-DE? PRO workstation (Applied Biosystems, Lifestyle Technology, Carlsbad, CA) using 2,5-dihydroxybenzoic acidity (Sigma, St. Louis, Missouri) as matrix. AB1010 Microarray MUC1, MUC4 and MUC2 glycopeptides and glycoproteins were printed on Schott Nexterion? Glide H MPX 48 (Schott AG, Mainz, Germany) as previously defined.18 In brief, triplicates of most peptides, in 150 mM sodium phosphate pH 8.5 with 0.005% CHAPS, were printed on the BioRobotics MicroGrid II spotter (Genomics Solution) having a 0.21-mm pitch using Stealth 3B Micro Spotting Pins (Telechem International ArrayIt Division). After printing, slides had ...http://www.rawveronica.com/2017/06/19/latest-reports-claim-that-autoantibodies-directed-to-glycosylated-mucins-aberrantly-specifically/
Adsorption and nanowear properties of bovine submaxillary mucin films on solid surfaces : influence of solution pH and...
The adsorption and mechanical stability of bovine submaxillary mucins (BSM) films at solid-liquid interfaces were studied with respect to both substrate hydrophobicity and solution pH. Dynamic light scattering revealed a single peak distribution in neutral aqueous solution (pH 7.4) and a small fraction with enhanced aggregation was observed in acidic solution (pH 3.8). Both substrate hydrophobicity and solution pH were found to affect the spontaneous adsorption of BSM onto solid surfaces; BSM adsorbed more onto hydrophobic surfaces than hydrophilic ones, and adsorbed more at pH 3.8 than at pH 7.4. Thus, the highest "dry" adsorbed mass was observed for hydrophobic surfaces in pH 3.8 solution. However, a highest "wet" adsorbed mass, i.e. which includes the solvent coupled to the film, was observed for hydrophobic surfaces at pH 7.4. The mechanical stability of the films was studied at the nanoscale with an atomic force microscope operated in the friction force spectroscopy mode. Results ...https://dspace.mah.se/handle/2043/17301
Identification of MUC5B, MUC5AC and small amounts of MUC2 mucins in cystic fibrosis airway secretions | Biochemical Journal
To investigate the genetic identities of the mucins secreted in cystic fibrosis (CF) airways, sputum was collected from five individuals. Samples were separated into gel and sol phases by high-speed centrifugation and the gel phase was extracted in 6 M guanidinium chloride. The 'insoluble' residue remaining after extraction of the gel phase was brought into solution by reduction/alkylation. Density-gradient centrifugation in CsCl revealed polydisperse distributions of sialic acid-containing mucins in the gel phase, insoluble residue and sol phase fractions and the degree of variation between the different individuals was low. Antibodies recognizing MUC5AC and MUC5B identified these mucins in each of the fractions. MUC2, however, was present only as a component of the insoluble residue from the gel which accounted for less than 4% by mass of the total mucins. MUC5B and MUC5AC from the gel phase were large oligomeric species composed of ...http://www.biochemj.org/content/344/2/321
Polymers | Free Full-Text | Biopolymeric Mucin and Synthetic Polymer Analogs: Their Structure, Function and Role in Biomedical...
Mucin networks are viscoelastic fibrillar aggregates formed through the complex self-association of biopolymeric glycoprotein chains. The networks form a lubricious, hydrated protective shield along epithelial regions within the human body. The critical role played by mucin networks in impacting the transport properties of biofunctional molecules (e.g., biogenic molecules, probes, nanoparticles), and its effect on bioavailability are well described in the literature. An alternate perspective is provided in this paper, presenting mucin's complex network structure, and its interdependent functional characteristics in human physiology. We highlight the recent advances that were achieved through the use of mucin in diverse areas of bioengineering applications (e.g., drug delivery, biomedical devices and tissue engineering). Mucin network formation is a highly complex process, driven by wide variety of molecular interactions, and ...http://www.mdpi.com/2073-4360/8/3/71
PARM1 (prostate androgen-regulated mucin-like protein 1)
... , Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.http://atlasgeneticsoncology.org/Genes/GC_PARM1.html
US Patent No. 6,765,127 - Invertebrate Intestinal Mucin cDNA
The invention represents the disclosure of an insect intestinal mucin (IIM) protein. The IIM protein was been identified and cloned using Trichoplusia ni larva. The cDNA and amino acid sequences have been determined and are disclosed. These sequences are useful for the production of transgenic cells, including plant cells, having insecticidal activity.. ...http://www.bpmlegal.com/6765127.html
Mucin clot test | Article about mucin clot test by The Free Dictionary
Looking for mucin clot test? Find out information about mucin clot test. see glycoprotein glycoprotein , organic compound composed of both a protein and a carbohydrate joined together in covalent chemical linkage. These... Explanation of mucin clot testhttp://encyclopedia2.thefreedictionary.com/mucin+clot+test
Role of salivary mucin in the protection of rat esophageal mucosa from acid and pepsin-induced injury.
The mucosal defensive mechanisms of the esophagus against acid and pepsin remain to be elucidated. In the present study, we investigated the contribution of the salivary mucin in maintaining the integrity of the esophageal mucosa. When an everted esohttp://www.biomedsearch.com/nih/Role-salivary-mucin-in-protection/10516145.html