Leicester Research Archive: Glomerular changes in microscopic haematuria, studied by quantitative immuno-electron microscopy...
Reproducibility studies for immuno-electron microscopy and in situ zymography demonstrated a considerable amount of variation ... Glomerular changes in microscopic haematuria, studied by quantitative immuno-electron microscopy and in situ zymography. ... The composition of glomerular basement membrane was evaluated using quantitative immuno-electron microscopy; this was ... cases were arranged into triplet groups and handled as a single batch through immuno-electron microscopy and in situ zymography ...https://lra.le.ac.uk/handle/2381/29358
Anti-Worm (Spionidae) Vasoactive Intestinal Peptide antibody for Immunoelectron Microscopy (IEM)
Vasoactive Intestinal Peptide antibody for Immunoelectron Microscopy (IEM). ... Immunoelectron Microscopy (IEM). 1 antibody 1 antibody 1 antibody 1 antibody 1 antibody 1 antibody 1 antibody 1 antibody 1 ... Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC). Alternatives ... Top referenced anti-Vasoactive Intestinal Peptide antibody for Immunoelectron Microscopy. * Bat Polyclonal Vip Primary Antibody ...https://www.antibodies-online.com/hormone-activity-pathway-42/vip-antibody-5523/worm-iem-134895/
Immunoelectron Microscopy on Receptor and Ligand Sorting Sites | Springer for Research & Development
Geuze H.J. (1986) Immunoelectron Microscopy on Receptor and Ligand Sorting Sites. In: Greten H., Windler E., Beisiegel U. (eds ... The increasing need for precise subcellular detection has coincided with recent developments of powerful immunoelectron ...https://rd.springer.com/chapter/10.1007/978-3-642-70956-2_24
Principles of immunoelectron microscopy - Immune Response
Immunoelectron microscopy can be defined as any technique that uses antibodies, or molecules that interact with antibodies for ... Immunoelectron microscopy can be defined as any technique that uses antibodies, or molecules that interact with antibodies (for ... Principles of immunoelectron microscopy. Last Updated on Wed, 06 Jun 2018 , Immune Response ... Samples for scanning electron microscopy must be fixed and dehydrated, as in transmission electron microscopy, but instead of ...https://www.barnardhealth.us/immune-response/principles-of-immunoelectron-microscopy.html
Immunoelectron microscopy reveals colocalization of myo | Open-i
Immunoelectron microscopy reveals colocalization of myopalladin and CARP in mouse cardiac myofibrils. (A-C) Isolated myofibrils ... Figure 8: Immunoelectron microscopy reveals colocalization of myopalladin and CARP in mouse cardiac myofibrils. (A-C) Isolated ... Figure 8: Immunoelectron microscopy reveals colocalization of myopalladin and CARP in mouse cardiac myofibrils. (A-C) Isolated ... Immunofluorescence and immunoelectron microscopy studies revealed that myopalladin also colocalized with CARP in the central I- ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2169455_JCB0012108.f8&req=4
Immunoelectron microscopy analysis of HCMV gpUL73 (gN) localization, Archives of Virology | 10.1007/s00705-002-0810-x | DeepDyve
"Immunoelectron microscopy analysis of HCMV gpUL73 (gN) localization, Archives of Virology" on DeepDyve, the largest online ... Immunoelectron microscopy analysis of HCMV gpUL73 (gN) localization. Immunoelectron microscopy analysis of HCMV gpUL73 (gN) ... Immunoelectron microscopy analysis of HCMV gpUL73 (gN) localization. Pignatelli, S.; Dal Monte, P.; Zini, N.; Valmori, A.; ... lp/springer_journal/immunoelectron-microscopy-analysis-of-hcmv-gpul73-gn-localization-ebuRRyMK2w ...https://www.deepdyve.com/lp/springer_journal/immunoelectron-microscopy-analysis-of-hcmv-gpul73-gn-localization-ebuRRyMK2w
Introduction to Immunohistochemistry - Immuno-Electron Microscopy
Main Page , Immuno-Electron Microscopy. Electron microscopic (EM) immunohistichemical techniques can be divided into two groups ... These methods were originally introduced for electron microscopy (Faulk and Taylor 1971) as the gold particles are easily ... visible under the electron microscope, but they are also useful for light microscopy. ...http://www.ihcworld.com/_intro/immunoem.htm
Microscopy, Immunoelectron | Anatomical Neuropharmacology Unit
This is an historical archive of the activities of the MRC Anatomical Neuropharmacology Unit (MRC ANU) that operated at the University of Oxford from 1985 until March 2015. The MRC ANU established a reputation for world-leading research on the brain, for training new generations of scientists, and for engaging the general public in neuroscience. The successes of the MRC ANU are now built upon at the MRC Brain Network Dynamics Unit at the University of Oxford.. ...http://mrcanu.pharm.ox.ac.uk/research-and-techniques/microscopy-immunoelectron
Localization of subunits in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy :: MPG.PuRe
Localization of subunits in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy ... Microscopy, Electron; Molecular Structure; Molecular Weight; Multienzyme Complexes/im [Immunology]; *Multienzyme Complexes/ul [ ... The subunit topography of the Thermoplasma acidophilum proteasome was determined by immunoelectron microscopy using ... in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy. FEBS Letters., 290(1-2), 186-190. ...http://pubman.mpdl.mpg.de/pubman/faces/viewItemOverviewPage.jsp?itemId=escidoc:1582775
Immunoelectron microscopy identifying avidin (8-nm gol | Open-i
Immunoelectron microscopy identifying avidin (8-nm gold) and cathepsin L (15-nm gold) immunoreactivity in organelles from late ... Figure 4: Immunoelectron microscopy identifying avidin (8-nm gold) and cathepsin L (15-nm gold) immunoreactivity in organelles ... Figure 4: Immunoelectron microscopy identifying avidin (8-nm gold) and cathepsin L (15-nm gold) immunoreactivity in organelles ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2140175_JCB15003.f4&req=4
A universal vaccine for serogroup B meningococcus | PNAS
Immunoelectron Microscopy.. Whole-cell, negative-stain, ImmunoGold labeling was performed by using a modified procedure ... ImmunoGold labeling and transmission electron microscopy. Analysis of BZ83 strain was performed with antisera raised against ... Anna Rita Taddei for technical help with ImmunoGold labeling and transmission electron microscopy; Maria Scarselli for help in ...https://www.pnas.org/content/103/29/10834.long
Immunoelectron microscopy analysis. To confirm ultrastructurally the presence of synapses on newborn GCs in the adult OB, ... Double-labeling immunoelectron microscopy of newborn GCs in the EPL. Newborn eGFP-positive GCs make and receive GABAergic ... E-G , Double immunoelectron microscopy for gephyrin (silver grains, arrows) and eGFP (*) in GAD67-GFP mice, depicting eGFP- ... To confirm these observations ultrastructurally, we performed dual-labeling immunoelectron microscopy in OB sections of GAD67- ...http://www.jneurosci.org/content/29/48/15039
Abnormal accumulation of autophagic vesicles correlates with axonal and synaptic pathology in young Alzheimer's mice...
Immunoelectron microscopy. Sections of 50 μm from 4.5-month-old PS1/APP mice were first washed with PBS and incubated in a 50 ... Immunoelectron microscopy detection of Aβ was much less stronger than of APP, since optimal intracellular labeling with the ... To that end, we performed immunoelectron microscopy for APP and Aβ42 in 4.5-month-old PS1/APP hippocampus. Silver-enhanced ... Nixon RA, Wegiel J, Kumar A et al (2005) Extensive involvement of autophagy in Alzheimer disease: an immuno-electron microscopy ...https://link.springer.com/article/10.1007%2Fs00401-011-0896-x
NEURONAL SPECIFIC TARGETING OF CAVEOLIN EXPRESSION TO RESTORE SYNAPTIC SIGNALING AND IMPROVE COGNITIVE FUNCTION IN THE...
Routine and Immunoelectron Microscopy. Brains were transcardially perfusion fixed with standard Karnovsky's fix, 4% ... 4A). Immunofluorescence microscopy demonstrated that young Cav-1 KO mice had increased Aβ staining in Niss1 positive neurons in ... Routine electron microscopy (EM) revealed a significant reduction in hippocampal synapses (i.e., post synaptic densities) in ... Immunofluorescence Confocal Microscopy. Tissue and cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS ...http://www.freepatentsonline.com/y2013/0224163.html
High-pressure freezing immunoelectron microscopy. BON cells were cultured on thermanox coverslips (Nunc) and fitted in a 6 mm ... TIRF microscopy does not allow imaging all the SGs present at the cell cortex but only those which are localized in the ... The exchange rates between the three states were derived from the analysis of SG dynamics by TIRF microscopy (Table 1). To ... 7D). Finally, using the fact that, by TIRF microscopy, changes in SG fluorescence intensity report changes in the position ...http://www.jneurosci.org/content/32/7/2564
Rab27a | JCB
Immunoelectron Microscopy. Immunoelectron microscopy was performed basically as described previously (Buscà et al. 1996). ... Using immunofluorescence and immunoelectron microscopy studies, we show that in normal melanocytes Rab27a colocalizes with ... A) Phase-contrast microscopy images of a normal (a) and GS (b) melanocyte. (B) Confocal immunofluorescence microscopy images of ... C) Electron microscopy images of a dendritic extension of a normal (e) and GS (f) melanocyte. Note that despite the reduction ...http://jcb.rupress.org/content/152/4/843
Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation | JCB
Immunoelectron microscopy. Cells were fixed with 0.5% glutaraldehyde and 3% paraformaldehyde in 100 mM phosphate buffer, pH 7.2 ... We investigated the precise localization of Bqt3 and -4 proteins using immunoelectron microscopy. As shown in Fig. 3 A, when ... as determined by immunoelectron microscopy. Arrowheads indicate GFP-Bqt4 or -Bqt3, in which GFP was fused to the N terminus of ... Fluorescence microscopy in three dimensions. Methods Cell Biol. 30:353-377. doi:10.1016/S0091-679X(08)60986-3. ...http://jcb.rupress.org/content/187/3/413/
Electron microscopy (EM) core facility | Biocenter Oulu
Biocenter Oulu Electron Microscopy Core Facility provides services and training in various electron microscopy techniques for ... Immunoelectron Microscopy (IEM). For the localization of molecules at the ultrastructural level we apply Tokuyasu ... Biocenter Oulu Electron Microscopy Core Facility provides services and training in various electron microscopy techniques for ... SEM Applications / Focused Ion Beam - Scanning Electron Microscopy (FIB-SEM). With this method it is possible to obtain series ...http://www.oulu.fi/biocenter/em
The central element of the synaptonemal complex in mice is organized as a bilayered junction structure | Journal of Cell Science
melanogaster using immunoelectron microscopy and structured illumination microscopy have suggested that central region proteins ... Immunoelectron microscopy analysis of the location of the N-terminal region of SYCP1 and SYCE1 in a frontal view of the ... Immunoelectron microscopy localization of the N-terminal region of SYCP1 in a lateral view of the central element. (A) ... Immunoelectron microscopy localization of the central element proteins in a lateral view of the central element. (A) Schematic ...http://jcs.biologists.org/content/129/11/2239
Targeting to C-Terminal Myosin Heavy Chain May Explain Mechanotransduction Involving Focal Adhesion Kinase in Cardiac Myocytes ...
Immunoelectron Microscopy. Fragments of left ventricle were fixed in paraformaldehyde/glutaraldehyde (Electron Microscopy ... confocal microscopy of double-labeled isolated cardiac myocytes and immunoelectron microscopy with anti-Fak antibody. Fak ... Indeed, the immunoelectron microscopy imaging of cardiac myocytes from left ventricles of control rats showed that anti-Fak ... Fak subcellular localization was further examined by immunoelectron microscopy. As shown in the representative examples of ...http://circres.ahajournals.org/content/96/1/73
Specific domains drive VM32E protein distribution and integration in Drosophila eggshell layers | Journal of Cell Science
C) Immunoelectron microscopy of VM32E and VM32E-MYC proteins by double immunogold staining in stage 10B VM32E-MYC egg chamber. ... Immunoelectron microscopy. Ovaries were removed in cold insect Ringer's solution and fixed as described (Pascucci et al., 1996 ... As control of the quality of our immunoelectron microscopy procedure, we checked the proper distribution of the VM26A.2 protein ... We thank L. Alibardi for helpful suggestions on immunoelectron microscopy, M. Rogers for helping to prepare the manuscript, S. ...http://jcs.biologists.org/content/114/15/2819
Renal Production, Uptake, and Handling of Circulating αKlotho | American Society of Nephrology
Immunoelectron Microscopy. Mouse recombinant Klotho protein (0.1 mg/kg BW) was intraperitoneally injected once into kl/kl mice ... Rat monoclonal anti-human Klotho antibody, KM20761,2 was used for immunoblotting and immunoelectron microscopy; and the ... immunoelectron microscopy (EM) did not detect endogenous αKlotho protein in the kidney of kl/kl mice (data not shown). ... which is consistent with immunoelectron micrographs (Figure 4C) showing no visible αKlotho antigen in Bowman's space. 125I-α ...https://jasn.asnjournals.org/content/27/1/79
Translocation of molecular chaperones to the titin springs is common in skeletal myopathy patients and affects sarcomere...
Immunoelectron microscopy using anti-HSP27 antibodies showed massive labelling of the I-band on either side of the Z-disc, ... Immunoelectron microscopy. 4% PFA fixed biopsy samples were cut into longitudinal 50-μm-thick sections using a VT 1000S Leica ... Correlative immunofluorescence and immunoelectron microscopy showed that two small HSPs (HSP27 and αB-crystallin) and the ATP- ... Correlative immunofluorescence and immunoelectron microscopy to localize HSP27, αB-crystallin and HSP90 in normal and diseased ...http://pubmedcentralcanada.ca/pmcc/articles/PMC5603016/
The cytoskeletal protein septin 11 is associated with human obesity and is involved in adipocyte lipid storage and metabolism |...
Immunoelectron microscopy. Ultrathin sections of 3T3-L1 adipocytes were incubated with anti-SEPT11 and 10 nm gold conjugated ... 3a). Immunoelectron microscopy of 3T3-L1 adipocytes showed SEPT11 immunolabelling associated with plasma membrane invaginations ... and our immunoelectron microscopy data, demonstrate that this protein associates with adipocyte caveolae. Further support for ... Moreover, triple-labelling microscopy studies showed that SEPT11 co-localised with CAV1 and GFP-FABP5 to LDs in oleate-treated ...https://link.springer.com/article/10.1007/s00125-016-4155-5
A Plant-Specific Dynamin-Related Protein Forms a Ring at the Chloroplast Division Site | Plant Cell
Immunofluorescence and Immunoelectron Microscopy. Immunofluorescence microscopy was performed according to a protocol described ... Immunoelectron microscopy was performed as described previously (Itoh et al., 1999). In brief, cells were fixed by rapid ... Under immunoelectron microscopy, the gold particles showing the localization of CmDnm2 did not overlay the outer PD ring, ... Because of the technical limitations of immunoelectron microscopy, such as antigen accessibility, this observation may be ...http://www.plantcell.org/content/15/3/655?ijkey=329a5a9a3714a2cb868a301c2ba35772828fb72a&keytype2=tf_ipsecsha