Ligand-Gated Ion Channel Subunit Partnerships: GABAAReceptor α6 Subunit Gene Inactivation Inhibits δ Subunit Expression |...
Homozygous mutant mice had no overt defects and could breed normally (see Behavioral Characterization of α6 −/− Mice). By the ... was generated from a 6 kb mouse 129 strain α6 subunit gene fragment (Jones et al., 1996), comprising part of exon 4 through to ... mutant mice showed as much exploratory activity as individuals with normal levels of α6 and δ proteins (Table 2). Mutant as ... mutant mice seem indistinguishable from wild-type littermates. The adult α6 null/δ-deficient mice are active and agile, whether ...
Abnormalities in Neuronal Process Extension, Hippocampal Development, and the Ventricular System of L1 Knockout Mice | Journal...
The mean body weights of mutant and wild-type mice were similar (Table 1). In most of the mutant mice, callosal axons appeared ... 1997) Retarded formation of the hippocampal commissure in embryos from mouse strains lacking a corpus callosum. Hippocampus 7:2 ... Genotypes of mutant mice were determined by PCR analysis. Genomic DNA was isolated from tail segments of mice as described ( ... Enlarged ventricles and septal defects in L1 mutant mice. Serial frontal sections of the brains of adult L1-minus male mice ...
Glutamatergic Neurons of the Mouse Medial Septum and Diagonal Band of Broca Synaptically Drive Hippocampal Pyramidal Cells:...
Experiments were performed using VGLUT2-eGFP transgenic mice unless otherwise stated. VGLUT2-eGFP mice [strain name: STOCK Tg( ... were obtained from the Mutant Mouse Regional Resource Center (MMRRC). In these mice, the genotype was modified to contain ... strain name: STOCK Tg(Gad2-EGFP)31Gsat/Mmcd]. For both transgenic mouse strains, the background was FVB/N-Swiss Webster hybrid ... Brain slices containing the MS-DBB were obtained from 12- to 18-d-old VGLUT2-eGFP or GAD65-eGFP transgenic mice. Mice were ...
Kisspeptin-GPR54 Signaling Is Essential for Preovulatory Gonadotropin-Releasing Hormone Neuron Activation and the Luteinizing...
2007) reported that female mice from a new Gpr54 mutant mouse line could mount an LH surge. In direct contradiction to that ... P protocol with mice being killed between 5:45 and 6:15 P.M., the time of peak LH secretion in the 129S6/Sv/Ev strain. The ... whereas none of the five Gpr54-null or five Kiss1-mutant mice exhibited LH surges (p , 0.01; individual values for all 10 mice ... of 129S6/Sv/Ev mice. In this protocol, mice were treated in exactly the same manner as for the OVX+E mice with the exception ...
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... mutant mice compared with their wild-type littermates (Fig.4). In wild-type mice, the primary dendrites could be seen as ... The second strain ofFgf2 knock-out mice was created in a 129Sv:C57BL /6 background (Ortega et al., 1998). Animals used for the ... was increased in the mutants. In 3- to 4-month-old male mice, sleeping time was 159 ± 9.4 min in wild-type mice and 218 ± 7 min ... Fgf2 null mutant mice show a significant decrease in the SMI-32-stained pyramidal cells in both the infragranular and ...
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... cav1.3 was an excellent candidate for gem because Cav1.3 knock-out mice, similarly to gem mutant zebrafish, exhibit deafness ( ... Zebrafish strains. The tc123d mutation was initially isolated in an N-ethyl-N-nitrosourea (ENU) large-scale mutagenesis screen ... Secondary Abs were Alexa Fluor (488, 546, 568) conjugated goat anti-rabbit IgG, goat anti-mouse IgG, and goat anti-mouse IgM ... 12 mutants; n = 6 wild type), 59 ± 20% in tc123d at 5 dpf (n = 7 mutants; n = 4 wild type), and 36 ± 16% in tn004 at 5 dpf (n ...
Phosphorylation of Homer3 by Calcium/Calmodulin-Dependent Kinase II Regulates a Coupling State of Its Target Molecules in...
... was dramatically reduced in P/Q-Ca2+-channel knock-out mice at the P14 stage but not in the mutant mice that was deficient in ... Phosphorylation-induced retarded migration of Homer3 was observed in Coomassie brilliant blue (CBB)-strained gel. ... Both mutants, in which Ser120 substituted to Ala a S120A mutant and a S159A mutant, showed reduced phosphorylation by CaMKII ( ... 1994) Deficient cerebellar long-term depression and impaired motor learning in mGluR1 mutant mice. Cell 79:377-388. ...
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BAC transgenic mice from Mutant Mouse Regional Resource Center (www.gensat.org) (Gong et al., 2003, 2007). Frizzled3fl/fl mice ... wt STR versus wt GP, n = 18; wt STR versus ko GP, n = 29; ko STR versus ko GP, n = 11; ko STR versus wt GP, n = 28. *p , 0.05 ( ... D2-Cre;Rosa26-EYFP and Isl1-Cre;Rosa26-EYFP mice show specific labeling of the STR (B, E). Nkx2-1-Cre;Rosa26-EYFP mice show ... 3 mice per genotype; p , 0.01). Similar to Fzd3−/− mice, many DARPP32+ axons in Wnt5a−/− mice were no longer confined to their ...
In Vivo Imaging of Intersynaptic Vesicle Exchange Using VGLUT1Venus Knock-In Mice | Journal of Neuroscience
1H). Thus, in the mutant mice we generated, VGLUT1Venus replaces VGLUT1 without upregulation or downregulation artifacts. ... which had been isolated from a λFIXII genomic library of the SV129 mouse strain (Stratagene). In the targeting vector, the STOP ... Validity and assets of the VGLUT1Venus KI mouse model. The generation of the VGLUT1Venus KI mouse model involved tagging of an ... 1D). Except for initial comparative analyses of VGLUT1VenusNeo and VGLUT1Venus mice (Fig. 1), Cre recombined VGLUT1Venus mice ...
RIM Promotes Calcium Channel Accumulation at Active Zones of the Drosophila Neuromuscular Junction | Journal of Neuroscience
This finding is also consistent with decreases in RRP size observed in conditional knock-out RIM mutant mice and indicates that ... We also thank Grae Davis for discussion of unpublished data, Stephan Sigrist for gifts of fly strains, and the Bloomington and ... rab3rup mutant, and rab3rup-RIMEx73 double mutant larvae. Brp distribution is similar between rab3rup single mutants and rab3 ... Boxed regions of the RIMEx73 and RIMEx98 mutant genes denote the excised region of each mutant. In the RIMEx73 mutant, the gray ...
Compensation of Inositol 1,4,5-Trisphosphate Receptor Function by Altering Sarco-Endoplasmic Reticulum Calcium ATPase Activity...
... was introduced to generate a strain of the genotype dgq221C/plc21CP319; itprwc703/wc361. Viability in this triple mutant strain ... Ginzburg L, Futerman AH (2005) Defective calcium homeostasis in the cerebellum in a mouse model of Niemann-Pick A disease. J ... None of the PLCβ mutant strains has flight defects by itself (Fig. 4B) (data not shown). Furthermore, the two mutant alleles ... Drosophila strains.. Different mutant alleles for the itpr gene were tested for flight. The viable heteroallelic combinations ...
Mice Lacking Sodium Channel β1 Subunits Display Defects in Neuronal Excitability, Sodium Channel Expression, and Nodal...
... and Kv1.2 appeared to be unchanged in the mutant mice compared with control. A, β1 (+/+) mice: β1 subunits (red), caspr 1 ( ... The time course of inward currents at -30 mV or outward currents at +20 mV were similar in the two mouse strains. Finally, the ... A, β1(-/-) mice: pan-sodium channels (green), caspr 1 (red). B, β1(-/-) mice: caspr 1 (green), Kv1.1 (red). C, β1(-/-) mice: Na ... Similar to β1(-/-) mice, contactin (-/-) mice are ataxic and die by P18 (Berglund et al., 1999). Contactin (-/-) mice exhibited ...
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Two mouse strains were primarily used for experiments: Ilkfl/fl;Plp-CreERT/+ mice (Ilk cKO) and Ilkfl/fl;+/+ [wild-type (WT)] ... we used a conditional knock-out approach taking advantage of the Ilkfl/fl mutant mouse (Terpstra et al., 2003) (Fig. 1B). To ... Ilkfl/fl mice were crossed to Plp-CreERT mice to generate Ilkfl/fl;Plp-CreERT conditional knock-out mice (hereafter referred to ... A, Ilk cKO mice were generated by crossing Plp-CreERT transgenic mice with Ilkfl/fl mice. Tamoxifen was administered to induce ...
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While the age of puberty varies widely in different mouse strains (Pinter et al., 2007), the examination of littermates in ... 2009) Kisspeptin/Gpr54-independent gonadotrophin-releasing hormone activity in Kiss1 and Gpr54 mutant mice. J Neuroendocrinol ... A, Female mice were mated with wild-type male mice for 90 d. Each line represents an individual female mouse. The black dot ... mouse. A CNS STAT5 KO mouse has also been produced (Lee et al., 2008), and the mice are fertile although specific parameters of ...
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We used mutant mice and their corresponding wild-type littermates in which Cre recombinase expression was driven by the D1R ... A, Representative images delineating the connectivity from the striatum to output nuclei in D1R-Cre and D2R-Cre mice. Str, ... 7 mice) and only a negligible recombination in D2R-Cre mice (,3% of ChaT-positive cells infected; n = 7 mice). Next, a CAG-DIO- ... 2008) CEP-1347 reduces mutant huntingtin-associated neurotoxicity and restores BDNF levels in R6/2 mice. Mol Cell Neurosci 39:8 ...
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1996) Defective neuromuscular synaptogenesis in agrin-deficient mutant mice. Cell 85:525-535. ... GST and GST-rapsyn were expressed in the BL21 strain. Fusion proteins were extracted in Sarkosyl and isolated on glutathione ... Sol8 and C2 mouse muscle cells were maintained in growth medium consisting of DMEM-HI, supplemented with 20% fetal bovine serum ... Immortalized muscle cell lines generated from wild type and AChR-β3F/3F mice carrying the H-2Kb-tsA58 transgene were grown as ...
Novel Hippocampal Interneuronal Subtypes Identified Using Transgenic Mice That Express Green Fluorescent Protein in GABAergic...
Pronuclear microinjections of transgene DNA into one-cell stage embryos of the inbred albino mouse strain FVB/N were performed ... 1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38. ... Founder mice were bred to wild-type FVB mice (Harlan Sprague Dawley, Indianapolis, IN), and resultant F1 heterozygotic mice ... Screening of transgenic mice. Transgenic mice were screened via PCR similar to the method described by Busler and Li (1996). ...
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In vertebrates, chick MNR2 and mouse HB9 are expressed in presumptive somatic motoneuron progenitors. Mouse HB9 maintains ... lim3 mutants have pathfinding defects in which SNb motoneurons are diverted into the SNd nerve and terminate outside their ... Transgenic UAS-HB9 fly strains were generated using standard P element transformation methods. For each transgene, multiple ... mouse anti-Tau, 1:40; mouse anti-Eve, 1:20 (C. Goodman, University of California Berkeley, Berkeley, CA); rat anti-reversed ...
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2012) Reduced excitatory neurotransmission and mild autism-relevant phenotypes in adolescent Shank3 null mutant mice. J ... 2014) Absence of strong strain effects in behavioral analyses of Shank3-deficient mice. Dis Model Mech 7:667-681. https://doi. ... Shank3a-deficient 3xTg-AD mice were generated by crossing homozygous 3xTg-AD mice with homozygous B6(Cg)-Shank3tm1.2Bux/J mice ... mice compared with WT mice, and a complete KO in homozygous (Ho/KO) mice, but no changes in isoforms Shank3c, Shank3d, and ...
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The expression plasmid was transformed into a BL21(DE3)pLysS Escherichia coli strain. After induction with 1 mmisopropyl-1-thio ... 1999) Postnatal distribution of cpp32/caspase 3 mRNA in the mouse central nervous system: an in situ hybridization study. J ... In transfection studies using dominant negative mutant constructs of caspase-8 and -9, we have demonstrated that etoposide- ... 1996) Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice. Nature 384:368-372. ...
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... wild-type and mutant egl-2) or 10 ng (wild-type and mutantmeag) cRNA. Wild-type and mutant EGL-2 currents were obtained in ... A full-length mouse ether-a-go-go(meag) clone (kindly provided by Barry Ganetzky) was cloned into the pMXT oocyte expression ... This work was supported by Public Health Service Grants R01NS30187 (J.T.) and RO1NS24785 (L.S.). Many nematode strains were ... For mutant egl-2 alleles, PCR products from theegl-2 gene using mutant and wild-type genomic DNA as template were gel-purified ...
Sleep and Brain Energy Levels: ATP Changes during Sleep | Journal of Neuroscience
2006) Glycogen content in the cerebral cortex increases with sleep loss in C57BL/6J mice. Neurosci Lett 402:176-179. ... 2006) Mitochondrial dysfunction in Drosophila PINK1 mutants is complemented by parkin. Nature 441:1157-1161. ... and that the changes are dependent on the age and strain of the animals (Gip et al., 2002). Because of these variable results, ...
Exogenous Brain-Derived Neurotrophic Factor Rescues Synaptic Dysfunction in Mecp2-Null Mice | Journal of Neuroscience
... in wild-type mice and 156.3 ± 15.6 pA (n = 26) in null mice, and EPSC amplitude remained elevated in the mutants throughout the ... patients exhibit a significant increase in mean respiratory frequency that is recapitulated in the strain of Mecp2-null mice ... 2006) The disease progression of Mecp2 mutant mice is affected by the level of BDNF expression. Neuron 49:341-348. ... 1999) Brain-derived neurotrophic factor levels in the nervous system of wild-type and neurotrophin gene mutant mice. J ...
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Jeffrey D. Rothsteins laboratory), SOX9-EGFP mice (University of California Davis MMRRC, strain 011019-UCD), PDGFRA-EGFP mice ... spinal cord glutamate transport capacity and reduced sensitivity to riluzole in a transgenic superoxide dismutase mutant rat ... Mice.. Wild-type mice (C57BL/6J; The Jackson Laboratory), SOD1 G93A mice (The Jackson Laboratory, stock #004435), BAC-GLT1-EGFP ... In this mouse strain, OPCs have EGFP signaling only in the nuclei, which makes it easy to visualize colocalization with SOX9, ...
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... recombinant mouse LCN2 protein was expressed as a GST fusion protein in the BL21 strain of E. coli, which does not synthesize ... 2003) Switch-of-function mutants based on morphology classification of Ras superfamily small GTPases. Cell 113:315-328. ... mouse monoclonal anti-GFAP antibody (Biogenex); monoclonal anti-α-tubulin clone B-5-1-2 mouse ascites fluid (Sigma-Aldrich)] ... goat polyclonal anti-mouse LCN2 antibody (R&D Systems); rabbit polyclonal anti-mouse LCN2/NGAL antibody (Santa Cruz ...
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We show that when maintained under standard diet, mutant mice display a lean phenotype accompanied by increased leptin ... 1999) Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet 21:70-71. ... C, D, Western blot analyses of Socs3 and β-actin in MBH lysates of WT and Socs3ΔMBH mice. Mice were fasted overnight and ... We generated two independent cohorts of WT and Socs3ΔMBH mice, all injected with Ad-Cre at 10-14 weeks of age. Mice were fed ad ...