Impaired apoptosis, extended duration of immune responses, and a lupus-like autoimmune disease in IEX-1-transgenic mice
Mice, Inbred C57BL * Mice, Inbred DBA * Mice, Transgenic * Neoplasm Proteins* * Splenomegaly / genetics ... Impaired apoptosis, extended duration of immune responses, and a lupus-like autoimmune disease in IEX-1-transgenic mice Proc ...
Dynamic I-V curves are reliable predictors of naturalistic pyramidal-neuron voltage traces
Mice, Inbred C57BL * Models, Neurological* * Nonlinear Dynamics* * Patch-Clamp Techniques / methods * Pyramidal Cells / ...
Overexpression of CD97 in intestinal epithelial cells of transgenic mice attenuates colitis by strengthening adherens junctions
To investigate the function of CD97 in colorectal carcinogenesis, transgenic Tg(villin-CD97) mice overexpressing CD97 in ... Mice, Inbred C57BL * Mice, Transgenic * Microscopy, Electron * Proto-Oncogene Proteins c-akt / metabolism ... mice. Transepithelial resistance was not altered in Tg and Ko mice, indicating that tight junctions were not affected. In Tg ... To investigate the function of CD97 in colorectal carcinogenesis, transgenic Tg(villin-CD97) mice overexpressing CD97 in ...
Elucidation of the distal convoluted tubule transcriptome identifies new candidate genes involved in renal Mg(2+) handling
Mice, Inbred C57BL * Mice, Transgenic * Nephrons / metabolism * Transcriptome* / genetics Substances * Carrier Proteins ... Indeed, all four genes show significant upregulation in the DCT of mice fed a Mg(2+)-deficient diet. By elucidating the Mg(2+)- ... To this end, transgenic mice expressing enhanced green fluorescent protein under a DCT-specific parvalbumin promoter were ...
Adenovirus-mediated delivery of relaxin reverses cardiac fibrosis
We have evaluated the effectiveness of systemic adenovirally delivered mouse relaxin on reversing fibrosis in a transgenic ... Mice, Inbred C57BL * Mice, Transgenic * Rats * Receptors, Adrenergic, beta-2 / genetics * Receptors, Adrenergic, beta-2 / ... Ad-mRLN, but not Ad-GFP, treatment reversed the increased left ventricular collagen content in beta(2)AR mice to control levels ... Recombinant adenoviruses expressing green fluorescent protein (Ad-GFP), rat relaxin (Ad-rRLN) and mouse relaxin (Ad-mRLN) were ...
Anatomically heterogeneous populations of CB1 cannabinoid receptor-expressing interneurons in the CA3 region of the hippocampus...
Mice, Inbred C57BL * Mice, Transgenic * Neural Inhibition / physiology * Neuronal Plasticity / physiology * Patch-Clamp ...
Expression of a Mutant SEMA3A Protein with Diminished Signalling Capacity Does Not Alter ALS-Related Motor Decline, or Confer...
Mice, Inbred C57BL * Mice, Mutant Strains * Mice, Transgenic * Motor Neurons / drug effects ... the G93A-hSOD1 ALS mouse line) and an injury model (BotoxA-induced paralysis). ALS mice that either expressed 1 or 2 mutant ... Using a mouse model expressing a mutant SEMA3A with diminished signaling capacity, we studied the influence of SEMA3A signaling ... We also investigated the effects of BotoxA-induced paralysis on the sprouting capacity of NMJs in the K108N-SEMA3A mutant mouse ...
CDK2 inhibitors as candidate therapeutics for cisplatin- and noise-induced hearing loss
Mice, Inbred C57BL * Mice, Knockout * Mitochondria / metabolism * Protein Kinase Inhibitors / therapeutic use* ... protected adult mice and rats against cisplatin- and noise-induced hearing loss. CDK2-deficient mice displayed enhanced ... 4,385 small molecules in a cochlear cell line and identified 10 compounds that protected against cisplatin toxicity in mouse ...
In vivo tolerization of Th1 lymphocytes following a single feeding with ovalbumin: anergy in the absence of suppression
Mice, Inbred C3H * Mice, Inbred C57BL * Ovalbumin / administration & dosage * Ovalbumin / immunology* * Serum Albumin / ... for the existence of a state of anergy in mice orally fed with ovalbumin (OVA). In addition, it has been shown that peripheral ... Reversal of the tolerant state in vivo was established by antibody production in irradiated mice adoptively transferred with ...
Resveratrol Attenuates Trimethylamine-N-Oxide (TMAO)-Induced Atherosclerosis by Regulating TMAO Synthesis and Bile Acid...
Mice, Inbred C57BL * Resveratrol * Stilbenes / administration & dosage* Substances * Bile Acids and Salts ... deconjugation and fecal excretion in C57BL/6J and ApoE(-/-) mice. This was associated with a decrease in ileal BA content, ... In mice treated with antibiotics, RSV neither decreased TMAO levels nor increased hepatic BA synthesis. Additionally, RSV- ... We found that RSV attenuated trimethylamine-N-oxide (TMAO)-induced AS in ApoE(-/-) mice. Meanwhile, RSV decreased TMAO levels ...
RIPK3 Activation Leads to Cytokine Synthesis that Continues after Loss of Cell Membrane Integrity
Mice, Inbred C57BL * Necroptosis* * RNA, Messenger / genetics * RNA, Messenger / metabolism * Receptor-Interacting Protein ...
Anti-angiogenic role of angiostatin during corneal wound healing
... and anti-mouse plasmin B chain antibodies. Mouse co … ... Mice, Inbred C57BL * Photorefractive Keratectomy / adverse ... We generated polyclonal rabbit anti-mouse angiostatin antibodies directed against each of the five kringle domains, (K1-5) ... Purified plasminogen was incubated with lysate of mouse corneal epithelial cells from wild type mice in the presence or absence ... and anti-mouse plasmin B chain antibodies. Mouse corneas were immunostained with anti-K1 angiostatin antibody after excimer ...
Histone H3 lysine 9 di-methylation as an epigenetic signature of the interferon response
Mice * Mice, Inbred C57BL * Virus Diseases / immunology* Substances * Histones * Interferons * Histone-Lysine N- ...
Gut Microbial Metabolite TMAO Enhances Platelet Hyperreactivity and Thrombosis Risk
Mice, Inbred C57BL * Thrombosis / metabolism* * Thrombosis / pathology Substances * Chlorides * Ferric Compounds * Methylamines ... Animal model studies employing dietary choline or TMAO, germ-free mice, and microbial transplantation collectively confirm a ...
Genome-wide atlas of gene expression in the adult mouse brain
Mice * Mice, Inbred C57BL * Organ Specificity * RNA, Messenger / genetics * RNA, Messenger / metabolism ... Genome-wide atlas of gene expression in the adult mouse brain Nature. 2007 Jan 11;445(7124):168-76. doi: 10.1038/nature05453. ... an anatomically comprehensive digital atlas containing the expression patterns of approximately 20,000 genes in the adult mouse ...
Expression of lymphocyte activation gene 3 (LAG-3) on B cells is induced by T cells
With the help of new monoclonal antibodies against mouse LAG-3, we show that LAG-3 surface expression is not lim … ... Mice, Inbred C57BL * Mice, Knockout * Rats * Rats, Inbred Lew * Signal Transduction / immunology* ... With the help of new monoclonal antibodies against mouse LAG-3, we show that LAG-3 surface expression is not limited to ...
IL-17a promotes sociability in mouse models of neurodevelopmental disorders
Mice, Inbred C57BL * Neurodevelopmental Disorders / immunology* * Pregnancy * Prenatal Exposure Delayed Effects * Social ... IL-17a promotes sociability in mouse models of neurodevelopmental disorders Nature. 2020 Jan;577(7789):249-253. doi: 10.1038/ ... LPS treatment in monogenic mutant mice did not induce amounts of interleukin-17a (IL-17a) comparable to those induced in MIA ... We compared an environmental model of neurodevelopmental disorders in which mice were exposed to maternal immune activation ( ...
Dietary simple sugars alter microbial ecology in the gut and promote colitis in mice
Mice, Inbred C57BL * Monosaccharides Substances * Dietary Sugars * Monosaccharides * Dextran Sulfate Grants and funding * P30 ... High-glucose-fed Il10-/- mice also developed a worsened colitis compared to glucose-untreated Il10-/- mice. Short-term intake ... Furthermore, germ-free mice colonized with microbiota from sugar-treated mice showed increased colitis susceptibility. Together ... mice. Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate ...
Dynamic changes in the cardiac methylome during postnatal development
... to determine whether DNA methylation plays an important role in guiding transcriptional changes during the first 2 wk of mouse ... Mice, Inbred C57BL * Mice, Inbred ICR * Myocardium / metabolism* * Myocytes, Cardiac / cytology * Myocytes, Cardiac / drug ... from P1 to P14 in the mouse]. The vast majority (~80%) of DMRs were hypermethylated between P1 and P14, and these ... to determine whether DNA methylation plays an important role in guiding transcriptional changes during the first 2 wk of mouse ...
Age-dependent epigenetic control of differentiation inhibitors is critical for remyelination efficiency
Mice * Mice, Inbred C57BL * Microglia / drug effects * Microglia / ultrastructure * Microscopy, Electron, Transmission / ... Defective remyelination can be recapitulated in vivo in mice receiving systemic administration of pharmacological HDAC ...
PGC1α drives NAD biosynthesis linking oxidative metabolism to renal protection
Mice * Mice, Inbred C57BL * Mitochondria / metabolism * NAD / biosynthesis* * Niacinamide / deficiency * Niacinamide / ... Inducible tubular transgenic mice (iNephPGC1α) recapitulate the effects of NAM supplementation, including more local NAD and ... Notably, exogenous NAM improves local NAD levels, fat accumulation, and renal function in post-ischaemic Pgc1α(-/-) mice. ... Following renal ischaemia, Pgc1α(-/-) (also known as Ppargc1a(-/-)) mice develop local deficiency of the NAD precursor ...
Azithromycin blocks autophagy and may predispose cystic fibrosis patients to mycobacterial infection
Mice, Inbred C57BL * Microtubule-Associated Proteins / genetics * Microtubule-Associated Proteins / metabolism * Mycobacterium ... treatment inhibited intracellular killing of mycobacteria within macrophages and resulted in chronic infection with NTM in mice ...
Early-onset aging and mitochondrial defects associated with loss of histone acetyltransferase 1 (Hat1)
To understand the broader biological role of Hat1, we have generated a conditional mouse knockout model of this enzyme. We ... Mice, Inbred C57BL * Mice, Knockout * Mitochondria / enzymology* * Mitochondria / pathology* Substances * Histone ... Hat1+/- mouse embryonic fibroblasts (MEFs) display modest increases in endogenous DNA damage but have significantly higher ... To understand the broader biological role of Hat1, we have generated a conditional mouse knockout model of this enzyme. We ...
AIM2 and NLRP3 inflammasomes activate both apoptotic and pyroptotic death pathways via ASC
Mice, Inbred C57BL * Mice, Knockout * NLR Family, Pyrin Domain-Containing 3 Protein ...
Laquinimod interferes with migratory capacity of T cells and reduces IL-17 levels, inflammatory demyelination and acute axonal...
The mice were treated at disease induction and after disease onset. Spinal cords were asse … ... cytokine profiles and migratory capacities of lymphocytes in C57BL/6 mice with active EAE induced with MOG(35-55) peptide. ... Mice * Mice, Inbred C57BL * Quinolones / therapeutic use* * T-Lymphocyte Subsets / drug effects ... cytokine profiles and migratory capacities of lymphocytes in C57BL/6 mice with active EAE induced with MOG(35-55) peptide. The ...
Immune requirements of post-exposure immunization with modified vaccinia Ankara of lethally infected mice
Mice * Mice, Inbred C57BL * Mice, Transgenic * Vaccines / therapeutic use* * Vaccinia virus / immunology* ... Immune requirements of post-exposure immunization with modified vaccinia Ankara of lethally infected mice PLoS One. 2010 Mar 11 ... By using various gene-targeted and transgenic mouse strains we show that NK cells, CD4 T cells, CD8 T cells and antibodies are ... Intravenous MVA immunization induced strong innate and adaptive immune responses in lethally infected mice. ...
Spatially-Resolved Proteomics: Rapid Quantitative Analysis of Laser Capture Microdissected Alveolar Tissue Samples
Mice * Mice, Inbred C57BL * Proteome / analysis* * Proteomics* * Tandem Mass Spectrometry Substances * Proteome ...
Social stress induces neurovascular pathology promoting depression
We investigated the effect of chronic social defeat stress, a mouse model of depression, on blood-brain barrier (BBB) ... Mice, Inbred C57BL * Nucleus Accumbens / pathology * Social Environment* * Stress, Psychological / pathology* * Stress, ... We investigated the effect of chronic social defeat stress, a mouse model of depression, on blood-brain barrier (BBB) ... Reduced BBB integrity in NAc of stress-susceptible or mice injected with adeno-associated virus expressing shRNA against Cldn5 ...
Discovery and Characterization of Human-Urine Utilization by Asymptomatic-Bacteriuria-Causing Streptococcus agalactiae
Mice, Inbred C57BL * Retrospective Studies * Streptococcus agalactiae / genetics * Streptococcus agalactiae / growth & ...
n-3 Fatty acids combined with flavan-3-ols prevent steatosis and liver injury in a murine model of NAFLD
Mice, Inbred C57BL * Non-alcoholic Fatty Liver Disease / pathology* * Non-alcoholic Fatty Liver Disease / prevention & control ... In a high-fat high-fructose (HF/HFr) fed C57Bl/6J mouse model, the independent and interactive impact of n-3 and FLAV on ...