18] The oocytes reaching the metaphase II stage were in vitro fertilized by ICSI and in vitrocultured up to the blastocyst ... At the end of in vitro maturation, 210 oocytes of the 319 (65.83%) collected from group 1 reached metaphase II stage (MII stage ... reached metaphase II stage and were fertilized by ICSI, which resulted in 199 cleaved embryos and 40 blastocysts (0.85 per OPU ... reached metaphase II after in vitro maturation. The matured oocytes were fertilized by ICSI. There were 74 (52%) cleaved ...

Kinetochore microtubule dynamics and the metaphase-anaphase transition. Zhai, Y., Kronebusch, P.J., Borisy, G.G. J. Cell Biol. ... rh-pha was microinjected during prometaphase or metaphase to label actin filaments that were present at that stage [12]. ... In mitotic cycles, however, Emi1 is destroyed in every pro-metaphase, and accordingly, it is unclear why Emi1 should be ... DCLK regulates the formation of bipolar mitotic spindles and the proper transition from prometaphase to metaphase during ...

Like animal cells, they go through the same stages of mitosis: prophase, metaphase, anaphase, and telophase, followed by ...

Students can view the changes that occur from a resting cell through early prophase, prophase, late prophase, metaphase, ...

2017) PRC1-labeled microtubule bundles and kinetochore pairs show one-to-one association in metaphase. EMBO Rep, 18(2): 217. ... Klemm, A., Bosilj, A., Glunčić, M., Pavin, N. & Tolić, I. (2018) Metaphase kinetochore movements are regulated by kinesin-8 ... Topic: Kinetochore oscillations in mitotic metaphase. Total amount granted: 256,000 EUR.. *2013 - 2015: Research grant from the ... one association in metaphase. EMBO reports, 18, 217-230 doi:10.15252/embr.201642650. ...

Fifteen unique metaphases were analyzed.. Figure 1-figure supplement 2 Download asset Open asset ...

Metaphase spreads from the patients were prepared according to standard procedures and at least 50 metaphases were analysed for ... Left: the entire metaphase of a patient with simple 22q13 deletion. Right: chromosomes 22 from patients with a simple deletion ... Despite the remarkable stability seen in metaphase for all patients, micronuclei were observed. These most often contained the ... Determining the size of the deletion using dual colour FISH on metaphases of patients with 22q13 partial monosomy. ...

The bright cell with lots of orange on the right is in metaphase and the cell with lots of green on the left is in anaphase. ... Metaphase and anaphase are both stages in mitosis and we study the mechanisms that govern them. Cell division is crucial for ... The drug we are developing stops cancer cells from continuing to divide and arrests cells in metaphase. The mechanism it ...

In all analyses, 15 individuals were used, and 10 metaphases were examined on average per slide. Images were obtained under an ... 1986], with some modifications: metaphase chromosomes were denatured in 70% formamide/2× SSC at 75°C for 5 min; the probes were ... For further molecular cytogenetic analyses, metaphase chromosomes were obtained as previously described by Imai et al. [1988] ... which was found in all metaphase chromosomes of the examined individual bees (Fig. 2; Table 3). Both species had 1 colony with ...

The molecular architecture of the meiotic spindle is remodeled during metaphase arrest in oocytes. Costa, M. F. A. & Ohkura, H. ...

Typical metaphase chromosomes from the hybrid andromerogones of H. pulcherrimus non-nucleate egg fragments and sperm of four ... Figs 1a-d show typical metaphase chromosomes from the hybrid andromerogones obtained by inseminating H. pulcherrimus non- ... and clear metaphase plates of male chromosomes may be obtained from hybrid andromerogones for many species. ...

Metaphase cells were fixed and stained with CREST sera (red, Alexa Flour 568), CenpI antibody (green, Alexa Fluor... ... Metaphase cells were fixed and stained with CREST sera (red, Alexa Flour 568), CenpI antibody (green, Alexa Fl... ...

Dive into the research topics of Silencing Cenp-F weakens centromeric cohesion, prevents chromosome alignment and activates the spindle checkpoint. Together they form a unique fingerprint. ...

Aurora B kinase activity peaks at the transition from metaphase to the end of mitosis. Aurora B associates with chromosomes ...

E) Fixed sample of metaphase cells in MD1 stained for tubulin (left), E-cadherin (center), and Pins (right). (F) Fixed sample ... 2008) Lis1/dynactin regulates metaphase spindle orientation in Drosophila neuroblasts Developmental Biology 319:1-9. ...

To determine the percentage of euploid metaphase chromosomes in the targeted ES cells. At least 20 spreads are counted.. ...

To determine the percentage of euploid metaphase chromosomes in the targeted ES cells. At least 20 spreads are counted.. ...

Fluorescence in Situ Hybridization (FISH) Utility for Risk Score Assessment in Patients With MDS With Normal Metaphase ...

Karyotype analysis of several bone marrow aspirates disclosed, besides both normal metaphases and metaphases containing... ...

Extrachromosomal gene amplification in acute myeloid leukemia; characterization by metaphase analysis, comparative genomic ...

This happens until all chromosomes are aligned at the metaphase plate. In addition to this interaction, MAD2 also interacts ...

Tests for genotoxicity included the Ames mutation assay, the metaphase analysis of human lymphocytes, and the mouse ...

molecular (PCR), metaphase cytogenetics, or FISH. - FLT3: ITD or a point mutation in the TKD loop of variant allele fractions ≥ ...

Metaphase chromosomes are highly extensible objects that return to their native shape after being stretched up to 10 times ...

... a comparison of data obtained by fluorescence in situ hybridization in interphase and metaphase. Environ. Mol. Mutagen. 34, 260 ...

8B) (Munro et al., 2004). During metaphase, NMY-2::GFP was mostly restricted to the anterior half of the embryo, although there ...

Cytogenetic response is usually done on bone marrow samples in metaphase (at the time of diagnosis and if failure to achieve ... Partial cytogenetic response (PCyR): Presence of Ph+ cells in 1%-35% metaphases ...

... with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a ...