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*  Influence of α-crystallin Racemization on Lens Membrane Fluidity | IOVS | ARVO Journals
Purpose: To evaluate the influence of α-crystallin racemization on lens membrane fluidity using the mild heating model based on lens epithelial cells (LECs).. Methods: LECs (SRA01/04) heated at 500C were used as the mild heating model to simulate the ageing process in vitro. After incubation under 500C for 0min, 15min, 30min, 45min and 60min, α-crystallins in LECs were isolated using gel filtration chromatography and processed with acidolysis. The purified samples were treated with o-phtalaldehyde and N-tertbutyloxycarbonyl-L-cysteine for pre-column derivatization before racemization analysis using reversed-phase high performance liquid chromatography (RP-HPLC). To detect the α-crystallin racemization, we calculated the D/L (D/L amino-acid residual) ratio according to the peak area in each chromatogram. Laurdan staining and two-photon confocal microscopy were applied to analyze the lens membrane fluidity of each group with different heating ...
http://iovs.arvojournals.org/article.aspx?articleid=2335631
*  PLOS ONE: Membrane Association of the PTEN Tumor Suppressor: Molecular Details of the Protein-Membrane Complex from SPR Binding...
The structure and function of the PTEN phosphatase is investigated by studying its membrane affinity and localization on in-plane fluid, thermally disordered synthetic membrane models. The membrane association of the protein depends strongly on membrane composition, where phosphatidylserine (PS) and phosphatidylinositol diphosphate (PI(4,5)P2) act pronouncedly synergistic in pulling the enzyme to the membrane surface. The equilibrium dissociation constants for the binding of wild type (wt) PTEN to PS and PI(4,5)P2 were determined to be Kd∼12 µM and 0.4 µM, respectively, and Kd∼50 nM if both lipids are present. Membrane affinities depend critically on membrane fluidity, which suggests multiple binding sites on the protein for PI(4,5)P2. The PTEN mutations C124S and H93R show binding affinities that deviate strongly from those measured for the wt protein. Both mutants bind PS ...
http://journals.plos.org/plosone/article/related?id=10.1371/journal.pone.0032591
*  Adult DHA Gummy Fruits | Neuro By Nature ™
Adult DHA Gummy Fruits were designed for adults who don't like to swallow large fish oil capsules. These gummies contain 225mg of fish oil, 50mg of DHA (docosahexaenoic acid) and 10mg of EPA (eicosapentaenoic acid). These tasty gummies come in orange, lemon and strawberry banana flavors that have no fishy aftertaste. Most of us in the U.S. don't get enough of these essential omega fatty acids because we don't have enough oily fishes in our diets. These essential fatty acids are found in fish such as anchovies, sardines, salmon and mackerel. For those of you who don't like fish, Coromega's DHA Gummy for Adults is the answer for you.. Thousands of clinical and scientific studies have reported that these essential omega fatty acids support heart, brain, eye and joint health. These fatty acids are actually important for every cell in the body. They become integral parts of the plasma membrane and are important for maintaining cell membrane fluidity, ion flows, ...
http://neurobynature.com/products/adult-dha-gummy-fruits-review/
*  Transcriptome responses of Lactobacillus acetotolerans F28 to a short and long term ethanol stress----中国科学院微生物研究所
Lactobacillus acetotolerans is a major microbe contributing to the Chinese liquor fermentation with unknown function. It can be grown well in a high concentration of ethanol. RNA sequencing (RNA-seq) was performed on L. acetotolerans F28 growing in 12% ethanol to determine important genetic mechanisms for both a short and long term adaption to this environment. A genome-wide transcriptional analysis revealed that the most important genetic elements for L. acetotolerans F28 grown in ethanol are related to high levels of stress response and fatty acid biosynthesis, and a reduction of amino acid transport and metabolism after both a short and long time stress. The fatty acid methyl ester analyses showed that most fatty acids were increased in L. acetotolerans F28 after exposure to ethanol while the unsaturated fatty acid octadecenoic acid (C18: 1) was significantly increased. The increasing unsaturated fatty acid biosynthesis in L. acetotolerans F28 might enhance cell membrane ...
http://sourcedb.im.cas.cn/chinese/thesis/201708/t20170813_4844224.html
*  Dietary Fatty Acids and the Risk of Hypertension in Middle-Aged and Older Women | Hypertension
In this large-scale prospective cohort study of middle-aged and older women, intake of SFAs, MUFAs, and trans FAs was each positively associated with risk of hypertension. The associations for SFAs and MUFAs were largely attenuated by adjustment for potential intermediate factors, including BMI, diabetes mellitus, and hypercholesterolemia, whereas the associations for trans FAs remained significant even after these adjustments. Intake of PUFAs was generally not associated with risk of hypertension.. Various fats potentially have different effects on BP. Experimental studies found that feeding rats with SFAs resulted in impaired endothelial function7 and enhanced sympathetic nervous system activities,8 which will increase BP. In contrast, consumption of long-chain ω3 PUFAs modulated plasma phospholipid composition and cell membrane fluidity, increased the production of vasodilators, and reduced cardiac adrenergic activity,5,35 which will lower BP. The incorporation of ω6 ...
http://hyper.ahajournals.org/content/56/4/598
*  Foods for Thought - Natural Brain Supplements - Healthy Living Benefits
If this could seem like a matter of detail, imagine that cognitive functions can be severely influenced by the metabolic process as a whole, due to the disproportionate amount of energy the brain requires just to sustain the survival mechanisms, not to mention its "higher" functions. Metabolic dysfunctions can thus translate into rational ones. At the micro level, this is a consequence of the problems that neurons have in the distribution of energy, leading to affections in synaptic plasticity, which starts the vicious circle all over again. To relieve the strain, omega-3 acids are a known stimulant of the said plasticity.[4]. Furthermore, numerous patients with mental illnesses (ranging from epilepsy to Alzheimer's) exhibit modifications in energy homeostasis, therefore at least a better management of these disorders may become a reality shortly with the aid of correct dietary provisions.. Primarily through the regulation of neurotransmitter pathways and cell membrane ...
https://www.hlbenefits.com/foods-thought-natural-brain-supplements/
*  Detox and Weightloss
A common benefit of a successful detox program is weight loss. Our fat cells contain stored toxins, including heavy metals and excess hormones, so it is especially important to encourage functional detox pathways as fat cells are released when losing weight. While undergoing a cleanse, other important physiological considerations include:. *Cell membrane fluidity to allow toxins to exit the cell for removal by the liver, kidneys, and digestive tract ...
https://www.mysparkhealth.com/detox-and-weightloss/
*  PLOS ONE: Rho Signaling Participates in Membrane Fluidity Homeostasis
Preservation of both the integrity and fluidity of biological membranes is a critical cellular homeostatic function. Signaling pathways that govern lipid bilayer fluidity have long been known in bacteria, yet no such pathways have been identified in eukaryotes. Here we identify mutants of the yeast Saccharomyces cerevisiae whose growth is differentially influenced by its two principal unsaturated fatty acids, oleic and palmitoleic acid. Strains deficient in the core components of the cell wall integrity (CWI) pathway, a MAP kinase pathway dependent on both Pkc1 (yeast's sole protein kinase C) and Rho1 (the yeast RhoA-like small GTPase), were among those inhibited by palmitoleate yet stimulated by oleate. A single GEF (Tus1) and a single GAP (Sac7) of Rho1 were also identified, neither of which participate in the CWI pathway. In contrast, key components of the CWI pathway, such as Rom2, Bem2 and Rlm1, failed to influence fatty acid sensitivity. The ...
http://journals.plos.org/plosone/article/related?id=10.1371/journal.pone.0045049
*  Tb(III) functionalized vesicles for phosphate sensing: Membrane fluidity controls the sensitivity - Publikationsserver der...
We report a modular design of vesicular chemosensors by co-embedding a Tb(III) complex and a receptor-sensitizer conjugate in phospholipid vesicles. The binding of phosphate anions to the vesicle surface in aqueous media is detected by a decrease in Tb(III) phosphorescence. The sensory response can be modulated by a variation in the membrane fluidity. ...
https://epub.uni-regensburg.de/28374/
*  Plus it
New therapeutic targets that can inhibit cancer metastasis are desperately needed. To address this issue, we have leveraged a computational approach and performed an unbiased screen for therapies that can inhibit an epithelial-to-mesenchymal transition (EMT) gene expression profile, a phenotype that is believed to promote metastases. This yielded predictions of drugs that can both promote and inhibit an EMT transcriptional profile. Following up on these predictions, we confirmed that these drugs can induce or repress an EMT in a range of breast cancer cell lines. Furthermore, we discovered that they do so by altering the fluidity of the plasma membrane. An increase in plasma membrane fluidity is necessary and sufficient to induce and maintain an EMT state, increased motility, and increased mammosphere forming ability. Therapies that decrease fluidity can inhibit metastasis in xenograft mouse models. Finally, we found that ...
http://cancerres.aacrjournals.org/content/75/15_Supplement/4984
*  Alteration of lymphocyte and erythrocyte membrane microviscosity in the blood of tumor patients
Abstract: Lymphocyte and erythrocyte membrane microviscosites oflipid-protein contact zones and lipid bilayer in blood ofpatients with gastric, urinary bladder and ovary cancers wereinvestigated in comparison with healthy volunteers by means ofcoefficients of eximerization of fluorescent probe pyrene. Thedecrease of membrane fluidity (i.e. the increase ofmicroviscosity) was revealed in blood cell membranes of patientswith diffuse cancers of studied localizations. In the case ofovary cancer membrane fluidity decreased significantly only inpatients with ascite form of the disease. The most pronouncedincrease of microviscosity was observed in lymphocytes andespecially in erythrocytes of practically incurable patients.Interrelationship between microviscosity and other processes thatinfluence on structural properties of membranes under malignancyand their functional role are discussed ...
http://pbmc.ibmc.msk.ru/index.php/en/article/PBMC-1999-45-1-53-en
*  Change in zinc permeability of lipid bilayers as a function of fluidity and oxidation
The main goal in my project was find out if the rate of zinc crossing the bilayer was either due to the fluidity of vesicles or the level of oxidation of the vesicles.To measure the oxidation a simple procedure called the TBA Test was used to measure each PC tested. The fluidity measurement was a calculation using the temperature the vesicles went from gel to liquid crystalline phase and the experimental temperature.Measuring the rate at which zinc crossed the bilayer was done using spectral changes that occur as zinc binds with APIII, a metal chelator entrapped inside the vesicles. To measure these rates we used k', the rate constant at which zinc is crossing the bilayer at a certain concentration and k, the second order diffusion rate constant which is the slope of k' vs. [Zn].The rates at which zinc was crossing the bilayer for each PC was then compared to the fluidity and oxidation levels for each PC. There was no direct correlation between the rates and ...
http://cardinalscholar.bsu.edu/handle/handle/185943
*  Plus it
To evaluate the relative contributions of three possible mechanisms that can be advanced to explain the observation that hyperoxia decreases serotonin uptake by endothelial cells, we examined the effect of high O2 tensions on Na+-K+-ATPase activity, ATP content, and plasma membrane fluidity in cultured endothelial cells. Confluent monolayers of pulmonary artery and aortic endothelial cells were exposed to 95% O2 (hyperoxia) or 20% O2 (controls) in 5% CO2 at 1 ATA for 4-42 h. Exposure to high O2 tensions had no effect on Na+-K+-ATPase activity or ATP content in pulmonary artery or aortic endothelial cells in culture. However, hyperoxia decreased the fluidity of the plasma membrane of pulmonary artery and aortic endothelial cells in culture, and the time course for the decrease in fluidity parallels that of the hyperoxic inhibition of serotonin transport. These results indicate that hyperoxia decreases ...
http://jap.physiology.org/content/60/3/826
*  Membrane fluidity | Physics Forums - The Fusion of Science and Community
Why exactly does the cell membrane become more fluid in higher temperatures than lower? Does it have anything to do with the degree of saturation ...
https://www.physicsforums.com/threads/membrane-fluidity.130125/
*  ASMscience | Perception and Transduct
This chapter focuses on the various mechanisms by which membrane fluidity is modulated in bacteria vis-à-vis its importance in cold adaptation. A detailed update on the perception and transduction of low-temperature signals in bacteria is also included. Subsequently, it was found that trans-monounsaturated are predominant in gram-negative bacteria and are synthesized by direct isomerization of cis-unsaturated fatty acids to trans-unsaturated fatty acids without shifting of a double bond. One of the predominant signal transduction mechanisms employed by bacteria is the phosphotransfer pathway commonly referred to as the two-component signal transduction system, which consists of a sensor kinase (histidine kinase) and a response regulator, found in bacteria, Archaea, and Eukarya. The first direct evidence for the two-component signal transduction mechanism involved in sensing cold has come from studies on Bacillus subtilis. Modulation in membrane ...
http://www.asmscience.org/content/book/10.1128/9781555815813.ch15
*  Mitochondrial membrane fluidity and oxidative damage to mitochondrial DNA in aged and AD human brain.
Abstract Oxidative damage on biological molecules has been proposed as a major cause of alterations observed in aging brain as well as in neurodegenerative diseases. In this study,..
https://www.omicsonline.org/references/mitochondrial-membrane-fluidity-and-oxidative-damage-to-mitochondrial-dna-in-aged-and-ad-human-brain-821362.html
*  Membrane Fluidity Gradient is Altered in Diabetic Mononuclear Leukocytes | Clinical Science
Thank you for your interest in spreading the word about Clinical Science.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
http://www.clinsci.org/content/86/s30/18P.3
*  Membrane fluidity by flow cytometry - Flow Cytometry
Could speculate about come FRET-FRAP approchaes but thes stuff is mainly addressed to imaging or to spectrofluorimetric analysis.. ...
http://www.protocol-online.org/biology-forums-2/posts/19362.html
*  Lecture of Timothy J. Healey | Budapest University of Technology and Economics
Lecturer: Timothy J. Healey. Title: Global symmetry-breaking bifurcation in a model for 2-phase lipid-bilayer vesicles - analysis and computation. Abstract: We study a model for lipid-bilayer membrane vesicles exhibiting phase separation, incorporating a phase field together with membrane fluidity and bending elasticity. We prove the existence of a plethora of equilibria in the large, corresponding to symmetry-breaking solutions of the Euler-Lagrange equations. We also numerically compute a special class of such solutions, namely those possessing icosahedral symmetry. We overcome several difficulties along the way. Due to inherent surface fluidity combined with finite curvature elasticity, neither the Eulerian (spatial) nor the Lagrangian (material) description of the model lends itself well to analysis. This is resolved via a singularity-free radial-map description, which effectively eliminates the grossly under-determined mid-plane ...
http://www.bme.hu/node/4136?language=en
*  New drug for autism? - AutismWeb Forum
The cell danger response (CDR) is the evolutionarily conserved metabolic response that protects cells and hosts from harm. It is triggered by encounters with chemical, physical, or biological threats that exceed the cellular capacity for homeostasis. The resulting metabolic mismatch between available resources and functional capacity produces a cascade of changes in cellular electron flow, oxygen consumption, redox, membrane fluidity, lipid dynamics, bioenergetics, carbon and sulfur resource allocation, protein folding and aggregation, vitamin availability, metal homeostasis, indole, pterin, 1-carbon and polyamine metabolism, and polymer formation. The first wave of danger signals consists of the release of metabolic intermediates like ATP and ADP, Krebs cycle intermediates, oxygen, and reactive oxygen species (ROS), and is sustained by purinergic signaling. After the danger has been eliminated or neutralized, a choreographed sequence of anti-inflammatory and regenerative ...
http://www.autismweb.org/forum/viewtopic.php?f=4&t=30356
*  Suramin reverses autism in mice - AutismWeb Forum
The cell danger response (CDR) is the evolutionarily conserved metabolic response that protects cells and hosts from harm. It is triggered by encounters with chemical, physical, or biological threats that exceed the cellular capacity for homeostasis. The resulting metabolic mismatch between available resources and functional capacity produces a cascade of changes in cellular electron flow, oxygen consumption, redox, membrane fluidity, lipid dynamics, bioenergetics, carbon and sulfur resource allocation, protein folding and aggregation, vitamin availability, metal homeostasis, indole, pterin, 1-carbon and polyamine metabolism, and polymer formation. The first wave of danger signals consists of the release of metabolic intermediates like ATP and ADP, Krebs cycle intermediates, oxygen, and reactive oxygen species (ROS), and is sustained by purinergic signaling. After the danger has been eliminated or neutralized, a choreographed sequence of anti-inflammatory and regenerative ...
http://www.autismweb.org/forum/viewtopic.php?f=6&t=32423&p=219848
*  Membranes, Fluidity of - CHEMICAL BIOLOGY
... - reflects the multidimensional character of chemical biology, focusing in particular on the fundamental science of biological structures and systems, the use of chemical and biological techniques to elucidate
http://schoolbag.info/chemistry/chemical_biology/89.html
*  Jarrow Formulas - Jarrow - PS-100 100MG - 60 Softgels
Phosphatidylserine USAGE: Take 1 - 3 capsules daily with food, or as directed by your qualified health consultant. Best kept refrigerated. Phosphatidylserine (PS) is a natural phospholipid that is an essential component of cell membranes. Cogni-PS promotes brain function by increasing neuronal membrane fluidity (cell-to-cell communication), resulting in improved cognition. Also, Cogni-PS protects against stress by mitigating the actions of cortisol (catabolic stress hormone.) Cogni-PS is a concentrated form of phosphatidylserine derived from an enzymatic process of soy phospholipids. Keep out of the reach of children. SUPPLEMENT FACTS Serving Size 1 Capsule Amount % DV -------------------------------------------------------------------------------- Phosphatidylserine (PS) 100 mg * -------------------------------------------------------------------------------- Phosphatidylcholine (PC) 12 mg ...
http://www.totaldiscountvitamins.com/product/6272/jarrow_formulas
*  Jarrow - PS-100 100MG - 60 Caps
PhosphatidylserineUSAGE: Take 1 - 3 capsules daily with food, or as directed by your qualified health consultant. Best kept refrigerated. Phosphatidylserine (PS) is a natural phospholipid that is an essential component of cell membranes. Cogni-PS™ promotes brain function by increasing neuronal membrane fluidity (cell-to-cell communication), resulting in improved cognition. Also, Cogni-PS™ protects against stress by mitigating the actions of cortisol (catabolic stress hormone.)Cogni-PS™ is a concentrated form of phosphatidylserine derived from an enzymatic process of soy phospholipids.Keep out of the reach of children.SUPPLEMENT FACTSServing Size 1 CapsuleAmount % DV--------------------------------------------------------------------------------Phosphatidylserine (PS) 100 mg *--------------------------------------------------------------------------------Phosphatidylcholine (PC) 12 mg ...
https://www.totaldiscountvitamins.com/create_review/6270/jarrow_formulas
*  Rigidification of MHC-I/peptide complexes is an essential step for enhanced T cell recognition of cancer-associated neo...
Adnane Achour abstract presented on Rigidification of MHC-I/peptide complexes is an essential step for enhanced T cell recognition of cancer-associated neo-epitopes associated with impaired processing at Molecular Immunology 2017 | Conferenceseries Ltd
https://molecularimmunology.conferenceseries.com/abstract/2017/rigidification-of-mhc-i-peptide-complexes-is-an-essential-step-for-enhanced-t-cell-recognition-of-cancer-associated-neo-epitopes-associated-with-impaired-processing