Conjugation to lysine residues in human serum albumin (HSA) gave desired SNAFL/HSA conjugates. Carbodiimide activation of EBIO- ... These dextrans had a molecular weight of about 10,000 1.8 fluorophores per conjugate, and 2.2 lysines per conjugate and are ... In one embodiment, the spacer material is human serum albumin (HSA) having ˜44 lysine residues available for covalent ... Fluorescein labeled dextrans with "fixable" lysine residues were obtained from Molecular Probes. ...

Both induce nucleophilic attack of the amino group of lysine and their subsequent covalent bonding via the crosslinker. However ... lysine. In some embodiments, the invention is used with glutaraldehyde. While it is believed to operate by a similar mechanism ... lysine in 0.1 M phosphate buffer; and (d) 4% paraformaldehyde with 0.05% glutaraldehyde (electron microscopy ...