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*  Open tubular liquid chromatography using a sol-gel derived stationary phase | SpringerLink
... was fabricated by the sol-gel process and coated onto the inner walls of fused silica capillaries. The coating was used as... ... The high surface area of the coating, characteristic of materials fabricated by the sol-gel process, resulted in capillary ... The coating was used as the stationary phase for reversed-phase open tubular liquid chromatography (OTLC). The sol-gel process ... Column liquid chromatography Open tubular columns Sol-gel coatings Laser-induced fluorescence detection ...
https://link.springer.com/article/10.1007/BF02292997
*  biopreservation: Topics by WorldWideScience.org
plantarum 163 was purified by salt precipitation, gel filtration, and reverse-phase high-performance liquid chromatography (RP- ... the characteristics of their biosynthesis and production in fermentation systems, the characteristics of the known pediocin ... polyacrylamide gel electrophoresis (SDS-PAGE). Reverse-phase high-performance liquid chromatography (HPLC) analysis showed a ... gel filtration chromatography and high performance liquid chromatography. Its molecular weight was estimated at 4.1 kDa using ...
https://worldwidescience.org/topicpages/b/biopreservation.html
*  Immunochemical detection and characterisation of osteocalcin f...
... has been fractionated by gel filtration chromatography and reversed-phase high performance liquid chromatography, and behaves ... in a manner characteristic of osteocalcin from modern bones. Carbon-14 dating of bones and gizzard contents found in ...
https://www.mysciencework.com/publication/show/immunochemical-detection-characterisation-osteocalcin-from-moa-bone-2f353c85
*  Partition chromatography - Wikipedia
"Liquid Chromatography with Hydrocarbonaceous Bonded Phases; Theory and Practice of Reversed Phase Chromatography". Journal of ... and gas-liquid chromatography which is more commonly known as gas chromatography. The modification of silica gel stationary ... phase lead to many creative ways of modifying stationary phases in order to influence the separation characteristics. The most ... In liquid-liquid separation, a compound is distributed between two immiscible liquid phases under equilibrium conditions. ...
https://en.wikipedia.org/wiki/Partition_chromatography
*  Identification of Tetraspanin-7 as a Target of Autoantibodies in Type 1 Diabetes | Diabetes
Eluates from gel regions equivalent to 38 kDa were analyzed by liquid chromatography-tandem mass spectrometry for protein ... Peptides were resolved by reversed-phase chromatography on a 75-μm C18 EASY column using a linear gradient of acetonitrile in ... Tspan7 is a member of the tetraspanin family, members of which share structural characteristics of four transmembrane domains, ... In-Gel Trypsin Digestion and Mass Spectrometry of 38,000 Mr Proteins. Gel slices representing 38,000 Mr regions of all samples ...
http://diabetes.diabetesjournals.org/content/65/6/1690?ijkey=b2dc4034203efa847df7bd2b145c00242795fb8d&keytype2=tf_ipsecsha
*  Analysis of xanthyletin and secondary metabolites from Pseudomonas stutzeri ST1302 and Klebsiella pneumoniae ST2501 against...
The eluent was collected and fractions defined according to their thin layer chromatography (TLC) characteristics. TLC was ... pneumoniae ST2501 were fractionated by activity-guided separation liquid column chromatography (LCC). The extracts were applied ... Gradient mixtures of dichloromethane and methanol were used as mobile phases. ... using silica gel GF254 pre-coated aluminum sheets. The fractions containing the same major compounds were combined and tested ...
https://link.springer.com/article/10.1186%2Fs12866-019-1452-4
*  Lecture 8: Protein purification - ppt video online download
These are sometimes called resins when used in liquid chromatography. The stationary phase has certain physical and chemical ... Common types of chromatographic stationary phases Ion exchange Hydrophobic Gel filtration Affinity ... characteristics that allow it to interact in various ways with different proteins. ... The mixture of substances (proteins) to be fractionated is dissolved in a liquid or gaseous fluid called the mobile phase. This ...
http://slideplayer.com/slide/4335435/
*  Physical Stability of a Gel Filtration Column for Antibody Analysis | Sigma-Aldrich
Independent of the mode of liquid chromatography, the lifetime of an HPLC or UHPLC column depends on the characteristics of the ... Particle structure, bonded phase composition, number of injections, mobile phase, pH, flow rate, sample type/mass/volume, ... When it comes to gel filtration chromatography, particle stability and thus column stability is more of an issue than in ... Faster Protein and Peptide Liquid Chromatography: Introducing BIOshell Fused-Core HPLC Columns ...
https://www.sigmaaldrich.com/technical-documents/articles/analytical/pharmaceutical/gfc-antibody-analysis.html
*  Symposium P | 1998 MRS Spring Meeting | San Francisco
MORPHOLOGICAL AND PHASE EVOLUTION CHARACTERISTICS OF IRON OXIDE ULTRAFINE PARTICLES AS A FUNCTION OF BURNER CONDITIONS DURING ... In a gel at 1% solids, the pore size was approximately 100 nm. At this concentration gels can easily be generated in seconds; ... The precipitates formed using interface of gas-liquid phase were decomposed into fine spherical zirconia powder of high purity ... the pore space of which is unusually open and useful for applications such as protein chromatography. 11:30 AM P3.6 EFFECT OF ...
https://www.mrs.org/spring1998/symposium-p
*  Endothelial Protein C-Targeting Liposomes Show Enhanced Uptake and Improved Therapeutic Efficacy in Human Retinal Endothelial...
The reaction was followed by thin-layer chromatography using a chloroform:methanol (95:5) mobile phase. PP appeared as a ... Purity was determined by both analytic high pressure liquid chromatography (HPLC) and 1H nuclear magnetic resonance (NMR) ... Liposome characteristics. (A) Cryo-TEM image of nonloaded (EPCR-NL) and PH-loaded EPCR-targeting (EPCR-NL1) liposomes. Scale ... Crude product was redissolved in a chloroform:methanol (95:5) mixture and purified on a silica gel column by using a chloroform ...
https://iovs.arvojournals.org/article.aspx?articleid=2679337
*  Dietary Intake and Plasma Levels of Choline and Betaine in Children with Autism Spectrum Disorders
... μL were deprotenized with three volumes of acetonitrile and further analyzed using normal phase chromatography on silica gel ... Choline and betaine concentrations were measured using a Dionex High Performance Liquid Chromatography-Ultraviolet System ... Participant Characteristics. Among the 288 ASD participants, 86.1% were male, 25.7% (74) were in the 1-3-year-age category, ... and dimethylglycine in plasma by a high-throughput method based on normal-phase chromatography-tandem mass spectrometry," ...
https://www.hindawi.com/journals/aurt/2013/578429/
*  Number 6
Sister-Line IR Rice Varieties by Polyacrylamide Gel Electrophoresis and Reversed-Phase High-Performance Liquid Chromatography. ... Effect of Cultivar and Environment on Quality Characteristics of Spring Wheat.. 0. M. Lukow and P. B. E. Mc Vetty.. VIEW ...
http://aaccnet.org/publications/cc/backissues/1991/Pages/Number6.aspx
*  Plasma Molecular Signatures in Hypertensive Patients With Renin-Angiotensin System SuppressionNovelty and Significance |...
In the validation phase, 105 patients were classified in the same groups as previously described. The clinical characteristics ... followed by liquid chromatography-tandem mass spectrometry according to previous publications from our group,17 and (3) a ... 2-dimensional fluorescence difference gel electrophoresis (2D-DIGE; GE-Healthcare) and isobaric tags for relative and absolute ... Demographic Data and Clinical Characteristics of the Study Population Recruited for the Validation Phase ...
http://hyper.ahajournals.org/content/68/1/157
*  Regulation of Intestinal α-Defensin Activation by the Metalloproteinase Matrilysin in Innate Host Defense | Science
3A) (23). Further analysis of MAT−/− extracts by reversed-phase high-performance liquid chromatography (rpHPLC) verified that ... we analyzed small intestinal extracts by acid-urea polyacrylamide gel electrophoresis (AU-PAGE). In this acidic gel system, ... characteristic of cryptdin-1) for the methionine normally at position 73 in procryptdin-15. A 40-base pair fragment encoding ... In a liquid-phase assay measuring bactericidal activity, MAT−/− crypt supernatants killed a defensin-sensitive strain of ...
https://science.sciencemag.org/content/286/5437/113?ijkey=487a6ee8099c810179f16707c3c4cd8b3ea6451c&keytype2=tf_ipsecsha
*  Patent US4746558 - Magnetic recording medium - Google Patents
... wherein a porous gel functioning as a molecular sieve is filled in a column whereby liquid chromatography is conducted. For the ... Now, the characteristics of the media wherein the magnetic layers 1 and 2 were replaced, respectively, by the magnetic layers 1 ... it is possible to use super-fine particulate aluminum oxide and titanium oxide which can be produced by a vapour phase method ... GPC (Gel Permeation Chromatography) is a method of separating molecules in a test sample depending upon their sizes in a mobile ...
http://www.google.com/patents/US4746558?dq=3657699
*  Patente US20020070338 - Ion mobility spectrometer incorporating an ion guide in combination with an ... - Google Patentes
... so as to trap and hold ions after separation based on mobility characteristics. ... For example, liquid chromatography involves passing a sample in a liquid phase through a chromatography column. The column is ... and then a second electrophoresis separation step in a second gel having different characteristics, intended to separate out ... In liquid chromatography and electrophoresis, there have been proposals which involve taking a sample, subjecting it to a first ...
http://www.google.es/patents/US20020070338?dq=flatulence
*  Topics - Steroids :: Chromatography Online
The steroid hormones have been separated by liquid chromatography using a silica gel column, 25 cm long, 4.6 mm I.D. packed ... The mobile phase consisted of a mixture of 76%v/v n-hexane and 24%v/v 2-propyl alcohol and was used at a flow rate of 1 ml/min ... Anabolic steroids are muscle-building substances, whereas androgenic steroids increase the masculine characteristics of an ...
http://chromatography-online.org/topics/steroids.php
*  Biosimilars | P&T Community
... and gel electrophoresis and reverse-phase, high-performance liquid chromatography (HPLC) can be used to identify disparities in ... including structural characteristics.3 The analytical data required for this purpose could include studies showing the amino ... and size exclusion chromatography, can determine the molecular weight and size differences between biosimilars and innovator ... leaving open the possibility of overlooking characteristics of the proposed biosimilar that may signal safety or other problems ...
https://www.ptcommunity.com/journal/article/full/2013/5/270/biosimilars
*  Protein adducts at critical protein sites as markers of toxicological risk - Enlighten: Theses
Spots were excised from the 2d gel digested and analysed by reversed phase liquid chromatography mass spectrometry. The IDA ... Results indicated that a characteristic conjugate specific ion at 359.1 Da could be detected for each of the peptides. This ... A print out of the image was used as a map for the excision of modified proteins from a duplicate gel. Digestion and LCMS ... Western blotting was carried out on a 2d gel run from an assay sample. The Western membrane was probed using an HRP- ...
http://theses.gla.ac.uk/4886/
*  N-acyl phosphatidylethanolamine-specific phospholipase D - Wikipedia
... and peptides from it were separated from one another by reverse phase high performance liquid chromatography. The resulting ... This band was cut out from the gel and digested with trypsin, ... whose characteristics were shown to be similar to those of the ... Measurement of the enzyme activity was done by thin layer chromatography of a radioactive substrate sensitive to the NAPE-PLD ... The integral membrane proteins were then solubilized using octyl glucoside and subjected to four column chromatography steps ( ...
https://en.wikipedia.org/wiki/N-acyl_phosphatidylethanolamine-specific_phospholipase_D
*  Patent US7763635 - Once daily dosage forms of trospium - Google Patents
The method used herein for the plasma concentration determination was the liquid chromatography/mass spectrometry/mass ... With this technique, trospium is extracted from an aliquot of plasma using a solid phase extraction procedure. This extract is ... In addition, there can be flow aids such as fumed silicon dioxide, silica gel, magnesium stearate, calcium stearate or any ... limited absorption window and bioavailability characteristics as trospium. Examples of such compounds include, for instance, ...
http://www.google.com/patents/US7763635?dq=6188988
*  Introduction to Pharmaceutical Chemical Analysis | Analytical Chemistry | Chemistry | Subjects | Wiley
11.2.6 Summary of Normal Phase Chromatography 147. 11.3 Reversed Phase Chromatography 148 ... on development and applications of artificial liquid membranes, and on electrokinetic separation methods. SPB has published ... 19 Analytical Chemical Characteristics of Selected Drug Substances 299. 19.1 Amitriptyline and Mianserin 299 ... 17.2 Paper Chromatography 263. 17.3 Supercritical Fluid Chromatography 264. 17.4 Gel Electrophoresis 265 ...
https://www.wiley.com/en-us/Introduction+to+Pharmaceutical+Chemical+Analysis-p-9781119282037
*  LARGE SCALE DISPOSABLE BIOREACTOR - Patent application
0190]Reverse Phase High Performance (Pressure) Liquid Chromatography (RP-HPLC): RP-HPLC comprises the separation of intact ... according to standard gel separation protocols. Briefly, the SDS gels consist of a stacking gel (3%) and a resolving gel (12 ... The characteristic peak and retention time of the plant recombinant GCD (prGCD) was determined using a column of stationary ... 0243]Reverse Phase High Performance (Pressure) Liquid Chromatography (RP-HPLC): Further characterisation of the proteins ...
http://www.patentsencyclopedia.com/app/20100112700
*  Repositorio da Producao Cientifica e Intelectual da Unicamp: Efeitos da irradiação na composição e propriedades funcionais da...
... reversed-phase high performance liquid chromatography (RP-HPLC) and of size exclusion high performance liquid chromatography ( ... The structural characteristics of SPIs were evaluated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl ... showed by reversed-phase high performance liquid chromatography profile, for all the isolated after four months of storage of ... However, SPIs gels of stored grains had high values of hardness. The values cohesiveness and elasticity of the SPIs gels did ...
http://repositorio.unicamp.br/jspui/handle/REPOSIP/256399
*  Patent US8492107 - Neural proteins as biomarkers for nervous system injury and other neural ... - Google Patents
... heparin chromatography, affinity chromatography, sequential extraction, gel electrophoresis and liquid chromatography. The ... For example, spots can be excised from the gel and analyzed by gas phase ion spectrometry. Alternatively, the gel containing ... heparin chromatography also reduces the complexity of a sample and separates markers according to their binding characteristics ... hydroxyapatite chromatography, gel exclusion chromatography, gel electrophoresis, dialysis, or affinity chromatography. ...
http://www.google.ca/patents/US8492107